Front Cell Dev Biol. 2026 Feb 26;13:1695908. doi: 10.3389/fcell.2025.1695908. eCollection 2025.
ABSTRACT
BACKGROUND: To investigate the effect of platelet-rich-fibrin (PRF) on the healing of seawater-impregnated wounds in rabbits and to explore the mechanism.
MATERIAL AND METHODS: Twenty-four New Zealand White rabbits were used. Two full-thickness skin wound models were created on each rabbit’s back and immersed in seawater. According to a random number table, one wound on each rabbit was assigned to the PRF group (treated with PRF gel) and the contralateral wound served as the control (untreated). Wound healing rate, histomorphology, bacteriology, and neovascularization (via CD34 immunohistochemistry) were assessed on days 1, 4, 7, and 14 post-operation. Statistical analysis was performed using ANOVA with paired samples t-test and Bonferroni correction.
RESULTS: Wounds in the control group exhibited significant erythema, edema, and inflammatory exudate, with a healing rate of only 53.5% ± 3.2% by day 14. In contrast, PRF-treated wounds showed minimal signs of infection, reduced inflammation, and were almost completely healed (92.9% ± 0.9%) by day 14. The wound healing rate was significantly higher in the PRF group at all time points (P < 0.01). Bacteriological analysis identified BacAutologous PRF significantly promotes the healing of seawater-immersed wounds in rabbits. The mechanism is likely multifactorial, involving the promotion of angiogenesis, reduction of inflammation, and potential inhibition of bacterial growth. PRF represents a promising therapeutic option for the management of seawater immersion wounds. d formation of new capillaries and fibroblasts. Immunohistochemistry confirmed a significantly higher density of CD34+ neovessels in the PRF group at days 4, 7, and 14 (P < 0.05).
CONCLUSION: Autologous PRF significantly promotes the healing of seawater-immersed wounds in rabbits. The mechanism is likely multifactorial, involving the promotion of angiogenesis, reduction of inflammation, and potential inhibition of bacterial growth. PRF represents a promising therapeutic option for the management of seawater immersion wounds.
PMID:41835809 | PMC:PMC12979441 | DOI:10.3389/fcell.2025.1695908
