Virol J. 2025 Nov 12;22(1):373. doi: 10.1186/s12985-025-02999-x.
ABSTRACT
PURPOSE: Present study aimed to compare the boiling and magnetic bead nucleic acid extraction method for HPV genotypes detection.
METHODS: By mixing hemoglobin with HPV positive quality control products, the anti-interference ability of two nucleic acid extraction methods on hemoglobin was simulated. Subsequently, on the basis of paired small sample and longitudinal large sample comparisons, the HPV detection rates between the boiling and magnetic bead-based method were evaluated. Finally, the cost-benefit comparisons of the two extraction methods were further evaluated. A two-sided P value of less than 0.05 was considered statistically significant.
RESULTS: As for boiling method, when the hemoglobin concentration exceeded 30 g/L, HPV positive control could not be detected, nevertheless, the HPV positive control can still be detected even when the hemoglobin concentration reached 60 g/L for magnetic bead method. In the paired small-scale (639 cases) experiment, results indicated that the positive detection rate of HPV using the magnetic bead method was significantly higher than that of the boiling method, with positive rates of 20.66% and 10.02% (P < 0.001), respectively. Additionally, in the longitudinal large-scale analysis (16540 cases) reached the same conclusion. Compared with boiling method, the cost of magnetic bead method increased by 13.14%, however, the detection rate of HPV increased by 106.19%.
CONCLUSION: Compared to the boiling method, the magnetic bead-based nucleic acid extraction technique exhibited superior anti-interference capabilities and a significant higher detection rate for HPV. Furthermore, it is highly cost-effective. It is anticipated that the magnetic bead method will be fully adopted for HPV detection in place of the boiling method in the future. Of course, more research is needed to verify this conclusion.
PMID:41225522 | DOI:10.1186/s12985-025-02999-x
