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Nevin Manimala Statistics

Sustainable removal of caffeine and acetaminophen from water using biomass waste-derived activated carbon: Synthesis, characterization, and modelling

Chemosphere. 2024 Mar 23:141787. doi: 10.1016/j.chemosphere.2024.141787. Online ahead of print.

ABSTRACT

The removal of caffeine (CFN) and acetaminophen (ACT) from water using low-cost activated carbons prepared from artichoke leaves (AAC) and pomegranate peels (PAC) was reported in this paper. These activated carbons were characterized using various analytical techniques. The results showed that AAC and PAC had surface areas of 1203 and 1095 m2 g-1, respectively. The prepared adsorbents were tested for the adsorption of these pharmaceuticals in single and binary solutions. These experiments were performed under different operating conditions to evaluate the adsorption properties of these adsorbents to remove CFN and ACT. AAC and PAC showed maximum adsorption capacities of 290.86 and 258.98 mg g-1 for CFN removal, 281.18 and 154.99 mg g-1 for the ACT removal over a wide pH range. The experimental equilibrium adsorption data fitted to the Langmuir model and the kinetic data were correlated with the pseudo-second order model. AAC showed the best adsorption capacities for the removal of these pharmaceuticals in single systems and, consequently, it was tested for the simultaneous removal of these pollutants in binary solutions. The simultaneous adsorption of these compounds on AAC was improved using the central composite design and response surface methodology. The results indicated an antagonistic effect of CFN on the ACT adsorption. AAC regeneration was also analyzed and discussed. A statistical physics model was applied to describe the adsorption orientation of the tested pollutants on both activated carbon samples. It was concluded that AAC is a promising adsorbent for the removal of emerging pollutants due to its low cost and reusability properties.

PMID:38527633 | DOI:10.1016/j.chemosphere.2024.141787

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Nevin Manimala Statistics

No Clinical Difference in Outcomes Between Inlay and Onlay Arthroscopic Biceps Tenodesis Techniques during Rotator Cuff Repair

J Shoulder Elbow Surg. 2024 Mar 23:S1058-2746(24)00195-2. doi: 10.1016/j.jse.2024.03.007. Online ahead of print.

ABSTRACT

BACKGROUND: Both inlay and onlay arthroscopic biceps tenodesis (ABT) are common procedures performed during rotator cuff repair. The inlay method involves creating a bone socket in the bicipital groove to secure the long head of the biceps tendon (LHBT) using an interference screw. The onlay method utilizes a suture anchor to secure the LHBT on the surface of the bicipital groove. Little is known on the long-term differences in patient reported outcomes (PROs) between these two techniques. The primary purpose of this study was to compare PROs of inlay versus onlay ABT with a minimum follow-up of two years. Secondary aims were to evaluate the impact of rotator cuff tear size on outcomes and compare rates of complications between the two techniques.

METHODS: A retrospective chart review was performed to identify patients who had an ABT during a full-thickness rotator cuff repair. Any symptom specific to the biceps were noted, including pain and cramping, Popeye deformity, or revision surgery. Complication rates were compared between groups. The VAS pain score, ASES, SANE, and VR-12 scores were compared at two years. The impact of rotator cuff tear size was analyzed by categorizing into small/medium or large/massive based on operative reports and arthroscopic images.

RESULTS: There were 165 patients identified (106 in the inlay group and 59 in the onlay group). No revision surgeries were performed secondary to the biceps tendon in either group. Eleven patients (10%) in the inlay group complained of biceps pain or cramping compared to 2 patients (3%) in the onlay group (p = .11). One Popeye deformity was noted in each group (p = .67). No significant differences were found between groups for VAS (p=.41), ASES functional (p = .61), ASES Index (p = .91), SANE (p = .09), VR-12 PCS (p = .77), or VR-12 MCS (p = .09). Rotator cuff tear size within the groups also did not demonstrate statistical significance.

CONCLUSION: No clinical differences or complications were found at minimum two-year follow-up between inlay and onlay arthroscopic biceps tenodesis in patients undergoing rotator cuff repair when controlling for tear size. The clinical relevance suggests either technique is effective and can be based on surgeon preference.

PMID:38527622 | DOI:10.1016/j.jse.2024.03.007

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Nevin Manimala Statistics

Electrodiagnostic Severity Does Not Predict Short to Mid-term Outcomes of Cubital Tunnel Release Surgery

J Shoulder Elbow Surg. 2024 Mar 23:S1058-2746(24)00216-7. doi: 10.1016/j.jse.2024.01.055. Online ahead of print.

ABSTRACT

HYPOTHESIS: This study aimed to explore the prognostic value of electrodiagnostic studies (EDS) to clarify their utility in clinical practice prior to cubital tunnel release surgery as well as identify patient factors associated with patient-reported functional improvement after surgery. Our hypothesis is that patients with severe preoperative findings on EDS will tend to experience less functional improvement after surgery given the extent of ulnar nerve compressive injury.

METHODS: Patients with cubital tunnel syndrome and preoperative electrodiagnostic data treated from 2012-2022 with cubital tunnel release were assessed regarding demographic information, preoperative physical exam findings, EDS findings, postoperative complications, and patient-reported outcomes. Short to mid-term qDASH scores were collected for all patients for further evaluation of preoperative EDS data. Patients were grouped into those who had met the MCID in delta qDASH at short to mid-term follow-up and those that did not. EDS data included sensory nerve onset latency, peak latency, amplitude, conduction velocity as well as motor nerve latency, velocity, and amplitude. Electromyogram (EMG) studies were also reviewed which included data pertaining to fibrillations, presence of abnormal fasciculation, positive sharp waves, variation in insertional activity, motor unit activity, duration of activity, and presence of increasing polymorphisms.

RESULTS: Of the 257 patients included, 160 (62.0%) were found to meet the MCID for short to mid-term qDASH scores. There were no significant differences between patients who did or did not meet the MCID regarding baseline demographics, comorbidities, preoperative exam findings, and operative technique. Patients who met MCID tended to have lower complication (3.80% vs 7.20%; p=0.248) and revision (0.60% vs 4.10%; p=0.069) rates but these findings were not statistically significant. The cubital tunnel severity as determined by the EDS was similar between cohorts (14.1% vs. 14.3%, p=0.498). Analysis of EMG testing showed there were no significant differences in preoperative, short to mid-term qDASH, or delta short to mid-term qDASH scores for patients with or without abnormal EMG findings. Multivariate regression suggested that only age (p=0.003) was associated with larger delta qDASH scores (Table 7).

CONCLUSION: Patient-reported preoperative disease severity may predict the expected postoperative change in ulnar nerve functional improvement and EDS may not have prognostic value for patients undergoing cubital tunnel decompression. Therefore, physicians may suggest surgical treatment without positive EDS findings and still expect postoperative improvement in functional outcomes.

PMID:38527621 | DOI:10.1016/j.jse.2024.01.055

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The Prevalence of Incontinence and Its Association With Urinary Tract Infections, Dermatitis, Slips and Falls, and Behavioral Disturbances Among Older Adults in Medicare Fee-for-Service

J Wound Ostomy Continence Nurs. 2024 Mar-Apr 01;51(2):138-145. doi: 10.1097/WON.0000000000001054.

ABSTRACT

PURPOSE: The purpose of this study was to examine the prevalence of urinary (UI), fecal (FI), and dual incontinence (DI) in older adults and their association with urinary tract infections, dermatitis, slips and falls, and behavioral disturbances based on Medicare fee-for-service (FFS) claims data.

DESIGN: Retrospective analysis.

SUBJECTS AND SETTINGS: Data from administrative claims from the CMS Medicare Limited Data Set (5% sample) for all months in 2018 were reviewed. The analysis was limited to FFS Medicare beneficiaries, with minimum of 3-month enrollment in Parts A and B who were at least 65 years old. This cohort included 1.2 million beneficiaries in the United States.

METHODS: We used diagnosis codes to identify members with incontinence and grouped these members into 3 categories (UI only, FI only, and DI). We also divided claims based on 4 sites of care (nursing home, skilled nursing facility, home health, and self- or family care). We then determined the prevalence of (1) urinary tract infections (UTIs), (2) dermatitis, (3) slips and falls, and (4) behavioral disturbances for each type of incontinence.

RESULTS: We found that 11.2% of Medicare members had a claims-based diagnosis of incontinence in 2018. On average, those diagnosed with incontinence experienced 5 times more UTIs, 2 times as many dermatitis events, more than twice as many slips and falls, and 2.8 times more behavior disturbances compared to those without an incontinence diagnosis. For those with DI, the prevalence of the 4 outcomes was significantly higher (between 22% and 185%) compared to those with UI only.

CONCLUSIONS: Findings show that Medicare beneficiaries diagnosed as incontinent experience a much higher prevalence of UTIs, dermatitis, slips and falls, and behavioral disturbances compared to those without a diagnosis of incontinence. Our results suggest that incontinence may be an important indicator diagnosis for multiple other conditions and, if not well-managed, may challenge the desire for those who are incontinent to age at home.

PMID:38527324 | DOI:10.1097/WON.0000000000001054

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Evaluation of 5′-tRF-His-GTG As a Molecular Biomarker in Breast Cancer Diagnoses and Prognosis

Cancer Biother Radiopharm. 2024 Mar 25. doi: 10.1089/cbr.2023.0048. Online ahead of print.

ABSTRACT

Background: Breast cancer (BC) is the most prevalent cancer among women worldwide. Although advances have been made in the identification of predictive biomarkers, current options for early diagnosis and prognostic analysis are still suboptimal. Recently, transfer-RNA-derived RNA fragments (tRFs) have emerged as a class of small noncoding RNAs that play a role in the cancer progression. The authors aimed to identify a specific class of tRFs as a molecular marker for BC diagnosis and prognosis in clinical management. Methods: The levels of 5′-tRF-His-GTG were quantified in BC tissue (n = 101) and inflammatory normal breast tissue (n = 22) using in situ hybridization. Clinicopathological parameters were obtained, including age, tumor node metastasis stage, hormone receptor status, histopathological grade, lymphovascular invasion, and recurrence. The correlation between the expression of 5′-tRF-His-GTG and these parameters in different BC subtypes was analyzed. Patient death and cancer progression were regarded as clinical endpoints in the survival analysis. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were also performed to predict the involvement in pivotal biological process. Results: The expression of 5′-tRF-His-GTG was significantly downregulated in BC tissues and was in connection with T stage in human epidermal growth factor 2-positive and basal-like BC, as well as N stage and histopathological grade in luminal BC. Patients with low expression of 5′-tRF-His-GTG had a poor overall survival rate. Statistics of GO and KEGG pathway revealed that cation channel activity, protein catabolic process, response to temperature stimulus, cell cycle, focal adhesion, and glycerophospholipid metabolism were significantly enriched. Conclusions: This study suggests that the assessment of 5′-tRF-His-GTG expression could serve as a novel biomarker for individual diagnosis and prognosis in BC.

PMID:38527246 | DOI:10.1089/cbr.2023.0048

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Nevin Manimala Statistics

Improved RNA stability estimation through Bayesian modeling reveals most Salmonella transcripts have subminute half-lives

Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308814121. doi: 10.1073/pnas.2308814121. Epub 2024 Mar 25.

ABSTRACT

RNA decay is a crucial mechanism for regulating gene expression in response to environmental stresses. In bacteria, RNA-binding proteins (RBPs) are known to be involved in posttranscriptional regulation, but their global impact on RNA half-lives has not been extensively studied. To shed light on the role of the major RBPs ProQ and CspC/E in maintaining RNA stability, we performed RNA sequencing of Salmonella enterica over a time course following treatment with the transcription initiation inhibitor rifampicin (RIF-seq) in the presence and absence of these RBPs. We developed a hierarchical Bayesian model that corrects for confounding factors in rifampicin RNA stability assays and enables us to identify differentially decaying transcripts transcriptome-wide. Our analysis revealed that the median RNA half-life in Salmonella in early stationary phase is less than 1 min, a third of previous estimates. We found that over half of the 500 most long-lived transcripts are bound by at least one major RBP, suggesting a general role for RBPs in shaping the transcriptome. Integrating differential stability estimates with cross-linking and immunoprecipitation followed by RNA sequencing (CLIP-seq) revealed that approximately 30% of transcripts with ProQ binding sites and more than 40% with CspC/E binding sites in coding or 3′ untranslated regions decay differentially in the absence of the respective RBP. Analysis of differentially destabilized transcripts identified a role for ProQ in the oxidative stress response. Our findings provide insights into posttranscriptional regulation by ProQ and CspC/E, and the importance of RBPs in regulating gene expression.

PMID:38527194 | DOI:10.1073/pnas.2308814121

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Low-Dose Radiation Associated Mortality Risks of Site-Specific Solid Tumors in U.S. Shipyard Workers

J Occup Environ Med. 2024 Mar 25. doi: 10.1097/JOM.0000000000003099. Online ahead of print.

ABSTRACT

BACKGROUND: U.S. nuclear capable shipyard workers have increased potential for occupational radiation exposure.

OBJECTIVE: to examine solid tumor mortality risks at low doses.

METHOD: 437,937 workers working from 1957 to 2004 at eight U.S. shipyards were studied.

RESULTS: Radiation workers with a median life-time dose at 0.82 mSv had a significantly lower solid tumor mortality risk [Relative risk (RR): 0.96, 95% confidence interval (CI): 0.94-0.98] than non-radiation workers. Among 153,930 radiation workers., the RRs of solid tumors increased with increasing dose categories without statistical significance. The dose category >0- < 25 mSv had significantly lower RR (0.95, 95% CI: 0.91-0.99) vs. 0 dose and the Excess Relative Risk was 0.05/100 mSv (95% CI: 0.01-0.08).

CONCLUSION: Solid tumor risk might increase with radiation dose, but not linearly at low doses. Actual mortality risk may be dependent on dose received.

PMID:38527177 | DOI:10.1097/JOM.0000000000003099

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Nevin Manimala Statistics

Quality, Reliability, and Readability of Online Information on Idiopathic Intracranial Hypertension

J Neuroophthalmol. 2024 Mar 25. doi: 10.1097/WNO.0000000000002130. Online ahead of print.

ABSTRACT

BACKGROUND: To evaluate the quality & reliability, technical quality, and readability of patient-targeted online information on idiopathic intracranial hypertension.

METHODS: In this cross-sectional study, we searched Google and Bing search engines for the terms “idiopathic intracranial hypertension” and “pseudotumor cerebri.” We evaluated the first 50 search outcomes for each search term. Peer-reviewed articles, targeted advertisements, book chapters, videos, personal blogs, websites intended for healthcare professionals, and non-English websites were excluded. Websites were classified as institutional or private. Two independent reviewers assessed each website for 1) quality and reliability using DISCERN, Health on the Net Code of Conduct (HONcode), and JAMA criteria and 2) technical quality based on 11 criteria. Readability was evaluated using 6 measures (Flesch-Kincaid Reading Ease score and grade level, Gunning Fog, Simple Measure of Gobbledygook (Smog) index, Coleman-Liau index, and automated readability index).

RESULTS: Sixty-three websites (37 institutional) were included. The mean scores for the DISCERN, HONcode, and JAMA criteria were 3.6 ± 1 (1-4.8; 1: worse, 5: best), 10.3 ± 2.8 (2-16; 0: worse, 16: best), and 2 ± 1.3 (0-4; 0: worse, 4: best), respectively. The mean technical quality score was 0.8 ± 0.1 (0.5-1). The mean Flesch-Kincaid grade level score was 8.9 ± 1.8 (3.3-13.3). For Flesch-Kincaid grade level, 47 (74.6%) websites scored a grade level of 10 or less. For Gunning Fog Score, 35 websites (55.6%) scored from 7.3 to 10.8. For the Smog Index, 46 (73%) websites scored 5.7-8. The mean Coleman Liau Index was 16 ± 2.1 (9.6-22.2). For the automated readability index, 30 (50.7%) websites scored less than the eighth grade. No statistically significant difference was present between institutional and private websites except for JAMA, which scored higher in private websites (2.4 vs 1.7, P = 0.03).

CONCLUSIONS: Our findings suggest that online information on idiopathic intracranial hypertension/pseudotumor cerebri for patients generally demonstrates moderate to good quality and reliability. Although most websites maintain credible technical quality, the readability exceeds recommended levels for the average American reader. This may impede understanding, emphasizing the need for future improvements.

PMID:38527126 | DOI:10.1097/WNO.0000000000002130

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Nevin Manimala Statistics

Access to specialized health care services among older Canadians

Health Rep. 2024 Mar 20;35(3):18-32. doi: 10.25318/82-003-x202400300002-eng.

ABSTRACT

BACKGROUND: Canada is experiencing rapid population aging, which has a wide range of implications, including an increased need for health care services. However, very few studies have examined use of specialized health care services (e.g., visits to medical specialists, non-emergency tests, and surgeries) among older Canadians.

DATA AND METHODS: Data from the Canadian Health Survey on Seniors – 2019/2020 were used to examine specialized health care service use among older Canadians. Latent class analysis was calibrated using a nationally representative sample of 39,047 Canadians aged 65 years or older to identify distinct patterns of need factors related to health care service use. Multivariable logistic regression, stratified by gender, was used to examine the association of predisposing characteristics, enabling resources, and need factors with specialized health care service use.

RESULTS: In 2019/2020, an estimated 2.6 million older Canadians (43.4%) visited medical specialists, 1.4 million (23.2%) got non-emergency tests, and 0.6 million (10.4%) had non-emergency surgeries. Among those, 15.6% reported experiencing difficulties accessing services. Women were less likely than men to have visited medical specialists and have received non-emergency tests. Lower education was consistently associated with lower odds of specialized health care service use. Individuals in the multimorbidity, high stress-multimorbidity-disability, and poor physical and mental health classes were more likely than those in the comparatively healthy class to use specialized health care services and to experience difficulties accessing them.

INTERPRETATION: Findings of this study highlight gender differences and the importance of considering multidimensional need factors – ranging from physical health to mental health to psychosocial factors – in examining use of specialized health care services.

PMID:38527108 | DOI:10.25318/82-003-x202400300002-eng

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Exploring the use of experimental small area estimates to examine the relationship between individual-level and area-level community belonging and self-rated health

Health Rep. 2024 Mar 20;35(3):3-17. doi: 10.25318/82-003-x202400300001-eng.

ABSTRACT

BACKGROUND: Small area estimation refers to statistical modelling procedures that leverage information or “borrow strength” from other sources or variables. This is done to enhance the reliability of estimates of characteristics or outcomes for areas that do not contain sufficient sample sizes to provide disaggregated estimates of adequate precision and reliability. There is growing interest in secondary research applications for small area estimates (SAEs). However, it is crucial to assess the analytic value of these estimates when used as proxies for individual-level characteristics or as distinct measures that offer insights at the area level. This study assessed novel area-level community belonging measures derived using small area estimation and examined associations with individual-level measures of community belonging and self-rated health.

DATA AND METHODS: SAEs of community belonging within census tracts produced from the 2016-2019 cycles of the Canadian Community Health Survey (CCHS) were merged with respondent data from the 2020 CCHS. Multinomial logistic regression models were run between area-level SAEs, individual-level sense of community belonging, and self-rated health on the study sample of people aged 18 years and older.

RESULTS: Area-level community belonging was associated with individual-level community belonging, even after adjusting for individual-level sociodemographic characteristics, despite limited agreement between individual- and area-level measures. Living in a neighbourhood with low community belonging was associated with higher odds of reporting being in fair or poor health, versus being in very good or excellent health (odds ratio: 1.53; 95% confidence interval: 1.22, 1.91), even after adjusting for other factors such as individual-level sense of community belonging, which was also associated with self-rated health.

INTERPRETATION: Area-level and individual-level sense of community belonging were independently associated with self-rated health. The novel SAEs of community belonging can be used as distinct measures of neighbourhood-level community belonging and should be understood as complementary to, rather than proxies for, individual-level measures of community belonging.

PMID:38527107 | DOI:10.25318/82-003-x202400300001-eng