Cytopathology. 2022 Mar 27. doi: 10.1111/cyt.13121. Online ahead of print.
ABSTRACT
OBJECTIVE: Evaluate the performance of different DNA image cytometry (DNA-ICM) ploidy parameters to categorize a DNA-ICM result, and consequently identify high-grade cervical intraepithelial neoplasia or worse (≥CIN2).
METHODS: Cervical samples from 232 women were collected for DNA-ICM analysis and biopsy confirmation. Five DNA parameters were used to define DNA aneuploidy: number of cells with exceeding events (EE) over 2.5cEE, 4cEE, 5cEE, 9cEE, and aneuploid stemlines. DNA-ICM results were categorized as normal, suspicious, and abnormal.
RESULTS: For individual DNA ploidy parameters, sensitivity for 50 cells with 2.5cEE, 45 cells with 4cEE, 1 cell with 9cEE and aneuploid stemline were 72.95%. 54.1%, 69.67% and 54.1%, while specificity were 80.0%, 90.0%, 89.09% and 95.45%, respectively. For 5cEE parameter, sensitivity for 1, 2, 3, 4 and 5 cells were 93.44%, 85.25%, 81.97%, 77.87% and 75.41%, while specificity were 46.36%, 63.64%, 74.55%, 76.36% and 80.91%, respectively. For categorized DNA-ICM results, a suspicious result revealed superior sensitivity to an abnormal result (87.70% vs 82.79%, P = 0.031), but inferior specificity (54.55% vs 75.45%, P <0.001). Both DNA-ICM results were statistically different from a normal result (P <0.05).
CONCLUSION: For prognostic purposes 1 cell with 9cEE, 45 cells with 4cEE and aneuploid stemline are the best parameters to categorize an abnormal DNA-ICM result, followed by 50 cells with 2.5cEE and 4 cells with 5cEE. For screening purposes, 10 cells with 2.5cEE, 10 cells with 4cEE, and 2 cells with 5cEE are suitable parameters to categorize a suspicious DNA-ICM result.
PMID:35340056 | DOI:10.1111/cyt.13121
