Category: Nevin Manimala

Mol Genet Metab. 2026 Apr 2;148(2):110112. doi: 10.1016/j.ymgme.2026.110112. Online ahead of print.

ABSTRACT

INTRODUCTION AND OBJECTIVE: Mutations in the GBA1 gene, particularly the severe L444P variant, are among the strongest known genetic risk factors for Parkinson’s disease (PD). Prior meta-analyses published before 2018 included fewer studies and often pooled multiple mutations, prompting this updated, mutation-specific systematic review and meta-analysis.

METHODS: Standard systematic review and meta-analytic methods were used, including predefined eligibility criteria, literature search, and pooled analysis of odds ratios (ORs) for PD in GBA1 L444P carriers versus non-carriers.

RESULTS: We included 51 studies comprising 33,752 PD patients and 34,101 controls across multiple continents. In random-effects meta-analysis, heterozygous GBA1 L444P carriers had markedly higher odds of PD than non-carriers (pooled OR 9.19, 95% CI 6.94-12.16), with similar estimates across sensitivity and leave-one-out analyses. Cumulative meta-analysis showed early variability but stable pooled ORs around 7-9 from 2008 to 2010 onward. Ancestry-specific pooled ORs were consistently elevated across populations, with overlapping confidence intervals and no significant subgroup differences. Funnel and Galbraith plots and Egger’s test showed no evidence of substantial small-study effects or publication bias.

CONCLUSIONS: This updated synthesis demonstrates that heterozygous GBA1 L444P carriage confers a consistently large increase in PD risk across populations, with robust pooled estimates across multiple sensitivity analyses. Although overall statistical heterogeneity was negligible, wide confidence intervals in several ancestry subgroups highlight imprecision due to sparse data and internal heterogeneity, underscoring the need for broader and more equitable genetic testing, improved risk estimation in underrepresented populations, and targeted research on severe GBA1 variants to inform genetic counseling and PD risk discussions.

REGISTRATION: PROSPERO Registration Number: CRD420251182022. Registration details available at the PROSPERO database.

PMID:41936135 | DOI:10.1016/j.ymgme.2026.110112

GM Crops Food. 2026 Dec 31;17(1):2653897. doi: 10.1080/21645698.2026.2653897. Epub 2026 Apr 5.

ABSTRACT

GMO testing laboratories operating within the current European Union (EU) regulatory framework governing the presence of genetically modified organisms (GMOs) in food and feed (EC Reg. 1829/2003 and EC Reg. 1830/2003) perform a stepwise workflow from DNA extraction to quantification of genetically modified events. A very valuable intermediate step in guiding and optimizing this workflow is the screening phase, where any positives require the laboratory to proceed to the subsequent identification and quantification steps. Very often, however, samples with low GM content result positive at screening but then the GM component is not quantifiable, wasting time and resources. In order to overcome this issue, in this study a statistical framework was developed to predict the presence of soybean GM events based on the difference between the quantification cycle (Cq, also known as threshold cycle (Ct) of the Real time PCR technique) values observed from screening elements as cauliflower mosaic virus 35S promoter (P35S), the nopaline synthase terminator (T-nos), the 5-enolpyruvylshikimate -3-phosphate synthase gene (CP4 epsps) and lectin reference gene (Lec) (ΔCq values). The feasibility of this approach was successfully in-house verified on real life and spiked samples. This approach can be seen as a proof of concept to suggest how to optimize, on a statistical basis, the workflow of GMO testing laboratories that need to evaluate sample compliance with quantitative tests.

PMID:41936134 | DOI:10.1080/21645698.2026.2653897

Nurs Open. 2026 Apr;13(4):e70516. doi: 10.1002/nop2.70516.

ABSTRACT

AIM: To assess the nursing and midwifery students’ knowledge and practice regarding fluid resuscitation during the acute phase of burn management.

DESIGN: A cross-sectional, descriptive design was employed.

METHODS: The data were collected using an online-administered questionnaire at a governmental university among nursing and midwifery students, who were selected purposively. The instrument consisted of three sections. The first section covered participants’ characteristics, including age, sex, marital status, academic program, level of study, grade point average (GPA) and residency. The second section is self-reported and composed of 10 questions to assess the students’ knowledge domains, and the third section is also self-reported and composed of 11 questions to assess participants’ practice regarding practice; each question received a binary response based on whether or not students completed a specific practice.

RESULTS: The study of midwifery and nursing students reported that they had an average knowledge (73.58%) of fluid monitoring, with 96% comprehending fluid balance, but only 28.4% being able to calculate fluids using the Parkland formula. In practice, participants demonstrated a high level of compliance (94.89%) with documentation of fluid administration; however, adherence was notably lower (85.7%) for the application of the rule of nines during assessment.

PATIENT CONTRIBUTION: Midwives and nurses draw clinical judgements based on their academic and professional experience, as well as scientific facts. Theoretical knowledge can help support and improve health practices. A strong intellectual background is essential for providing appropriate care throughout the acute phase of burn management. Future research could incorporate direct observation methods to validate self-reported findings and assess the translation of theoretical knowledge into clinical practice.

PMID:41936122 | DOI:10.1002/nop2.70516

Nurs Open. 2026 Apr;13(4):e70523. doi: 10.1002/nop2.70523.

ABSTRACT

AIM: This study was conducted to examine the effect of nursing practice environment individual and occupational characteristics on nurses’ attitudes towards medical error.

DESIGN: Cross-sectional study.

METHODS: The questionnaire was used for data collection in a tertiary university hospital from 4 March to 30 April 2022. The questionnaire included the “Information Form”, the “Practice Environment Scale-Nursing Work Index (PES-NWI)” and the “Medical Error Attitude Scale”. The population of this study included 1329 nurses. A total of 355 nurses who voluntarily completed the questionnaires constituted the study sample. Convenience sampling was used to select participants. Since the study was conducted at a single hospital, the survey was emailed to all nurses at the institution to ensure broad accessibility. Pearson correlation analysis was used to assess the relationships between continuous variables. Multiple linear regression analysis was employed to examine factors influencing the scores on the Medical Error Attitude Scale. Ethical approval was obtained from the institutional ethics committee, and informed consent was obtained from all participants. The findings were reported following the STROBE guidelines.

RESULTS: The mean score of the PES-NWI was 2.60 ± 0.45 and the mean score of the Medical Error Attitude Scale was (3.50 ± 0.39). Regression analysis was performed to identify factors influencing nurses’ attitudes towards medical errors. According to the results of the multiple regression analysis, age (p < 0.001), duration of experience in the institution (p < 0.001), educational status (p < 0.001), general satisfaction with patient safety in the unit (p < 0.05) and two subdimensions of the PES-NWI-“Nurse manager ability, leadership and support of nurses” (p < 0.05) and “Staffing and resource adequacy” (p < 0.05)-were significant predictors. These six variables collectively explained 12.8% of the total variance in the Medical Error Attitude Scale score (R² = 12.8%, F = 7.454, p < 0.001).

CONCLUSIONS: The study concluded that both the nursing practice environment and certain individual and occupational characteristics of nurses impact their attitudes towards medical errors. In line with this study’s results, we recommend that institutional managers and nurse managers make necessary arrangements to improve nurses’ attitudes towards medical errors, as well as to develop institutional strategies designed to prevent medical errors in their units/institutions, thereby improving patient safety and quality of care.

NO PATIENT OR PUBLIC CONTRIBUTION: The participants of this study were nurses and this contribution has been explained in the data collection section. There was no patient contribution in this study.

PMID:41936116 | DOI:10.1002/nop2.70523

Pharm Nanotechnol. 2026 Mar 25. doi: 10.2174/0122117385394646251111112131. Online ahead of print.

ABSTRACT

Wounds associated with diabetes mellitus are among the most serious complications, with the potential to progress to cell necrosis and, in severe cases, necessitate amputation. Current statistics indicate that diabetic wounds affect approximately 15% of patients with diabetes, and 20% of these cases lead to limb amputation. Conventional therapies often prove ineffective due to molecular and structural alterations in the injured tissue, highlighting the need for innovative delivery methods to enhance treatment efficacy. Recent research has focused on developing advanced materials for wound management, particularly through phyto-nanotechnology. Topical applications of nanoscaffolds and nanofibers have shown promising outcomes in promoting wound healing. Nano-sized particles facilitate smooth progression through the healing phases by enhancing cellular and molecular interactions. Among these, silver nanoparticles (AgNPs) have attracted attention for diabetic wound treatment due to their potent antibacterial and anti-inflammatory properties. AgNPs also stimulate cellular mechanisms that support the repair of chronic wounds; however, potential toxicities remain a concern. This review examines the role and mechanisms of biogenic AgNPs in diabetic wound management, with a focus on their efficacy in wound dressings. Additionally, it examines marketed AgNP formulations for wound care, highlights reported toxicity issues, and discusses clinical trials and future prospects for their application in wound healing.

PMID:41936080 | DOI:10.2174/0122117385394646251111112131

Alcohol Alcohol. 2026 Mar 17;61(3):agag020. doi: 10.1093/alcalc/agag020.

ABSTRACT

BACKGROUND AND AIMS: The present study aimed to: (i) compare a patient group with solely alcohol use disorder (AUD) to a group with poly-substance use disorder (poly-SUD) regarding sociodemographic background, morbidity, mortality, and the prevalence of the A1 allele of the Taq1A polymorphism. (ii) Investigate whether gender, age, poly-SUD, and the prevalence of the A1 allele or interactions among these factors, are associated with mortality risk over an 18-year follow-up period.

METHODS: This study comprised 360 individuals treated for severe alcohol withdrawal symptoms in 1997 at a treatment unit in Sweden. Genotyping was performed during their hospital stay, and participants were followed annually for up to 18 years using data from Swedish registers.

RESULTS: Fifty-three percent of the participants had died over the 18 year period. Poly-SUD patients exhibited higher rates of psychiatric disorders, gastrointestinal diseases, and intoxication as the primary diagnosis. Patients with AUD exhibited a higher prevalence of cardiac diseases. Traumatic causes of death were more prevalent in the poly-SUD group, whereas somatic diseases were more common among individuals with AUD. Male sex and age were the strongest predictors of premature death among individuals with AUD. The A1 allele of the Taq1A polymorphism showed a borderline association with an increased hazard of death.

CONCLUSIONS: Male sex and age are the strongest predictors of premature death. Patients with poly-SUD may represent a distinct subgroup with different comorbidities and causes of death. To determine whether there is a genetic vulnerability as indicated by the findings, research using larger samples with sufficient statistical power is needed.

PMID:41936048 | DOI:10.1093/alcalc/agag020

Aging Ment Health. 2026 Apr 5:1-12. doi: 10.1080/13607863.2026.2643895. Online ahead of print.

ABSTRACT

OBJECTIVES: Subjective wellbeing is a self-defined appraisal of happiness that includes judgments of life satisfaction and overall affect. Health-related quality of life is an appraisal of wellbeing focused on one’s health status. Factors influencing these different aspects of wellbeing in old age remain unclear, with particular uncertainty around cognition. This study examined these relationships through assessments over 12 years.

METHOD: One-thousand-and-thirty-seven people without dementia were recruited as part of a population-based cohort study. Participants completed a neuropsychological battery, medical history, and measures of life satisfaction, positive affect, health-related quality of life, depression, and anxiety biennially over 12 years. Participants also completed personality measures and informants completed measures of function. Linear mixed models were used to examine whether cognition and other variables were associated with wellbeing measures.

RESULTS: Life satisfaction, positive affect, and health-related quality of life were moderately correlated at baseline. Cognition had a small, but statistically significant longitudinal association with each of these outcomes after adjusting for potential confounders. Physical health, function, personality, and anxiety also predicted wellbeing measures.

CONCLUSION: Cognition is associated with subjective wellbeing and health-related quality of life in older people. Physical health, function, personality, and anxiety are also important predictors of wellbeing in old age.

PMID:41936036 | DOI:10.1080/13607863.2026.2643895

Orv Hetil. 2026 Apr 5;167(14):547-555. doi: 10.1556/650.2026.33516. Print 2026 Apr 5.

ABSTRACT

INTRODUCTION: Greater emphasis must be placed on monitoring mental health in the healthcare sector in order to ensure the standard and quality of patient care, taking into account psychosocial and socioeconomic factors. The mental state of healthcare professionals plays a major role in patient safety.

OBJECTIVE: To assess and compare the general well-being, depression, and burnout levels of healthcare professionals in a sample from Békés county (Hungary) and Hargita county (Romania).

METHOD: We conducted our cross-sectional questionnaire survey among nurses in Békés and Hargita counties using the WHO Well-Being Questionnaire, the Beck Depression Questionnaire, and the Maslach Burnout Inventory. We analyzed our responses using chi-square and t-tests, nonparametric measurement tools, and Spearman’s correlation analysis (p<0.05) with the help of SPSS 30.0 software. The study included 117 Hungarian and 109 Romanian professionals (n = 226).

RESULTS: Based on the results, we found that there was no significant correlation between general well-being (p = 0.772), depressive symptoms (p = 0.338), and burnout in the dimensions of depersonalization (p = 0.090) and personal efficacy (p = 0.586) in the two groups studied. Among Hungarian professionals, emotional exhaustion is significantly higher (p<0.05) and their subjective assessment of their general and mental health is worse than that of their Romanian colleagues (p<0.05).

DISCUSSION: We detected a risk of depression and burnout in both target groups. The study highlights the need for intervention in the mental health of Hungarian healthcare professionals and the justification for further research into the causal factors.

CONCLUSION: The workload of healthcare professionals remains high in the post-pandemic period, and the domestic situation is further complicated by the fact that chronic diseases are more prevalent among Hungarian nurses, which has a significant impact on our mental health indicators. Improving mental health would require reforming the sector, increasing the frequency of continuing education, and integrating practical mental hygiene methods. Orv Hetil. 2026; 167(14): 547-555.

PMID:41936032 | DOI:10.1556/650.2026.33516

Clin Nurs Res. 2026 Apr 5:10547738261429348. doi: 10.1177/10547738261429348. Online ahead of print.

ABSTRACT

Critically ill patients frequently require intrahospital transport (IHT) to diagnostic exams or procedures and must remain connected to life-sustaining medical lines. During IHT, the risk of tangled medical lines is unavoidable. The idea for a novel medical line securement device was inspired by a patient transport nurse due to frustrations and workflow inefficiencies managing tangled medical lines. The study aim was to explore the correlation between the utilization of a single-use securement device to manual or accidental disconnections of medical lines during IHT, and nurses’ perception of efficiency, compared to the current practice of no utilization of a securement device. In 2019, a randomized, descriptive, correlational study was conducted on 142 progressive and intensive care hospitalized patients. Study coordinators measured the number of times either IV lines or monitor cords were accidentally or manually disconnected to detangle during or after transportation and the perception of transport efficiency. Results revealed fewer manual disconnections among intervention group compared to the control. Nurses’ perception regarding the efficiency between the intervention and control group was statistically significant when the device was utilized to secure the lines and cords during transport. Nurses reported much higher satisfaction rates of transport efficiency when the device was used. This finding showed the device’s utility for improving nurse satisfaction during IHT of critically ill patients. Utilization of the medical line securement device may be generalizable across other healthcare settings and disciplines where the complexity of medical lines may exist.

PMID:41936022 | DOI:10.1177/10547738261429348

Arch Gynecol Obstet. 2026 Apr 5;313(1):153. doi: 10.1007/s00404-026-08386-3.

ABSTRACT

BACKGROUND: This study analyzed medical records of patients with climacteric syndrome at a Korean medicine hospital and investigated the interrelationships among their symptoms using network analysis.

METHODS: A retrospective chart review was conducted on 81 women (aged 45-60 years) who visited a women’s health clinic between 1 January 2020 and 31 December 2021. Network analysis was performed on unstructured text from first-visit medical records to identify symptom patterns and compute centrality indices.

RESULTS: The most common symptoms were ‘pain’, ‘hot flush’, ‘facial blushing’, ‘fatigue’, and ‘sleep disorder’. Among them, pain and hot flush showed high node strength, betweenness centrality, and closeness centrality, which indicated their central role in symptom co-occurrence. Although there were no statistically significant differences in the frequency of symptoms between the premenopausal and postmenopausal groups, variations in symptom centrality and modular analysis results before and after menopause were noted, emphasizing the importance of detailed symptom analysis in clinical settings.

CONCLUSIONS: By analyzing medical records of patients with climacteric syndrome, this study clarified symptom patterns in menopausal women, providing data for targeted and stage-specific treatments.

PMID:41935990 | DOI:10.1007/s00404-026-08386-3