Category: Nevin Manimala

PLoS One. 2023 May 10;18(5):e0285452. doi: 10.1371/journal.pone.0285452. eCollection 2023.

ABSTRACT

Italy was the first European country to be significantly impacted by the COVID-19 pandemic. The lack of similar previous experiences and the initial uncertainty regarding the new virus resulted in an unpredictable health crisis with 243,506 total confirmed cases and 34,997 deaths between February and July 2020. Despite the panorama of precariousness and the impelling calamity, the country successfully managed many aspects of the early stages of the health and socio-economic crisis. Nevertheless, many disparities can be identified at the regional level. The study aims to determine which aspects of regional management were considered more important by the citizens regarding economic and health criteria. A survey was designed to gather responses from the population on the Italian regions’ response and provide a ranking of the regions. The 29-item online survey was provided to 352 individuals, and the collected data were analyzed using the Analytic Hierarchy Process methodology. The results show a general agreement in considering of greater relevance the healthcare policies rather than the economic countermeasures adopted by regional governments. Our analysis associated a weight of 64% to the healthcare criteria compared to the economic criteria with a weight of 36%. In addition to the results obtained from the Analytic Hierarchy Process, the sample’s composition was analyzed to provide an overall assessment of the Italian regions. To do so, we collected objective data for each region and multiplied them by the overall weight obtained for each sub-criteria. Looking at the propensity to vaccination or the belief in a relation between COVID-19 and 5G according to age and educational qualification helps understand how public opinion is structured according to cultural and anthropological differences.

PMID:37163510 | DOI:10.1371/journal.pone.0285452

PLoS One. 2023 May 10;18(5):e0284141. doi: 10.1371/journal.pone.0284141. eCollection 2023.

ABSTRACT

BACKGROUND: Breast and cervical cancers are in the top 10 most common cancers in women globally and the most common cancers in Nigerian women. The incidences have been rising steadily over the years. Involvement of men as key players in reproductive health issues has been receiving global attention especially in low and middle-income countries.

AIM: To assess male involvement in their female partners’ screening for breast and cervical cancers in Southwest, Nigeria.

METHOD: This was a community-based, cross-sectional study that employed a multi-stage sampling method to select 254 men who were married or in steady relationships in Lagos State, Southwest Nigeria. Data were collected from June to October 2018 using a semi-structured interviewer-administered questionnaire, analyzed using Epi Info version 3.5.1 and summarized with mean and standard deviation. Chi-square test was used for bivariate statistics, and the p-value of ≤0.05 was considered statistically significant. Multivariable logistic regression was used for predictor variables of male involvement in screening.

RESULTS: 29.5% of the respondents had good knowledge of breast and cervical cancers and screening and majority (85.5%) had a positive attitude towards screening. Only few, 19.3% and 15.7% had provided money for breast and cervical cancer screening respectively. Most men, 75% and 87.4% respectively had not accompanied their wife/female partner for breast and cervical cancer screening, while almost half (49.2%) and one-third (33.5%) respectively, had encouraged their female partners to screen for breast and cervical cancers. Overall, only about half, 138 (54.3%) of the men were considered ‘involved’ in their female partners’ screening for breast and cervical cancers. Male involvement was significantly associated with screening for female cancers (χ2 = 77.62, p = 0.001). Older age group (AOR = 2.64, 95% CI: 1.3-4.9), higher educational attainment (AOR = 3.51, 95% CI: 1.14-10.73), and positive attitude (AOR = 2.48, 95% CI:1.16-5.33) were found to be the predictors of male involvement.

CONCLUSION: Community-based programs for males, especially the younger and less educated, should be implemented to increase their involvement. It is also suggested that mass media messages be spread and online platforms be explored in order to increase men’s awareness and participation in female cancer screening.

PMID:37163507 | DOI:10.1371/journal.pone.0284141

PLoS One. 2023 May 10;18(5):e0283353. doi: 10.1371/journal.pone.0283353. eCollection 2023.

ABSTRACT

In 2015, the Open Science Collaboration repeated a series of 100 psychological experiments. Since a considerable part of these replications could not confirm the original effects and some of them pointed in the opposite direction, psychological research is said to lack reproducibility. Several general criticisms can explain this finding, such as the standardized use of undirected nil-null hypothesis tests, samples being too small and selective, lack of corrections for multiple testing, but also some widespread questionable research practices and incentives to publish positive results only. A selection of 57,909 articles from 12 renowned journals is processed with the JATSdecoder software to analyze the extent to which several empirical research practices in psychology have changed over the past 12 years. To identify journal- and time-specific changes, the relative use of statistics based on p-values, the number of reported p-values per paper, the relative use of confidence intervals, directed tests, power analysis, Bayesian procedures, non-standard α levels, correction procedures for multiple testing, and median sample sizes are analyzed for articles published between 2010 and 2015 and after 2015, and in more detail for every included journal and year of publication. In addition, the origin of authorships is analyzed over time. Compared to articles that were published in and before 2015, the median number of reported p-values per article has decreased from 14 to 12, whereas the median proportion of significant p-values per article remained constant at 69%. While reports of effect sizes and confidence intervals have increased, the α level is usually set to the default value of .05. The use of corrections for multiple testing has decreased. Although uncommon in each case (4% in total), directed testing is used less frequently, while Bayesian inference has become more common after 2015. The overall median estimated sample size has increased from 105 to 190.

PMID:37163505 | DOI:10.1371/journal.pone.0283353

PLoS One. 2023 May 10;18(5):e0282924. doi: 10.1371/journal.pone.0282924. eCollection 2023.

ABSTRACT

Recent years have seen a substantial growth in the adoption of machine learning approaches for the purposes of quantitative structure-activity relationship (QSAR) development. Such a trend has coincided with desire to see a shifting in the focus of methodology employed within chemical safety assessment: away from traditional reliance upon animal-intensive in vivo protocols, and towards increased application of in silico (or computational) predictive toxicology. With QSAR central amongst techniques applied in this area, the emergence of algorithms trained through machine learning with the objective of toxicity estimation has, quite naturally, arisen. On account of the pattern-recognition capabilities of the underlying methods, the statistical power of the ensuing models is potentially considerable-appropriate for the handling even of vast, heterogeneous datasets. However, such potency comes at a price: this manifesting as the general practical deficits observed with respect to the reproducibility, interpretability and generalisability of the resulting tools. Unsurprisingly, these elements have served to hinder broader uptake (most notably within a regulatory setting). Areas of uncertainty liable to accompany (and hence detract from applicability of) toxicological QSAR have previously been highlighted, accompanied by the forwarding of suggestions for “best practice” aimed at mitigation of their influence. However, the scope of such exercises has remained limited to “classical” QSAR-that conducted through use of linear regression and related techniques, with the adoption of comparatively few features or descriptors. Accordingly, the intention of this study has been to extend the remit of best practice guidance, so as to address concerns specific to employment of machine learning within the field. In doing so, the impact of strategies aimed at enhancing the transparency (feature importance, feature reduction), generalisability (cross-validation) and predictive power (hyperparameter optimisation) of algorithms, trained upon real toxicity data through six common learning approaches, is evaluated.

PMID:37163504 | DOI:10.1371/journal.pone.0282924

PLoS One. 2023 May 10;18(5):e0285277. doi: 10.1371/journal.pone.0285277. eCollection 2023.

ABSTRACT

By using a Gaussian process prior and a location-scale mixture representation of the asymmetric Laplace distribution, we develop a Bayesian analysis for the composite quantile single-index regression model. The posterior distributions for the unknown parameters are derived, and the Markov chain Monte Carlo sampling algorithms are also given. The proposed method is illustrated by three simulation examples and a real dataset.

PMID:37163496 | DOI:10.1371/journal.pone.0285277

JMIR Med Educ. 2023 May 10;9:e38079. doi: 10.2196/38079.

ABSTRACT

BACKGROUND: Electronic health records (EHRs) play a substantial role in modern health care, especially during prerounding, when residents gather patient information to inform daily care decisions of the care team. The effective use of the EHR system is crucial for efficient and frustration-free prerounding. Ideally, the system should be designed to support efficient user interactions by presenting data effectively and providing easy navigation between different pages. Additionally, training on the system should aim to make user interactions more efficient by familiarizing the users with best practices that minimize interaction time while using the full potential of the system’s capabilities. However, formal training on EHR systems often falls short of providing residents with all the necessary EHR-related skills, leading to the adoption of inefficient practices and the underuse of the system’s full range of capabilities.

OBJECTIVE: This study aims to examine the efficiency of EHR use during prerounding among pediatric residents, assess the effect of experience level on EHR use, and identify areas for improvement in EHR design and training.

METHODS: A mixed methods approach was used, involving a self-reported survey and video analysis of prerounding practices of the entire population of pediatric residents from a large teaching hospital in the South Atlantic Region. The residents were stratified by experience level by postgraduate year. Data were collected on the number of pages accessed, duration of prerounding, task completion rates, and effective use of data sources. Observational and qualitative data complemented the quantitative analysis. Our study followed the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) reporting guidelines, ensuring completeness and transparency of reporting.

RESULTS: Of the 30 pediatric residents, 20 were included in the analyses; of these, 16 (80%) missed at least 1 step during prerounding. Although more experienced residents on average omitted fewer steps, 4 (57%) of the 7 most experienced residents still omitted at least 1 step. On average, residents took 6.5 minutes to round each patient and accessed 21 pages within the EHR during prerounding; no statistically significant differences were observed between experience levels for prerounding times (P=.48) or number of pages accessed (P=.92). The use of aggregated data pages within the EHR system neither seem to improve prerounding times nor decrease the number of pages accessed.

CONCLUSIONS: The findings suggest that EHR design should be improved to better support user needs, and hospitals should adopt more effective training programs to familiarize residents with the system’s capabilities. We recommend implementing prerounding checklists and providing ongoing EHR training programs for health care practitioners. Despite the generalizability of limitations of our study in terms of sample size and specialization, it offers valuable insights for future research to investigate the impact of EHR use on patient outcomes and satisfaction, as well as identify factors that contribute to efficient and effective EHR usage.

PMID:37163346 | DOI:10.2196/38079

Environ Toxicol. 2023 May 10. doi: 10.1002/tox.23812. Online ahead of print.

ABSTRACT

BACKGROUND: Papillary thyroid carcinoma (PTC) is one of the most widespread malignant tumors of the endocrine system, with a high incidence. Budding uninhibited by benzimidazoles 1 (BUB1), one of the spindle assembly checkpoint (SAC) genes, is a multitask protein kinase required for eukaryotic chromosome segregation. Although BUB1 has been explored in several types of cancer, its biological role and molecular mechanisms in PTC remain unclear.

METHODS: In this study, we performed an examination of four public datasets along with local PTC cohorts and discovered that BUB1 was elevated in PTC compared to non-cancer tissues. High BUB1 expression was linked with the status of BRAF^V600E , RAS, and TERT after statistical analysis.

RESULTS: Clinically, BUB1 is associated with a variety of clinicopathological features in PTC patients. Interestingly, analysis of the TCGA database showed that BUB1 was closely associated with poor prognosis of PTC and significantly correlated with PFS. As determined by regression analysis, BUB1, and T stage were independent predictors of PTC and were related to BRAF^V600E and lymph node metastatic status. By RT-qPCR, BUB1 was considerably overexpressed in PTC cell lines in comparison with normal thyroid epithelial cells.

CONCLUSION: We confirmed that the knockdown of BUB1 in BCPAP and TPC1 cell lines significantly inhibited cell proliferation, cloning, and migration in vitro experiments. These results imply that BUB1 may be a significant oncogenic gene that is directly associated with the prognosis of PTC and may represent a future target for therapeutic intervention.

PMID:37163344 | DOI:10.1002/tox.23812

JMIR Res Protoc. 2023 May 10;12:e42417. doi: 10.2196/42417.

ABSTRACT

BACKGROUND: Opioid use disorder (OUD) during pregnancy presents a significant risk to maternal, fetal, and neonatal health, increasing the likelihood of adverse events, such as maternal overdose, pregnancy loss, stillbirth, preterm birth, low birth weight, and neonatal abstinence syndrome. In order to reduce the risk of these outcomes, the standard of care for OUD during pregnancy in many jurisdictions within the United States and Canada is opioid agonist therapy (OAT). OAT refers to prescription medications that alleviate or eliminate opioid withdrawal symptoms, so that opioid use can be managed more safely. Although OAT has been recognized as a safe option for pregnant people with OUD, many jurisdictions do not have treatment guidelines regarding pharmacological options, dosing recommendations, side effect management, and individual preferences. There is currently a lack of systematic evidence on the impacts of different OAT regimens on pregnancy outcomes.

OBJECTIVE: We aim to evaluate the impacts of specific OAT agents on pregnancy outcomes and inform recommendations for practitioners treating pregnant people with OUD.

METHODS: The MEDLINE, Embase, CINAHL, and PsycINFO databases will be searched for published quantitative studies assessing pregnancy outcomes for individuals on OAT. Given the substantially increased risk of preterm birth, low birth weight, small for gestational age, and stillbirth among pregnant people with OUD, these four end points will comprise our primary outcomes. Database searches will not be restricted by date, and conference abstracts will be restricted to the past 2 years. Titles, abstracts, and full-text articles will be independently screened by 2 reviewers. Data will be extracted independently and in duplicate, using a data extraction form to reduce the risk of reviewer bias. The risk of bias within individual studies will be assessed by using the appropriate CASP (Critical Appraisal Skills Programme) checklists. For studies that consider the same research questions, interventions, or outcomes, meta-analyses will be conducted to synthesize the pooled effect size. In the event that studies cannot be compared directly, results will be synthesized in a narrative account. Between-study heterogeneity will be measured by using the τ² statistic. If more than 10 studies are available for pooling, publication bias will be evaluated by using the Egger regression test.

RESULTS: As of January 2023, a total of 3266 abstracts have been identified for screening. Data extraction is expected to commence in February 2023.

CONCLUSIONS: The topic of OAT and its effect on pregnancy is an understudied area that has the potential to improve health outcomes, clinical practice, education, and community advocacy. The results of our review will be used to inform clinical practice guidelines and improve health outcomes for pregnant people. Findings will be disseminated to diverse groups of stakeholders, including policy makers, clinicians, community partners, and individuals with lived experience of drug use.

TRIAL REGISTRATION: PROSPERO CRD42022332082; https://tinyurl.com/2p94pkx5.

INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): DERR1-10.2196/42417.

PMID:37163329 | DOI:10.2196/42417

Mol Biol Cell. 2023 May 10:mbcE23030077. doi: 10.1091/mbc.E23-03-0077. Online ahead of print.

ABSTRACT

During embryonic development dramatic cell shape changes and movements re-shape the embryonic body plan. These require robust but dynamic linkage between the cell-cell adherens junctions and the force-generating actomyosin cytoskeleton. Our view of this linkage has evolved, and we now realize linkage is mediated by mechanosensitive multiprotein complexes assembled via multivalent connections. Here we combine genetic, cell biological and modeling approaches to define the mechanism of action and functions of an important player, Drosophila Polychaetoid, homolog of mammalian ZO-1. Our data reveal that Pyd reinforces cell junctions under elevated tension, and facilitates cell rearrangements. Pyd is important to maintain junctional contractility and in its absence cell rearrangements stall. We next use structured illumination microscopy to define the molecular architecture of cell-cell junctions during these events. The cadherin-catenin complex and Cno both localize to puncta along the junctional membrane, but are differentially enriched in different puncta. Pyd, in contrast, exhibits a distinct localization to strands that extend out from the region occupied by core junction proteins. We then discuss the implications for the protein network at the junction-cytoskeletal interface, suggesting different proteins localize and function in distinct ways, perhaps in distinct subcomplexes, but combine to produce robust connections. [Media: see text] [Media: see text].

PMID:37163320 | DOI:10.1091/mbc.E23-03-0077

JAMA Psychiatry. 2023 May 10. doi: 10.1001/jamapsychiatry.2023.1162. Online ahead of print.

ABSTRACT

IMPORTANCE: General and specific factors of psychopathology are associated with future adverse outcomes, indicating that they might be useful for identifying individuals at greatest risk. However, it remains unknown if these associations are attributable to confounders that may influence both the psychopathology factors and later outcomes.

OBJECTIVE: To analyze associations between psychopathology factors and clinically relevant outcomes within family pairs, adjusting for unmeasured confounds by applying co-twin control and sibling comparison designs.

DESIGN, SETTING, AND PARTICIPANTS: This longitudinal cohort study with a follow-up range of 9 to 13 years included all Swedish twins born from 1959 to 1985 who participated in the Study of Twin Adults: Genes and Environment (60% response rate) and the oldest pair of all Swedish siblings born from 1959 to 1985 per the Multi-Generation Register. Twins were evaluated based on responses to a hierarchical factor model derived using multivariate statistics. Sibling pairs were evaluated based on psychiatric diagnoses per the Swedish National Patient Register. Information on outcome events and prescriptions were derived from the National Patient Register, Prescribed Drug Register, and Crime Register. Baseline assessment was in August 2005, and data were analyzed from January 2022 to February 2023.

EXPOSURES: Hierarchical factor model consisting of 1 general and 4 specific factors fit to 48 psychiatric symptoms on which twin participants self-reported in 2005 and 1 general and 3 specific factors fit to 9 register-based psychiatric diagnoses assigned to sibling participants prior to 2005.

MAIN OUTCOMES AND MEASURES: Outcomes consisted of 7 register-based events occurring after 2005, including suicidal behavior, substance overdoses, and criminal suspicion or convictions (data available until the end of 2013), and prescription of antidepressants, antialcohol or antiopioid medication, antipsychotics, and stimulants (data available until the end of 2017).

RESULTS: The study included 32 328 twins (mean [SD] age, 34 [8] years; 16 076 [49.73%] male) and 1 942 106 siblings (mean [SD] age, 34 [7] years; 991 500 [51.05%] male). General psychopathology was significantly associated with all 7 outcomes within sibling pairs (mean within-pair odds ratio [OR], 2.28; 95% CI, 2.19-2.37) and dizygotic twin pairs (within-pairs OR, 1.65; 95% CI, 1.38-1.98) and with 3 outcomes within monozygotic twin pairs (mean within-pairs OR, 1.77; 95% CI, 1.35-2.36). Within sibling pairs, the specific internalizing factor was associated with antidepressant prescriptions (within-pairs OR, 1.65; 95% CI, 1.59-1.71), the specific substance misuse factor was associated prescription of antialcohol and antiopioid medication (within-pairs OR, 2.36; 95% CI, 2.20-2.54), and the specific psychotic factor was associated with antipsychotic medications (within-pairs OR, 1.61; 95% CI, 1.51-1.72). Similar results emerged within twin pairs.

CONCLUSION AND RELEVANCE: In this cohort study, general psychopathology was significantly associated with all 7 outcomes within sibling and dizygotic twin pairs and 3 outcomes within monozygotic twin pairs at 10 years. Within twin and sibling pairs, the specific factors were primarily associated with related outcomes. Several of the associations in this cohort study could not be attributed to unmeasured confounds shared by family members, suggesting that interventions toward broad psychopathology dimensions might help reduce the risk of future clinically relevant events.

PMID:37163290 | DOI:10.1001/jamapsychiatry.2023.1162