J Med Virol. 2021 Jul 30. doi: 10.1002/jmv.27248. Online ahead of print.
ABSTRACT
Understanding the regional lineages and sublineages of HPV 56 would be of great importance for further evolutionary, epidemiological, and biological investigations. To identify the distribution of lineages and sublineages of HPV 56 in Iran, the sequence variations of E6 gene were analyzed in normal, premalignant, and malignant samples obtained from the cervix. In total, 58 HPV 56-positive samples were investigated by nested-PCR and followed by bidirectional direct nucleotide sequencing analysis. Both lineages A and B were identified in studied samples. Lineage B was dominant as it was detected in 88.4% of all samples and the remaining of samples were belonged to lineages A (11.6%). Sublineages A1 and A2 were detected in 3.3 % and 8.3 % of all samples, respectively. Regard to the pathological stages of cervical specimens, no statistically significant differences was found in three studied groups (P > 0.05). In conclusion, our findings showed that lineage B of HPV 56 was prevalent in Iran. However, further studies with larger sample size are warranted to estimate the pathogenicity risk of HPV 56 lineages/sublineages to progress of cervical cancer among Iranian women. This article is protected by copyright. All rights reserved.
PMID:34329490 | DOI:10.1002/jmv.27248
