BMC Oral Health. 2026 Feb 6. doi: 10.1186/s12903-026-07837-2. Online ahead of print.
ABSTRACT
BACKGROUND: To investigated the combined effect of adhesive type and film thickness on dentin bond strength by comparing one and two-step universal adhesives applied in single and double layers.
METHODS: Forty caries-free human third molars were collected and standardized Class I cavities were prepared. Specimens were restored using one-step (Clearfil Tri-S Bond Universal, Japan) and two-step (G2-Bond Universal, Japan) universal adhesive systems. Each group was subdivided into single and double-layer applications, light-cured, and restored with a universal composite resin. Sticks with a cross-sectional 1 × 1 mm² were prepared for testing. Adhesive film thickness was measured under a stereomicroscope at ×80 magnification. Micro-tensile bond strength (µTBS) was evaluated either immediately or after 5000 thermocycles (TC). Failure modes were assessed under ×40 magnification. Wilcoxon Signed Ranks Test was used for paired comparisons of immediate and after TC tensile bond strengths. Mann-Whitney U test compared single vs. double layers of the adhesives. For adhesive film thickness, Independent Samples t-test was applied for group comparisons (p < 0.05).
RESULTS: Double-layer application produced significantly thicker adhesive films for Clearfil Tri-S Bond and G2-Bond Universal compared with single-layer application (p < 0.05). However, no significant differences in µTBS were observed between single and double-layer groups of either adhesive, regardless of aging. Thermocycling significantly reduced bond strength for both systems (p < 0.05). Overall, the two-step adhesive demonstrated higher µTBS values than the one-step adhesive.
CONCLUSION: Although double-layer application increased adhesive film thickness, it did not improve dentin bond strength. The two-step universal adhesive system showed superior bonding performance compared with the one-step system, both immediately and after thermocycling.
PMID:41652397 | DOI:10.1186/s12903-026-07837-2
