Category: Nevin Manimala

Vet World. 2022 Jun;15(6):1550-1556. doi: 10.14202/vetworld.2022.1550-1556. Epub 2022 Jun 27.

ABSTRACT

BACKGROUND AND AIM: Infectious bovine rhinotracheitis (IBR) is an infectious disease widely distributed globally and is considered the main cause of various reproductive and respiratory tract diseases in cattle and buffaloes. This study aimed to estimate seroprevalence and determine risk factors associated with the presentation of IBR in the municipality of Sotaquirá, Boyacá (Colombia).

MATERIALS AND METHODS: A descriptive cross-sectional study with simple random sampling was performed, and the sample size was 1,000 cattle. Blood samples were obtained by coccygeal venipuncture and processed through indirect enzyme-linked immunosorbent assay using the Synbiotics^® kit (Zoetis, New Jersey, USA) with a sensitivity and specificity of 96% and 98%, respectively. Data were processed using the statistical program EpiInfo^® (Centers for Disease Control and Prevention; Atlanta, Georgia).

RESULTS: A high seroprevalence of 57.5% was established. Seroprevalence was the highest in cattle >4 years of age (65.0% apparent seroprevalence [AS]; 67% true seroprevalence [TS]) and in the Holstein breed (65.5% AS; 67.8% TS). The breed and age of the animals were significantly associated with each other. The Holstein breed, age group >4 years, uncertified semen, and fetal death were established as risk factors for IBR. In comparison, the age groups of <1 and 1-2 years and the Normande breed were established as protective factors against the bovine herpesvirus-1 virus.

CONCLUSION: Management factors, such as livestock from other owners and animal purchases, which affect disease presentation, are evident. The implementation and development of novel prevention and control measures for IBR at the national level are necessary.

PMID:35993084 | PMC:PMC9375214 | DOI:10.14202/vetworld.2022.1550-1556

Vet World. 2022 Jun;15(6):1595-1600. doi: 10.14202/vetworld.2022.1595-1600. Epub 2022 Jun 30.

ABSTRACT

BACKGROUND AND AIM: Acute kidney injury (AKI) is associated with a grave prognosis. A clinical assessment of kidney function can be performed based on the glomerular filtration rate (GFR). Cystatin C (CysC) can indicate the GFR or kidney function and its measurement is currently performed using immunological methods such as nephelometry, immunoturbidimetry, and enzyme-linked immunosorbent assays in human medicine. However, these techniques are not specific for use in veterinary medicine. This study aimed to validate an immunoturbidimetric assay for serum CysC (sCy) in dogs, determine the sCy reference intervals for healthy dogs, evaluate sCy stability in serum samples, and compare sCy with serum creatinine (sCr) in healthy dogs and dogs with AKI.

MATERIALS AND METHODS: Forty-three dogs were divided into a control group (n = 19) and an AKI group (n = 24). An immunoturbidimetric method including commercially available human CysC calibrated with canine CysC was used to evaluate canine serum samples.

RESULTS: An average recovery of 97% was observed for canine serum samples. The reference interval for CysC in healthy dogs was 0.57-1.29 mg/L. The sCy concentration in dogs with AKI was significantly higher (2.82 ± 1.46 mg/L) than in healthy dogs (0.93 ± 0.18 mg/L). Statistical analysis confirmed a strong correlation between sCy and sCr (r = 0.94; p < 0.05) in dogs with AKI.

CONCLUSION: The immunoturbidimetric method of evaluating sCy yielded satisfactory results and can be used for canine samples when a species-specific calibrator is used. Furthermore, sCy is a reliable marker of renal dysfunction in dogs. It is best to store samples for sCy evaluation at temperatures between 4°C and 8°C.

PMID:35993081 | PMC:PMC9375203 | DOI:10.14202/vetworld.2022.1595-1600

Vet World. 2022 Jun;15(6):1408-1412. doi: 10.14202/vetworld.2022.1408-1412. Epub 2022 Jun 8.

ABSTRACT

BACKGROUND AND AIM: Diabetes mellitus is a carbohydrate metabolism disorder produced mainly by a deficit in insulin production or insulin resistance. The homeostatic model assessment (HOMA) is a broad method for estimating insulin resistance and β-cell function. This study aimed to evaluate the stages of insulin resistance using non-linear HOMA index analysis in normoglycemic normal weight and obese canines.

MATERIALS AND METHODS: Insulin resistance was evaluated using the mathematical HOMA non-linear model in canines with different body and glycemic conditions. Forty canines were studied, including 20 normoglycemic normal weight canines and 20 normoglycemic obese canines. Chi-square statistical test was applied, in which the body condition and HOMA non-linear index were evaluated. The Spearman correlation test was conducted to evaluate the glycemic and insulin variables in both types of canines.

RESULTS: The Spearman correlation presented a correlation between increased blood glucose levels and insulin in obese canines, with a correlation of 0.79, while no significant changes in insulin were found in normal weight canines with different blood glucose levels, with a correlation of -0.11. The analysis of the non-linear HOMA index showed significant differences between non-linear HOMA insulin resistance in normal weight and obese canines, with a Chi-square statistic of 16.9424 and p = 0.000039. Canine with increased HOMA 2 showed higher levels of insulin with increasing blood glucose compared to those with normal HOMA 2.

CONCLUSION: The HOMA 2 is a marker for evaluating increased insulin resistance in obese dogs and can be used to determine patients at risk for glycemic alterations.

PMID:35993079 | PMC:PMC9375204 | DOI:10.14202/vetworld.2022.1408-1412

Vet World. 2022 Jun;15(6):1423-1429. doi: 10.14202/vetworld.2022.1423-1429. Epub 2022 Jun 9.

ABSTRACT

BACKGROUND AND AIM: Maedi-visna is a chronic viral disease of sheep with worldwide distribution causing substantial economic losses to the small ruminant industry. Pneumonia and mastitis are the main manifestations of the disease. This study aimed to investigate the occurrence of maedi-visna virus (MVV) in sheep using histopathology and nested polymerase chain reaction (PCR) techniques and also to estimate the seroprevalence of small ruminant lentiviruses (SRLVs) in sheep and goats using commercially available enzyme-linked immunosorbent assay (ELISA).

MATERIALS AND METHODS: Lung tissue samples from 380 sheep were collected and fixed in 10% formalin for histopathology and molecular diagnosis of MVV. Separately, 806 serum samples were randomly collected from 633 sheep and 173 goats to detect the seroprevalence of SRLVs using ELISA.

RESULTS: The results showed that 4.7% of lung samples (n=190) were positive by both histopathology and nested PCR, 5.8% (n = 380) were positive by histopathology only (have lymphoid follicular hyperplasia), and 7.4% (n = 190) were positive by nested PCR only. Statistical analysis revealed a moderate agreement between the two tests (Kappa=0.451, n = 190). Serology results revealed that sheep and/or goats herd prevalence was 59.8% (n = 87), while individual seroprevalence in sheep (40.1%, n = 633) was significantly higher than that in the other six countries and also significantly higher than that in goats (18.5%, n = 173) (at p < 0.05).

CONCLUSION: The moderate statistical agreement between nested PCR and histopathological diagnosis of MVV in formalin-fixed paraffin-embedded sheep lung tissue samples (Kappa=0.451, n = 190) suggests combining both tests for more sensitive MVV detection in sheep lung samples. SRLVs seropositivity in sheep was significantly higher than in goats, thus, it is of high concern and urges the inquiry into the economic impact of the disease and the financial benefit of adopting eradication measures.

PMID:35993078 | PMC:PMC9375225 | DOI:10.14202/vetworld.2022.1423-1429

Vet World. 2022 Jun;15(6):1506-1514. doi: 10.14202/vetworld.2022.1506-1514. Epub 2022 Jun 18.

ABSTRACT

BACKGROUND AND AIM: Salmonellosis is an infectious disease that often occurs in chickens and is caused by Salmonella enteritidis. The use of antibiotics to prevent this disease can result in the development of resistance in pathogenic bacteria, in addition to the presence of antibiotic residues in consumed carcasses. Red ginger (Zingiber officinale var. rubrum) has active compounds that potentially act as immunomodulators which increase specific and non-specific immune responses through the induction of cytokine production. This study was conducted to determine the effects of red ginger powder mixed in feed for starter and finisher broiler chickens, based on the evaluation of the expression of immunoglobulin A (IgA), histopathologic description of the ileum and cecum, IgA, and immunoglobulin Y (IgY) expression in the spleen, and the isolation count of S. enteritidis in fresh fecal samples.

MATERIALS AND METHODS: A total of 100 starter and 100 finisher Cobb broiler chickens were divided into four groups, designated as T0, T1, T2, and T3, respectively: Group T0 was fed commercial feed with no added 2% red ginger powder or S. enteritidis induction, and served as a negative control; Group T1 was inoculated with a 0.25 mL S. enteritidis oral induction (1 × 10⁷ colony-forming unit [CFU] [0.5 McFarland standard]), and served as a positive control; Group T2 was fed with feed containing 2% red ginger powder; while Group T3 was fed with feed containing 2% red ginger powder and was orally inoculated with S. enteritidis with a dose similar to T1. The normal feed was given on the 1^st-7^th days. The mixture of 2% red ginger powder was given on the 7^th-15^th days. The S. enteritidis was induced on the 15^th day (1 × 10⁷ CFU). Necropsy was performed on the 16^th day and tissues were fixed in 10% formalin and routinely processed for histopathologic and immunohistochemical analyses. The data were analyzed using a one-way analysis of variance test, Tukey’s analysis, and the Mann-Whitney U non-parametric statistical analysis test.

RESULTS: The 2% red ginger powder was found to significantly (p < 0.05) increase IgA expression and additionally decrease tissue damage in the cecum and ileum. It also increased IgA and IgY expression in the spleen. In addition, a decrease was observed in the S. enteritidis number isolated from finisher fresh feces, but none was found in the isolated starter fresh feces.

CONCLUSION: These findings indicate that the addition of red ginger powder to chicken feed is a potential natural immunomodulator against S. enteritidis infection.

PMID:35993070 | PMC:PMC9375218 | DOI:10.14202/vetworld.2022.1506-1514

Vet World. 2022 Jun;15(6):1438-1448. doi: 10.14202/vetworld.2022.1438-1448. Epub 2022 Jun 12.

ABSTRACT

BACKGROUND AND AIM: Fasciolosis is a significant problem in veterinary and public health, causing huge economic losses. Epidemiological studies of fasciolosis in dairy cattle in Indonesia are few and existing reports primarily focus on prevalence. This study aimed to determine the prevalence, risk factors, and infection intensity of fasciolosis in dairy cattle in Boyolali, Indonesia.

MATERIALS AND METHODS: This cross-sectional study included 400 dairy cattle from 72 household farms in eight subdistricts. Fecal samples (n=400) were examined using the Flukefinder^® kit and the simple sedimentation technique was the gold standard for fasciolosis. In-person interviews using questionnaires collected data on farmers, farms, and animal characteristics. Chi-square and logistic regression analyses were performed to evaluate the associated risk factors for fasciolosis, and p < 0.05 was considered statistically significant.

RESULTS: The overall prevalence of fasciolosis in dairy cattle in Boyolali, Indonesia, was 16.50% (95% confidence interval [CI] 12.85-20.15) at the animal level (n = 400), whereas 40.28% at household farms (n = 72) level (95% CI 18.67-51.88). The relative sensitivity and specificity of the Flukefinder^® kit compared with those of the gold standard were 79.49% and 92.52%, respectively, with a moderate agreement (kappa=0.59; p < 0.001). Fasciolosis was more likely in cattle originating from the Mojosongo subdistrict than from other subdistricts (odds ratio (OR)=5.28, 95% CI 1.22-22.94); from farms that did not process manure versus from those that did (OR = 3.03, 95% CI 1.43-4.71); and with farmers that had never attended extension programs compared with those who had (OR = 4.72, 95% CI 1.99-11.19). Studied cattle were mostly affected by light Fasciola spp. infections (92.4%, 95% CI 77.8-100%) followed by moderate (6.1%, 95% CI 0-22.2%) and heavy (1.5%, 95% CI 0-5.6%) infections.

CONCLUSION: Fasciolosis is prevalent in dairy cattle in Boyolali, Indonesia. Control efforts should target the high-risk Mojosongo subdistrict, emphasize the importance of processing manure, and encourage farmers to attend extension programs. Flukefinder^® is a practical on-site diagnostic kit for fasciolosis in Indonesian dairy farms. Parasite species identification and a malacological survey of intermediate hosts of Fasciola spp. in the farming environment are required for further research.

PMID:35993064 | PMC:PMC9375217 | DOI:10.14202/vetworld.2022.1438-1448

Biomed Res Int. 2022 Aug 12;2022:2719016. doi: 10.1155/2022/2719016. eCollection 2022.

ABSTRACT

OBJECTIVE: To explore the application of pulmonary ultrasound in visual nursing of artificial airway in patients with acute respiratory distress syndrome (ARDS).

METHODS: Seventy-eight ARDS patients with mechanical ventilation admitted from February 2021 to January 2022 were included and divided into the intervention group and the control group. The control group was given routine airway nursing, and the intervention group was given visual airway nursing management through lung ultrasound. The arterial blood gas analysis indexes, mechanical ventilation time, ICU treatment time, total hospitalization time, aspiration, and the incidence of ventilator-associated pneumonia (VAP) were compared between the two groups.

RESULTS: After treatment, PaO₂, PaCO₂, SPO₂, and oxygenation indexes were significantly improved compared with those before treatment, and the indexes in the intervention group were better than those in the control group after treatment, and the differences were statistically significant (P < 0.05). The mechanical ventilation time (5.39 ± 0.68 vs. 7.92 ± 0.59 days), ICU treatment time (8.05 ± 1.14 vs. 10.71 ± 1.16 days), and total hospitalization time (12.05 ± 2.20 vs. 15.68 ± 2.18 days) in the intervention group were significantly shorter than those in the control group (P < 0.05). The incidences of aspiration (2.56% vs. 15.38%) and VAP (5.13% vs. 20.51%) in the intervention group was significantly lower than that in the control group (P < 0.05).

CONCLUSION: The application of visual artificial airway management assisted by lung ultrasound in ARDS patients can shorten the treatment time and hospitalization time of mechanical ventilation, reduce the incidence of aspiration and VAP, and improve the prognosis of patients.

PMID:35993058 | PMC:PMC9391190 | DOI:10.1155/2022/2719016

Biomed Res Int. 2022 Aug 12;2022:6161410. doi: 10.1155/2022/6161410. eCollection 2022.

ABSTRACT

BACKGROUND: Malaria is endemic in tropical and subtropical regions and causes up to one million deaths each year. It mostly affects sub-Saharan African countries including Ethiopia. In Ethiopia, it was estimated that about 75% of the land and 68% of the population are exposed to malaria. The aim of the current study was to determine malaria cases, its impact on the level of hemoglobin, and parasitemia and predisposing factors among febrile patients who visited Sibu Sire Health Care centers.

METHODS: Institution-based cross-sectional study was undertaken from May to October, 2020. Febrile patients who visited Sibu Sire Health Care centers were purposefully selected as the target population for the present study. Blood samples were collected and thick and thin smears stained with Giemsa. Hemoglobin level was determined using HemoCue Hb 301. Structured questionnaire and SPSS statistical software were used to collect and analyze data. P value < 0.05 was stated as statistically significant.

RESULTS: The overall prevalence of malaria was 168/979 (17.2%) out of which Plasmodium falciparum was 132/168 (78.5%), Plasmodium vivax was 27/168 (16.1%), and mixed infection was 9/168 (5.4%). To assess factors associated with dependent variable and determine the strength of association, binary logistic regression was used at adjusted odds ratios with 95% confidence intervals. The associations between malaria cases, hemoglobin levels, and parasitemia were estimated to evaluate the impacts of malaria on hemoglobin levels and parasitemia level. Out of 979 febrile patients (male 453 and female 526), 168 (male 99 and female 69) individuals were infected with Plasmodium species and Plasmodium falciparum were the predominant parasites. The majority of the study participants 144/168 (85.7%) were from rural residences. Highest malaria-infected patients 74/168 (44%) were aged between 15 and 30 years old. The level of hemoglobin and parasitemia was highly associated with malaria cases; hence, in this study area, intensities of hemoglobin and parasitemia are significantly associated with Plasmodium species.

CONCLUSION: There was a moderate prevalence of malaria parasitemia and hemoglobin level among patients visiting Sibu Sire Health Care center; however, it needs further intervention to prevent and control malaria transmission in this malaria hotspot area.

PMID:35993053 | PMC:PMC9391188 | DOI:10.1155/2022/6161410

Biomed Res Int. 2022 Aug 8;2022:7948898. doi: 10.1155/2022/7948898. eCollection 2022.

ABSTRACT

As a family of transcription factors, the correlations between expression pattern of nine interferon regulatory factor (IRF) family members, the immune invasion pattern, and the associated patient survival rate in endometrial carcinoma (EC) remain to be elucidated. Based on The Cancer Genome Atlas (TCGA), the expression profiles of the high and low IRF mRNA expression groups were analyzed using R (3.6.3) statistical software. Gene annotation and pathway analyses were performed using Metascape. GSEA was performed using the R package clusterProfiler (3.6.3). The single-sample gene set enrichment analysis (ssGSEA) was used to quantify the relative tumor infiltration levels of immune cell types. Immunohistochemistry data provided by HPA database was used to study the expression of the IRF proteins. Using the GEPIA dataset, the correlation between the expression of IRFs and the tumor stage of EC was analyzed. The correlations between the different IRFs were analyzed using cBioPortal. The expression of IRF2, IRF3, IRF5, IRF6, IRF7, IRF8, and IRF9 was different when comparing EC and normal endometrial samples. IRF2, IRF6, IRF7, and IRF8 were indicated to be potential diagnostic markers for EC. In combination with receiver operating characteristic analysis results, IRF2, IRF6, IRF7, and IRF8 were indicated to be potential diagnostic markers for EC. Levels of individual IRFs were associated with alternate outcomes, with the expression of IRF3 being correlated with the stage of EC and high expression of IRF4 being positively correlated with overall survival (OS); conversely, high expression of IRF5 was negatively correlated with OS. Additionally, high expression levels of both IRF2 and IRF4 were positively correlated with the disease-specific survival rate, and high expression of IRF4 was positively correlated with the progression-free interval. These data suggest a role for IRF2, IRF4, and IRF5 in the prognosis of EC. The expression of IRFs is associated with immune infiltration.

PMID:35993041 | PMC:PMC9381850 | DOI:10.1155/2022/7948898

Oxid Med Cell Longev. 2022 Aug 10;2022:8691830. doi: 10.1155/2022/8691830. eCollection 2022.

ABSTRACT

Osteoporotic fracture, a major complication which is known as the outcome postmenopausal osteoporosis, seriously threatens the health of postmenopausal women. At present, the traditional osteoporotic fracture prediction methods are characterized by inconvenient application and time-consuming statistical results, while predictive serum biomarkers can make up for this shortcoming. Accurate and advanced risk prediction of osteoporotic fracture is meaningful to early prevention and intervention, effectively avoiding the risk of this disease and the secondary fracture in the surgical treatment. In this study, based on the BEYOND cohort, a 2-year follow-up study was conducted after subjects participated to survey if OF occurred. Independent sample t-test and Mann-Whitney U-test were used to analyze the differences of bone metabolism biomarkers between the OF and non-OF group. Cox proportional hazard model was used to screen the potential biomarkers might be used to predict OF risk. ROC curves and AUCs were used to analyze the predictive accuracy, and the Delong’s test was used to compare the differences between the AUCs. 15 postmenopausal women with low bone mass and OF were found, and other 60 subjects without OF were matched with 1 : 4, age, and BMI classification as control group. The serum IL-6 (OR = 1.139, 95%CI = 1.058 – 1.226) and leptin (OR = 0.921, 95%CI = 0.848 – 1.000) were found as OF risk predictive biomarkers for postmenopausal women with low bone mass with high accuracy (IL – 6 = 0.871) (leptin = 0.813) and accuracy enhanced when they were combined (AUC = 0.898). The results of Delong’s test showed that the difference of AUC between leptin and IL-6&Leptin was meaningful (P = 0.024) but meaningless between IL-6 and leptin (P = 0.436), IL-6 and IL-6&Leptin (P = 0.606). To sum up, IL-6 and leptin are the predictive biomarkers of OF for postmenopausal women with low bone mass. The IL-6 can improve the prediction accuracy of leptin (P = 0.024), but not vice versa (P = 0.606). Trial Information. Registered on the Chinese Clinical Trial Registry already. (Registration Number: ChiCTR-SOC-17013090).

PMID:35993023 | PMC:PMC9385352 | DOI:10.1155/2022/8691830