Georgian Med News. 2022 Nov;(332):44-55.
ABSTRACT
In spite of all clinical manifestation of undifferentiated dysplasia of connective tissue (UDCT) including joint hypermobility syndrome still there are no exact morphological characteristics of structural articular cartilage changes on the background of UDCT.
THE AIM: to determine the structural peculiarities of articular cartilage reactive changes in rats with an experimental undifferentiated dysplasia of connective tissue.
STUDY DESIGN: animal experience.
METHODS: The object of the study was 162 knee joints of white laboratory rats, which were divided into 3 groups: – The 1st one – intact animals; the 2nd group consisted of experimental animals, each of which at the 18th day of fetal development was injected by 0.05 ml of antigen in 0.9% NaCl; the 3rd group consisted of control rats, injected by 0.05 ml of 0.9% NaCl at the 18th day of dated pregnancy. Morphological structure of articular cartilage of knee joints was examined at days 1st, 11th, 14th, 21st, 30th, 45th, 60th, 90th, 120th after birth. Fixation of histological material was carried out in 10% neutral formaldehyde. Histochemical, histological methods, statistic methods were used in the work.
RESULTS: Experiencing aggressive exposure from osteoblasts of the subchondral bone, along with increasing mechanical stress, the articular cartilage of rats with experimental UDCT, first compensatory turns thicker, and then irreversibly thin down, which is a prerequisite for the development of primary osteoarthrosis.
CONCLUSIONS: In rats with experimental UDCT the articular cartilage is isolated on the 11th day during the formation of the subchondral bone, in contrast to control rats in which articular cartilage differentiates on the 14th day after birth. In rats with experimental UDCT, the content of sulfated glycosaminoglycans in the matrix of the articular cartilage decreases. There is an accelerated replacement of the deep zone of the articular cartilage by the subchondral bone and the thinning of the articular cartilage on the 90th day after birth.
PMID:36701776
