Transbound Emerg Dis. 2021 Dec 16. doi: 10.1111/tbed.14424. Online ahead of print.
ABSTRACT
African swine fever virus (ASFV) is a global threat to swine production and sustainable pork supply. Without a commercially available vaccine, prevention of ASFV entry and spread is reliant on biosecurity and early detection of infection. Although ASFV ingestion in swill or feed by naïve pigs is a likely route of initial introduction, controlled experimental studies rarely utilize natural consumption as the infection route. In the current study, we utilized biological samples collected from pigs 5 days after natural consumption of ASFV in feed and liquid to assess diagnostic sensitivity for early detection of virus infection. Biological samples (serum, spleen, lymph nodes, tonsils, and feces) were assessed for the presence of ASFV using quantitative PCR and virus isolation. Statistical methods modeled the detection sensitivity of each sample type with each diagnostic assay in individual samples. Our results provide important information that can be incorporated into ASFV surveillance programs. This article is protected by copyright. All rights reserved.
PMID:34914859 | DOI:10.1111/tbed.14424
