Biomed Chromatogr. 2021 Nov 26:e5285. doi: 10.1002/bmc.5285. Online ahead of print.
ABSTRACT
Bisphenol-A (BPA) is a high-production-volume industrial chemical mainly used in the production of polycarbonates and epoxy resins utilized in the manufacture of containers, bottles,toys, and medical devices. It has systemic effects as an endocrine-disruptor even at low-doses. In order to analyse the amount at biological materials sensitive and reproducible methods have to be used. Different doses and duration (90μg/L and 900μg/L, 24 hours, 120 hours and 21 days) of BPA exposure to whole body zebrafish was analysed after the specific homogenization of tissue, followed by a modified method by HPLC. The mobile phase was acetonitrile and water with a gradient method of reverse-phase C18 column and ex=227nm/em=313nm. The calibration curve for BPA in HPLC-FLD method was between 1-1000ng/mL concentration range, linear and r2 =0.999. The mean and standard error of mean values (4.29±1.05, 2.50±0.92, 2.53±0.68) for control, and (10.43±2.61, 11.46±3.24, 8.55±3.11) for BPA-90μg/L and (17.78±4.39, 21.55±4.37, 25.32±3.25) for BPA-900μg/L (24 hours, 120 hours and 21 days respectively). Although some statistical significance among dose/time was observed between two different dose treated groups, statistical significance was not found in dose/time results within the group. However, the positive result of BPA in the control group can be explained by low-dose, chronic exposure or prevalence of EDC substances.
PMID:34826884 | DOI:10.1002/bmc.5285
