Lett Appl Microbiol. 2022 May 25. doi: 10.1111/lam.13746. Online ahead of print.
ABSTRACT
β-cyclodextrin glucosyltransferase (β-CGTase) is an essential enzyme to catalyze the biotransformation of starch into β-cyclodextrins (β-CD). β-CD has widespread applications in the biomedical, pharmaceutical, and food industries. The present study focused on β-CGTase production using an efficient natural microbial strain and statistical production optimization for enhanced production. The isolated organism Bacillus sp. NCIM 5799 was found to be 5μm short bacilli under FE-SEM and alkalihalophilic in nature. The β-CGTase production was optimized using a combination of Plackett-Burman design (PBD) and Central Composite Design – Response Surface Methodology (CCD-RSM). On PBD screening Na2 CO3 , peptone, and MgSO4 .7H2 O were found to be significant for optimal β-CGTase production, whereas the soluble starch and K2 HPO4 concentrations were found to be non-significant for β-CGTase production. The significant factors obtained after PBD were further optimized using CCD-RSM design. Peptone was found to have a significant interaction effect with Na2 CO3 , and MgSO4 .7H2 O and Na2 CO3 exhibited significant effect on production of CGTase. The production of β-CGTase was enhanced in the presence of peptone (3%) and Na2 CO3 (0.8%). CGTase production obtained was 156.76 U/ml when optimized using CCD-RSM. The final optimized medium (RSM) shows 7.7 and 5.4 fold high production as compared to un-optimized and one factor at a time production media.
PMID:35611566 | DOI:10.1111/lam.13746
