STAR Protoc. 2024 Mar 9;5(1):102926. doi: 10.1016/j.xpro.2024.102926. Online ahead of print.
ABSTRACT
Here, we present a protocol for the identification of differentially expressed genes through RNA sequencing analysis. Starting with FASTQ files from public datasets, this protocol leverages RumBall within a self-contained Docker system. We describe the steps for software setup, obtaining data, read mapping, sample normalization, statistical modeling, and gene ontology enrichment. We then detail procedures for interpreting results with plots and tables. RumBall internally utilizes popular tools, ensuring a comprehensive understanding of the analysis process.
PMID:38461412 | DOI:10.1016/j.xpro.2024.102926
