Odontology. 2025 Nov 10. doi: 10.1007/s10266-025-01256-5. Online ahead of print.
ABSTRACT
This study evaluated the effects of three chelating agents-glycolic acid (GA), ethylenediaminetetraacetic acid (EDTA), and etidronic acid (HEDP)-combined with different irrigation activation techniques on the pushout bond strength of Biodentine to root dentin after calcium hydroxide removal. A total of 120 extracted single-rooted mandibular premolars were randomly assigned to nine experimental and one control group (n = 12). Following Ca(OH)2 dressing and incubation, canals were irrigated with GA, EDTA, or HEDP, each activated using passive ultrasonic irrigation (PUI), XP-Endo Finisher (XPF), or conventional needle irrigation (CNI). Biodentine was placed into 2 mm-thick root slices, and pushout bond strength was measured using a universal testing machine. Statistical analysis was performed using one-way ANOVA for group comparisons, and two-way ANOVA was used to evaluate factor effects (α = 0.05). Overall, GA produced higher bond strength than EDTA (p = 0.027). The difference between GA and HEDP was not significant under PUI activation; however, GA yielded significantly higher bond strength than HEDP when activated with CNI or XPF. Using 10% GA with PUI enhanced Biodentine-dentin adhesion, suggesting its potential as a biocompatible and effective alternative to conventional chelators in regenerative endodontic treatments, improving coronal sealing and long-term stability.
PMID:41212455 | DOI:10.1007/s10266-025-01256-5
