Category: Nevin Manimala

J Hematop. 2025 Jul 1;18(1):28. doi: 10.1007/s12308-025-00643-9.

ABSTRACT

Given its strong correlation with disease progression and risk stratification, CD200 has emerged as a pivotal biomarker in chronic lymphocytic leukemia (CLL). Elevated CD200 expression, strongly linked to CLL progression, underscores its diagnostic and therapeutic potential. In this case-control study, we evaluated CD200 expression levels in CLL patients and analyzed their correlation with key clinicopathological features, investigating its potential as a critical biomarker for diagnosis and follow-up strategies. Peripheral blood samples from 27 CLL patients and five healthy individuals were stained by fluorochrome-conjugated antibodies against CD19 and CD200 markers, followed by flow cytometry. Data analysis compared CD200 expression levels among CLL patients at four stages and in the healthy control group. CD200 expression levels in CLL patients’ lymphocytes significantly exceeded those observed in the healthy control group (P < 0.0001). Within the patient group, expression levels progressively increased from low-risk to high-risk classifications, with statistically significant differences between each category (P < 0.0001). Furthermore, a strong positive association was identified between CD200 expression and disease clinical stage (r = 0.758, P < 0.0001), WBC count (r = 0.705, P < 0.0001), and lymphocyte percentage (r = 0.544, P = 0.009). Conversely, a strong inverse correlation was observed with neutrophil count (r = – 0.55, P = 0.008). Overall, CD200 assessment may serve as a valuable prognostic marker in CLL, providing insight into disease progression and aiding in treatment monitoring.

PMID:40593283 | DOI:10.1007/s12308-025-00643-9

Sci Rep. 2025 Jul 2;15(1):23107. doi: 10.1038/s41598-025-07621-2.

ABSTRACT

This study investigates the yield stability and adaptability of sixteen open-pollinated tomato (Solanum lycopersicum Mill.) genotypes across multiple environments in the Kashmir Valley, employing Additive Main Effect and Multiplicative Interaction (AMMI) analysis to examine genotype-environment interactions (GEI). A Randomized Complete Block Design (RCBD) with three replications was implemented at six locations over two years, representing the region’s diverse environmental conditions. Analysis of variance indicated that the effects of environment (E), genotype (G), and GEI were all highly significant (p < 0.001) in influencing yield per hectare, with the environment contributing 47.5% of the total variation, underscoring the impact of local conditions on performance. Key stability indicators, including Weighted Average of Absolute Scores (WAAS) and Multi-Trait Stability Index (MTSI), assessed genotype stability and yield, with Arka Meghali and NDF-9 emerging as top-performing varieties across locations. Arka Meghali achieved the highest yield, while NDF-9 showed remarkable adaptability. The consistent rankings provided by stability indices reinforced the reliability of WAAS and MTSI as selection tools in multi-environment trials. Based on multi-environment stability analysis, Arka Meghali and NDF-9 were identified as superior open-pollinated tomato genotypes combining high yield and stability across diverse environments. These varieties are recommended for cultivation in the Kashmir Valley to enhance tomato productivity under variable agro-climatic conditions.These findings underscore the utility of GEI analysis in identifying tomato genotypes with robust yield and stability, providing valuable insights for breeding programs and crop management in ecologically sensitive regions like the Northern Himalayas. This research establishes a foundation for future studies on environmental adaptability in crop improvement, highlighting the potential of selected genotypes for sustainable cultivation in Kashmir.

PMID:40593274 | DOI:10.1038/s41598-025-07621-2

Sci Rep. 2025 Jul 1;15(1):21909. doi: 10.1038/s41598-025-07988-2.

ABSTRACT

This study proposes a novel hypothesis exploring the potential relationship between magnetite nanoparticle sizes in the human brain and neural oscillation frequencies. Magnetite, a naturally occurring magnetic material found in brain tissue, has been the subject of increasing scientific interest due to its potential role in brain function and its possible link to neurodegenerative diseases. Concurrently, neural oscillations are known to play crucial roles in various cognitive processes. Our theoretical model, grounded in Néel’s theory of superparamagnetism and principles of electromagnetism, suggests a direct physical relationship between specific magnetite grain sizes (19-24 nm) and a wide range of neural oscillation frequency bands (1-1000 Hz). Using computational simulations and statistical analyses, we investigated how the magnetic properties of these nanoparticles might interact with or influence neural activity. Our calculations show that magnetite particles within this size range have magnetic moment fluctuation frequencies that span the range of known neural oscillations, with larger particles corresponding to lower frequencies and smaller particles to higher frequencies, following Néel’s relaxation equation. This relationship encompasses the entire spectrum of known neural oscillations, from delta waves to high-frequency oscillations. Of particular interest, we found that magnetite particles within this size range could potentially interact with the 50-60 Hz frequencies of power grid systems, raising intriguing questions about potential interactions between environmental electromagnetic fields and endogenous brain activity. These results suggest potential size-dependent interactions between magnetite particles and neural oscillations, with implications for understanding brain function, aging processes, and the impact of environmental electromagnetic fields. This work provides a theoretical approach for future experimental studies and may offer new perspectives on the complex dynamics of brain physiology and pathology.

PMID:40593272 | DOI:10.1038/s41598-025-07988-2

Sci Rep. 2025 Jul 2;15(1):22972. doi: 10.1038/s41598-025-07453-0.

ABSTRACT

The objective of this study was to evaluate the effect of adding of C-Type Natriuretic (CNP) to the in vitro culture medium of bovine embryos on cryotolerability through modulation of lipid content and profile, as well as modulation of gene transcripts linked to embryonic metabolism. Initially, a concentration of 400 nM of CNP was used throughout the in vitro culture and blastocysts were collected for lipid content analysis by Sudan Black B. In addition, blastocysts were selected by morphological quality and developmental stage, and the samples collected were analyzed using MRM- profiling. After, blastocysts were vitrified using OPS. Subsequently the warming, hatched blastocysts were collected and evaluated for transcript abundance in a microfluidic platform. Differences of probabilities lower than P < 0.05, and/or fold change ˃1.5 were considered significant. The CNP group presented a reduction in the relative abundance of ions belonging to different lipid subclasses, such as acylcarnitine, sphingomyelin, cholesteryl esters, free fatty acids, and glycerophospholipid. Furthermore, the triacylglycerol lipids TG 52:3 NL 16:1, TG 56:3 NL 18:1, and the glycerophospholipid C22:6, were increased in the CNP group. A modulation of blastocyst transcripts was also observed by increased transcription of ATF4, and a trend statistical significance of BMP15 and GFPT2 transcripts. There was no difference in blastocyst development rates after warming of CNP-treated embryos.

PMID:40593265 | DOI:10.1038/s41598-025-07453-0

Commun Med (Lond). 2025 Jul 1;5(1):247. doi: 10.1038/s43856-025-00887-7.

ABSTRACT

BACKGROUND: Understanding the role of space weather, specifically Geomagnetic Disturbances (GMDs) caused by solar activity, on health outcomes is unclear. One emerging link includes the impact of space weather on myocardial infarctions (MI). In this study we examined the correlation between MI and GMDs in Brazil.

METHODS: We used a database from the public health in Brazil, focusing on the city of São José dos Campos (23° 10′ 44″ S, 45° 53′ 13″ W), located in the state of São Paulo, during the period of 1998-2005. We focused on admissions for MIs, which included a total of 871 men and 469 women. We categorized the MI data into three age groups: age 30 and younger, age 31-60, and age over 60. Additionally, we incorporated Planetary Index (Kp) data as an indicator of variations in the Earth’s geomagnetic field resulting from solar disturbances, categorized as quiet, moderate, or disturbed days. In our analysis, we employed two methods: statistical counting and the unsupervised clustering known as K-Means, considering the attributes of age, sex, and geomagnetic condition.

RESULTS: Here we show that geomagnetic conditions have an impact on MI cases, particularly for women. The rate of relative frequency of MI cases during disturbed geomagnetic conditions is almost three times greater compared to quiet geomagnetic conditions. Using the unsupervised K-Means algorithm, the results indicate that the group associated with disturbed geomagnetic conditions has a higher incidence of MIs in women.

CONCLUSIONS: Overall, our results provide evidence that women may exhibit a higher susceptibility to the effects of geomagnetic disturbances caused by solar activity on MI.

PMID:40593256 | DOI:10.1038/s43856-025-00887-7

Sci Rep. 2025 Jul 2;15(1):23057. doi: 10.1038/s41598-025-07100-8.

ABSTRACT

Allergic rhinitis (AR) is a nasal inflammation triggered by the immune system’s response to airborne allergens, with white blood cells playing a crucial role in the development of allergic symptoms. This study aimed to investigate the genetic correlations between AR and various blood traits in European and East Asian populations using linkage disequilibrium score regression (LDSC). By leveraging GWAS summary statistics, we identified significant genetic overlap between AR and eosinophil counts in both populations. Cross-trait analysis revealed 52 pleiotropic loci associated with AR and eosinophil counts in Europeans, while 12 novel loci were discovered in East Asians. Among these, five loci, including IL1RL1 and IL4R, were shared between Europeans and East Asians. Additionally, we identified a novel East Asian-specific locus near the CD28 gene. Differential gene expression analysis further showed that CD28 expression was significantly lower in AR patients compared to healthy controls, suggesting its potential involvement in AR pathogenesis. These findings underscore the utility of multi-trait GWAS in uncovering pleiotropic loci and provide new insights into the genetic architecture of AR, particularly emphasizing population-specific loci like CD28. This research opens avenues for understanding the genetic basis of AR and developing targeted therapies.

PMID:40593228 | DOI:10.1038/s41598-025-07100-8

Sci Rep. 2025 Jul 1;15(1):22203. doi: 10.1038/s41598-025-07663-6.

ABSTRACT

This study investigated corneal epithelial thickness and its distribution in keratoconus (KC) and forme fruste keratoconus (FFK). A total of 35 KC eyes, 25 FFK eyes, and 35 control eyes underwent anterior segment-optical coherence tomography for corneal epithelial evaluation. Sixty KC eyes were included for the assessment of epithelial changes according to KC severity. Epithelial thickness in the central, paracentral, and peripheral areas-each subdivided into eight zones-was statistically analysed. Minimum, maximum, and average epithelial thicknesses were reduced in KC eyes, especially in the inferior and inferior-temporal zones. Standard deviations were greater in KC eyes across nearly all zones. In FFK and KC eyes, the differences between superior and inferior epithelial thickness were significantly altered compared to control eyes. According to the Amsler-Krumeich classification, more advanced KC stages were associated with greater corneal thinning. Epithelial heterogeneity decreased in the central and paracentral area as a result of superior epithelial thinning, whereas it increased in the peripheral area with KC progression. Epithelial thickness and distribution were altered in KC and even in FFK eyes with normal topography. Different stages of KC may exhibit distinct epithelial remodelling patterns.

PMID:40593226 | DOI:10.1038/s41598-025-07663-6

Clin Oral Investig. 2025 Jul 2;29(7):366. doi: 10.1007/s00784-025-06429-8.

ABSTRACT

OBJECTIVES: Fluoride-based cariostatic agents are commonly used in dental products and are generally considered safe. However, ongoing discussions about potential adverse effects are driving research into alternative agents, such as lanthanide salts. This study aims to evaluate the cytotoxic effects of different cariostatic agents, including fluoride compounds (NaF, Na₂PO₃F, NH₄F) and lanthanide salts (Ce(NO₃)₃, CeCl₃, Sm(NO₃)₃, SmCl₃).

MATERIALS AND METHODS: Mouse fibroblasts (L-929) were cultured in Eagle’s Minimum Essential Medium supplemented with 5% fetal bovine serum and 1% penicillin-streptomycin. Cell viability was assessed via MTT assay after 24 h of exposure to test compounds at concentrations of 0.0128, 0.064, 0.32, 1.6, 8, 40, 200 and 1000 mM, and lactate dehydrogenase (LDH) release was quantified to assess membrane integrity. The accumulation of reactive oxygen species (ROS) was determined after 24 h. Data were analyzed by non-parametric methods at a significance level of α = 0.05 (Mann-Whitney U and Kruskal-Wallis tests).

RESULTS: Cell viability decreased significantly for NaCl and NaNO₃ at 200 mM, and for NaF, NH₄F, Ce(NO₃)₃, CeCl₃, Sm(NO₃)₃ and SmCl₃ at 1.6 mM, falling below 70% of control (P ≤ 0.0178). Similarly, LDH assays indicated a significant incline in cytotoxicity at a concentration of 200 mM for NaCl, NaNO₃ and Na₂PO₃F, and at 8 mM for NaF, NH₄F and all lanthanide compounds (P ≤ 0.0016). ROS quantification showed that NaF, NH₄F, CeCl₃, Sm(NO₃)₃ and SmCl₃ induced oxidative stress at 1.6 mM with statistical significance (P ≤ 0.0065).

CONCLUSIONS: Fluoride and lanthanide compounds exhibited similar in vitro biocompatibility, comparable to that of table salt.

CLINICAL RELEVANCE: Both fluoride- and lanthanide-based cariostatic agents appear to pose a low biological risk to surrounding oral tissues when used at appropriate doses in dental products.

PMID:40593225 | DOI:10.1007/s00784-025-06429-8

Sci Rep. 2025 Jul 1;15(1):21153. doi: 10.1038/s41598-025-07878-7.

ABSTRACT

The aim of this retrospective study was to analyze the efficacy and safety of lateral abdominal wall suspension during surgery compared with traditional procedures in patients with pelvic organ prolapse without uterine preservation. Collect the data of patients with uterine and vaginal wall prolapse who underwent surgery from January 2022 to December 2023. All patients were divided into three groups according to different surgical procedures: Study Group (laparoscopic total hysterectomy + bilateral salpingectomy/oophorectomy + lateral abdominal wall suspension + posterior colporrhaphy), Control Group 1 (laparoscopic-assisted vaginal total hysterectomy + bilateral salpingectomy/bilateral oophorectomy + anterior and posterior colporrhaphy), and Control Group 2 (vaginal total hysterectomy + anterior and posterior colporrhaphy).Statistically analyze the clinical indicators and recurrence situations in different Groups. Patients in Study group exhibited significantly higher preoperative POP-Q staging for pelvic organ prolapse severity(P < 0.01), longer operative times(P = 0.01, P < 0.01), but significantly reduced intraoperative blood loss(P = 0.01, P < 0.01)and shorter postoperative urinary catheterization duration(P = 0.01, P < 0.01) compared to the other two groups. No differences were detected in postoperative infection, vaginal bleeding or duration of hospitalization(P > 0.05). However, stress incontinence in 1-year after operation has no difference between study group and control groups. Recurrence rates in Control Group 1 is higher than Study Group (P = 0.02), While, it has no difference between Study Group and Control Group 2. Lateral abdominal wall suspension, despite a longer operative duration, surpasses traditional surgeries in minimizing intraoperative blood loss, cutting down the time of postoperative urinary catheter retention, and lowering the postoperative recurrence rate. To firmly establish the long-term curative effects and safety of lateral abdominal wall suspension, future research with larger sample sizes and longer follow-up periods is essential.

PMID:40593197 | DOI:10.1038/s41598-025-07878-7

Sci Rep. 2025 Jul 1;15(1):21554. doi: 10.1038/s41598-025-07890-x.

ABSTRACT

In this study, we investigated the status quo of illness acceptance, community self-efficacy, and social isolation in elderly patients with Parkinson’s disease (PD). We explored the effects of illness acceptance and community self-efficacy on the relationship between social isolation and loneliness in patients with PD based on the self-regulation theory model. We selected elderly patients with PD from the outpatient department of two Grade iii-A hospitals in Guangdong Province using convenience sampling. We collected data using a general information questionnaire. We used the Lubben Social Network Scale, UCLA Loneliness Scale, Community’s Self-Efficacy Scale, and Acceptance of Illness Scale. Data were analyzed using descriptive statistics, Pearson’s correlation coefficient, and bootstrap sampling to test the multiple mediation model. We recruited 142 elderly patients with PD. Their mean age was 69.30 years (standard deviation = 5.34, range from 60 to 89 years). Approximately 55.6% (n = 79) of the patients were male. The mean scores for community self-efficacy, social network, and illness acceptance were 11.03 ± 6.21, 13.29 ± 5.4, and 23.56 ± 6.871, respectively. Loneliness was negatively associated with illness acceptance (r = -0.536, p < 0.001) and community self-efficacy (r = -0.654, p < 0.001). The results of multiple mediation analysis indicated that illness acceptance (effect: -0.172, 95% confidence interval (CI) [-0.297, 0.067]) and community self-efficacy (effect: -0.271, 95% CI [-0.446, -0.115]) individually and collectively influenced the relationship between social isolation and loneliness (effect: -0.055, 95% CI [-0.105, -0.017]). The direct effect and mediating effect values accounted for 59.6% and 40.4% of the total effect value, respectively. Illness acceptance and community self-efficacy mediate the association between social isolation and loneliness. Interventions that help facilitate mutuality, illness acceptance, and community self-efficacy may help minimize loneliness in patients with PD.

PMID:40593192 | DOI:10.1038/s41598-025-07890-x