Pol Merkur Lekarski. 2021 Oct 22;49(293):346-351.
ABSTRACT
It is known that the violation of one or more functions of the liver, where the basic biochemical processes take place, is reflected in the functional state of many organs and systems, causing severe consequences. For the effective treatment of the hepatobiliary system diseases the drugs from fungi and plant materials are promising, the ingredients of which are close to natural metabolites, have different mechanisms of hepatoprotective action and, in general, can have a positive effect on liver function.
AIM: The aim of the research was to investigate the hepatoprotective properties of the Maitake mushrooms thick extract in the experiment on rats with paracetamol (acetaminophen)-induced hepatitis.
MATERIALS AND METHODS: The study was performed on 60 white male rats weighing 180-210 g and aged 6-6,5 months. Rats were divided into 10 groups, each of which included 6 animals. Acute hepatitis was simulated by acetaminophen intragastrically administering at a dose of 1250 mg/ kg of body weight 1 time per day as a suspension in 2% starch gel solution for 2 days. Correction of the toxic lesions was performed with a thick extract of Maitake mushrooms, which was administered intragastrically 2 hours before the introduction of acetaminophen and daily after the lesion at a dose of 150 mg/kg of body weight. “Silibor” (active basis – silymarin) was chosen as a comparison drug, which was administered according to the same scheme as Maitake extract at a dose of 20 mg/kg of animal body weight. On the 3rd, 7th and 10th days from the onset of the lesion, rats were euthanized using sodium barbamyl. Liver homogenate and blood serum were tested. Blood was taken from the hearts of animals. Endogenous intoxication of animals after the introduction of corrective factors was assessed by the activity of ALT, AST, GGTP, LF and the size of the thymol sample. All changes were confirmed by parametric and nonparametric methods of statistical analysis of the results of the study.
RESULTS: The expressed cytolysis of hepatocytes, after administration to rats of toxicant, on the basis of research of the activity of aminotransferases, gamma-glutamyltranspeptidase, alkaline phosphatase and thymol sample size is proved. The results of the experiment were confirmed histologically. The introduction of a thick extract of Maitake mushrooms contributed to the normalization of the studied indicators.
CONCLUSIONS: The application of the Maitake mushrooms thick extract as a corrective factor in the simulated acetaminophen hepatitis indicates its hepato-, cytoprotective and antioxidative properties.
PMID:34800021
