Category: Nevin Manimala

Sci Rep. 2021 May 4;11(1):9502. doi: 10.1038/s41598-021-88924-y.

ABSTRACT

The E2F family of transcription factors is important for many cellular processes, from their canonical role in cell cycle regulation to other roles in angiogenesis and metastasis. Alteration of the Rb/E2F pathway occurs in various forms of cancer, including breast cancer. E2F1 ablation has been shown to decrease metastasis in MMTV-Neu and MMTV-PyMT transgenic mouse models of breast cancer. Here we take a bioinformatic approach to determine the E2F1 regulated genomic alterations involved in the metastatic cascade, in both Neu and PyMT models. Through gene expression analysis, we reveal few transcriptome changes in non-metastatic E2F1^-/- tumors relative to transgenic tumor controls. However investigation of these models through whole genome sequencing found numerous differences between the models, including differences in the proposed tumor etiology between E2F1^-/- and E2F1^+/+ tumors induced by Neu or PyMT. For example, loss of E2F1 within the Neu model led to an increased contribution of the inefficient double stranded break repair signature to the proposed etiology of the tumors. While the SNV mutation burden was higher in PyMT mouse tumors than Neu mouse tumors, there was no statistically significant differences between E2F WT and E2F1 KO mice. Investigating mutated genes through gene set analysis also found a significant number of genes mutated in the cell adhesion pathway in E2F1^-/- tumors, indicating this may be a route for disruption of metastasis in E2F1^-/- tumors. Overall, these findings illustrate the complicated nature of uncovering drivers of the metastatic process.

PMID:33947907 | DOI:10.1038/s41598-021-88924-y

Sci Rep. 2021 May 4;11(1):9525. doi: 10.1038/s41598-021-89046-1.

ABSTRACT

Currently, there is no clear consensus in cataract surgery if low compared to high energy femto-lasers may enable better capsulotomy quality and induce lower inflammatory response. Therefore, the aim of this study was to compare the intra-operative outcomes achieved with high and low pulse energy femtosecond laser systems for cataract surgery. The charts of 200 eyes of 200 patients aged 68.3 ± 10.3 years who had undergone femtosecond laser-assisted cataract surgery using either group 1 high pulse energy: LenSx (Alcon Laboratories) (N = 100) or group 2 low pulse energy: FEMTO LDV Z8 (Ziemer) (N = 100) laser were reviewed retrospectively. Integrity of anterior capsulotomy, classified as (1) complete (free-floating or with minor microadhesions), (2) incomplete and (3) with capsular tears, intraoperative completeness of the clear corneal incisions (CCI, main incision and side port), incidences of intraoperative miosis and incidence of subconjunctival hemorrhage were evaluated and compared between the two groups. The proportion of complete capsulotomies was significantly higher in the group 2 than the group 1 (100% vs 94%; p = 0.03). The incidences of intraoperative miosis (0% vs 19%) and subconjunctival hemorrhage (1% vs 63%) were significantly lower in the group 2 than the group 1 (p < 0.001). Completeness of the main incision was comparable (97% vs 95%; p = 0.721) between the two groups. Although not statistically significant, the completeness of side-port incision was slightly better in the group 2 than the group 1 (91% vs 86%). Low energy laser system performed significantly better in terms of completeness of capsulotomy, intraoperative miosis and sub-conjunctival hemorrhage, compared with high energy laser; the CCI outcomes were comparable.

PMID:33947910 | DOI:10.1038/s41598-021-89046-1

Sci Rep. 2021 May 4;11(1):9868. doi: 10.1038/s41598-021-89721-3.

NO ABSTRACT

PMID:33947947 | DOI:10.1038/s41598-021-89721-3

Sci Rep. 2021 May 4;11(1):9491. doi: 10.1038/s41598-021-88927-9.

ABSTRACT

Obesity increases the risk of developing cardiovascular disease and other metabolic diseases. We intended to compare three different anthropometric indicators of obesity, in predicting the incidence of cardiovascular events in Chinese type 2 diabetes. Beijing Community Diabetes Study was a prospective multi-center study conducted in Beijing community health centers. Type 2 diabetes patients from fourteen community health centers were enrolled at baseline. The primary endpoint was cardiovascular events. The upper quartile of neck circumference (NC) was set as greater NC. A total of 3299 diabetes patients were enrolled. In which, 941 (28.52%) had cardiovascular disease at baseline. Logistic analysis showed that central obesity (waist circumference (WC) above 90 cm in men and 85 cm in women) and greater NC were all related to baseline cardiovascular disease (adjusted OR = 1.49, and 1.55). After 10-year follow-up, 340 (10.31%) had cardiovascular events. Compared with patients without cardiovascular events, those having cardiovascular events had higher BMI, larger WC and NC. Cox regression analysis showed that greater WC and NC were all associated with the occurrence of cardiovascular events (adjusted HR = 1.41, and 1.38). A higher NC and WC might increase the risk of cardiovascular events by about 40% in type 2 diabetes patients in Beijing communities.

PMID:33947884 | DOI:10.1038/s41598-021-88927-9

Sci Rep. 2021 May 4;11(1):9455. doi: 10.1038/s41598-021-88931-z.

ABSTRACT

Of all the characteristics of a violin, those that concern its shape are probably the most important ones, as the violin maker has complete control over them. Contemporary violin making, however, is still based more on tradition than understanding, and a definitive scientific study of the specific relations that exist between shape and vibrational properties is yet to come and sorely missed. In this article, using standard statistical learning tools, we show that the modal frequencies of violin tops can, in fact, be predicted from geometric parameters, and that artificial intelligence can be successfully applied to traditional violin making. We also study how modal frequencies vary with the thicknesses of the plate (a process often referred to as plate tuning) and discuss the complexity of this dependency. Finally, we propose a predictive tool for plate tuning, which takes into account material and geometric parameters.

PMID:33947897 | DOI:10.1038/s41598-021-88931-z

Sci Rep. 2021 May 4;11(1):9501. doi: 10.1038/s41598-021-88581-1.

ABSTRACT

In this study, we aimed to develop and validate a machine learning-based mortality prediction model for hospitalized heat-related illness patients. After 2393 hospitalized patients were extracted from a multicentered heat-related illness registry in Japan, subjects were divided into the training set for development (n = 1516, data from 2014, 2017-2019) and the test set (n = 877, data from 2020) for validation. Twenty-four variables including characteristics of patients, vital signs, and laboratory test data at hospital arrival were trained as predictor features for machine learning. The outcome was death during hospital stay. In validation, the developed machine learning models (logistic regression, support vector machine, random forest, XGBoost) demonstrated favorable performance for outcome prediction with significantly increased values of the area under the precision-recall curve (AUPR) of 0.415 [95% confidence interval (CI) 0.336-0.494], 0.395 [CI 0.318-0.472], 0.426 [CI 0.346-0.506], and 0.528 [CI 0.442-0.614], respectively, compared to that of the conventional acute physiology and chronic health evaluation (APACHE)-II score of 0.287 [CI 0.222-0.351] as a reference standard. The area under the receiver operating characteristic curve (AUROC) values were also high over 0.92 in all models, although there were no statistical differences compared to APACHE-II. This is the first demonstration of the potential of machine learning-based mortality prediction models for heat-related illnesses.

PMID:33947902 | DOI:10.1038/s41598-021-88581-1

NPJ Sci Food. 2021 May 4;5(1):9. doi: 10.1038/s41538-021-00091-6.

ABSTRACT

Achieving food security is a critical challenge of the Anthropocene that may conflict with environmental and societal goals such as increased energy access. The “fuel versus food” debate coupled with climate mitigation efforts has given rise to next-generation biofuels. Findings of this systematic review indicate just over half of the studies (56% of 224 publications) reported a negative impact of bioenergy production on food security. However, no relationship was found between bioenergy feedstocks that are edible versus inedible and food security (P value = 0.15). A strong relationship was found between bioenergy and type of food security parameter (P value < 0.001), sociodemographic index of study location (P value = 0.001), spatial scale (P value < 0.001), and temporal scale (P value = 0.017). Programs and policies focused on bioenergy and climate mitigation should monitor multiple food security parameters at various scales over the long term toward achieving diverse sustainability goals.

PMID:33947871 | DOI:10.1038/s41538-021-00091-6

Sci Data. 2021 May 4;8(1):125. doi: 10.1038/s41597-021-00906-x.

ABSTRACT

Storm surges are among the deadliest coastal hazards and understanding how they have been affected by climate change and variability in the past is crucial to prepare for the future. However, tide gauge records are often too short to assess trends and perform robust statistical analyses. Here we use a data-driven modeling framework to simulate daily maximum surge values at 882 tide gauge locations across the globe. We use five different atmospheric reanalysis products for the storm surge reconstruction, the longest one going as far back as 1836. The data that we generate can be used, for example, for long-term trend analyses of the storm surge climate and identification of regions where changes in the intensity and/or frequency of storms surges have occurred in the past. It also provides a better basis for robust extreme value analysis, especially for tide gauges where observational records are short. The data are made available for public use through an interactive web-map as well as a public data repository.

PMID:33947872 | DOI:10.1038/s41597-021-00906-x

Eur J Phys Rehabil Med. 2021 May 5. doi: 10.23736/S1973-9087.21.06595-3. Online ahead of print.

ABSTRACT

BACKGROUND: Physical Therapists may use the Graston technique to relieve soft-tissue restrictions and pain in the upper cervical region. However, no studies have tested the efficacy of the Graston technique in patients with cervicogenic headache.

AIM: To investigate the effect of adding the Graston technique to an exercise program on pain intensity, neck disability, cervical range of motion, headache frequency and duration, and medication intake in subjects with cervicogenic headache.

DESIGN: A single-blinded(assessor), randomized controlled trial.

SETTING: Outpatient rehabilitation clinic.

POPULATION: Sixty patients,35 to 50 years old, with cervicogenic headache have participated in the study. Patients were recruited from the outpatient clinics of Tala general hospital in Menoufia governorate.

METHODS: Patients were assigned randomly into two groups. Patients in the study group received the Graston technique in addition to an exercise program, while patients in the control group received the exercise program only. All patients received 3 sessions a week for 4 weeks. The primary outcome measure was the visual analogue scale(VAS), while the secondary outcome measures were neck disability index (NDI), cervical range of motion(CROM), headache frequency and duration, and medication intake. All outcomes were measured at baseline, after 2 weeks as well as after 4 weeks of intervention.

RESULTS: After 2 weeks of the treatment, statistically significant differences were found in all the measured outcomes (p< 0.05) in favor of the Graston group except for neck extension(p=0.08). After 4 weeks of the intervention, statistically significant differences were found in all the measured outcomes (p< 0.05) in favor of the Graston group.

CONCLUSIONS: Short-term effect of using the Graston technique in combination with an exercise program can reduce pain, decrease headache frequency and duration, and medication intake more than the exercise program alone in the medium-term. However, although the study results for NDI and neck ROM were statistically significant, it is neither clinically relevant change nor clearly outside the range of measurement error.

PMID:33947825 | DOI:10.23736/S1973-9087.21.06595-3

Eur J Phys Rehabil Med. 2021 May 5. doi: 10.23736/S1973-9087.21.06621-1. Online ahead of print.

ABSTRACT

BACKGROUND: Fatigue in Parkinson’s Disease (PD) compromises patients’ physical activity and poses questions on how to plan correct rehabilitation training. In addition, the relationship between subjective perceived fatigue and fatigue in motor performance is not yet entirely understood. As a consequence, a conclusive interpretation of muscular mechanisms of fatigue in PD has not yet been achieved. Among the various instrumental evaluations for fatigue, multichannel surface electromyography (sEMG) is a recognized tool that permits the study of myoelectric manifestations of fatigue.

AIM: To assess if muscles in PD show a different myoelectric fatigue pattern compared to the muscles of healthy age-matched subjects.

DESIGN: Observational controlled study.

POPULATION: Idiopathic Parkinson’s Disease, Hohen &Yahr II and III stage, Parkinsonian Fatigue Scale average score ≥ 2.95, no therapy modification in the 4 weeks preceding the study; exclusion criteria: Mini Mental State Examination ≤ 24, upper limb disease/symptoms that might interfere with sEMG analysis, presence of other fatiguerelated conditions. Twenty patients were selected according to these criteria. Twenty untrained healthy subjects were matched.

METHODS: All subjects underwent a muscle fatigue protocol and a sEMG analysis of the right biceps brachii muscle during electrically stimulated and voluntary contractions in order to obtain a myoelectric fatigue muscular pattern. The myoelectric pattern is characterized by the modifications of the following sEMG variables: reduction of mean frequency and muscle fibre conduction velocity, increase of average rectified value.

RESULTS: No statistical differences where observed between groups for sEMG variables and muscular electric behaviour in all contraction conditions (all p> .05).

CONCLUSIONS: Our results show that parkinsonian muscles does not differ from the muscles of healthy age-matched subjects in developing peripheral myoelectric fatigue. Nevertheless, the role of fatigue perception at rest and particularly during physical activity must be clearly understood in order to further target the rehabilitative approach for fatigued parkinsonian patients and to reduce hypomobility.

CLINICAL REHABILITATION IMPACT: In rehabilitative terms, these findings allow us to highlight the possibility of performing sustained training with isometric contractions in PD subjects; therefore, fatigue “per se” does not constitute a barrier for the execution of muscular exercises, likewise intensive.

PMID:33947826 | DOI:10.23736/S1973-9087.21.06621-1