Zhongguo Zhong Yao Za Zhi. 2025 Oct;50(20):5706-5716. doi: 10.19540/j.cnki.cjcmm.20250604.101.
ABSTRACT
To analyze the difference of overall metabolites in cultivated and wild Rehmannia glutinosa, this study used cultivated and wild R. glutinosa from different locations as research objects. Their metabolites were detected using liquid chromatography-mass spectrometry(LC-MS), and multivariate statistical analysis was adopted to analyze metabolites. Differential metabolites were identified, and differential metabolic pathways were determined based on the Kyoto Encyclopedia of Genes and Genomes(KEGG) database. The results showcased that 1 802 metabolites were detected, and the metabolites of cultivated and wild R. glutinosa were similar, including 13 kinds of compounds such as terpenoids, phenolic acids, lipids, and alkaloids. Overall, the relative content of metabolites of wild R. glutinosa was higher than that of cultivated R. glutinosa. There were 665 differential metabolites between cultivated and wild R. glutinosa. A total of 157 differential metabolites were up-regulated, and 508 differential metabolites were down-regulated. According to the KEGG database, 93 metabolites with significant differences were annotated, with phenolic acids representing the highest proportion. There were a total of 55 metabolic pathways corresponding to differential metabolites, which were divided into three categories: metabolism, genetic information processing, and environmental information processing. Significantly different pathways included flavonoid biosynthesis(ko00941), phenylpropane biosynthesis(ko00940), and pyrimidine metabolism(ko00240), all of which were associated with plant metabolism. The study found that the metabolite species of wild and cultivated R. glutinosa were consistent, whereas the overall metabolite content of wild R. glutinosa was higher than that of cultivated R. glutinosa, and the difference in their pharmacological effects remained to be further investigated.
PMID:41508202 | DOI:10.19540/j.cnki.cjcmm.20250604.101
