Indian J Gastroenterol. 2026 Apr 8. doi: 10.1007/s12664-026-01967-9. Online ahead of print.
ABSTRACT
BACKGROUND: Gallbladder cancer (GBC) is the most common biliary tract malignancy and a leading cause for mortality in the Asian sub-continent. The low survival rate is attributed to current invasive diagnostic methods and late-stage disease detection. The aim of this study was to estimate the potential value of miR 183_5p and 3651 as diagnostic blood-based biomarkers in GBC patients.
METHODS: This single center observational study evaluates differential expression of miRNAs in GBC and normal gallbladder tissues via micro-array analysis. The level of selected oncogenic miRNAS were detected in 130 individuals comprising GBC patients, Gallbladder stone (GBS) and healthy controls by using qRT-PCR. The diagnostic value of miR-183-5p and 3651 in GBC was evaluated and compared with the tumor markers carcinoembryonic antigen (CEA) and carbohydrate antigen 19.9 (CA 19.9).
RESULTS: As many as 45 differentially expressed microRNAs (p < 0.05) were determined, of which 23 were upregulated and 22 were down regulated. miR 183_5p (2.5-fold) and 3651 (5.6-fold) were significantly over expressed in GBC patients compared to controls (p < 0.05). The area under the curve (AUC) value of miR 183_5p and 3651 for GBC diagnosis was 0.684 and 0.752, respectively, which was more valuable than those including CEA (0.617) and CA19.9 (0.718).
CONCLUSION: Study underscores ability of serum miRNAs 183_5p and 3651 as diagnostic biomarkers for early GBC detection.
PMID:41949739 | DOI:10.1007/s12664-026-01967-9
