Luminescence. 2022 Feb 10. doi: 10.1002/bio.4206. Online ahead of print.
ABSTRACT
Approved, straightforward, fast and delicate spectrofluorimetric strategy has been developed for the estimation of tepotinib (TEPO), sotorasib (SOTO) and darolutamide (DARO) as a new antineoplastic drugs. Spectrofluorimetric strategy was based on quantitative fluorescence quenching of MER at 538 nm after being excited at 350 nm by the addition of cited drugs in presence of acetate buffer (pH 3.5). The degree of fluorescence quenching is directly proportional to the concentrations of the cited drugs within the concentration range of 0.5-10.0, 0.2-10 and 0.4-10.0 μg mL-1 for TEPO, SOTO and DARO, respectively. Mean ± S.D. were calculated for the studied drugs as follows; 99.9±0.87, 99.72±1.08 and 100.21±1.44, for TEPO, SOTO and DARO, respectively. LOD values were 0.16, 0.05 and 0.11 μg mL-1 while LOQ values were 0.5, 0.15 and 0.36 μg mL-1 for TEPO, SOTO and DARO, respectively. Statistical comparison of comes about with those gotten by detailed strategies given great understanding and uncovered that there were no noteworthy contrasts in exactness and exactness between strategies. The proposed strategy was connected effectively to analyze measurement shapes containing the examined drugs. Moreover, the recommended fluorimetric strategy was connected for examination of TEPO, SOTO and DARO in human plasma and urine test.
PMID:35142060 | DOI:10.1002/bio.4206
