Category: Statistics

Virchows Arch. 2025 May 5. doi: 10.1007/s00428-025-04104-7. Online ahead of print.

ABSTRACT

Tubo-ovarian high-grade serous carcinoma (HGSC) with proficient homologous recombination (HR) DNA repair (HRP) accounts for approximately 50% of cases and is associated with platinum-resistance and poor prognosis. We hypothesize that the acquisition of hybrid phenotypes displaying both epithelial and mesenchymal (E/M) features may be involved in the malignant transformation and tumour dissemination in this subgroup. Therefore, we analysed, by digital pathology, the expression and prognostic significance of 3 classic cadherins (E-cadherin, epithelial marker; N-cadherin, mesenchymal marker; and P-cadherin, candidate marker of hybrid E/M) in 577 formalin-fixed paraffin-embedded human samples representing the putative stepwise serous carcinogenesis in the Fallopian tube epithelium (FTE). We observed a non-canonical N-to-P-cadherin switch along the carcinogenic progression, with a statistically significant overexpression of P-cadherin in pre-malignant and malignant samples, compared to the control FTE. Interestingly, this overexpression was most pronounced in precursor lesions and HGSC cells from malignant ascites. Tumours with high P-cadherin expression were significantly associated with worse overall survival, especially in the subgroup without BRCA1/2 mutations. Transient P-cadherin knock-down resulted in in vitro significant reduction of functional hybrid E/M hallmarks, namely decreased anoikis resistance, reduced collective migration and invasion in a representative platinum-resistant HRP cell line. Taken together, our results suggest that P-cadherin overexpression is an early event in the serous carcinogenesis and may be involved in hybrid E/M activation in HRP-HGSC, further supporting this adhesion molecule as a promising biomarker for this poor prognostic subgroup.

PMID:40320493 | DOI:10.1007/s00428-025-04104-7

Exp Brain Res. 2025 May 4;243(6):136. doi: 10.1007/s00221-025-07042-7.

ABSTRACT

The neural processes underlying gait retraining interventions that combine stepping practice with electrical stimulation are poorly understood. The purpose of this study was to compare acute changes in corticomotor excitability measured by motor evoked potential (MEP) amplitude, monosynaptic stretch reflex excitability measured by H/M ratio, and propulsive force generation during gait measured by anterior ground reaction forces (AGRF), induced by 30-minutes of fast walking (Fast) and 30-minutes of fast walking with functional electrical stimulation of the ankle plantarflexors and dorsiflexors (FastFES) in 14 able-bodied young adults. Our results showed that FastFES, but not Fast elicited a significant acute decrease in tibialis anterior MEP amplitude (p = .01). Furthermore, the practice-induced acute decrease in tibialis anterior MEP amplitudes was significantly larger for FastFES than Fast (p = .04). FastFES also elicited a statistically significant increase in the AGRF in the tested limb (p = .01), which was significantly larger than the change induced by Fast (p = .04). Additionally, baseline soleus MEP amplitude was positively correlated with within-session change in AGRF (p = .04, r² = 0.16). Acute decrease in tibialis anterior MEP amplitude for the FastFES condition relative to the Fast condition may be caused by fatigue, while the greater increase in AGRF for the FastFES condition signify that stepping practice facilitated alterations of gait patterns. More research is needed to confirm neural mechanisms and investigate the acute as well as long-term effects of Fast and FastFES on clinical populations.

PMID:40320480 | DOI:10.1007/s00221-025-07042-7

Clin Oral Investig. 2025 May 5;29(5):284. doi: 10.1007/s00784-025-06358-6.

ABSTRACT

OBJECTIVES: This study aimed to evaluate the clinical success and long-term survival of glass hybrid restorations in permanent first molars affected by molar incisor hypomineralization (MIH) following selective caries removal (SCR) over a six-year follow-up period.

MATERIALS AND METHODS: This prospective cohort study included a total of 134 MIH-affected molars in 58 children (mean age 8.94 ± 1.41 years) restored with glass hybrid materials after SCR. Clinical outcomes were assessed at baseline and at 6, 12, 18, 24, and 72 months using modified USPHS criteria. Kaplan-Meier survival analysis and Cox proportional hazards regression with robust standard errors were conducted to evaluate restoration survival and identify potential predictors.

RESULTS: The overall estimated mean survival time of restorations was 59.82 ± 1.50 months. The survival probabilities for mild and severe lesions at six-year were 24.3% and 11.1%, respectively. Multivariate Cox proportional hazards regression with robust standard errors indicated that lesion severity, medium lesion extension, and large lesion extension had a statistically significant impact on restoration survival (p < 0.001 for all). While survival was satisfactory up to 2-3 years, a marked decline was observed over time, particularly in severely affected molars.

CONCLUSIONS: Glass hybrid restorations demonstrated acceptable clinical performance in MIH-affected molars in the short to medium term but showed significant limitations in long-term survival.

CLINICAL RELEVANCE: The need for advancements in restorative materials tailored to the structural challenges of MIH. Glass hybrid materials remain a viable option for managing MIH-affected molars, particularly in young patients where minimally invasive approaches are prioritized.

PMID:40320479 | DOI:10.1007/s00784-025-06358-6

World J Urol. 2025 May 4;43(1):264. doi: 10.1007/s00345-025-05521-8.

ABSTRACT

OBJECTIVE: This study aims to assess the current status of self-management ability in patients with neurogenic bladder resulting from traumatic spinal cord injury (TSCI) and to analyze the factors influencing this ability.

METHOD: A cross-sectional study was conducted from November 2023 to July 2024, a total of 305 patients with neurogenic bladder due to TSCI were recruited from over 30 medical institutions, including rehabilitation centers within tertiary general hospitals, secondary rehabilitation hospitals, and private rehabilitation facilities across Shandong Province, China. Face-to-face interviews were conducted using a general information questionnaire, the patient positive measurement scale, the social support rating scale, and the self-management ability scale. Pearson correlation analysis and multiple linear regression were employed for statistical analysis.

RESULTS: Among 305 patients with neurogenic bladder caused by TSCI, the score of positivemeasurement scale was 40.95 ± 8.26, the score of the social support scale was 38.12 ± 8.35, and the score of self-management ability scale was 118.19 ± 37.16. In univariate analysis, social support (β = 0.364, P < 0.001), positive degree (β = 0.505, P < 0.001) and bladder training (β = -0.349, P < 0.001) positively predicted self-management ability. Age was a negative predictor of self-management ability (β = -0.204, P < 0.001). In multiple linear regression analysis, social support (β = 0.194, P < 0.001), bladder training (β = -0.262, P < 0.001) and positive degree (β = 0.376, P < 0.001) were still positively correlated with self-management ability.

CONCLUSION: Self-management ability in patients with neurogenic bladder secondary to TSCI is moderate, and patient activation is low. The establishment of an effective social support system, the implementation of bladder training and the improvement of patients positive degree can be used as an entry point to improve their self-management behavior ability, so as to reduce the patients’ dependence on others care and prepare for discharge and return to society.

CLINICAL TRIAL NUMBER: Not applicable.

PMID:40320459 | DOI:10.1007/s00345-025-05521-8

Sci Rep. 2025 May 4;15(1):15586. doi: 10.1038/s41598-025-99034-4.

ABSTRACT

Plastic materials are widely used because of their strength, light weight, durability, and environmental resistance. However, their decomposition rates are significantly slower than their typical lifespans. The rapid and continuous increase in plastic consumption has caused severe environmental impacts due to the accumulation of plastic waste. We identified potential polyhydroxyalkanoate (PHA)-degrading bacteria from marine environments capable of producing extracellular PHA depolymerases crucial for biodegrading PHAs. Marine debris was collected to screen poly [(R)-3-hydroxybutyric acid] (P(3HB))-degrading bacteria. Six isolates showed the ability to produce clear zones surrounding their colonies by degrading the bioplastic P(3HB). The isolate SS1-2, exhibiting the greatest degradation index of 1.44, was chosen for optimization through the statistical technique. The results indicated that NH₄Cl was the best nitrogen source for enzyme production, and the response surface methodology (RSM) suggested that the greatest P(3HB) depolymerase production could be achieved when the concentrations of substrate loading and NH₄Cl both set at 0.5%. Analysis of the 16S rRNA sequence of isolate SS1-2 revealed similarity to Pseudooceanicola antarcticus CGMCC 1.12662 (97.81% similarity). The findings of this study indicate the potential for further exploitation of this depolymerase in enzyme kinetics studies and its application in PHA degradation experiments.

PMID:40320445 | DOI:10.1038/s41598-025-99034-4

Sci Rep. 2025 May 4;15(1):15582. doi: 10.1038/s41598-025-99538-z.

ABSTRACT

Bacterial meningitis is a significant public health concern, with over 1.2 million cases reported globally each year. Rwanda is at increased risk of meningitis outbreaks due to its proximity to countries that lie in the meningitis belt. Rwanda has been conducting surveillance and recording meningitis outbreak cases across the country since 2012. We evaluated the meningitis surveillance system at Kibogora Level Two Teaching hospital, Nyamasheke district of Rwanda to assess whether the surveillance objectives were being met. The study was cross-sectional, using purposive sampling to select healthcare providers participating in the meningitis surveillance. Rwanda’s bacterial meningitis data from 2017 to 2021 was collected from clinical registers and Rwanda’s electronic integrated disease surveillance system (eIDSR) from Kibogora Level Two Teaching Hospital catchment area, Nyamasheke district, Rwanda. The study area was chosen because a meningitis outbreak was recorded in the area and its bordering country namely Democratic of Republic of Congo (DRC) prior to the current study period. Information on the participant’s demographics, occupation, training, professional experience, and their perception on the surveillance system were gathered using a structured questionnaire. Meningitis surveillance systems attributes including usefulness, acceptability, and flexibility were assessed and categorized as poor (< 50% score), reasonable (50-69%), good (70-90%), or excellent (> 90%) in reference to the study conducted on the evaluation of the meningitis surveillance system in Luanda Province, Angola in March 2017. Data collected from clinical registers and eIDSR were used to assess core functions of the meningitis surveillance system including accuracy in detection of cases, laboratory confirmation of cases, and availability of evaluation reports. Descriptive statistics were analyzed using Microsoft Office Excel. Thirty-one healthcare providers working on meningitis surveillance in the Kibogora Level Two Teaching Hospital were interviewed. During the period under evaluation, 48 suspected cases of meningitis were identified; 43 (90%) met the surveillance case definition, and only 10 (21%) were reported to eIDSR (completeness). Attributes such as flexibility scored good while stability and acceptability scored reasonable. Out of 48 suspected meningitis cases, only 2 (4%) samples were collected from patients and sent to the hospital laboratory for analysis. This study found a good knowledge level of the meningitis surveillance system among healthcare workers; however, the system’s core functions, such as notification rate and laboratory confirmation were found to have gaps. The notification rate could be improved by conducting regular refresher courses for healthcare workers supporting surveillance system. Moreover, MoH could enhance the implementation of a national policy requiring mandatory CSF sample testing to confirm pathogens for all suspected cases. Future studies should explore performance-based incentives to improve reporting completeness. Rwanda’s experience could provide insights for other low-resource settings facing similar surveillance challenges.

PMID:40320439 | DOI:10.1038/s41598-025-99538-z

Sci Rep. 2025 May 4;15(1):15603. doi: 10.1038/s41598-025-00466-9.

ABSTRACT

The intestinal microbiota, in terms of both composition and functioning, exerts a significant influence on the human body. Disturbed microbiota is a common occurrence in the context of numerous diseases. The available evidence increasingly points to a correlation between this condition and the development of prostate diseases, including benign prostatic hyperplasia and prostate cancer. 16 S sequencing of the V3-V4 region was performed and then evaluated alpha and beta diversity of the faeces microbiota of healthy (control group, N = 81) and BPH patients (study group, N = 76). The exploration of enterotypes involved the application of the Dirichlet-Multinomial model, executed for selecting community types. The study revealed no statistically significant difference in alpha diversity between the control group and the group of patients diagnosed with BPH. However, a significant difference was observed in beta diversity (Permanova test: F-value = 5.56, p-value < 0.001). The identification of enterotypes revealed significant differences between the healthy male cohort and those diagnosed with BPH (p = 0.035). In the cohort of men with BPH, the most prevalent was enterotype 3, characterized by a predominance of Blautia, Bacteroides, and Streptococcus. The occurrence of enterotype 3 was associated with an increased likelihood of BPH, exceeding threefold that of enterotype 1 (OR = 3.24). These findings suggest that alterations in the gut microbiota, particularly the presence of enterotype 3, may serve as a microbiological pattern associated with BPH.

PMID:40320423 | DOI:10.1038/s41598-025-00466-9

Sci Rep. 2025 May 4;15(1):15607. doi: 10.1038/s41598-025-00026-1.

ABSTRACT

To address the low repeatability and accuracy of traditional technologies for testing the human somatosensory system, this work presents a novel mechatronic testbed. The testbed allows for the delivery of mechanical and thermal stimuli with a high spatial resolution, enabling continuous or discrete stimulation with a small fixed area and in a single experimental session. The testbed was employed to identify the mechanical/thermal innocuous and painful thresholds and the human ability to distinguish the nature of a painful stimulus, on both the hand and the forearm of 12 healthy volunteers. The results demonstrated the capability of the developed testbed to produce a range of forces that can induce different sensations (touch or pain). We found a statistical difference between the innocuous and painful thresholds, regardless of the tested anatomical spot. In this paper, a small thermal stimulation tip was appositely selected to study the reaction to a focused thermal stimulus that has been poorly investigated so far. The results highlighted a statistically significant difference between the two stimulated sites for the cool sensation and the hot pain. Moreover, the painful recognition task was sped up by the use of the developed testbed, which allowed a more fair comparison among the applied stimuli, increasing the accuracy, repeatability, and consistency when compared to the state-of-the art.

PMID:40320402 | DOI:10.1038/s41598-025-00026-1

Sci Rep. 2025 May 4;15(1):15592. doi: 10.1038/s41598-025-00222-z.

ABSTRACT

Small cell lung cancer (SCLC) is a highly malignant lung cancer subtype with a dismal prognosis and limited treatment options. This study aimed to identify new prognostic molecular biomarkers for SCLC and explore their immune-related implications for treatment strategies. We analyzed 200 SCLC samples via whole-exome sequencing (WES) and 313 samples by targeted sequencing. A smoking-related SBS4 mutational signature was linked to poorer prognosis and lower tumor mutational burden (TMB), while the APOBEC-mediated SBS13 signature was associated with better prognosis and higher TMB. We identified a molecular subtype with the worst outcomes and lowest TMB in both cohorts. Among 38 high-frequency mutated genes associated with SCLC prognosis, only UNC13A mutations were beneficial. Patients with UNC13A mutations had favorable immune infiltration and tumor immunogenicity. Additionally, TP53 splice site mutations were related to the worst survival outcomes. In conclusion, we discovered new molecular biomarkers for SCLC prognosis. Our findings on their immunological characteristics offer insights for developing novel SCLC treatment strategies.

PMID:40320401 | DOI:10.1038/s41598-025-00222-z

Sci Rep. 2025 May 4;15(1):15613. doi: 10.1038/s41598-025-00288-9.

ABSTRACT

Good sleep quality is crucial for health and body equilibrium, particularly during pregnancy, where changes in sleep are influenced by mechanical and hormonal factors. Poor sleep can hinder daily activities and lead to adverse pregnancy outcomes. Data on sleep quality are scarce in low and middle-income countries such as Ethiopia. Hence, this study aimed to determine the magnitude of sleep quality and its associated factors among pregnant mothers in Bahir Dar City, northwest Ethiopia. A facility-based cross-sectional study was conducted with 367 randomly selected pregnant women. All pregnant women who attended public health facilities in Bahir Dar City from November 1st to December 30, 2022 were included except who were critically ill and aged less than 18 years. The data were collected through face-to-face interviews. Sleep quality was assessed by using the Pittsburgh Sleep Quality Index (PSQI). Stata v14 was used for data analysis. A binary logistic regression model was used to identify factors associated with poor sleep quality. Statistical significance was set at p < 0.05. The prevalence of poor sleep quality was 55.04%. In the multivariable analysis, older maternal age (AOR = 3.62), third trimester (AOR = 2.83), multigravidas (AOR = 2.55), low hemoglobin (AOR = 1.92), and coffee consumption (AOR = 2.19) were associated with poor sleep quality. More than half of pregnant women had poor sleep quality. Women aged ≥ 30 years, 3rd trimester, multigravidas, anemic women, and coffee consumption during pregnancy were factors associated with poor sleep quality. The concerned body should pay attention to improving Hgb level and iron/folate supplementation and reduce coffee intake in pregnant women to improve maternal sleep quality during pregnancy.

PMID:40320398 | DOI:10.1038/s41598-025-00288-9