Proteomics Clin Appl. 2022 Apr 24:e2100049. doi: 10.1002/prca.202100049. Online ahead of print.
ABSTRACT
PURPOSE: The study aim is a comparative proteome-based analysis of different autologous bone entities (alveolar bone (AB), iliac cortical bone (IC) and iliac spongiosa (IS)) used for alveolar onlay grafting.
EXPERIMENTAL DESIGN: Site-matched bone samples of AB, IC and IS were harvested during alveolar onlay grafting. Proteins were extracted using a detergent-based (sodium dodecyl sulfate) strategy and trypsinized. Proteome analysis was performed using liquid chromatography – tandem mass spectrometry (LC-MS/MS). MaxQuant was used for peptide-to-spectrum matching, peak detection, and quantitation. Linear models for microarray analysis (LIMMA) were used to detect differentially abundant peptides and proteins.
RESULTS: 1730 different proteins were identified across the 15 samples at a false discovery rate of 1 %. Partial least-squares discriminant analysis approved segregation of AB, IC and IS protein profiles. LIMMA statistics highlighted 66 proteins that were more abundant in AB then in IC (vs. 92 proteins were enriched in IC over AB). Gene ontology enrichment analysis revealed a matrisomal vs. an immune-related proteome fingerprint in AB vs IC.
CONCLUSION AND CLINICAL RELEVANCE: This pilot study demonstrates an ECM protein-related proteome fingerprint in AB and an immune-related proteome fingerprint in IS and IC. This article is protected by copyright. All rights reserved.
PMID:35462455 | DOI:10.1002/prca.202100049
