Mol Biol Evol. 2026 Mar 9:msag061. doi: 10.1093/molbev/msag061. Online ahead of print.
ABSTRACT
piqtree (pronounced pie-cue-tree) is an easy to use, open-source Python package that provides Python script based control of IQ-TREE’s phylogenetic inference engine. piqtree builds IQ-TREE as a Python package, presenting a library of Python functions for performing many of IQ-TREE’s capabilities including phylogenetic reconstruction, ultrafast bootstrapping, branch length optimization, model selection, rapid neighbor-joining, alignment simulation, and more. As piqtree explicitly uses IQ-TREE’s phylogenetic algorithms, the computational and statistical performance of piqtree equal that of IQ-TREE. Modestly higher memory usage may be expected owing to the Python runtime and the need to load the alignment in Python. By exposing IQ-TREE’s algorithms within Python, piqtree offers users a greatly simplified experience in development of phylogenetic workflows through seamless interoperability with other Python libraries and tools mediated by the cogent3 package. It enables users to perform interactive phylogenetic analyses and visualization using, for instance, Jupyter notebooks. We present the key features available in the piqtree library and a small case study that showcases its interoperability and highlight its potential for linking a high performance phylogenetic inference engine with more user friendly interfaces. piqtree is distributed for use as a standard Python package at https://pypi.org/project/piqtree/, documentation is available at https://piqtree.readthedocs.io, user contributed solutions at https://github.com/cogent3/c3codeshare, help forums at https://github.com/iqtree/piqtree/discussions and source code at https://github.com/iqtree/piqtree.
PMID:41802268 | DOI:10.1093/molbev/msag061
