Category: Nevin Manimala

Environ Res. 2023 May 1:115983. doi: 10.1016/j.envres.2023.115983. Online ahead of print.

ABSTRACT

The bio-based nanoparticles synthesis and assessment of their potential biomedical applications related research is rapidly emerging. The ability of an aqueous ethanolic bark extract of Mangifera indica to synthesize silver nanoparticles (AgNPs) as well as its antibacterial, anti-inflammatory, and anticancer activities were investigated in this study. Interestingly, the bark extract effectively synthesized the AgNPs, including an absorbance peak at 412 nm and sizes ranging from 56 to 89 nm. The Fourier Transform Infrared spectroscopy (FTIR) analysis confirmed that the presence of most essential functional groups belongs to the most bioactive compounds. Synthesized AgNPs showed fine antibacterial activity against the Urinary Tract Infection (UTI) causing bacterial pathogens such as Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus saprophyticus at 50 μg mL^-1 concentrations. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against these pathogens were found as 12.5 ± 0.8 & 13 ± 0.6, 13.6 ± 0.5 & 14 ± 0.7, 11.5 ± 0.3 & 11.5 ± 0.4, 13 ± 0.8 & 13 ± 0.7, and 11.8 ± 0.4 & 12 ± 0.8 μg mL^-1 respectively. Interestingly, this AgNPs also possesses outstanding anti-inflammatory and anticancer activities as studied against the egg albumin denaturation (85%) inhibition and MCF 7 (Michigan Cancer Foundation-7: breast cancer cells) cell line (cytotoxicity: 80.1%) at 50 μg mL^-1 concentration. Similarly at 50 μg mL^-1 concentration showed 75% of DPPH radical scavenging potential. These activities were dose dependent, and the findings suggest that the M. indica bark aqueous ethanolic extract synthesized AgNPs can be used as antibacterial, anti-inflammatory, and anticancer agents after in-vivo testing.

PMID:37137456 | DOI:10.1016/j.envres.2023.115983

Int J Radiat Oncol Biol Phys. 2023 May 1:S0360-3016(23)00371-1. doi: 10.1016/j.ijrobp.2023.04.010. Online ahead of print.

ABSTRACT

BACKGROUND/PURPOSE: Intermediate-risk prostate cancer is a heterogeneous disease state with diverse treatment options. The 22-gene Decipher genomic classifier (GC) retrospectively has shown to improve risk stratification in these patients. Herein, we assess the performance of the GC in men with intermediate-risk disease enrolled on xxxx with updated follow-up.

METHODS: After National Cancer Institute approval, biopsy slides were collected from xxxx, a randomized phase III trial of men with intermediate-risk prostate cancer randomized to 70.2 Gy vs 79.2 Gy of radiotherapy without androgen deprivation therapy. RNA was extracted from the highest-grade tumor foci to generate the locked 22-gene GC model. The primary endpoint for this ancillary project was disease progression (composite of biochemical-, local-failure, distant metastasis, prostate cancer-specific mortality, and use of salvage therapy). Individual endpoints were also assessed. Fine-Gray or cause-specific Cox multivariable models were constructed adjusting for randomization arm and trial stratification factors.

RESULTS: Two-hundred and fifteen patient samples passed quality control for analysis. The median follow-up was 12.8 years (range 2.4-17.7). On multivariable analysis, the 22-gene GC (per 0.1 unit) was independently prognostic for disease progression (subdistribution hazard ratio [sHR] 1.12, 95%CI 1.00-1.26, p=0.04), biochemical failure (sHR 1.22, 95%CI 1.10-1.37, p<0.001), distant metastasis (sHR 1.28, 95%CI 1.06-1.55, p=0.01), and PCSM (sHR 1.45, 95%CI 1.20-1.76, p<0.001). 10-year distant metastasis in GC low patients was 4% compared to 16% for GC high risk patients. In patients with lower GC scores, the 10-year difference in metastasis-free survival rate between arms was -7%, compared to 21% for higher GC patients (p-interaction 0.04).

CONCLUSIONS: This study represents the first validation of a biopsy-based gene expression classifier, assessing both its prognostic and predictive value, using data from a randomized phase III trial of intermediate-risk prostate cancer. Decipher improves risk stratification and can aid in treatment decision-making in men with intermediate-risk disease.

PMID:37137444 | DOI:10.1016/j.ijrobp.2023.04.010

Rev Esp Cardiol (Engl Ed). 2023 May 1:S1885-5857(23)00112-3. doi: 10.1016/j.rec.2023.04.003. Online ahead of print.

ABSTRACT

INTRODUCTION AND OBJECTIVES: Despite medical advances, mortality after ST-elevation myocardial infarction (STEMI) remains high. Women are often underrepresented in trials and registries, limiting knowledge of their management and prognosis. It is unknown whether life expectancy in women of all ages treated with primary percutaneous coronary intervention (PPCI) is similar to that in a reference population free of the disease. The main objective of this study was to determine whether life expectancy in women undergoing PPCI and surviving the main event returns to a similar level to that in the general population of the same age and region.

METHODS: We included all patients diagnosed with STEMI from January 2014 to October 2021. We matched women to a reference population of the same age and region from the National Institute of Statistics to determine observed survival, expected survival, and excess mortality (EM) using the Ederer II method. We repeated the analysis in women aged ≤ 65 and > 65 years.

RESULTS: A total of 2194 patients were recruited, of whom 528 were women (23.9%). In women surviving the first 30 days, EM at 1, 5 and 7 years was 1.6% (95%CI, 0.3-4), 4.7% (95%CI, 0.3-10.1), and 7.2% (95%CI, 0.5-15.1), respectively.

CONCLUSIONS: EM was reduced in women with STEMI who were treated with PPCI and who survived the main event. However, life expectancy remained lower than that in a reference population of the same age and region.

PMID:37137427 | DOI:10.1016/j.rec.2023.04.003

Int J Cardiol. 2023 May 1:S0167-5273(23)00592-2. doi: 10.1016/j.ijcard.2023.04.038. Online ahead of print.

ABSTRACT

Guidelines recommend managing patients aged ≥75 with non-ST-segment elevation myocardial infarction (NSTEMI) similar to younger patients. We analyze disparities in NSTEMI management and compare those ≥80 years to those <80 years. This is a matched case-control study using the 2016 National Inpatient Sample data of adults with NSTEMI receiving percutaneous coronary intervention with drug-eluting stent (PCI-DES) – one artery or no intervention. We included the statistically significant variables in univariate analysis in exploratory multivariate logistic regression models. Total sample included 156,328 patients, out of which 43,265 were ≥ 80 years, and 113,048 were < 80 years. Patients ≥80 years were more likely to not have an intervention (73.3%) when compared to those <80 (44.1%), P < 0.0005. Regardless of age, PCI-DES-one artery improved survival compared to no intervention (Age < 80: OR 0.230, 95% CI 0.189-0.279, and ≥ 80: OR 0.265, 95% CI 0.195-0.361, P < 0.0005). Women (OR 0.785, 95% CI 0.766-0.804, P < 0.0005) and non-white race (OR 0.832, 95% CI 0.809-0.855, P < 0.0005) were less likely to receive an intervention. Non-Medicare/Medicaid insurance was associated with 40% lower likelihood of dying in <80 age group (OR 0.596, 95% CI 0.491-0.724, P < 0.0005), and 16% higher chance of intervention overall (OR 1.160, 95% CI 1.125-1.197, P < 0.0005). Patients aged ≥80 with NSTEMI were 29% less likely to receive an intervention compared to patients aged <80, even though patients >80 derived similar mortality benefits from the intervention. There were gender, payor, and race-based disparities in NSTEMI management in 2016.

PMID:37137356 | DOI:10.1016/j.ijcard.2023.04.038

Int J Biol Macromol. 2023 May 1:124673. doi: 10.1016/j.ijbiomac.2023.124673. Online ahead of print.

ABSTRACT

Magnetic drug targeting can be a strategy for effectively delivering phytochemicals in cancer treatment. Here, we demonstrate the benefit of magnetic targeting with superparamagnetic iron oxide nanoparticles for cytotoxicity enhancement of lutein (LUT) against breast cancer cells. Fabrication of LUT-loaded chitosan/alginate iron oxide nanoparticles (LUT-CS/Alg-Fe₃O₄-NPs) was optimized by a statistical approach using response surface methodology based on the Box-Behnken design. The optimized LUT-CS/Alg-Fe₃O₄-NPs with a balance among LUT concentration, copolymer coating, and iron ion concentration exhibited controlled size, narrow size distribution, better crystallinity, excellent saturation magnetization, and sustained-release profile. The negligible magnetic coercivity and remanent magnetization confirmed the superparamagnetism of the prepared NPs. The optimized LUT-CS/Alg-Fe₃O₄-NPs were biocompatible while exhibiting a significantly enhanced cytotoxicity towards breast cancer MCF-7 cells upon exposure to a permanent magnet compared to free LUT with a 4-fold increase, suggesting the potential of LUT-CS/Alg-Fe₃O₄-NPs as magnetically targeted delivery for breast cancer.

PMID:37137353 | DOI:10.1016/j.ijbiomac.2023.124673

Parasitol Int. 2023 May 1:102755. doi: 10.1016/j.parint.2023.102755. Online ahead of print.

ABSTRACT

Temporal variation of the helminth infracommunity structure in the Gafftopsail pompano Trachinotus rhodopus was studied during bi-monthly revisions of samples collected offshore from Puerto Ángel, Oaxaca (Mexican Pacific) in 2018. In total, 110 specimens of T. rhodopus were subjected to a parasitic review. Helminths found were identified to the lowest possible taxonomic level (six species and three genera) by means of morphological and molecular data. Attributes of the helminth infracommunities are described through statistical analyses, showing stability in terms of their richness throughout the year. However, variations were found in helminth abundance related to the seasonality of samplings, which may be associated with the life cycles of the parasites, the host species’ gregarious behavior, the availability of intermediate hosts, and/or the diet of T. rhodopus.

PMID:37137347 | DOI:10.1016/j.parint.2023.102755

Addict Behav. 2023 Apr 26;144:107743. doi: 10.1016/j.addbeh.2023.107743. Online ahead of print.

ABSTRACT

BACKGROUND: For Black men of middle-age, the overdose mortality statistics are increasingly dire. To better understand the severity of the crisis, we estimated the cumulative risk of drug overdose deaths among non-Hispanic Black men in mid-life using a period life table approach. We report the chances of Black men aged 45 years dying of a drug overdose before age 60.

METHODS: A period life table reflects what would happen to a hypothetical cohort if it experienced the prevailing age-specific probabilities of death. In our hypothetical cohort, we followed 100,000 non-Hispanic Black men aged 45 years for 15 years. All-cause death probabilities were obtained from the National Center for Health Statistics (NCHS) 2021 life table series. Overdose mortality rates were obtained from the National Vital Statistics System through the Wide-Ranging Online Data for Epidemiologic Research of the Centers for Disease Control and Prevention (CDC WONDER) database. We also constructed a period life table for a comparison group of White men.

RESULTS: The life table shows that, for Black men who are 45 years of age in the United States, 1 in 52 (nearly 2%) is expected to die of drug overdose before reaching age 60, if current mortality rates persist. For White men, the estimate is 1 in 91 men (about 1%). The life table also shows that from age 45 to 59 years, the number of overdose deaths increased in the cohort of Black men but decreased in White men.

CONCLUSIONS: This study extends our understanding of the immense loss to Black communities from the preventable drug deaths of Black men in middle-age.

PMID:37137170 | DOI:10.1016/j.addbeh.2023.107743

Homoeopath Physician. 1887 Feb;7(2):66.

NO ABSTRACT

PMID:37135858 | PMC:PMC9717041

ACS Appl Mater Interfaces. 2023 May 3. doi: 10.1021/acsami.3c02247. Online ahead of print.

ABSTRACT

Top and bottom interfaces of high-χ cylinder-forming polystyrene-block-maltoheptaose (PS-b-MH) diblock copolymer (BCP) thin films are manipulated using cross-linked copolymer underlayers and a fluorinated phase-preferential surface-active polymer (SAP) additive to direct the self-assembly (both morphology and orientation) of BCP microdomains into sub-10 nm patterns. A series of four photo-cross-linkable statistical copolymers with various contents of styrene, a 4-vinylbenzyl azide cross-linker, and a carbohydrate-based acrylamide are processed into 15 nm-thick cross-linked passivation layers on silicon substrates. A partially fluorinated analogue of the PS-b-MH phase-preferential SAP additive is designed to tune the surface energy of the top interface. The self-assembly of PS-b-MH thin films on top of different cross-linked underlayers and including 0-20 wt % of SAP additive is investigated by atomic force microscopy and synchrotron grazing incidence small-angle X-ray scattering analysis. The precise manipulation of the interfaces of ca. 30 nm thick PS-b-MH films not only allows the control of the in-plane/out-of-plane orientation of hexagonally packed (HEX) cylinders but also promotes epitaxial order-order transitions from HEX cylinders to either face-centered orthorhombic or body-centered cubic spheres without modifying the volume fraction of both blocks. This general approach paves the way for the controlled self-assembly of other high-χ BCP systems.

PMID:37134266 | DOI:10.1021/acsami.3c02247

Arch Pathol Lab Med. 2023 May 4. doi: 10.5858/arpa.2022-0268-OA. Online ahead of print.

ABSTRACT

CONTEXT.—: Noninvasive papillary urothelial carcinomas (PUCs) comprise most urinary bladder tumors. Distinction between low-grade (LG-PUC) and high-grade (HG-PUC) PUCs is pivotal for determining prognosis and subsequent treatment.

OBJECTIVE.—: To investigate the histologic characteristics of tumors with borderline features between LG-PUC and HG-PUC, focusing on the risk of recurrence and progression.

DESIGN.—: We reviewed the clinicopathologic parameters of noninvasive PUC. Tumors with borderline features were subcategorized as follows: tumors that look like LG-PUC but have occasional pleomorphic nuclei (1-BORD-NUP) or elevated mitotic count (2-BORD-MIT), and tumors with side-by-side distinct LG-PUC and less than 50% HG-PUC (3-BORD-MIXED). Recurrence-free, total progression-free, and specific invasion-free survival curves were derived from the Kaplan-Meier method, and Cox regression analysis was performed.

RESULTS.—: A total of 138 patients with noninvasive PUC were included, with the following distribution: LG-PUC (n = 52; 38%), HG-PUC (n = 34; 25%), BORD-NUP (n = 21; 15%), BORD-MIT (n = 14; 10%) and BORD-MIXED (n = 17; 12%). Median (interquartile range) follow-up was 44.2 months (29.9-73.1 months). Invasion-free survival was different between the 5 groups (P = .004), and pairwise comparison showed that HG-PUC had a worse prognosis compared with LG-PUC (P ≤ .001). On univariate Cox analysis, HG-PUC and BORD-NUP were 10.5 times (95% CI, 2.3-48.3; P = .003) and 5.9 times (95% CI, 1.1-31.9; P = .04) more likely to invade, respectively, when compared to LG-PUC.

CONCLUSIONS.—: Our findings confirm a continuous spectrum of histologic changes in PUC. Approximately a third of noninvasive PUCs show borderline features between LG-PUC and HG-PUC. Compared with LG-PUC, BORD-NUP and HG-PUC were more likely to invade on follow-up. BORD-MIXED tumors did not statistically behave differently from LG-PUC.

PMID:37134243 | DOI:10.5858/arpa.2022-0268-OA