Plant Cell Environ. 2022 Apr 21. doi: 10.1111/pce.14337. Online ahead of print.
ABSTRACT
Canola varieties exhibit discernible variation in drought avoidance and drought escape traits, reflecting adaptation to water-deficit environments. Our understanding of underlying genes and their interaction across environments in improving crop productivity is limited. A doubled haploid (DH) population was analysed to identify QTL associated with water use efficiency (WUE) related traits. High WUE in the vegetative phase was associated with low seed yield. Based on the resequenced parental genome data, we developed sequence-capture-based markers and validated their linkage with carbon isotope discrimination (Δ13 C) in an F2 population. mRNA-Seq was performed to determine the expression of candidate genes underlying Δ13 C QTL. QTL contributing to main and QTL × Environment interaction effects for Δ13 C and yield were identified. One multiple-trait QTL for Δ13 C, days to flower, plant height, and seed yield was identified on chromosome A09. Interestingly, this QTL region overlapped with a homoeologous exchange event (HE), suggesting its association with the multiple traits. Transcriptome analysis revealed 121 significantly differentially expressed genes underlying Δ13 C QTL on A09 and C09, including in HE regions. Sorting out the negative relation between vegetative WUE and seed yield is a priority. Genetic and genomic resources and knowledge so developed could improve canola WUE and yield. This article is protected by copyright. All rights reserved.
PMID:35445756 | DOI:10.1111/pce.14337
