J AOAC Int. 2021 Dec 17:qsab165. doi: 10.1093/jaoacint/qsab165. Online ahead of print.
ABSTRACT
BACKGROUND: The NEMIS Technologies N-LightTM L. monocytogenes assay utilizes chemiluminescence designed for the qualitative detection of Listeria monocytogenes from environmental surface samples.
OBJECTIVE: To validate the NEMIS Technologies N-LightTM L. monocytogenes assay as part of the AOAC Performance Tested MethodSM Program for the detection of L. monocytogenes on stainless steel, plastic (polystyrene) and ceramic environmental surfaces.
METHODS: Using the Vitl Life Science Solutions Lu-mini luminometer, the NEMIS Technologies N-LightTM L. monocytogenes assay was compared to the ISO 11290-1:2017: Microbiology of the Food Chain-Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. – Part 1 using a 1″ x 1″ stainless steel test area in an unpaired study design.
RESULTS: The NEMIS Technologies N-LightTM L. monocytogenes assay using the Vitl Life Science Solutions Lu-mini luminometer demonstrated no statistically significant differences between presumptive and confirmed results or between candidate and reference method results. Data for additional PTM studies met acceptance criteria requirements.
CONCLUSIONS: The NEMIS Technologies N-LightTM L. monocytogenes assay is an effective method for the qualitative detection of L. monocytogenes from stainless steel, plastic (polystyrene) and ceramic environmental surface samples.
HIGHLIGHTS: The NEMIS method is the first chemiluminescence detection system based on a novel, patented, dioxetane compound.
PMID:34919698 | DOI:10.1093/jaoacint/qsab165
