Category: Nevin Manimala

Bone Marrow Transplant. 2023 Nov 25. doi: 10.1038/s41409-023-02147-5. Online ahead of print.

ABSTRACT

Allogeneic haematopoietic cell transplantation (alloHCT) has curative potential counterbalanced by its toxicity. Prognostic scores fail to include current era patients and alternative donors. We examined adult patients from the EBMT registry who underwent alloHCT between 2010 and 2019 for oncohaematological disease. Our primary objective was to develop a new prognostic score for overall mortality (OM), with a secondary objective of predicting non-relapse mortality (NRM) using the OM score. AI techniques were employed. The model for OM was trained, optimized, and validated using 70%, 15%, and 15% of the data set, respectively. The top models, “gradient boosting” for OM (AUC = 0.64) and “elasticnet” for NRM (AUC = 0.62), were selected. The analysis included 33,927 patients. In the final prognostic model, patients with the lowest score had a 2-year OM and NRM of 18 and 13%, respectively, while those with the highest score had a 2-year OM and NRM of 82 and 93%, respectively. The results were consistent in the subset of the haploidentical cohort (n = 4386). Our score effectively stratifies the risk of OM and NRM in the current era but do not significantly improve mortality prediction. Future prognostic scores can benefit from identifying biological or dynamic markers post alloHCT.

PMID:38007531 | DOI:10.1038/s41409-023-02147-5

Fa Yi Xue Za Zhi. 2023 Oct 25;39(5):447-451. doi: 10.12116/j.issn.1004-5619.2021.511207.

ABSTRACT

OBJECTIVES: To establish the menstrual blood identification model based on Naïve Bayes and multivariate logistic regression methods by using specific mRNA markers in menstrual blood detection technology combined with statistical methods, and to quantitatively distinguish menstrual blood from other body fluids.

METHODS: Body fluids including 86 menstrual blood, 48 peripheral blood, 48 vaginal secretions, 24 semen and 24 saliva samples were collected. RNA of the samples was extracted and cDNA was obtained by reverse transcription. Five menstrual blood-specific markers including members of the matrix metalloproteinase (MMP) family MMP3, MMP7, MMP11, progestogens associated endometrial protein (PAEP) and stanniocalcin-1 (STC1) were amplified and analyzed by electrophoresis. The results were analyzed by Naïve Bayes and multivariate logistic regression.

RESULTS: The accuracy of the classification model constructed was 88.37% by Naïve Bayes and 91.86% by multivariate logistic regression. In non-menstrual blood samples, the distinguishing accuracy of peripheral blood, saliva and semen was generally higher than 90%, while the distinguishing accuracy of vaginal secretions was lower, which were 16.67% and 33.33%, respectively.

CONCLUSIONS: The mRNA detection technology combined with statistical methods can be used to establish a classification and discrimination model for menstrual blood, which can distignuish the menstrual blood and other body fluids, and quantitative description of analysis results, which has a certain application value in body fluid stain identification.

PMID:38006263 | DOI:10.12116/j.issn.1004-5619.2021.511207

J Hum Lact. 2023 Nov 25:8903344231210813. doi: 10.1177/08903344231210813. Online ahead of print.

ABSTRACT

BACKGROUND: Breastfeeding is very important for maternal and infant health. With first pregnancies, many pregnant people face obstacles to achieving their breastfeeding goals.

RESEARCH AIMS: We aimed to investigate the outcomes of a breastfeeding education program and nurse-led online breastfeeding counseling system (BMUM) on breastfeeding self-efficacy, attitudes about breastfeeding, breastfeeding problems, breastfeeding frequencies and postpartum depression.

METHODS: This study was a randomized controlled trial. Participants were randomly assigned to the intervention group (n = 36), or control group (n = 36). Assessments were conducted during pregnancy, between 32- and 37-weeks gestation, and on postpartum Day 1, Week 1, Week 3, and 6 months.

RESULTS: The means of the Breastfeeding Self-Efficacy-Short Form scores, and the Infant Feeding Attitude Scale (IIFAS) scores were similar between the groups at the first assessment (p = 0.733). IIFAS scores in the intervention group were significantly higher in the follow-up measurements on postpartum Day 1, Week 1, Week 3, and 6 months compared to scores in the control group (p = 0.006; p = 0.000; p = 0.002; p = 0.001) Edinburgh Postpartum Depression Scale (EPDS) scores were similar between the two groups at 1 week (p = 0.678). EPDS scores were significantly higher in the control group on Day 1 and at 3 and 6 months postpartum (p = 0.000; p = 0.038; p = 0.042). There was no statistically significant difference in breastfeeding problems between the two groups (p > 0.05 across breastfeeding problems examined). The mean values of breastfeeding frequency were similar between groups on Day 1, and significantly higher in the intervention group on follow-up measurements.

CONCLUSION: The results of this intervention appear to promote positive attitudes toward breastfeeding and decrease feelings of postpartum depression. However, further randomized controlled trials are needed to support our outcomes.

PMID:38006250 | DOI:10.1177/08903344231210813

Hum Reprod. 2023 Nov 24:dead244. doi: 10.1093/humrep/dead244. Online ahead of print.

ABSTRACT

STUDY QUESTION: What is the impact of clinically significant weight change on outcomes related to IVF cycle performance?

SUMMARY ANSWER: While individual weight loss did not significantly impact ovarian response to stimulation or other cycle outcome parameters in our study, some positive associations were found for individual weight gain.

WHAT IS KNOWN ALREADY: The role of weight-change in patients undergoing IVF has been largely studied by comparing weight loss in different cohorts of patients stratified by a static BMI. Specifically, obesity has been extensively studied in relation to its negative effects on assisted or unassisted conception outcomes and ovulatory function. Previous research has shown conflicting results, while BMI, which is commonly used as a marker of obesity, may not accurately reflect the underlying factors affecting fertility in obese patients.

STUDY DESIGN, SIZE, DURATION: This study utilized a retrospective within-patient repeated measurement analysis design to assess the impact of weight change on IVF outcomes in cycles where all embryos were cryopreserved at the blastocyst stage for transfer at a later date.

PARTICIPANTS/MATERIALS, SETTING, METHODS: The study was conducted at an academically affiliated fertility center. The data included 961 women who underwent at least two IVF cycles between December 2014 and June 2020, with documented short-term weight gain (n = 607) or weight loss (n = 354) within 1 year from their initial IVF cycle. Multivariable generalized estimating equations (GEE) and generalized linear mixed models (GLMM) were employed to assess associations between weight change and outcomes across cycles.

MAIN RESULTS AND THE ROLE OF CHANCE: The multivariable models indicated that weight loss did not show any significant associations with the numbers of oocytes retrieved, or mature oocytes, the fertilization rate or the blastulation rate. However, weight gain demonstrated a minor positive association with the number of oocytes retrieved in both GEE models (coefficient: 0.01, 95% CI: 0.00-0.01) and GLMM models (0.01, 95% CI: 0.01-0.00). There was also a potential increase in the fertilization rate with weight gain, as indicated by a positive coefficient in both GEE models (coefficient: 0.01, 95% CI: 0.00-0.02) and GLMM models (coefficient: 0.01, 95% CI: 0.00-0.01). However, the association between weight gain and the embryo blastulation rate was not statistically significant in any model.

LIMITATIONS, REASONS FOR CAUTION: This study focused on cycle performance parameters instead of reproductive outcomes, which restricted our ability to evaluate the impact of weight change on cumulative live birth rates. Additionally, the study did not account for variables such as stimulation protocols, potentially introducing confounding factors and limiting the generalizability of the results.

WIDER IMPLICATIONS OF THE FINDINGS: Although obesity is associated with adverse obstetrical risks, there is less evidence of adverse reproductive outcomes in IVF cycles. We therefore recommend that an IVF cycle should not be delayed due to weight, so that the patient is not adversely affected by increasing age. The IVF cycle should aim to freeze all embryos, so that embryo transfer can then occur after weight loss, so as to limit the recognized obstetrical risks.

STUDY FUNDING/COMPETING INTEREST(S): The study was not funded and there were no competing interests.

TRIAL REGISTRATION NUMBER: N/A.

PMID:38006233 | DOI:10.1093/humrep/dead244

Nucleosides Nucleotides Nucleic Acids. 2023 Nov 24:1-10. doi: 10.1080/15257770.2023.2282572. Online ahead of print.

ABSTRACT

Major depressive disorder (MDD), which is a prevalent psychiatric disorder, is characterized by sleep-wake disturbances. An underlying circadian rhythm disorder mainly may cause these disturbances. The study presented here was designed to investigate the existence of Period Circadian Regulator 3 (PER3) gene VNTR variant in MDD patients in Turkish population. A sample of 118 patients with MDD and 150 healthy volunteers were included in the study. The PER3 VNTR genotyping was performed on DNA by polymerase chain reaction (PCR) using specific primers. The prevalence rates of genotypes of 5/5, 5/4, and 4/4 profiles for the PER3 variant were 30.5%, 55.9%, and 13.6%, respectively, in patients with MDD, and 23.3%, 57.3%, and 19.3%, respectively in the control group. No significant difference was observed between the two groups in terms of either genotype distributions or allele frequencies of the VNTR variant of the PER3 gene (p > 0.05). There was no statistically significant association between the patients and the controls in terms of 5/5 + 4/5 versus 4/4 and 5/5 versus 4/5 + 4/4 (p > 0.05). The present results suggest that the PER3 VNTR variant was not associated with MDD in the Turkish population. However, further studies with other gene variants in different ethnic populations are needed to address the exact role of this variant in MDD.

PMID:38006223 | DOI:10.1080/15257770.2023.2282572

HGG Adv. 2023 Nov 23:100258. doi: 10.1016/j.xhgg.2023.100258. Online ahead of print.

ABSTRACT

Ebstein’s anomaly, a rare congenital heart disease, is distinguished by the failure of embryological delamination of the tricuspid valve leaflets from the underlying primitive right ventricle myocardium. Gaining insight into the genetic basis of Ebstein’s anomaly allows for a more precise definition of its pathogenesis. In this study, two distinct cohorts from the Chinese Han population were included: a case-control cohort consisting of 82 unrelated cases and 125 controls without cardiac phenotypes, and a trio cohort comprising 36 parent-offspring trios. Whole-exome sequencing data from all 315 participants were utilized to identify qualifying variants, encompassing rare (minor allele frequency < 0.1% from East Asians in gnomAD database) functional variants and high-confidence (HC) loss-of-function (LoF) variants. Various statistical models, including burden tests and variance-component models, were employed to identify rare variants, genes, and biological pathways associated with Ebstein’s anomaly. Significant associations were noted between Ebstein’s anomaly and rare HC LoF variants found in genes related to the matrisome, a collection of extracellular matrix (ECM) components. Specifically, 47 genes with HC LoF variants were exclusively or predominantly identified in cases, while nine genes showed such variants in the probands. Over half of unrelated cases (n=42) and approximately one-third of probands (n=12) were found to carry one or two LoF variants in these prioritized genes. These results highlight the role for the matrisome in the pathogenesis of Ebstein’s anomaly, contributing to a better understanding of the genetic architecture underlying this condition. Our findings hold the potential to impact the genetic diagnosis and treatment approaches for Ebstein’s anomaly.

PMID:38006208 | DOI:10.1016/j.xhgg.2023.100258

Polymers (Basel). 2023 Nov 20;15(22):4469. doi: 10.3390/polym15224469.

ABSTRACT

As a light-weight solution for electromagnetic shielding, this paper aims to investigate the development of electrically conductive composites that shield from electromagnetic radiation while providing sustainability by using recycled fibers in the structure of nonwoven reinforcement materials. The main novelty of this research is the conversion of waste fabrics into functional composites via a fast and inexpensive method. For this purpose, waste fabrics were recycled into fibers, and the recycled fibers were processed into needle-punched nonwovens to be used as reinforcement materials for electromagnetic shielding composites. Electrically conductive composite structures were obtained by adding copper (II) sulfate and graphite conductive particles with different ratios to polyester resin. The hand lay-up method was used for the production of composites. Electromagnetic shielding, electrical resistivity, and some mechanical properties of the composites were investigated. The results were analyzed statistically using IBM SPSS software version 18. The results have shown that up to 31.43 dB of electromagnetic shielding effectiveness was obtained in the 1-6 GHz frequency range. This result corresponds to a very good grade for general use and a moderate grade for professional use, according to FTTS-FA-003, exceeding the acceptable range for industrial and commercial applications of 20 dB. The composites developed in this research are good candidates to be used in various general and professional applications, such as plastic parts in household applications, electronic industry, building and construction industries, and other applications where light weight shielding materials are needed.

PMID:38006193 | DOI:10.3390/polym15224469

Polymers (Basel). 2023 Nov 16;15(22):4434. doi: 10.3390/polym15224434.

ABSTRACT

Polyurethane and polyurea-based adhesives are widely used in various applications, from automotive to electronics and medical applications. The adhesive performance depends strongly on its composition, and developing the formulation-structure-property relationship is crucial to making better products. Here, we investigate the dependence of the linear viscoelastic properties of polyurea nanocomposites, with an IPDI-based polyurea (PUa) matrix and exfoliated graphene nanoplatelet (xGnP) fillers, on the hard-segment weight fraction (HSWF) and the xGnP loading. We characterize the material using scanning electron microscopy (SEM) and dynamic mechanical analysis (DMA). It is found that changing the HSWF leads to a significant variation in the stiffness of the material, from about 10 MPa for 20% HSWF to about 100 MPa for 30% HSWF and about 250 MPa for the 40% HSWF polymer (as measured by the tensile storage modulus at room temperature). The effect of the xGNP loading was significantly more limited and was generally within experimental error, except for the 20% HSWF material, where the xGNP addition led to about an 80% increase in stiffness. To correctly interpret the DMA results, we developed a new physics-based rheological model for the description of the storage and loss moduli. The model is based on the fractional calculus approach and successfully describes the material rheology in a broad range of temperatures (-70 °C-+70 °C) and frequencies (0.1-100 s^-1), using only six physically meaningful fitting parameters for each material. The results provide guidance for the development of nanocomposite PUa-based materials.

PMID:38006160 | DOI:10.3390/polym15224434

Polymers (Basel). 2023 Nov 16;15(22):4417. doi: 10.3390/polym15224417.

ABSTRACT

This study aimed to extract and characterize the morphological, physicochemical, thermal, and rheological properties of the starches of native potatoes grown in the department of Puno. Among the varieties evaluated were sweet native potato varieties Imilla Negra (Solanum tuberosum spp. Andígena), Imilla Blanca (Solanum tuberosum spp. Andígena), Peruanita, Albina or Lomo (Solanum chaucha), and Sutamari, and the bitter potatoes Rucki or Luki (Solanum juzepczukii Buk), Locka (Solanum curtilobum), Piñaza (Solanum curtilobum), and Ocucuri (Sola-num curtilobum), acquired from the National Institute of Agrarian Innovation (INIA-Puno). The proximal composition, amylose content, and morphological, thermal, and rheological properties that SEM, DSC, and a rheometer determined, respectively, were evaluated, and the data obtained were statistically analyzed using a completely randomized design and then a comparison of means using Tukey’s LSD test. The results show a significant difference in the proximal composition (p ≤ 0.05) concerning moisture content, proteins, fat, ash, and carbohydrates. Thus, the amylose content was also determined, ranging from 23.60 ± 0.10 to 30.33 ± 0.15%. The size morphology of the granules is 13.09-47.73 µm; for the thermal and rheological properties of the different varieties of potato starch, it is shown that the gelatinization temperature is in a range of 57 to 62 °C and, for enthalpy, between 3 and 5 J/g.

PMID:38006141 | DOI:10.3390/polym15224417

Polymers (Basel). 2023 Nov 8;15(22):4350. doi: 10.3390/polym15224350.

ABSTRACT

In an interlayered carbon fiber-reinforced polycarbonate polymer (CFRPC) composite composed of three sized of CF plies, alternating between four PC sheets, designated [PC]₄[CF]₃, and a new process of activating CF cross-weave cloth plies directly on both sides with homogeneous low-energy electron beam irradiation (HLEBI) before lamination assembly and hot pressing at 6.0 MPa and 537 K for 8 min was produced. Experimental results show that a dose of 215 kGy of HLEBI raised the bending strength, σ_b, at each experimental accumulative probability, P_a, with the σ_b at a median P_a of 0.50, increasing by 25% over that of the untreated sample. Three-parameter Weibull analysis showed that when quality can be controlled, a dose of 215 kGy of HLEBI can raise the statistically lowest bending strength, σ_s, at P_a = 0 (94.3 Mpa), with a high correlation coefficient. This is because, although it had a higher bending strength than that in the other experimental conditions, the weakest sample of the 215 kGy data set had a much lower σ_b value than that of the others. Electron spin resonance (ESR) of the CF showed that naturally occurring dangling bonds in CF were increased at 215 kGy. Charge transfer to the PC occurs, apparently generating stronger bonds, which are possibly covalent, resulting in enhanced adhesion at the CF-PC interface.

PMID:38006075 | DOI:10.3390/polym15224350