Category: Nevin Manimala

Mol Neurobiol. 2025 Dec 10;63(1):280. doi: 10.1007/s12035-025-05561-6.

ABSTRACT

ASD etiology may be influenced by non-coding single-nucleotide polymorphisms (SNPs) in 3′ untranslated regions (3’UTRs). These variations can affect post-transcriptional regulation by altering RNA structure and miRNA binding patterns. PTEN and TSC1, two key regulators of the PI3K/AKT/mTOR signaling pathway, are promising candidate genes for ASD. We performed a case-control study involving 108 individuals diagnosed with ASD and 184 healthy matched controls from the Tunisian population. Four specific 3’UTR SNPs (PTEN: rs701848, rs34140758; TSC1: rs739442, rs2809244) were analyzed through genotyping. Statistical associations were assessed using various genetic inheritance models, with multivariate logistic regression adjusting for gender, family psychiatric history, and parental age. A thorough bioinformatics approach was applied, which included miRNASNP-v3 for predicting altered miRNA binding sites, RNAhybrid for calculating the minimum free energy (MFE) of miRNA-mRNA duplexes, PhyloP and phastCons for evolutionary conservation analysis, and mfold for modeling RNA secondary structures. PTEN rs701848 showed a significant statistical association with ASD risk that remained significant after correction for multiple testing (corrected p < .0004), while TSC1 rs739442 showed a suggestive association (p = .009, corrected p = .036). Systematic functional assessment using multi-criteria evaluation identified rs701848 as having high predicted functional impact (extensive miRNA binding changes, including loss of neurodevelopmental miR-129 sites, high conservation (PhyloP = 2.24), and altered RNA stability), while rs739442 showed moderate impact, and rs2809244 minimal predicted functional significance. For mutant alleles, RNA structure modeling showed slight decreases in mRNA stability, which might affect transcript accessibility to regulatory factors. Evolutionary conservation analysis demonstrated that rs701848 and rs34140758 reside in highly conserved areas, whereas rs739442 and rs2809244 are in less evolutionarily conserved regions. By integrating population-genetics data with bioinformatics predictions, this study supports the relevance of non-coding SNPs as biological risk factors for ASD. It emphasizes their functional significance in modulating post-transcriptional gene regulation mechanisms.

PMID:41369821 | DOI:10.1007/s12035-025-05561-6

Ann Surg Oncol. 2025 Dec 10. doi: 10.1245/s10434-025-18862-w. Online ahead of print.

ABSTRACT

BACKGROUND: Lung cancer, especially non-small cell lung cancer (NSCLC), is a leading cause of cancer death. Surgical resection is the primary treatment for early-stage NSCLC. Video-assisted thoracoscopic surgery (VATS) and robot-assisted thoracic surgery (RATS) are minimally invasive options, with RATS offering better precision at higher costs. This meta-analysis compared the perioperative outcomes of both techniques in NSCLC.

METHODS: A systematic search in PubMed, Web of Science, NLM, CNKI, and Wanfang was conducted for RCTs published between 31 July 2021 and 31 December 2024. Primary outcomes included operation time, blood loss, lymph node dissection, conversion to thoracotomy, chest drainage time, and hospital stay. Statistical analyses were performed using RevMan 5.3, with heterogeneity assessed using the I² statistic. A p value lower than 0.05 was considered significant.

RESULTS: Three RCTs were analyzed. Blood loss showed no significant difference between VATs and RATs (mean difference [MD], -33.00; 95% CI, -85.51 to 22.89; p = 0.07). Conversion to thoracotomy was comparable (MD, 0.69; 95% CI, 0.37 to 1.28; p = 0.24). Hospital stay did not differ significantly (MD, 0.13; 95% CI, -0.58 to 0.84; p = 0.74). Operation time remained similar (MD, -4.63; 95% CI, -10.93 to 1.67; p = 0.15). The VATS procedure enabled significantly greater lymph node dissection (MD, 1.83; 95% CI, 0.16 to 3.49; p = 0.03). Chest drainage duration showed no difference (MD, 0.00; 95% CI, -0.27 to 0.27; p = 1.00).

CONCLUSION: In NSCLC, RATS and VATS yield comparable outcomes, with RATS potentially reducing blood loss and VATS allowing more lymph node dissection. Further high-quality RCTs are needed to clarify the optimal approach for NSCLC.

PMID:41369812 | DOI:10.1245/s10434-025-18862-w

Environ Monit Assess. 2025 Dec 10;198(1):34. doi: 10.1007/s10661-025-14809-6.

ABSTRACT

This study examined nutrient and pollutant accumulation in edible crops grown on a solid waste dumpsite in Mbale, Uganda, to highlight the nutritional benefits and toxicological risks from this food production. Previously, the dumpsite was categorized into three sampling zones based on topography, waste type, human activity, and other environmental conditions. A total of 31 crop types were sampled, yielding 192 edible plant parts, which were then analyzed for 20 essential and non-essential elements. Leafy vegetables, root tubers, fruits, cereals, seeds, and lemongrass exhibited high levels of essential macronutrients and crucial micronutrients, in addition to toxic elements. The concentrations of aluminum (Al), nickel (Ni), chromium (Cr), lead (Pb), mercury (Hg), and certain nutrients exceeded the allowable consumption limits as per the FAO and WHO guidelines, in Table 2. Crops significantly, contained either: (i) high concentrations of potassium (K), calcium (Ca), magnesium (Mg), and phosphorus (P), (ii) high K levels, (iii) increased sodium (Na) content, (iv) mainly Al and iron (Fe) or (v) an average concentration of manganese (Mn), copper (Cu), barium (Ba), and zinc (Zn). The accumulation varied based on crop species, plant parts, sample site location, and site-specific conditions, with leafy crops exhibiting between 50 and 75% higher elemental content compared to other types or parts. Significant differences were observed in Cr, Zn, and Ba concentrations across the three zones, with higher Cr found at the dump centre, Zn at the slope, and Ba at the riverbank. Regardless, consumers of Mbale dumpsite crops are exposed to both beneficial and toxic elements. These findings should be simplified into local languages and shared through educational materials and community outreach. This will raise public awareness, promote safer agricultural practices, and guide policy interventions to protect food safety and public health in communities that rely on crops grown on waste dumpsites.

PMID:41369804 | DOI:10.1007/s10661-025-14809-6

Eur J Orthop Surg Traumatol. 2025 Dec 10;36(1):45. doi: 10.1007/s00590-025-04596-8.

ABSTRACT

PURPOSE: Horseback riding (HBR) presents a significant risk of injury, particularly fractures. While popular in Sweden, data on HBR-related fractures remains limited in its specificity regarding demographics and injury patterns. This study sought to delineate the distribution of HBR-related fractures in Sweden, considering sex, age, fracture site, injury mechanism, and seasonal trends.

METHODS: We conducted a retrospective observational study using Swedish Fracture Register (SFR) data from 2015 to 2022. All HBR-related fractures were included. Data on sex, age, fracture site, injury energy level, and injury date were analysed using descriptive statistics.

RESULTS: Some 5,453 fractures in 5,001 patients were included. Females accounted for 93% of cases. Upper extremity fractures were most prevalent, with wrist fractures representing the most common location (21%). Fractures of the forearm and humerus were most common among children, in contrast to adults, who presented with a wider array of fractures. High-energy mechanisms constituted 30% of fractures and were more often associated with injuries to the spine, pelvis, and femur. Fracture incidence peaked in May and September, coinciding with increased riding activity. Fracture incidence showed an age-dependent increase, exhibiting a bimodal distribution among females, with peaks near 10 and 55 years of age.

CONCLUSION: In the Swedish context, HBR-related fractures predominantly affect the upper extremities and occur most frequently in female riders, with distinct seasonal and age-related patterns. Although females account for most cases, the absence of detailed demographic data on the active riding population limits the ability to calculate precise incidence rates.

PMID:41369799 | DOI:10.1007/s00590-025-04596-8

J Am Heart Assoc. 2025 Dec 10:e042785. doi: 10.1161/JAHA.125.042785. Online ahead of print.

ABSTRACT

BACKGROUND: Transcatheter edge-to-edge repair of the mitral valve (M-TEER) has demonstrated promising outcomes for patients with mitral regurgitation who are unsuitable for surgical intervention. However, the clinical implications of M-TEER in patients requiring mechanical circulatory support (MCS) for unstable hemodynamics remains unclear. This study aimed to evaluate the outcomes of MCS-assisted M-TEER in critically ill patients.

METHODS: Consecutive patients who underwent M-TEER for significant mitral regurgitation were identified from the Japanese multicenter OCEAN-Mitral (Optimized Catheter Valvular Intervention-Mitral) registry. All-cause death was compared between the cohort undergoing MCS-assisted M-TEER and a virtual control group receiving MCS alone without undergoing M-TEER, estimated using the Seattle Heart Failure Model. Additionally, logistic regression analysis was conducted to identify factors associated with in-hospital death following MCS-assisted M-TEER.

RESULTS: A total of 3764 patients were included in the analysis (mean age, 81 years; 54.9% men; 70.1% with secondary mitral regurgitation), of whom 105 underwent MCS-assisted M-TEER. Procedure success exceeded 95%, irrespective of MCS use. The 1-year mortality rate in the MCS-assisted M-TEER group was lower than the estimated mortality rate in the virtual control group (43.2% versus 69.5%). Factors associated with in-hospital death included frailty, inflammation, and hypoalbuminemia. In a landmark analysis starting from discharge date, there was no statistically significant difference in cardiovascular death or heart failure hospitalization between the groups with or without the use of MCS (hazard ratio, 1.45 [95% CI, 0.93-2.26]; P=0.10).

CONCLUSIONS: MCS-assisted M-TEER appears to be a viable and effective therapeutic strategy for carefully selected patients with significant mitral regurgitation and unstable hemodynamics.

REGISTRATION: URL: https://www.umin.ac.jp/ctr/; Unique identifier: UMIN-ID: UMIN000023653.

PMID:41368845 | DOI:10.1161/JAHA.125.042785

Food Addit Contam Part B Surveill. 2025 Dec 9:1-7. doi: 10.1080/19393210.2025.2576912. Online ahead of print.

ABSTRACT

Animal feedstuffs are prone to fungal contamination due to their protein content and high moisture levels at harvest and storage, often leading to mycotoxin production. This study investigated the occurrence of aflatoxins in animal feed and the feed ingredients cottonseed cake and maize oil cake, collected from livestock farms in Okara, Punjab, Pakistan (2021-2022). Aflatoxins were extracted with an acetonitrile – water mixture, purified with Mycosep columns, quantified by HPLC with fluorescence detection and confirmed by mass spectrometry. Aflatoxins were detected in 74% of the animal feed samples, in 87% of the cottonseed cake samples and in 63% of the maize oil cake samples. Notably, 33% of animal feed and 70% of oilseed cake samples exceeded the maximum limit of 20 µg/kg. These findings revealed a high level of aflatoxin contamination, particularly in oilseed cakes, with statistically significant results (p < 0.05), highlighting potential animal health risks.

PMID:41368818 | DOI:10.1080/19393210.2025.2576912

Am J Epidemiol. 2025 Dec 10:kwaf274. doi: 10.1093/aje/kwaf274. Online ahead of print.

ABSTRACT

The COVID-19 pandemic highlighted deep-rooted health inequities globally, with marginalized populations showing disproportionate disease burden. We employed Serfling regression models and multivariable analyses to estimate excess mortality across geographic, demographic, and poverty groups from 2020-2022 in Chile. Elderly populations (80+ years) experienced the highest excess mortality (267.35 per 10,000 population), more than eight times higher than those under 80 years (30.80 per 10,000 population). Multivariable linear regression models showed both Indigenous proportion (coefficient=53.66, p<0.001) and elderly population proportion (coefficient=5.68, p<0.01) as the strong predictors of comuna level excess mortality. Poverty correlated significantly with excess mortality (r=0.23, p<0.001) but this association weakened after adjustment for other covariates in multivariable models. Excess mortality peaked in 2021 rather than in 2020 for most groups, with males initially experiencing higher rates during early pandemic waves. Spatial analyses revealed statistically significant clustering (Moran’s I=0.119, p<0.001) with identifiable hotspots in northern Chile and parts of the south. These findings indicated persistent mortality disparities by age and Indigenous status, independent of poverty, and highlight the urgent need for equity-focused pandemic preparedness. An effective pandemic response should integrate biomedical measures, such as vaccination, with culturally grounded strategies that address structural barriers and the broader social determinants of health.

PMID:41368772 | DOI:10.1093/aje/kwaf274

J Prosthodont. 2025 Dec 10. doi: 10.1111/jopr.70076. Online ahead of print.

ABSTRACT

PURPOSE: To investigate the relevant factors affecting the accuracy of the autonomous dental implant robotic system (ADIR)-assisted implant surgery, providing the foundation for the clinical application of ADIR.

MATERIALS AND METHODS: This retrospective study involved 85 patients with 138 implants who underwent ADIR-assisted implant surgery between March 2022 and May 2024. Drilling plans and virtual preoperative implant placement were designed preoperatively. Cone-beam computed tomography (CBCT) was used immediately after surgery to assess the linear and angular deviations in two-dimensional (2D) and three-dimensional (3D) space between planned and actual implants. Generalized estimation equations (GEE) were applied to analyze the relevant factors affecting the accuracy of ADIR-assisted implant surgery.

RESULTS: The GEE showed a significant correlation between relevant factors (implant position and length) and the linear deviations at both the 2D platform and apex in ADIR-assisted implant surgery (p < 0.05). The platform and apex are more deviated linearly in the anterior region than in the posterior region. Compared to 8 mm implants, 12 and 16 mm implants exhibited larger linear deviation. Sex and age were relevant factors influencing implant 3D positional deviation. Females and patients over 40 years of age showed increased 3D linear deviation of the platform (p < 0.05), but there was no statistical difference in angular deviation. Implant shape, diameter, bone classification, side of arch, guided bone regeneration (GBR), and flap procedure had statistically nonsignificant effects on ADIR-assisted implant surgical accuracy.

CONCLUSIONS: The relevant factors (implant length, implant position, age, and sex of the patient) may affect the accuracy of ADIR-assisted implant surgery. This study is of clinical interest and helps surgeons make clinical decisions to improve the precision of implant surgery.

PMID:41368769 | DOI:10.1111/jopr.70076

Nurs Adm Q. 2025 Dec 10. doi: 10.1097/CIN.0000000000001397. Online ahead of print.

ABSTRACT

Although the significance of big data and data science in predicting health outcomes and identifying causal factors is widely recognized, their application in health disparities research remains limited. Understanding health disparities in the visually impaired population requires examining their health behavior patterns and health literacy levels, which can longitudinally impact their health and well-being. In prior research, one of the authors of the study conducted an online survey with 2718 participants using 5 validated self-reported questionnaires, such as Health Promoting Lifestyle Profile II, Health Literacy Questionnaire, eHealth Literacy Questionnaire, General Self-Efficacy, and Center for Epidemiological Studies Depression. Analysis of the online survey data demonstrated that individuals with blindness exhibited significantly higher levels of health-promoting behaviors, health literacy, and eHealth literacy compared to those with moderate and severe low vision. In this study, the research team developed an R Shiny web application as a follow-up to the online survey to disseminate its findings reproducibly and interactively. The R Shiny web application is expected to facilitate reproducible as well as interactive data analysis and sharing more efficiently than traditional methods, such as appendices or Supplementary Materials, Supplemental Digital Content 2, http://links.lww.com/CIN/A463 in academic journals. Extending the research cycle with open datasets and reproducible data analysis can deepen our understanding of health disparities and foster greater collaboration among researchers with similar interests.

PMID:41368760 | DOI:10.1097/CIN.0000000000001397

J Contemp Dent Pract. 2025 Nov 1;26(11):1092-1095. doi: 10.5005/jp-journals-10024-3962.

ABSTRACT

AIM: The current study aimed to evaluate the penetration depth and sealing ability of three different endodontic sealers.

MATERIALS AND METHODS: A total of 60 extracted human mandibular premolars for orthodontic reasons with a single canal were divided randomly into three groups of 20 samples-Group I: Tubli-Seal [zinc oxide-eugenol (ZOE)-based sealer], group II: Endosequence [bioceramic (BC) sealer], group III: AH Plus (epoxy resin-based sealer). At the coronal, middle, and apical thirds, three transversal segments of 1 mm thickness were created using a low-speed precision IsoMet cutter running at 200 rpm while being continuously cooled by water. Following a gold-palladium sputtered coating, these slices were seen at ×1000 magnification using a scanning electron microscope (SEM). The gaps in between the sealer and the canal wall were measured by analyzing images. Data was recorded and statistically analyzed.

RESULTS: The maximum sealing ability was found in Endosequence BC sealer i.e., 3.96 ± 0.16 followed by AH Plus sealer i.e., 2.32 ± 0.08 and Tubli-Seal sealer i.e., 1.04 ± 0.10. On intergroup comparison at coronal third; a statistically significant difference was obtained between Tubli-Seal sealer vs Endosequence BC sealer and Endosequence BC sealer vs AH Plus sealer. At middle and apical third, significant difference was obtained between Tubli-Seal sealer vs Endosequence BC sealer, Tubli-Seal sealer vs AH Plus sealer and Endosequence BC sealer vs AH Plus sealer (p < 0.001).

CONCLUSION: On conclusion, according to the current investigation, Endosequence BC sealer had the best sealing ability, followed by AH Plus sealer and Tubli-Seal sealer.

CLINICAL SIGNIFICANCE: The effectiveness of endodontic therapy depends on proper root canal closure. There are many endodontic sealers available; finding easy-to-use and effective items is crucial to providing quality patient care. When a root canal system is fully sealed, oral pathogens cannot colonize and re-infect the root and periapical tissues. How to cite this article: Mohan D, Lahiri B, Sreenivasalu PKP, et al. Assessment of the Sealing Ability and Penetration Depth of Different Endodontic Sealers: A Scanning Electron Microscope Study. J Contemp Dent Pract 2025;26(11):1092-1095.

PMID:41368750 | DOI:10.5005/jp-journals-10024-3962