Luminescence. 2021 Nov 5. doi: 10.1002/bio.4154. Online ahead of print.
ABSTRACT
In this paper, two simple, rapid and highly sensitive spectrofluorimetric methods were developed and validated for nystatin determination in its pure form and pharmaceutical dosage form (oral suspension). The first method is based on measuring of nystatin native fluorescence after dilution with isopropyl alcohol at 407 nm (excitation 303 nm). The floursence intensity is linered dependant on the nystatin concentrartion within the specified range 50-500 ngmL-1 . The second is based on micellar enhancement of nystatin fluoescence by using sodium dodecyl sulphate (SDS). In the presence of 2% w/v SDS, about 1.9 -fold enhancement can be achieved in the relative fluorescence intensity (RFI) of nystatin. The linear range for the second method was 20-100 ngmL-1 . The limit of quantification and detection were found to be 43.23 ngmL-1 and 14.27 ngmL-1 (method I), 6.08 ngmL-1 and 2.0 ngmL-1 (method II). According to percentage recoveries and relative standard deviations (RSDs) obtained, the proposed methods are precise (RSDs were less than 2%), reproducible, and accurate and could be successfully applied for quantitative estimation of nystatin in its dosage form. The statistical results of this method were compared with that of the reported method and showed excellent agreement in respect for accuracy and precision.
PMID:34738720 | DOI:10.1002/bio.4154
