Category: Nevin Manimala

Am J Dermatopathol. 2023 May 2. doi: 10.1097/DAD.0000000000002446. Online ahead of print.

ABSTRACT

Granulomas are composed of a heterogeneous population of resident and recruited macrophages according to the type of lesion, extent of injury, and local tissue environment (eg, involved site and interaction with infiltrating lymphocytes). Although macrophage phenotypes in various types of granulomas have been previously described, the experimental conditions varied across studies, precluding a comparative and comprehensive understanding of granulomas. This study was conducted to comparatively analyze the expression of markers of the M1 and M2 phenotypes in macrophages that compose various types of granulomas, including epithelioid lesions, under strict conditions. Surgical specimens of cutaneous sarcoidosis (11 lesions), suture granuloma (10 lesions), and subcutaneous lipogranuloma (12 lesions) were immunohistochemically stained for CD11c, CD206, CD163, and CD10. The expression of these markers in macrophages composing each type of granuloma was scored and statistically analyzed. Granuloma macrophages were mostly immunoreactive for CD11c and CD206 in all the examined cases, although many intermingling CD206-negative cells were observed in 5 cases of lipogranuloma. CD163 and CD10 were diffusely expressed in macrophages composing suture granuloma and lipogranuloma, whereas they were not expressed in epithelioid cells in cutaneous sarcoidosis. Meanwhile, “interstitial” macrophages around epithelioid granulomas revealed moderate to marked CD163 expression in 7 lesions of cutaneous sarcoidosis. These results indicate significant differences of expression of CD163 and CD10 between cutaneous sarcoidosis and suture granuloma/lipogranuloma; CD163 and CD10 are downregulated after the epithelioid transformation of macrophages in cutaneous sarcoidosis.

PMID:37130221 | DOI:10.1097/DAD.0000000000002446

Cell Mol Biol (Noisy-le-grand). 2022 Dec 31;68(12):12-16. doi: 10.14715/cmb/2022.68.12.3.

ABSTRACT

Group A rotavirus (RVA) is the most important foodborne pathogen and is transmitted by oysters. The purpose of this study was to monitor and evaluate the prevalence of RVA in oysters collected in Oualidia Lagoon, Morocco from March 2018 to March 2019 and its relationship to watershed contribution. To achieve this, 312 oysters from 26 lots were collected and tested for viral contamination using real-time polymerase chain reaction. The results showed that 46.15% of the tested samples were positive for RVA. The seasonal distribution of RVA was statistically significant. This is a warning signal against the potential health risks in this oyster growing area, following the consumption of oysters contaminated with RVA, which presents a huge threat to human health resulting in serious illnesses of gastroenteritis.

PMID:37130188 | DOI:10.14715/cmb/2022.68.12.3

Cell Mol Biol (Noisy-le-grand). 2022 Dec 31;68(12):21-25. doi: 10.14715/cmb/2022.68.12.5.

ABSTRACT

Vitamin D deficiency is a hot topic as it affects about 50% of the world’s population. Beyond its extra-osseous effects, notably on the cardiovascular system. In recent years many epidemiological studies have looked at the role of vitamin D status in modulating blood pressure. It appears that there is an inverse association between serum vitamin D levels and blood pressure. It is in this context that our prospective study on the Moroccan population allows having global knowledge of the association between vitamin D and arterial hypertension. This is a descriptive study of vitamin D status in Moroccan hypertensive patients in whom blood samples were taken for serum 25(OH)D determination. The data collected were anonymised and entered into SPSS software. X2 and ANOVA tests were used to investigate the existence of correlations between vitamin D status and age, age at menopause, body mass index, sex and hypertension. 1015 hypertensive patients were included in this study with a female predominance of 84.2%, mainly over 50 years old in 80.8%, of whom 31.5% were overweight or obese, 13.2% had a hypertensive parent and 70.8% had vitamin D deficiency. Vitamin D regulates at least 3% of the human genome with receptors throughout the body, including vascular smooth muscle cells, vascular endothelium and cardiomyocytes, where it acts by vasodilation and by blocking the renin-angiotensin-aldosterone system (RAAS) to lower blood pressure. There is a relationship between low serum vitamin D levels and high blood pressure. Our study concluded that there is an association between hypovitaminosis D and the pathology of hypertension. However, further randomised studies are needed and in the meantime, clinicians could propose it in the therapeutic arsenal of Moroccan hypertensive patients.

PMID:37130186 | DOI:10.14715/cmb/2022.68.12.5

Cell Mol Biol (Noisy-le-grand). 2022 Dec 31;68(12):32-35. doi: 10.14715/cmb/2022.68.12.7.

ABSTRACT

The objective of this study was to analyze the detection value of D-dimer, C-reactive protein, ESR and blood routine in the perioperative treatment of orthopaedic trauma patients. For this aim, 86 orthopedic trauma patients who were treated at our hospital from December 2020 to December 2021 were selected as the study group, including 40 patients in the infected group and 46 patients in the uninfected group. 86 healthy people who were examined in our hospital were selected as the control group. D-dimer, C-reactive protein, ESR and blood routine test were given to the patients in the study group to evaluate the detection value of D-dimer, C-reactive protein, ESR and blood routine test in the perioperative treatment of orthopaedic trauma patients. Results indicated that compared with the control group, the levels of D-dimer, C-reactive protein, erythrocyte sedimentation rate, white blood cell and neutrophil in the study group were higher than those in the control group, With statistical difference (P < 0.05); Compared with the uninfected group, the levels of D-dimer, C-reactive protein, erythrocyte sedimentation rate, white blood cell and neutrophil in the infected group were higher than those in the uninfected group, With statistical difference (P < 0.05). Conclusion: Using D-dimer, C-reactive protein, ESR and blood routine to treat orthopaedic trauma patients in the perioperative period has good clinical detection value.

PMID:37130183 | DOI:10.14715/cmb/2022.68.12.7

PLoS One. 2023 May 2;18(5):e0283547. doi: 10.1371/journal.pone.0283547. eCollection 2023.

ABSTRACT

BACKGROUND: In developing countries, home delivery increases the risk of maternal and perinatal mortality. Despite this, home deliveries account for a considerable share of deliveries in developing nations such as Ethiopia. Evidence on factors that affect homebirth is required for the measures needed to overcome these conditions.

OBJECTIVE: To identify predictors of homebirth among women attending health facilities in Wondo Genet, Sidama Region.

METHODS: Unmatched case-control study was conducted from May to June 2021 among 308 mothers (102 cases and 206 controls) who recently delivered and visited either postnatal care or sought immunization service at public health facilities of Wondo Genet. A structured interviewer-administered questionnaire was used to collect data. Epi-Data version 3.1 was used for data entry, and the Statistical Package for the Social Sciences (SPSS) version 20 was used for data analysis. Bivariate and multivariate logistic regression analyses were used to identify the determinants of homebirth. The association between the outcome variable and independent variables was declared statistically significant at a P-value < 0.05 with a 95% Confidence Interval (CI) in a multivariable model.

RESULTS: Rural residence [AOR: 3.41; 95%CI: 1.58-7.39], lifetime physical IPV [AOR: 2.35; 95%CI: 1.06-5.17], grand-multiparity [AOR: 5.36; 95%CI: 1.68-17.08], non-use of contraception before recent pregnancy [AOR: 5.82; 95%CI: 2.49-13.60], >30 min to reach health facility [AOR: 2.14; 95%CI: 1.02-4.51], and lack of facemask [AOR: 2.69; 95%CI: 1.25-5.77] were statistically significant predictors of homebirth.

CONCLUSION AND RECOMMENDATION: The access gap to maternity services should be narrowed between rural and urban women. Healthcare programs concerning women’s empowerment could help reduce persistent intimate partner violence. Family planning needs to be promoted, and multiparous women should be counseled on the adverse obstetric consequences of homebirth. The devastating effect of the coronavirus disease 2019 pandemic on maternity services should be prevented.

PMID:37130142 | DOI:10.1371/journal.pone.0283547

PLoS One. 2023 May 2;18(5):e0284117. doi: 10.1371/journal.pone.0284117. eCollection 2023.

ABSTRACT

BACKGROUND: Chronic diseases are considered one of the major causes of illness, disability, and death worldwide. Chronic illness leads to a huge health and economic burden, especially in low- and middle-income countries. This study examined disease-stratified healthcare utilisation (HCU) among Bangladesh patients with chronic diseases from a gender perspective.

METHODS: Data from the nationally representative Household Income and Expenditure Survey 2016-2017 consisting of 12,005 patients with diagnosed chronic diseases was used. Gender differentiated chronic disease stratified-analytical exploration was performed to identify the potential factors to higher or lower utilisation of healthcare services. Logistic regression with step-by-step adjustment for independent confounding factors was the method used.

RESULTS: The five most prevalent chronic diseases among patients were gastric/ulcer (Male/Female, M/F: 16.77%/16.40%), arthritis/rheumatism (M/F: 13.70%/ 13.86%), respiratory diseases/asthma/bronchitis (M/F: 12.09% / 12.55%), chronic heart disease (M/F: 8.30% / 7.41%), and blood pressure (M/F: 8.20% / 8.87%). Eighty-six percent of patients with chronic diseases utilised health care services during the previous 30 days. Although most patients received outpatient healthcare services, a substantial difference in HCU among employed male (53%) and female (8%) patients were observed. Chronic heart disease patients were more likely to utilise health care than other disease types, which held true for both genders while the magnitude of HCU was significantly higher in males (OR = 2.22; 95% CI:1.51-3.26) than their female counterparts (OR = 1.44; 1.02-2.04). A similar association was observed among patients with diabetes and respiratory diseases.

CONCLUSION: A burden of chronic diseases was observed in Bangladesh. Patients with chronic heart disease utilised more healthcare services than patients experiencing other chronic diseases. The distribution of HCU varied by patient’s gender as well as their employment status. Risk-pooling mechanisms and access to free or low-cost healthcare services among the most disadvantaged people in society might enhance reaching universal health coverage.

PMID:37130132 | DOI:10.1371/journal.pone.0284117

PLoS Comput Biol. 2023 May 2;19(5):e1010989. doi: 10.1371/journal.pcbi.1010989. eCollection 2023 May.

ABSTRACT

Animals rely on different decision strategies when faced with ambiguous or uncertain cues. Depending on the context, decisions may be biased towards events that were most frequently experienced in the past, or be more explorative. A particular type of decision making central to cognition is sequential memory recall in response to ambiguous cues. A previously developed spiking neuronal network implementation of sequence prediction and recall learns complex, high-order sequences in an unsupervised manner by local, biologically inspired plasticity rules. In response to an ambiguous cue, the model deterministically recalls the sequence shown most frequently during training. Here, we present an extension of the model enabling a range of different decision strategies. In this model, explorative behavior is generated by supplying neurons with noise. As the model relies on population encoding, uncorrelated noise averages out, and the recall dynamics remain effectively deterministic. In the presence of locally correlated noise, the averaging effect is avoided without impairing the model performance, and without the need for large noise amplitudes. We investigate two forms of correlated noise occurring in nature: shared synaptic background inputs, and random locking of the stimulus to spatiotemporal oscillations in the network activity. Depending on the noise characteristics, the network adopts various recall strategies. This study thereby provides potential mechanisms explaining how the statistics of learned sequences affect decision making, and how decision strategies can be adjusted after learning.

PMID:37130121 | DOI:10.1371/journal.pcbi.1010989

Int J Soc Determinants Health Health Serv. 2023 May 2:27551938231170834. doi: 10.1177/27551938231170834. Online ahead of print.

ABSTRACT

This study aimed to assess the coverage of essential postnatal maternal care services among women residing in the slums of Islamabad. A community-based, cross-sectional study was conducted to assess the coverage of essential postnatal care (PNC) services. Using random sampling, 416 women living in the squatter settlements of Islamabad Capital Territory were selected as study participants. Data was analyzed by using SPSS version 22. Descriptive statistics were employed to display frequencies for categorical variables, whereas mean, median, and standard deviation were calculated for continuous variables. The analysis of data showed that 93.5 percent of the women utilized postnatal services at least once after delivery. Approximately 9 percent and 4 percent of women received all eight recommended services within 24 h of birth and beyond 24 h of birth, respectively. Effective PNC services were received by only 1 percent of the women. The study revealed that the utilization of effective PNC was very low. The majority of the women delivered in health institutions and received their first PNC checkups, but follow-up for the recommended checkups was very low. These results can help health professionals and policymakers in designing programs and developing efficient strategies that would improve PNC service utilization in Pakistan.

PMID:37130119 | DOI:10.1177/27551938231170834

PLoS One. 2023 May 2;18(5):e0285168. doi: 10.1371/journal.pone.0285168. eCollection 2023.

ABSTRACT

Prediction of virus-host protein-protein interactions (PPI) is a broad research area where various machine-learning-based classifiers are developed. Transforming biological data into machine-usable features is a preliminary step in constructing these virus-host PPI prediction tools. In this study, we have adopted a virus-host PPI dataset and a reduced amino acids alphabet to create tripeptide features and introduced a correlation coefficient-based feature selection. We applied feature selection across several correlation coefficient metrics and statistically tested their relevance in a structural context. We compared the performance of feature-selection models against that of the baseline virus-host PPI prediction models created using different classification algorithms without the feature selection. We also tested the performance of these baseline models against the previously available tools to ensure their predictive power is acceptable. Here, the Pearson coefficient provides the best performance with respect to the baseline model as measured by AUPR; a drop of 0.003 in AUPR while achieving a 73.3% (from 686 to 183) reduction in the number of tripeptides features for random forest. The results suggest our correlation coefficient-based feature selection approach, while decreasing the computation time and space complexity, has a limited impact on the prediction performance of virus-host PPI prediction tools.

PMID:37130110 | DOI:10.1371/journal.pone.0285168

PLoS One. 2023 May 2;18(5):e0285087. doi: 10.1371/journal.pone.0285087. eCollection 2023.

ABSTRACT

OBJECTIVES: Plasma leakage, a hallmark of disease in Dengue virus (DENV) infection, is an important clinical manifestation and is often associated with numerous factors such as viral factors. The aim of this study is to investigate the association of virus serotype, viral load kinetics, history of infection, and NS1 protein with plasma leakage.

METHODS: Subjects with fever ≤ 48 hours and positive DENV infection were included. Serial laboratory tests, viral load measurements, and ultrasonography examination to assess plasma leakage were performed.

RESULTS: DENV-3 was the most common serotype found in the plasma leakage group (35%). Patients with plasma leakage demonstrated a trend of higher viral load and a longer duration of viremia compared to those without. This was significantly observed on the fourth day of fever (p = 0.037). We found higher viral loads on specific days in patients with plasma leakage in both primary and secondary infections compared to those without. In addition, we also observed more rapid viral clearance in patients with secondary infection. NS1 protein, especially after 4 days of fever, was associated with higher peak viral load level, even though it was not statistically significant (p = 0.470). However, pairwise comparison demonstrated that peak viral load level in the group of patients with circulating NS1 detected for 7 days was significantly higher than the 5-day group (p = 0.037).

CONCLUSION: DENV-3 was the most common serotype to cause plasma leakage. Patients with plasma leakage showed a trend of higher viral load and a longer duration of viremia. Higher level of viral load was observed significantly on day 5 in patients with primary infection and more rapid viral clearance was observed in patients with secondary infection. Longer duration of circulating NS1 protein was also seen to be positively correlated with higher peak viral load level although not statistically significant.

PMID:37130105 | DOI:10.1371/journal.pone.0285087