J Endod. 2023 Jul 8:S0099-2399(23)00391-6. doi: 10.1016/j.joen.2023.07.002. Online ahead of print.
ABSTRACT
INTRODUCTION: This study evaluated the effects of diabetes mellitus (DM) on the nanostructure of root canal dentin using high-resolution transmission electron microscopy (HRTEM) and inductively coupled plasma mass spectrometry (ICP-MS).
METHODS: Twenty extracted human premolars from diabetic and non-diabetic patients (n=10 in each group) were decoronated and sectioned horizontally into forty 2-mm-thick dentin discs, with each disc designated for a specific test. ICP-MS was used to determine the different elemental levels of Cu, Li, Zn, Se, Sr, Mn, and Mg in diabetic and non-diabetic specimens. HRTEM was used to analyze the shape and quantity of the apatite crystals in diabetic and non-diabetic dentin at the nanostructural level. Statistical analysis was performed using Kolmogorov-Smirnov and student’s t-test (p<0.05).
RESULTS: ICP-MS revealed significant differences in trace element concentrations between the diabetic and non-diabetic specimens (p<0.05), with lower levels of Mg, Zn, Sr, Li, Mn, and Se (p<0.05), and higher levels of Cu in diabetic specimens (p<0.05). HRTEM revealed that diabetic dentin exhibited a less compact structure with smaller crystallites and significantly more crystals in the 2500 nm2 area (p<0.05).
CONCLUSION: Diabetic dentin exhibited smaller crystallites and altered elemental levels than non-diabetic dentin, which could explain the higher root canal treatment failure rate in diabetic patients.
PMID:37429496 | DOI:10.1016/j.joen.2023.07.002
