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Nevin Manimala Statistics

DIGE Analysis of Clinical Specimens

Methods Mol Biol. 2023;2596:177-199. doi: 10.1007/978-1-0716-2831-7_14.

ABSTRACT

Two-dimensional difference gel electrophoresis (2D-DIGE) is an elegant gel electrophoretic analytical tool for comparative protein assessment. It is based on two-dimensional gel electrophoresis (2D-GE) separation of fluorescently labeled protein extracts. The tagging procedures are designed to not interfere with the chemical properties of proteins with respect to their pI and electrophoretic mobility, once a proper labeling protocol is followed. The use of an internal pooled standard makes 2D-DIGE a highly accurate quantitative method enabling multiple protein samples to be separated on the same two-dimensional gel. Technical limitations of this technique (i.e., underrating of low abundant, high molecular mass and integral membrane proteins) are counterbalanced by the incomparable separation power which allows proteoforms and unknown PTM (posttranslational modification) identification. Moreover, the image matching and cross-gel statistical analysis generates robust quantitative results making data validation by independent technologies successful.

PMID:36378440 | DOI:10.1007/978-1-0716-2831-7_14

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Nevin Manimala Statistics

Investigating the Effect of Return-to-Play Timing After Injury on Performance: Does the Analysis Answer the Research Objective?

Sports Med. 2022 Nov 15. doi: 10.1007/s40279-022-01792-y. Online ahead of print.

ABSTRACT

Return-to-play decision making should be based on all the advantages and disadvantages of return to play for athletes, not just the risk of injury. For competitive athletes, this includes the effect of early versus delayed return to sport on performance. In this paper, we address the questions “How can I estimate the effect of injury on the individual’s performance at return to play?” and “What is the effect of delaying return to sport on the individual’s performance?”. To address these questions, we describe (1) some foundational concepts, design and analytical challenges related to estimating the causal effect of return to play timing on performance in the athlete, (2) additional challenges if one is interested in the effects of delaying return to play and (3) differences when the questions relate to the team’s performance. Although the analytical strategies described appear complicated, coaches and athletes make these judgements informally every day without explicitly stating their assumptions. Using a formal approach should help analysts provide the most valid answers to the questions asked by athletes and coaches. In brief, the choice of a comparison group depends on the research question and requires that one consider the hypothetical performance trajectory of the athlete had they never been injured. Thus, the optimal comparison group depends on the shape of the expected trajectory and the specific research question being asked.

PMID:36378413 | DOI:10.1007/s40279-022-01792-y

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Nevin Manimala Statistics

Potentially inappropriate prescribing in older adults with cancer receiving specialist palliative care: a retrospective observational study

Int J Clin Pharm. 2022 Nov 15. doi: 10.1007/s11096-022-01506-4. Online ahead of print.

ABSTRACT

BACKGROUND: Older adults (≥ 65 years) with cancer receiving palliative care often have other health conditions requiring multiple medications.

AIM: To describe and assess the appropriateness of prescribing for older adults with cancer in the last seven days of life in an inpatient palliative care setting.

METHOD: Retrospective observational study of medical records for 180 patients (60.6% male; median age: 74 years; range 65-94 years) over a two-year period. Medication appropriateness was assessed using: STOPPFrail, OncPal deprescribing guideline and criteria for identifying Potentially Inappropriate Prescribing in older adults with Cancer receiving Palliative Care (PIP-CPC).

RESULTS: 94.5% of patients had at least one other health condition (median 3, IQR 2-5). The median number of medications increased from five (IQR 3-7) seven days before death, to 11 medications on the day of death (IQR 9-15). The prevalence of PIP varied depending on the tool used: STOPPFrail (version 1: 17.2%, version 2: 19.4%), OncPal (12.8%), PIP-CPC (30%). However, the retrospective nature of the study limited the applicability of the tools. Increasing number of medications had a statistically significant effect on risk of PIP across all tools (STOPPFrail (version 1: 1.29 (1.13-1.37), version 2: 1.30 (1.16-1.48)); OncPal 1.13 (1.01-1.27); PIP-CPC 0.70 (0.61-0.82)).

CONCLUSION: This study found that the number of medications prescribed to older adults with cancer increased as time to death approached, and the prevalence of PIP varied with the application of different tools. The study also highlights the difficulties of examining PIP in this patient cohort.

PMID:36378404 | DOI:10.1007/s11096-022-01506-4

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Nevin Manimala Statistics

The impact of performance feedback reports on physician ordering behavior in the use of computed tomography pulmonary angiography (CTPA)

Emerg Radiol. 2022 Nov 15. doi: 10.1007/s10140-022-02100-x. Online ahead of print.

ABSTRACT

PURPOSE: The increased utilization, and potential overutilization, of computed tomography pulmonary angiography (CTPA) is a well-recognized issue within emergency departments (EDs). The objective of this study is to determine the impact of performance feedback reports on CTPA ordering behavior among ED physicians.

METHODS: We conducted a prospective study of the impact of individualized performance feedback reports on the ordering behavior of physicians working at two high-volume community EDs in Ontario, Canada. We generated individualized reports (or “Dashboards”) for each ED physician containing detailed feedback and peer comparison for each physician’s CTPA ordering. Our baseline pre-intervention period was January 1 to December 31, 2018, and our intervention period was January 1, 2019, to December 31, 2021. We tracked individual and group ordering behavior through the study period. Our primary outcomes are impact of feedback on (1) overall group ordering rate and (2) overall diagnostic yield. Secondary analysis was done to determine the impact of the intervention on those physicians with the highest CTPA utilization rate.

RESULTS: There was no statistically significant difference in the diagnostic yield of the included physicians in either of the years of the intervention period. There was a statically significant increase in the utilization rate for CTPA from 2018 to 2020 and 2021 from 5.9 to 7.9 and 11.4 CTPAs per 1000 ED visits respectively (p < 0.5).

CONCLUSION: Our study found no consistent significant impact of individualized feedback and peer comparison on physician ordering of CTPAs. This points to a potentially greater impact of environmental and institutional factors, as opposed to physician-targeted quality improvement measures, on physician ordering behavior.

PMID:36378395 | DOI:10.1007/s10140-022-02100-x

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Copper, lead and zinc interactions during phytoextraction using Acer platanoides L.-a pot trial

Environ Sci Pollut Res Int. 2022 Nov 15. doi: 10.1007/s11356-022-23966-x. Online ahead of print.

ABSTRACT

Of the many environmental factors that modulate the phytoextraction of elements, little has been learnt about the role of metal interactions. The study aimed to show how different concentrations of Cu, Pb and Zn in the cultivation medium influenced the biomass, plant development and phytoextraction abilities of Acer platanoides L. seedlings. Additionally, the impact on the content and distribution of Ca, K, Mg and Na in plant parts was studied with an analysis of phenols. Plants treated with a mixture of two metals were characterised by lower biomass of leaves and higher major elements content jointly than those grown in the salt of one element. Leaves of A. platanoides cultivated in Pb5 + Zn1, Pb1 + Zn1 and Pb1 + Zn5 experimental systems were characterised by specific browning of their edges. The obtained results suggest higher toxicity to leaves of Pb and Zn present simultaneously in Knop solution than Cu and Pb or Cu and Zn, irrespective of the mutual ratio of the concentrations of these elements. Antagonism of Cu and Zn concerning Pb was clearly shown in whole plant biomass when one of these elements was in higher concentration (5 mmol L-1) in solution. In the lowest concentrations (1 mmol L-1), there was a synergism between Cu and Zn in plant roots. Plants exposed to Zn5, Cu1 + Pb5, Pb5 + Zn1 and Cu1 + Zn1 were characterised by higher total phenolic content than the rest plants. Both the presence and the concentration of other elements in the soil are significant factors that modulate element uptake, total phenolic content, and plant development.

PMID:36378369 | DOI:10.1007/s11356-022-23966-x

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Nevin Manimala Statistics

Time series analysis of leishmaniasis incidence in Sri Lanka: evidence for humidity-associated fluctuations

Int J Biometeorol. 2022 Nov 15. doi: 10.1007/s00484-022-02404-0. Online ahead of print.

ABSTRACT

Leishmaniasis is a vector-borne disease of which the transmission is highly influenced by climatic factors, whereas the nature and magnitude differ between geographical regions. The effects of climatic variables on leishmaniasis in Sri Lanka are poorly investigated. The present study focused on time-series analysis of leishmaniasis cases reported from Sri Lanka with selected climatic variables. Variance stabilized time series of leishmaniasis patients of entire Sri Lanka and major districts from 2014 to 2018 was fitted to autoregressive integrated moving average (ARIMA) models. All the possible models were generated by assigning different values for autoregression and moving average terms using a function written in R statistical program. The top ten models with the lowest Akaike information criterion (AIC) values were selected by writing another function. These models were further evaluated using RMSE and MAPE error parameters to select the optimal model for each area. Cross-autocorrelation analyses were performed to assess the associations between climate and the leishmaniasis incidence. Most associated lags of each variable were integrated into the optimal models to determine the true effects imposed. The optimal models varied depending on the area. SARIMA (0,1,1) (1,0,0)12 was optimal for the country level. All the forecasts were within the 95% confidence intervals. Humidity was the most notable factor associated with leishmaniasis, which could be attributed to the positive impacts on sand fly activity. Rainfall showed a negative impact probably as a result of flooding of sand fly larval habitats. The ARIMA-based models performed well for the prediction of leishmaniasis in the short term.

PMID:36378349 | DOI:10.1007/s00484-022-02404-0

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Nevin Manimala Statistics

Gender Perspectives of Responses to Climate Variability and Change among Farm Households in Southeast Nigeria

Environ Manage. 2022 Nov 15. doi: 10.1007/s00267-022-01748-y. Online ahead of print.

ABSTRACT

It has been argued that gender affects climate change response especially among farming communities yet literature is scant on gender perspectives of response. In recognition of this, the study examined responses with the associated challenges and opportunities across gender in the South East (SE) Nigeria. A mixed research approach including a participatory research approach (PRA), field observation and household questionnaire survey was used to collect data. The survey sample included 300 (150 men and women each) farm household heads from six villages in the region. Data were analyzed and presented using principal component analysis (PCA), non-parametric and descriptive statistics. The study found that men and women-headed households differed in their use of strategies like migration from affected areas and livelihood diversification (p < 0.05). Women have more challenges of insufficient improved farming knowledge than men. The challenges for men were majorly insufficient technologies like mechanization. Components of these challenges include technology, environmental-related challenges among others. Response opportunities for men and women include digital/mobile telecommunications and learning by doing respectively. Compared to men, women had less participation in opportunities for the development of human capital through training (p < 0.05). The study recommends that more opportunities should be provided for the training of farmers especially women on climate change response.

PMID:36378319 | DOI:10.1007/s00267-022-01748-y

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Nevin Manimala Statistics

Minimum volume of infiltrative anesthetic required for pain-free placement of mini-implants: a split-mouth clinical trial

Quintessence Int. 2022 Nov 15;0(0):2-8. doi: 10.3290/j.qi.b3512065. Online ahead of print.

ABSTRACT

OBJECTIVE: To determine the minimum volume of infiltrative anesthetic required for pain-free mini-implant placement in the maxillary buccal region by comparing the efficacy of 1.0 mL with 0.5 mL of 2% lignocaine with 1:200,000 epinephrine during mini-implant placement.

METHOD AND MATERIALS: This splitmouth study involved 19 healthy patients without systemic disease, recent history of allergy, or medications within the age group of 17 to 28 years belonging to both sexes requiring bilateral buccal mini-implants in the posterior maxilla. Lignocaine 2% with 1:200,000 epinephrine (0.5 mL and 1.0 mL) was randomly injected between the right and left side 30 minutes apart for each consecutive patient. Mini-implants were placed 5 minutes after the administration of the infiltrative anesthetic. The pain response was evaluated during mini-implant placement (T1), and 5 minutes (T2) and 10 minutes (T3) after mini-implant placement on both sides using a pain-rating scale. Descriptive statistics and a factorial repeated-measure analysis of variance were calculated for pain response, sex, and side of the jaw.

RESULTS: At T1, T2, and T3, 1.0 mL of anesthetic had a lesser pain score by 1.00, 1.00, and 0.58, respectively, compared to 0.5 mL, with 95% confidence intervals of 0.43 to 1.57 (P = .001), 0.49 to 1.51 (P = .000), and 0.08 to 1.08 (P = .024), respectively.

CONCLUSIONS: 1.0 mL of 2% lignocaine with 1:200,000 epinephrine administered submucosally appears to provide better anesthesia than 0.5 mL during and after insertion of mini-implants. This study will help the operator administer the correct volume of infiltrative anesthetic thereby improving pain response, alleviating patient anxiety, and providing a better patient experience during and immediately after mini-implant placement.

PMID:36378301 | DOI:10.3290/j.qi.b3512065

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Influence of treatment setting on success of pulpectomy in primary molars: a retrospective analysis up to 4 years

Quintessence Int. 2022 Nov 15;0(0):2-11. doi: 10.3290/j.qi.b3512239. Online ahead of print.

ABSTRACT

OBJECTIVES: The objective of this study was to analyze the success of primary molar pulpectomy with a minimum of 1 year and up to 4 years follow-up with focus on the treatment setting (general anesthesia, sedation, local anesthesia alone).

METHOD AND MATERIALS: Data were retrieved from 92 patients’ records between 2012 and 2020. The pulpectomy treatment using calcium-hydroxide/iodoform paste was performed under general anesthesia (n = 45), nitrous oxide sedation (n = 21), or local anesthesia alone (n = 39). Bivariate and multivariate analyses were performed.

RESULTS: The overall success of pulpectomy was 59.5% 4 years post-treatment. The 4-years clinical success rate was clinically relevantly higher under general anesthesia (78.6% vs 57.1% under nitrous oxide sedation, 43.8% with local anesthesia only) and in the mandibular arch (70.8% vs 38.5% in the maxillary arch). This could be related to the strict case selection under sedation and especially general anesthesia. Despite statistically significant differences in the bivariate analysis for most outcomes and follow-up periods, this was not the case in multivariate regression.

CONCLUSION: Pulpectomy performed in primary molars offers a successful long-term treatment option especially with a strict case selection as under general anesthesia.

PMID:36378300 | DOI:10.3290/j.qi.b3512239

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Nevin Manimala Statistics

Impact of trough abiraterone level on adverse events in patients with prostate cancer treated with abiraterone acetate

Eur J Clin Pharmacol. 2022 Nov 15. doi: 10.1007/s00228-022-03420-0. Online ahead of print.

ABSTRACT

PURPOSE: We assessed the impact of plasma trough concentrations of abiraterone (ABI) and its metabolite Δ4-abiraterone (D4A) and related polymorphisms on adverse events (AEs) in patients with metastatic prostate cancer who received abiraterone acetate (AA).

METHODS: This prospective study enrolled patients with advanced prostate cancer treated with AA between 2016 and 2021. Plasma trough concentrations of ABI and D4A were measured using high-performance liquid chromatography. The impact of HSD3B1 rs1047303, SRD5A2 rs523349, and cytochrome P450 family 3A member 4 rs2242480 polymorphisms on plasma concentrations of ABI and D4A and the incidence of AEs were also assessed.

RESULTS: In 68 patients treated with AA, the median ABI and D4A concentrations were 18.1 and 0.94 ng/mL, respectively. The high plasma trough concentration of ABI (≥ 20.6 ng/mL) was significantly associated with the presence of any AE and its independent risk factor based on multivariable analysis (odds ratio, 7.20; 95% confidence interval (CI): 2.20-23.49). Additionally, a high plasma trough concentration of ABI was an independent risk factor of time to withdraw AA (hazard ratio, 4.89; 95% CI: 1.66-14.38). The risk alleles of three polymorphisms were not statistically associated with the ABI and D4A concentrations and the incidence of AEs.

CONCLUSIONS: The plasma trough concentration of ABI is associated with the presence of AEs and treatment failure after AA administration. ABI concentration monitoring may be useful in patients with prostate cancer who received AA.

PMID:36378297 | DOI:10.1007/s00228-022-03420-0