Category: Statistics

Cell Mol Biol (Noisy-le-grand). 2023 Nov 15;69(11):69-75. doi: 10.14715/cmb/2023.69.11.12.

ABSTRACT

This study aims to investigate the Interleukin (IL)-6 rs1800795 and peroxisome proliferator-activated receptor alpha (PPARA) rs4253778 polymorphism distributions in the relatively faster and slower subgroups of national cross-country skiing athletes and to identify advantageous genotypes for endurance performance. IL-6 is an inflammatory mediator that is effective in muscle tissue hypertrophy, repair, and the immune system. On the other hand, PPARA transcription factor is a molecule associated with fatty acid, sugar metabolism and inflammation formation. Total of 30 professional cross-country skiing athletes were examined in three groups as athletes, female athletes, and male athletes. DNA of the participants were isolated from blood and genetic polymorphisms were determined by RT-PCR. Athletes were divided into two subgroups as faster and slower referring to their “1-kilometer cross-country skiing time averages (CCSTA)”. Polymorphism distributions in these subgroups were analyzed statistically with Fisher’s exact test and descriptive tests. In addition, the 1 km-CCSTA values of the genotypes were determined by descriptive statistical methods and the time advantages were calculated. It was determined that the combination of IL-6 rs1800795 GC and PPARA rs4253778 GG genotypes was observed to be more prominent among the faster categories of cross-country skiing competitors, particularly in the athletes and male athletes categories, and it had a time advantage at 1 km-CCSTA. The GC genotype (p= 0.0098) and C-allele (p=0.0398) of IL-6 rs1800795 polymorphism were detected at a higher rate in the fast subgroup in male athletes. These genotypes may support endurance performance.

PMID:38015538 | DOI:10.14715/cmb/2023.69.11.12

Cell Mol Biol (Noisy-le-grand). 2023 Nov 15;69(11):92-102. doi: 10.14715/cmb/2023.69.11.15.

ABSTRACT

We aimed to analyze the effect of acute exercise on oral microbiota in regularly trained swimmers. As environmental factors may affect the oral microbiota; we also aimed to analyze the short-duration effect of swimming training on the oral bacteria relative difference in swimmers. Saliva samples of 20 swimmers both before and after the training were used for the oral microbiota metagenesis. The next-generation sequencing method targeting 16S rDNA gene fragments was used for genotyping. The Wilcoxon signed-rank test was used for the statistical evaluation of the taxons. The alfa diversity comparisons were assessed with the One-Way ANOVA, and the Kruskal-Wallis test was used to determine bacterial diversity. Decayed- Missed- Filled total (DMF-T) scores were the indicators of oral hygiene. A comparison of the before and after exercise microbiota of the swimmers gave rise to a statistically significant difference for Firmicutes (p=0.014) and Bacteroidetes (p=0.007) phylum; Clostridia (p=0.006) and Bacilli (p=0.048) classes; Clostridiales (p=0.004), Entomoplasmatales (p=0.009) and Bacillales (p=0.006) for ordo; Lachnospiraceae (p=0.001) family and Stenotrophomonas (p=0.013) genus. Although there were some differences within the other taxa of the bacteria, all were statistically insignificant. Streptococcus, Pseudomonas and Rothia mucilaginosa showed a correlation with the DMF-T values in swimmers. This study was the first in Turkish swimmers to investigate the relative abundance of oral microbiota. We showed that exercise within the pool water changed the oral bacteria’s relative abundance. To confirm our results and clarify the effect of pool water on oral bacteria relative abundance, more studies on dietary intake should be carried out.

PMID:38015535 | DOI:10.14715/cmb/2023.69.11.15

JAMA Netw Open. 2023 Nov 1;6(11):e2344691. doi: 10.1001/jamanetworkopen.2023.44691.

ABSTRACT

IMPORTANCE: There is limited evidence of the association of conditional cash transfers, an important strategy to reduce poverty, with prevention of adverse birth-related outcomes.

OBJECTIVE: To investigate the association between receiving benefits from the Bolsa Família Program (BFP) and birth weight indicators.

DESIGN, SETTING, AND PARTICIPANTS: This cohort study used a linked data resource, the Centro de Integracao de Dados e Conhecimentos Para Saude (CIDACS) birth cohort. All live-born singleton infants born to mothers registered in the cohort between January 2012 and December 2015 were included. Each analysis was conducted for the overall population and separately by level of education, self-reported maternal race, and number of prenatal appointments. Data were analyzed from January 3 to April 24, 2023.

EXPOSURE: Live births of mothers who had received BFP until delivery (for a minimum of 9 months) were classified as exposed and compared with live births from mothers who did not receive the benefit prior to delivery.

MAIN OUTCOMES AND MEASURES: Low birth weight (LBW), birth weight in grams, and small for gestational age (SGA) were evaluated. Analytical methods used included propensity score estimation, kernel matching, and weighted logistic and linear regressions. Race categories included Parda, which translates from Portuguese as “brown” and is used to denote individuals whose racial background is predominantly Black and those with multiracial or multiethnic ancestry, including European, African, and Indigenous origins.

RESULTS: A total of 4 277 523 live births (2 085 737 females [48.8%]; 15 207 among Asian [0.4%], 334 225 among Black [7.8%], 29 115 among Indigenous [0.7%], 2 588 363 among Parda [60.5%], and 1 310 613 among White [30.6%] mothers) were assessed. BFP was associated with an increase of 17.76 g (95% CI, 16.52-19.01 g) in birth weight. Beneficiaries had an 11% lower chance of LBW (odds ratio [OR], 0.89; 95% CI, 0.88-0.90). BFP was associated with a greater decrease in odds of LBW among subgroups of mothers who attended fewer than 7 appointments (OR, 0.85; 95% CI, 0.84-0.87), were Indigenous (OR, 0.73; 95% CI, 0.61-0.88), and had 3 or less years of education (OR, 0.76; 95% CI, 0.72-0.81). There was no association between BFP and SGA, except among less educated mothers, who had a reduced risk of SGA (OR, 0.83; 95% CI, 0.79-0.88).

CONCLUSIONS AND RELEVANCE: This study found that BFP was associated with increased birth weight and reduced odds of LBW, with a greater decrease in odds of LBW among higher-risk groups. These findings suggest the importance of maintaining financial support for mothers at increased risk of birth weight-related outcomes.

PMID:38015506 | DOI:10.1001/jamanetworkopen.2023.44691

Ann Am Thorac Soc. 2023 Nov 28. doi: 10.1513/AnnalsATS.202309-753OC. Online ahead of print.

ABSTRACT

RATIONALE: Around 20-35% of obstructive sleep apnoea (OSA) patients show supine-isolated OSA, where supine sleep avoidance could be an effective therapy. However, traditional supine discomfort-based methods show poor tolerance and compliance to treatment, so cannot be recommended. Supine-alarm devices show promise, but evidence to support favourable adherence to treatment and effectiveness at reducing excessive daytime sleepiness compared to continuous positive airway pressure (CPAP) remains limited.

OBJECTIVES: To establish if alarm-based supine-avoidance treatment in supine-isolated OSA patients is non-inferior to CPAP in reducing daytime sleepiness.

METHODS: Following baseline questionnaires and in-home supine-time and polysomnography assessments, patients with supine-isolated OSA and Epworth sleepiness scale (ESS) score ≥8 were randomised to ≥6 weeks of supine-avoidance or CPAP treatment, followed by cross-over to the remaining treatment with repeat assessments. Non-inferiority was assessed from change in ESS with supine-avoidance compared to CPAP using a pre-specified non-inferiority margin of 1.5. Average nightly treatment use over all nights, and treatment efficacy and effectiveness at reducing respiratory disturbances were also compared between treatments.

RESULTS: The reduction in sleepiness score with supine-avoidance (mean [95%CI] -1.9 [-2.8 to -1.0]) was non-inferior to CPAP (-2.4 [-3.3 to -1.4], supine-avoidance-CPAP difference -0.4 [-1.3 to 0.6]) and the lower confidence limit did not cross the non-inferiority margin of 1.5, p=0.021. Average treatment usage was higher with supine-avoidance compared to CPAP (mean ± SD 5.7 ± 2.4 versus 3.9 ± 2.7 h/night, p<0.001).

CONCLUSIONS: In patients with supine-isolated OSA, vibro-tactile supine alarm device therapy is non-inferior to CPAP for reducing sleepiness and shows superior treatment adherence.

PMID:38015501 | DOI:10.1513/AnnalsATS.202309-753OC

Diagnosis (Berl). 2023 Nov 29. doi: 10.1515/dx-2023-0158. Online ahead of print.

ABSTRACT

OBJECTIVES: We have planned this analysis to provide current statistics on mortality directly caused by Influenza viruses in recent years in the US.

METHODS: We performed an electronic search in the online database CDC WONDER to obtain current statistics on direct mortality caused by Influenza viruses in the US. Mortality data are derived from information on all death certificates issued in the 50 states and the District of Columbia, excluding deaths of nonresidents. Our basic query criteria included Influenza-specific ICD-10 codes.

RESULTS: Influenza caused an average of 7,670 deaths per year from 2018 to 2020 based on Influenza-specific ICD-10 codes, with a corresponding mean death rate of 2.3 × 100,000. The death rate increased in parallel with the age of the US resident population, from 0.2 × 100,000 in the 5-24 age group to 37.4 × 100,000 in US residents aged 85 years or older. No substantial differences were observed in males vs. females.

CONCLUSIONS: The results of this analysis show that Influenza remains a significant clinical burden in the general population, with a cumulative mortality rate of approximately 2.3 × 100,000, but increasing more than tenfold (to over 37 × 100,000) in older persons.

PMID:38015489 | DOI:10.1515/dx-2023-0158

Ital J Dermatol Venerol. 2023 Dec;158(6):483-492. doi: 10.23736/S2784-8671.23.07653-3.

ABSTRACT

A recent research project using data from a total of 40 cancer registries has provided new epidemiologic insights into the results of efforts for melanoma control in Italy between the 1990s and the last decade. In this article, the authors present a summary and a commentary of their findings. Incidence increased significantly throughout the study period in both sexes. However, the rates showed a stabilization or a decrease in men and women aged below 35 years. The risk of disease increased for successive cohorts born until 1973 (women) and 1975 (men) while subsequently tending to decline. The trend towards decreasing tumor thickness and increasing survival has continued, but a novel favorable prognostic factor has emerged since 2013 for patients – particularly for males – with thick melanoma, most likely represented by molecular targeted therapies and immune checkpoint inhibitors. Due to this, the survival gap between males and females has been filled out. In the meanwhile, and despite the incidence increase, dermatologists have not lowered their threshold to perform skin biopsy. Skin biopsy rate has increased because of the increasingly greater volume of dermatologic office visits, but the proportion of skin biopsies out of dermatologic office visits has remained constant. In summary, an important breakthrough in melanoma control in Italy has taken place. Effective interventions have been implemented across the full scope of care, which involve many large local populations – virtually the whole national population. The strategies adopted during the last three decades represent a valuable basis for further steps ahead in melanoma control in Italy.

PMID:38015485 | DOI:10.23736/S2784-8671.23.07653-3

Eur J Neurol. 2023 Nov 28. doi: 10.1111/ene.16163. Online ahead of print.

ABSTRACT

BACKGROUND AND PURPOSE: We evaluated whether there was a difference in the occurrence of relapses pre- and post-COVID-19 vaccination in a nationwide cohort of Danish patients with relapsing multiple sclerosis.

METHODS: We conducted a population-based, nationwide cohort study with a cutoff date of 1 October 2022. We used McNemar tests to assess changes in the proportion of patients with recorded relapses within 90 days and 180 days before and after first vaccine dose, and a negative binomial regression model to compare the 90 and 180 days postvaccination annualized relapse rate (ARR) to the 360 days prevaccination ARR. Multivariate Cox regression was used to estimate relapse risk factors.

RESULTS: We identified 8169 vaccinated (87.3% Comirnaty) patients without a recorded history of a positive COVID-19 test. We did not find statistically significant changes in the proportion of patients with relapses in the 90 days (1.3% vs. 1.4% of patients, p = 0.627) and 180 days (2.7% vs. 2.6% of patients, p = 0.918) pre- and postvaccination. Also, a comparison of the ARR 360 days before (0.064, 95% confidence interval [CI] = 0.058-0.070) with the ARR 90 (0.057, 95% CI = 0.047-0.069, p = 0.285) and 180 (0.055, 95% CI = 0.048-0.063, p = 0.060) days after vaccination did not show statistically significant differences. Lower age, higher Expanded Disability Status Scale score, and relapse within 360 days before vaccination were associated with a higher risk of relapse.

CONCLUSIONS: We did not find evidence of increased relapse activity following the administration of the first dose of the COVID-19 vaccine.

PMID:38015454 | DOI:10.1111/ene.16163

Minerva Pediatr (Torino). 2023 Nov 28. doi: 10.23736/S2724-5276.23.07446-3. Online ahead of print.

ABSTRACT

BACKGROUND: The effect of different neonatal anthropometric charts on the incidence and neurodevelopmental outcomes at two years (Y) corrected age of small-for-gestational-age (SGA) preterm infants has still not been fully explored.

METHODS: All preterm infants with a gestational age (GA) between 24.0 and 31.6 weeks (W), born from Jan-2004 to Dec-2017 in the Marche region (Italy) were studied. Intergrowth-21^st, Beeby, Fenton, and Bertino anthropometric charts were used to classify infants with a birth weight less than 10^th centile as SGA. Disabilities and neurodevelopmental scores assessed by Bayley-III Test were recorded at the 2Y follow-up visit.

RESULTS: One thousand one hundred forty-seven preterm infants were evaluated. The incidence of SGA was significantly different among the study charts (from 12.9 to 17.5%). Nine hundred and twenty-seven study infants were assessed for neurodevelopmental outcomes at 2Y corrected age. The incidence of SGA with moderate cognitive impairment (COG Score: 70-84) and mild neurodevelopmental disability (NDD) were significantly different between the Intergrowth-21^st and Bertino charts (31.7% vs. 19.6%, P=0.042; 30.8 vs. 19.2%, P=0.036; respectively). A statistically significant difference in COG Score was found between SGA preterm infants overlapping in all study charts and those classified as SGA only by the Intergrowth-21^st chart (89.1±15.7 vs. 99.2±19.8; P=0.038).

CONCLUSIONS: In a large cohort of preterm infants with a GA between 24.0 and 31.6W, the incidence and neurodevelopmental outcomes at 2Y corrected age of SGAs were significantly different depending on the anthropometric charts. These differences, albeit small, should be considered both in clinical practice and trials on SGA preterm infants.

PMID:38015435 | DOI:10.23736/S2724-5276.23.07446-3

Nicotine Tob Res. 2023 Nov 28:ntad239. doi: 10.1093/ntr/ntad239. Online ahead of print.

ABSTRACT

INTRODUCTION: Non-cigarette tobacco (NCT) represents a form of tobacco use with a misperceived significance in chronic disease events. Whether NCT use is sufficient to promote stroke events, especially among Africans, is yet to be understood. This study assessed the relationship between NCT use and stroke among indigenous Africans.

METHODS: A total of 7,617 respondents (NCT users: 41 vs. non-NCT: 7576) from the Stroke Investigation Research and Educational Network study were included in the current analysis. NCT use was defined as self-reported use of smoked (cigars or piper) or smokeless (snuff or chewed) tobacco in the past year preceding stroke events. Stroke was defined based on clinical presentation and confirmed with a cranial CT/MRI. Multivariable-adjusted logistic regression was applied to estimate the odds ratio (OR) and 95% confidence interval (CI) for the relationship between NCT and stroke at p<0.05.

RESULTS: Out of the 41 (0.54%) who reported NCT use, 27 (65.9%) reported using smokeless NCT. NCT users were older than non-smokers (62.8±15.7 vs 57.7±14.8 years). Overall, NCT use was associated with first-ever stroke (OR: 2.08; 95%CI: 1.02, 4.23) in the entire sample. Notably, smokeless NCT use was independently associated with higher odds of stroke (OR: 2.74; 95%CI: 1.15, 6.54), but smoked NCT use (OR: 0.16; 95%CI: 0.02, 1.63) presented a statistically insignificant association after adjusting for hypertension and other covariates.

CONCLUSIONS: NCT use was associated with higher odds of stroke, and public health interventions targeting NCT use might be promising in reducing the burden of stroke among indigenous Africans.

IMPLICATIONS: A detailed understanding of the relationship between NCT use and stroke will likely inform well-articulated policy guidance to promote evidence-based recommendations for public health prevention and management of stroke on the African continent.

PMID:38015428 | DOI:10.1093/ntr/ntad239

J Cataract Refract Surg. 2023 Nov 27. doi: 10.1097/j.jcrs.0000000000001370. Online ahead of print.

ABSTRACT

PURPOSE: To compare actual and formula predicted postoperative refractive astigmatism using measured posterior corneal power measurements and four different empiric posterior corneal astigmatism correction models.

SETTING: Tertiary Care Center.

DESIGN: Single-center retrospective consecutive case series.

METHODS: Using a dataset of 211 eyes before and after tIOL implantation (Hoya Vivinex), IOLMaster (IOLM) or Casia2 (CASIA) keratometric and front / back surface corneal power measurements were converted to power vector components C0 (0/90°) and C45 (45/135°). Differences between postoperative and Castrop formula predicted refraction at the corneal plane using the labelled parameters of the tIOL and the keratometric or front / back surface corneal powers were recorded as the effect of corneal back surface astigmatism (BSA).

RESULTS: Generally, the centroid of the difference shifted towards negative C0 values indicating that BSA adds some against the rule corneal astigmatism (ATR). From IOLM / CASIA keratometry, the average difference in C0 was 0.39 / 0.32 dpt. After correction with the Abulafia-Koch, Goggin, La Hood, and Castrop nomograms it was -0.18 / -0.24 dpt, 0.27 / 0.18 dpt, 0.13 / 0.08 dpt, and 0.17 / 0.10 dpt. Using corneal front / back surface data from IOLM / CASIA, the difference was 0.18 / 0.12 dpt.

CONCLUSIONS: The Abulafia-Koch method over-corrected the ATR, while the Goggin, La Hood, and Castrop models slightly under-corrected ATR, and using measurements from the Casia2 tomographer seemed to produce slightly less prediction error than IOLMaster 700.

PMID:38015426 | DOI:10.1097/j.jcrs.0000000000001370