Category: Nevin Manimala

Braz J Microbiol. 2025 Sep 16. doi: 10.1007/s42770-025-01774-y. Online ahead of print.

ABSTRACT

Epstein-Barr virus (EBV) infection has been suggested as a potential cofactor for the development and progression of cervical cancer, collaborating with high-risk Human Papillomavirus (HR-HPV). In situ hybridization (ISH) has been considered the gold standard in the investigation of EBV in neoplasms. This study aimed to detect EBV in cervical carcinoma samples using ISH targeting EBERs (EBER-ISH) and the BamHI-W region of the viral genome (BamHI-W-ISH), and compare the results of both targets. Of the 88 cases collected, 9 were EBER-ISH positive (10.2%), while 33 (37.5%) cases were positive for EBV by BamHI-W-ISH, all showing staining in the nuclei of the malignant cells. No statistically significant results were found between the presence of EBV and carcinoma type, differentiation grade or tumor staging. The kappa agreement index between the two targets was 0.092. Only 4 cases were EBER-ISH(+) and BamHI-W-ISH(-). On the other hand, 28 cases were BamHI-W-ISH(+) and EBER-ISH(-). Altogether, 37/88 (42%) cases were EBV-positive by one or both targets. Infected lymphocytes were verified in 9 (10.2%) and 34 (38.6%) cases, by EBER-ISH and BamHI-W-ISH, respectively. The slight agreement demonstrated between the targets may be due to the lack of expression of EBERs, suggesting that EBV may present a distinct latency pattern in the cervical mucosa, or that it has entered the replicative cycle in some of these tumors, in both cases, explaining the low positivity rate verified through EBER-ISH, while calling into question the latter’s gold standard status in the detection of EBV in malignancies. Our findings also indicate that the chosen viral genomic target may represent a suitable candidate for EBV detection by ISH.

PMID:40956558 | DOI:10.1007/s42770-025-01774-y

Diabetes Ther. 2025 Sep 16. doi: 10.1007/s13300-025-01788-7. Online ahead of print.

ABSTRACT

INTRODUCTION: Early onset type 2 diabetes (T2D), diagnosed before age 40 years, is potentially more aggressive than later-onset disease and is increasing in prevalence globally. We examined the prevalence of early onset T2D in the USA and characterised this population.

METHODS: Data from the longitudinal series of NHANES cross-sectional surveys conducted between 1999 and 2020 were analysed retrospectively. The prevalence of diagnosed and undiagnosed early onset T2D was estimated across this period and the demographics, clinical characteristics and frequency of comorbidities in this population were described. Findings were compared with the US later-onset T2D population during the same period.

RESULTS: The prevalence of diagnosed and undiagnosed early onset T2D increased from 1.42% (standard error 0.19) and 0.18% (0.09), respectively, during the 1999-2000 survey cycle to 1.72% (0.24) and 0.35% (0.06), respectively, during the 2017-2020 cycle. Compared with those with later-onset disease, participants with early onset T2D had a lower mean poverty-income ratio, were more likely to be Hispanic or have no health insurance and less likely to be non-Hispanic white or have private or Medicare insurance (all p < 0.05). Individuals with early onset T2D generally had a worse cardiometabolic profile, with higher mean glycated haemoglobin, Homeostatic Model Assessment for Insulin Resistance score, fasting insulin and glucose, body mass index and waist circumference but were less likely to have congestive heart failure, coronary heart disease, stroke, chronic kidney disease or cancer (all p < 0.05). All comparisons remained statistically significant after adjustment for T2D duration among participants with diagnosed T2D.

CONCLUSIONS: These findings suggest that early onset T2D may disproportionately affect underserved populations with a higher likelihood of having cardiometabolic risk factors, suggesting a more aggressive disease that warrants the need for better diagnoses and treatment. Further research is needed to explore the potential link between cardiometabolic profile, risk of complications and longer-term cardiovascular outcomes in people with early onset T2D.

PMID:40956554 | DOI:10.1007/s13300-025-01788-7

Ann Surg Oncol. 2025 Sep 16. doi: 10.1245/s10434-025-18308-3. Online ahead of print.

ABSTRACT

BACKGROUND: En bloc resection of major pelvic sidewall neurovascular structures during pelvic exenteration is now deemed safe and oncologically feasible. This study aimed to evaluate the surgical, oncological, and quality-of-life (QoL) outcomes of patients who underwent en bloc common iliac or external iliac vein (CIV/EIV) resection during pelvic exenteration with and without venous reconstruction.

METHODS: This was a retrospective cohort study of patients who underwent en bloc CIV/EIV resection during pelvic exenteration at the Royal Prince Alfred Hospital, Sydney (January 1994-July 2024). The Functional Assessment of Cancer Therapy-Colorectal (FACT-C) questionnaire was used to evaluate QoL.

RESULTS: Of 76 patients included in this study, 41 underwent venous reconstruction and 35 did not (recon vs. non-recon groups). There were no statistically significant differences in surgical (including vascular-specific complications), oncological (R0), and QoL outcomes between groups. The non-recon group had increased major complications (42.9% vs. 36.6%) and length of hospital stay (28 vs. 22 days) but reduced estimated blood loss (3000 vs. 4500 mL). The non-recon group had superior median total FACT-C scores at 6- (102 vs. 100) and 12-month intervals (107 vs. 99.6).

CONCLUSION: Selected patients undergoing en bloc CIV/EIV excision without reconstruction may experience similar surgical, oncological, and QoL outcomes as those with reconstruction. When the CIV/EIV is resected, ligation without reconstruction can be performed safely in selected patients where there is evidence of chronic venous outflow obstruction and collateralization.

PMID:40956533 | DOI:10.1245/s10434-025-18308-3

Emerg Radiol. 2025 Sep 16. doi: 10.1007/s10140-025-02396-5. Online ahead of print.

ABSTRACT

PURPOSE: Traumatic adrenal gland hemorrhage (TAH) is an uncommon injury which can be managed conservatively in most cases. There are limited studies assessing the interval follow-up and resolution of conservatively managed TAH. The aim of our study was to evaluate the relationship between resolution of TAH, follow-up imaging interval and initial hematoma size. A key objective was to assess the incidence of underlying adrenal masses that may mimic or contribute to hemorrhage.

METHODS: Single centre retrospective cross-sectional study of all trauma patients with radiologically reported adrenal hemorrhage from January 1, 2009 to January 1, 2025. Patients were identified through radiology database search, with demographic, imaging and hematoma data collected to analyse associations between resolution, follow-up timing and initial hematoma size.

RESULTS: Of the 246 patients identified, 125 (51%) underwent at least 1 follow-up CT. The first follow-up occurred at a mean interval of 66.9 days; At this time, 60 patients (48%) showed complete resolution, 53 (42%) showed partial resolution and 12 (10%) demonstrated persistent hemorrhage. An underlying adrenal lesion was identified in 1% of patients. There were statistically significant associations between follow-up imaging time interval and hematoma resolution (p = 0.0025), and between the initial hematoma size and the resolution outcome (p < 0.000001).

CONCLUSION: Complete resolution of TAH occurred more frequently in patients with follow-up imaging at ≥ 30 days post injury. Hematomas measuring < 27 mm on initial imaging were more likely to resolve completely. Underlying adrenal lesions were rare. These findings may assist trauma centres in refining follow-up imaging strategies for conservatively managed TAH.

PMID:40956525 | DOI:10.1007/s10140-025-02396-5

Genes Genomics. 2025 Sep 16. doi: 10.1007/s13258-025-01673-4. Online ahead of print.

ABSTRACT

BACKGROUND: Identifying microbiome markers associated with ordered phenotypes, such as disease stages or severity levels, is crucial for understanding disease progression and advancing precision medicine. Despite this importance, most existing methods for differential abundance analysis are designed for binary group comparisons and do not incorporate ordinal information, limiting their ability to capture trends across ordered categories.

OBJECTIVE: To develop and evaluate statistical methods that explicitly account for ordinal phenotype structure in microbiome data, addressing challenges such as sparsity and zero inflation, and improving the detection of meaningful microbial associations.

METHODS: In this study, we propose and evaluate three novel approaches specifically tailored for microbiome association analysis with ordered groups: the binary optimal test, the linear trend test, and the proportional odds model-based permutation test (POMp). These methods explicitly account for the ordinal structure of phenotypes and address the sparsity and zero-inflation commonly observed in microbiome data through permutation-based inference. We applied the proposed methods to three publicly available gut microbiome datasets, including two related to obesity and one concerning colorectal cancer.

RESULTS: All three proposed methods successfully identified differentially abundant features (DAFs) that exhibited stronger ordinal associations compared to those identified by existing methods. In particular, POMp consistently outperformed other approaches in terms of correlation with phenotype order, demonstrating its potential to identify biologically relevant markers.

CONCLUSION: The findings of this study highlight the importance of incorporating ordinal information in microbiome studies and provide robust statistical tools for advancing microbial biomarker discovery in complex disease contexts.

PMID:40956524 | DOI:10.1007/s13258-025-01673-4

Clin Drug Investig. 2025 Sep 16. doi: 10.1007/s40261-025-01482-3. Online ahead of print.

ABSTRACT

BACKGROUND AND OBJECTIVES: Dyslipidaemia is a key modifiable risk factor for atherosclerotic cardiovascular disease. However, achieving recommended low-density lipoprotein cholesterol (LDL-C) target levels is challenging owing to dose-dependent adverse effects and limited tolerability of high-dose statins. This study evaluated the real-world efficacy and safety of combining very-low-dose rosuvastatin (2.5 mg) with ezetimibe (10 mg) in adult patients with dyslipidaemia across different cardiovascular risk strata.

METHODS: This multicentre prospective study in South Korea enrolled 2,388 patients. Participants were stratified into low-, moderate-, or high-risk groups on the basis of the 2019 European Society of Cardiology and European Atherosclerosis Society guidelines. Lipid profiles and safety outcomes were assessed at baseline and after 12 weeks. The primary and secondary outcomes were LDL-C and non-high-density lipoprotein cholesterol (non-HDL-C) target level achievements, respectively, and adverse events were monitored.

RESULTS: After 12 weeks, LDL-C target levels were achieved by 82.6% of low-risk (< 116 mg/dL), 73.9% of moderate-risk (< 100 mg/dL), and 50.4% of high-risk (< 70 mg/dL) patients. Non-HDL-C target level achievement followed a similar trend. Combination therapy with ezetimibe and low-dose statin resulted in significant LDL-C reductions, compared with statins alone. Adverse events were infrequent (0.6%), and only 0.2% of patients discontinued treatment owing to medication-related concerns.

CONCLUSIONS: Very-low-dose rosuvastatin-ezetimibe combination therapy significantly lowered LDL-C levels and improved lipid profiles across various risk groups, demonstrating a favourable safety profile. These findings support its use as an effective, well-tolerated option for managing dyslipidaemia. Longer-term studies are warranted to evaluate sustained lipid control and cardiovascular outcomes.

PMID:40956520 | DOI:10.1007/s40261-025-01482-3

Hormones (Athens). 2025 Sep 16. doi: 10.1007/s42000-025-00718-3. Online ahead of print.

ABSTRACT

BACKGROUND: Chronic inflammation plays a pivotal role in autoimmune thyroiditis (AT), yet traditional biomarkers fail to predict systemic complications. The systemic immune-inflammation Index (SII) and the systemic inflammation response index (SIRI), which integrate multiple immune cell counts, may serve as novel prognostic tools for assessing AT-related mortality.

METHODS: This study analyzed data from 1053 AT patients in the 2007-2012 NHANES cycles. SII and SIRI were calculated using standardized complete blood count parameters. All-cause mortality was assessed through linkage with the National Death Index. Cox proportional hazard models with sequential adjustments evaluated the associations between inflammatory indices and mortality. Nonlinear relationships and critical thresholds were examined using restricted cubic splines.

RESULTS: Elevated SII and SIRI were significantly associated with increased mortality risk. After full adjustment, each log-unit increase in SII (HR = 1.819, 95% CI:1.347-2.457) and each unit increase in SIRI (HR = 1.314, 95% CI:1.124-1.537) independently predicted higher mortality. Threshold analysis identified critical inflection points at ln-SII ≥ 6.18 (HR = 2.629, 95% CI:1.431-4.831) and SIRI ≥ 1.01 (HR = 1.257, 95% CI:1.030-1.535), beyond which mortality risk escalated sharply. Kaplan-Meier curves confirmed significant survival disparities across tertiles (log-rank p < 0.001). Stratified analyses showed consistent associations across demographic and clinical subgroups.

CONCLUSION: SII and SIRI are robust, cost-effective biomarkers for predicting mortality in AT, with defined thresholds marking transitions from compensatory to pathological inflammation. These indices provide a comprehensive reflection of systemic immune dysregulation, offering actionable insights for risk stratification and targeted interventions. Future studies should validate these findings in diverse populations and explore anti-inflammatory therapies to improve outcomes in AT patients.

PMID:40956512 | DOI:10.1007/s42000-025-00718-3

Odontology. 2025 Sep 16. doi: 10.1007/s10266-025-01200-7. Online ahead of print.

ABSTRACT

This in vitro study aimed to evaluate the changes in surface integrity of dental enamel after bleaching using gels with different compositions followed by an erosive challenge. Bovine enamel specimens (4 × 4 × 2 mm; n = 12/group) stored in artificial saliva were treated with bleaching gels containing carbamide peroxide (CP) or hydrogen peroxide (HP) with or without calcium polyphosphate (CaPP) followed by an erosive challenge. In Experiment 1, 10% commercial CP (CPC), CPC + 2% NaF, 10% manipulated CP (CPM), CPM + 2% NaF, 10% CP + 0.5 wt% CaPP, 10% CP + 1.5 wt% CaPP, manipulated gel without CP, or no bleaching were applied for 14 days. In Experiment 2, CP was switched with 35% HP, and all gels were applied for two sessions. Thereafter, all samples were subjected to a 5-day erosive challenge using 1% citric acid (pH 3.5) for 120 s. Enamel surface loss was assessed using contact profilometry and scanning electron microscopy (SEM). Statistical analysis was performed using a generalized linear model (α = 0.05). Compared with that in the CP + 0.5% CaPP and HP + 0.5% CaPP groups, enamel loss was significantly greater in the CPM and CPC groups and HPM and HPC groups in Experiments 1 and 2, respectively. SEM analysis revealed less surface degradation in the groups containing 0.5% CaPP, with greater preservation of the prismatic and interprismatic enamel structure. Both low- and high-concentration bleaching gels containing 0.5% CaPP improved enamel preservation after an erosive challenge compared to that with gels without CaPP. Thus, the addition of CaPP may help minimize enamel damage during bleaching by reducing mineral loss.

PMID:40956501 | DOI:10.1007/s10266-025-01200-7

Ir J Med Sci. 2025 Sep 16. doi: 10.1007/s11845-025-04076-2. Online ahead of print.

ABSTRACT

BACKGROUND: Growing patient interest in the direct anterior approach (DAA) has led to increased Internet promotion by surgeons.

AIMS: The aim of this study was to investigate Internet promotion of the DAA by surgeons in Australia, New Zealand, Ireland and the UK. Further, we aimed to determine if surgeons offering the DAA discussed the potential benefits of the approach more frequently than other surgeons.

METHODS: Orthopaedic surgeons were identified from the websites of the Australian Orthopaedic Association, New Zealand Orthopaedic Association, UK National Joint Registry, and Irish Institute of Trauma and Orthopaedic Surgery. Each surgeon website was investigated for claims made regarding the risks and benefits of the DAA, as well as the literature used to support these claims.

RESULTS: In total, 6.4% (n = 177) of websites identified mentioned the DAA: 128 (72.3%) websites were associated with surgeons offering the DAA, while 49 (27.7%) websites were associated with surgeons not offering the DAA. Potential benefits were mentioned in 74% of websites, while 36.7% made reference to risks; 9% cited peer-reviewed literature. When comparing websites of surgeons who offer the DAA with the websites of surgeons who do not, there was a statistically significantly increased rate of mentioning any benefit (p = 0.016), less tissue damage (p = 0.041), less blood loss (p = 0.004), faster recovery (p = 0.004), and fewer dislocations (p = 0.002).

CONCLUSION: A small number of surgeons in these countries promote the DAA online. Surgeons offering the DAA are more likely to report the potential benefits of the procedure than surgeons not offering the DAA.

PMID:40956496 | DOI:10.1007/s11845-025-04076-2

Eur J Pediatr. 2025 Sep 16;184(10):619. doi: 10.1007/s00431-025-06470-5.

ABSTRACT

OBJECTIVES: This study aimed to assess the impact of malnutrition on liver and spleen stiffness in children using shear wave elastography (SWE) and to compare the findings with those of healthy controls.

METHODS: A total of 308 children aged 0-18 years were prospectively enrolled and classified as either malnourished or healthy according to World Health Organization (WHO) growth reference standards (weight-for-height Z-scores for children under 5 years and BMI-for-age Z-scores for those aged 5 years and older). Liver and spleen stiffness measurements were obtained using SWE by a single pediatric radiologist blinded to nutritional status. Statistical comparisons were performed between groups, across malnutrition severity subgroups, and within different age categories.

RESULTS: Liver stiffness did not differ significantly between malnourished children overall and healthy controls, but it was markedly higher in those with severe malnutrition compared to mild or moderate cases (p = 0.018). Spleen stiffness was significantly lower in malnourished children aged 0-6 years compared to controls (p < 0.001). Both liver and spleen stiffness values increased with age (p = 0.014 and p = 0.013, respectively).

CONCLUSION: Our findings indicate that malnutrition is associated with age- and severity-dependent alterations in liver and spleen stiffness. Liver stiffness was elevated only in children with severe malnutrition, whereas spleen stiffness was reduced in younger children but not in older ones. SWE may serve as a valuable non-invasive tool for assessing organ-level effects of malnutrition, and these results may guide future research on the reversibility and clinical significance of such changes.

WHAT IS KNOWN: • Childhood malnutrition is associated with profound metabolic and structural alterations in vital organs such as the liver and spleen. However, its impact on organ biomechanics, as assessed by elastography, has not been clearly defined.

WHAT IS NEW: • Malnutrition leads to organ-specific biomechanical changes, with increased liver stiffness in severe cases and reduced spleen stiffness particularly in younger children. • Shear wave elastography provides a non-invasive approach to characterize these alterations.

PMID:40956488 | DOI:10.1007/s00431-025-06470-5