Int J Periodontics Restorative Dent. 2023 Nov 8;0(0):1-21. doi: 10.11607/prd.6598. Online ahead of print.
ABSTRACT
PURPOSE: This in vivo study in a canine model assessed the effect of recombinant human platelet-derived growth factor (rhPDGF) on healing of periapical tissues following apical surgery.
MATERIALS AND METHODS: Sixty-four premolar teeth of six 2-year-old beagle dogs were randomly assigned to 4 experimental groups; 16 teeth each. In each tooth, coronal access was done, and the pulp extirpated. The teeth were then left open to the oral cavity for one week and then sealed with IRM for 8 weeks. Nonsurgical endodontic treatment (NSRCT) was then performed. A split-mouth design was used, and intra-animal side randomization was applied to the 4 groups as follows: Group 1) Apical curettage +1.5 mm root-end resection; Group 2) Apicoectomy + mineral trioxide aggregate (MTA) root-end filling; Group 3) Apicoectomy + MTA root-end filling + rhPDGF; and Group 4) Apical curettage + rhPDGF. The animals were sacrificed 24 months following apical surgery and histological and μCT analyses were performed for bone volume loss (BVL).
RESULTS: Group 1 showed partial resolution of the periapical lesions but without signs of tissue regeneration. The BVL was 49.09 ± 10.97 mm3. Group 2 showed reformation of cementum in 9 out of 16 teeth. No direct attachment between the newly formed cementum and the MTA were observed. Bone regeneration was minimal and the BVL was 35.34 ± 10.97 mm3. Group 3 showed regeneration of all damaged apical tissues but without direct contact between the cementum and MTA. The BLV was 4.51± 1.55 mm3. Group 4 showed regeneration of PDL, bone, cementum, and attachment of functional cementum fibers was observed. The BVL for this group was 2.82 ± 2.3 mm3. The difference in BVL was statistically significant only for Group 1 and Group 2 (P<0.05). There was no significance difference in the BVL between Group 3 and Group 4.
CONCLUSIONS: Recombinant human platelet-derived growth factor may play a role in regeneration of apical tissue structures following apical surgery.
CLINICAL RELEVANCE: Oral healthcare providers should be aware that addition of recombinant human platelet-derived growth factor may positively impact the regeneration of periapical tissues following apical surgery.
PMID:37939277 | DOI:10.11607/prd.6598
