Category: Statistics

Sci Rep. 2026 May 10. doi: 10.1038/s41598-026-52703-4. Online ahead of print.

ABSTRACT

This study investigates variation in the phytochemical composition of Cedrus libani A. Rich. leaves across an altitudinal gradient, focusing on volatile and flavonoid profiles. Leaf samples were collected from three elevations (1300, 1500, and 1700 m) in the Yukarı Gökdere Forest region (Isparta, Turkey). Volatile compounds were analyzed using GC-MS, and flavonoid compounds were determined by RP-HPLC. A total of 45 volatile compounds were identified, predominantly consisting of monoterpenes and sesquiterpenes. Major components included limonene, β-myrcene, α-pinene, caryophyllene, and germacrene-D, with significant variation observed among elevation groups. One-way ANOVA indicated that several volatile compounds differed significantly across elevations (p < 0.05). Flavonoid analysis showed that rutin and kaempferol decreased with increasing elevation, whereas quercetin and catechin exhibited higher values at higher elevations. All analyzed flavonoid compounds showed statistically significant differences among elevation groups (p < 0.05). Principal component analysis (PCA) revealed separation patterns among samples based on their chemical composition. Overall, the results indicate that the phytochemical composition of C. libani leaves varies along the studied elevational gradient. These findings provide a preliminary and descriptive assessment of altitude-related chemical variation and highlight the need for further studies incorporating broader sampling designs and environmental measurements.

PMID:42108332 | DOI:10.1038/s41598-026-52703-4

Diabetologia. 2026 May 11. doi: 10.1007/s00125-026-06732-3. Online ahead of print.

ABSTRACT

AIMS/HYPOTHESIS: Chronic kidney disease (CKD) complicates insulin dosing and increases glycaemic instability in diabetes. We aimed to compare feasibility, safety and efficacy of automated insulin delivery (AID) with usual care in people with diabetes and advanced CKD.

METHODS: We conducted a prospective, open-label, randomised crossover trial at five tertiary hospitals in Australia and one tertiary centre in Denmark. Adults aged ≥18 years with type 1 diabetes or insulin-treated type 2 diabetes and advanced CKD (stage 3b or higher, including dialysis) were eligible. Participants were randomly assigned in a 1:1 sequence to receive either AID followed by usual care with real-time continuous glucose monitoring (CGM), or the reverse sequence, each for 8 weeks. Allocation was generated centrally using computerised randomisation. Due to the nature of the intervention, participants and clinicians were aware of treatment assignment. The primary outcome was percentage time in range (3.9-10.0 mmol/l) during the final 3 weeks of each treatment period.

RESULTS: Forty participants (24 type 1 diabetes, 16 type 2 diabetes; median [IQR] age 60 [55, 69] years; HbA_1c 64 [54, 73] mmol/mol [8.0% (7.1%, 8.8%)]; eGFR 30 [18, 37] ml/min per 1.73 m²) were enrolled: 33 not on dialysis, four on peritoneal dialysis and three on haemodialysis. AID significantly improved all hyperglycaemic CGM metrics compared with usual care. Time in range (3.9-10.0 mmol/l) improved from 60% (51%, 66%) at the end of usual care to 73% (65%, 78%) at the end of AID (p<0.001). Hypoglycaemia rates were unchanged. Participants were predominantly pre-frail at baseline and remained stable on-trial. No serious adverse events were attributed to the study devices. Nonetheless, 25% of participants experienced hospital admissions during the trial period for medical issues unrelated to device use.

CONCLUSIONS/INTERPRETATION: AID is feasible and safe and compared with usual care provides superior glucose management in predominantly pre-frail people with diabetes complicated by advanced CKD.

TRIAL REGISTRATION: Australian New Zealand Clinical Trials Registry ACTRN12622000889752; ClinicalTrials.gov NCT06330194 FUNDING: This trial was funded by the Australian Centre for Advancing Diabetes Innovations (ACADI), St Vincent’s Hospital Melbourne and Diabetes Australia.

PMID:42108331 | DOI:10.1007/s00125-026-06732-3

Eur J Orthop Surg Traumatol. 2026 May 11;36(1):189. doi: 10.1007/s00590-026-04774-2.

ABSTRACT

BACKGROUND: Testosterone replacement therapy (TRT) may cause side effects after orthopedic procedures. With total hip arthroplasty (THA) rates increasing, this study evaluates the relationship between TRT and postoperative complications in THA patients.

METHODS: A retrospective review in a large academic hospital was conducted of hypogonadal patients treated with TRT, who underwent primary, elective THA between 2012 and 2024. These were 1:2 propensity-matched based on age, body-mass index, and comorbidities to a “control” group that was not treated with TRT. Patient and TRT characteristics including serum testosterone levels, form of administration, 90-day emergency department visits (ED) and readmissions, reoperations and revisions were explored.

RESULTS: Among 152 patients aged 61.3 years who underwent THA with a 2.7-year follow-up, TRT was mainly administered intramuscularly (51.3%) or via transdermal gel (46.1%), followed by pellets (2.0%), and oral tablets (1.6%). Overall rates of 90-day ED visits and readmissions did not differ significantly between TRT and control patients (7.9% vs. 5.3%, P = 0.270 and 7.9% vs. 5.6%, P = 0.225, respectively). TRT patients had a significantly lower rate of 90-day ED visits due to surgery-related causes (0.7% vs. 2.3%, P = 0.048) but a significantly higher rate due to non-surgery-related causes (7.2% vs. 3.0%, P = 0.034). The incidence of PJI did not differ significantly between the groups (2.0% vs. 1.0%, P = 0.319). Reoperations and revisions were not different between the groups (P = 0.650 and P = 0.057, respectively). TRT administration form was not associated with 90-day ED visits (P = 0.380), readmissions (P = 0.563), reoperations (P = 0.441) or revisions (P = 0.669). Testosterone levels demonstrated a weak, negative, yet significant correlation with 90-day ED visits (r = -0.35, P = 0.040), but not with reoperations or revisions (P = 0.348 and P = 0.431, respectively).

CONCLUSIONS: TRT in THA patients was associated with a reduced rate of surgery-related 90-day ED visits but an increased rate of non-surgery-related 90-day ED visits. Incidence of PJI and overall 90-day ED visits and readmission rates did not significantly differ. Administration form had no significant impact, while higher testosterone levels were linked to fewer 90-day ED visits. Although limited by its retrospective design and patient exclusions, further investigation is warranted to guide perioperative management in these patients, particularly given the known immunomodulatory effects of exogenous TRT.

PMID:42108328 | DOI:10.1007/s00590-026-04774-2

Emerg Radiol. 2026 May 11. doi: 10.1007/s10140-026-02475-1. Online ahead of print.

ABSTRACT

PURPOSE: To evaluate the clinical associations of admission non-contrast CT morphology in acute pancreatitis within a real-world emergency workflow.

MATERIALS AND METHODS: This retrospective observational cohort study included 264 consecutive adult patients admitted with acute pancreatitis to two surgical centers between 2019 and 2024. Patients were categorized according to the first imaging modality obtained at admission into an ultrasound-first (US-first) or computed tomography-first (CT-first) pathway. Baseline characteristics and in-hospital outcomes were compared between pathways. In the CT-first subgroup, all examinations were performed without intravenous contrast, and morphologic severity was assessed using the Balthazar classification. Associations between CT morphology and clinical outcomes were evaluated using univariable analyses.

RESULTS: Of the 264 patients, 143 (54.2%) were managed within a US-first pathway and 121 (45.8%) underwent CT as the initial imaging modality. Baseline demographic and etiologic characteristics were comparable between pathways. Patients in the CT-first pathway demonstrated numerically higher rates of adverse clinical outcomes at admission, including a longer length of hospital stay (median 8 vs. 6 days; p = 0.01) and numerically higher rates of severe acute pancreatitis and in-hospital mortality. Within the CT-first cohort, non-contrast CT morphology demonstrated heterogeneous inflammatory severity. Higher Balthazar grades were associated with stepwise numerical increases in rates of severe disease, complications, and length of hospital stay. When dichotomized, advanced morphologic severity (Balthazar grades D-E) showed higher odds of adverse outcomes compared with grades A-C, although these associations did not reach statistical significance.

CONCLUSION: In routine emergency practice, selection of ultrasound-first or CT-first imaging pathways appears largely driven by triage and organizational factors rather than predefined imaging strategies. In patients undergoing non-contrast CT at admission, higher Balthazar grades demonstrated consistent numerical gradients toward more severe clinical courses; however, these associations did not reach statistical significance. Early non-contrast CT morphology should therefore be interpreted as contextual inflammatory assessment rather than a standalone prognostic tool.

PMID:42108327 | DOI:10.1007/s10140-026-02475-1

Brain Inform. 2026 May 11. doi: 10.1186/s40708-026-00302-4. Online ahead of print.

ABSTRACT

Accurate detection of Mild Cognitive Impairment (MCI) is critical for timely intervention and for slowing progression to Alzheimer’s disease. Electroencephalography (EEG) offers a non-invasive and cost-effective measure of brain activity; however, its complex, non-linear dynamics limit conventional analysis. We propose a CNN-Res-SE-BiLSTM-BiGRU framework for the automated detection of MCI directly from raw EEG. Convolutional and residual blocks capture local temporal structure, bidirectional recurrent layers model long-range dependencies, and Squeeze-and-Excitation (SE) modules provide channel-wise attention. Predicted probabilities are calibrated using temperature scaling, and operating thresholds are selected on the validation set using Youden’s J statistic. The model is evaluated using five-fold cross-validation under both subject-dependent and strict subject-independent protocols on a primary resting-state dataset, with additional subject-independent validation on an odor EEG dataset. Under subject-independent evaluation on the odor dataset, the proposed model achieved an accuracy of 0.956 ± 0.051, with ROC-AUC of 0.971 ± 0.051 and PR-AUC of 0.934 ± 0.132. UMAP-based visualization and explainable AI analyses (SHAP and LIME) provide interpretable insight into the learned spatiotemporal patterns and sample-specific decisions. These results demonstrate robust, interpretable EEG-based MCI detection with potential clinical utility.

PMID:42108320 | DOI:10.1186/s40708-026-00302-4

Eur J Orthop Surg Traumatol. 2026 May 11;36(1):190. doi: 10.1007/s00590-026-04773-3.

ABSTRACT

PURPOSE: Ankle fractures are among the most common orthopedic injuries requiring operative management. Postoperative care varies across healthcare systems due to cultural, regulatory, and practice-based differences. This study evaluated whether such variation influences ankle fracture healing.

METHODS: We conducted a retrospective cohort study at two Level 1 trauma centers, one in the United States and one in Chile, from 2015 to 2023. Patients included had sustained Weber B or C ankle fractures due to falls and were treated with ORIF, with available 3- and 6-month radiographs. Demographic, clinical, and operative variables were recorded. Genetic matching was used to balance key covariates between cohorts.

RESULTS: Of 250 patients, 110 remained after genetic matching (55 pairs) with well-balanced baseline characteristics (all standardized mean differences < 0.1). No significant differences in fracture union time were observed between cohorts in either unmatched or matched analyses. In the matched cohort, mean union time was 108.6 ± 60.6 days in the U.S. group and 124.8 ± 88.2 days in the Chilean group (p = 0.235). Sensitivity analyses demonstrated consistent findings. Complication rates were similar, with no differences in infection, delayed union, or nonunion; however, a higher proportion of Chilean patients had no complications (72.7 vs. 50.9%, p = 0.038).

CONCLUSION: In this matched binational cohort, patients with operatively treated ankle fractures demonstrated comparable healing outcomes despite differences in postoperative care strategies across healthcare systems. These findings highlight the reliability of fracture healing in this injury pattern with low rate of complications despite cultural and system-level variation, supporting opioid-sparing strategies in appropriately selected patients.

PMID:42108318 | DOI:10.1007/s00590-026-04773-3

J Chem Ecol. 2026 May 11;52(3):41. doi: 10.1007/s10886-026-01716-4.

ABSTRACT

Availability and quality of vegetation are critical factors influencing herbivore nutrition and population dynamics. Fourier-transform infrared spectroscopy (FTIR) offers a promising approach to analyze herbivore diets using spectral properties of phytochemicals to identify plant items. We evaluated the potential of FTIR to identify plant taxa and parts consumed by an herbivore species. Crop contents from 236 rock ptarmigan (Lagopus muta MONTIN) individuals from Iceland, collected over nine years, were separated into pure fractions of plant taxa and parts (e.g., berries, leaves) and analyzed using FTIR in the mid-IR region (4000 -400 cm⁻¹). We classified plant taxa and parts with PCA and Random Forests (RF) based on spectral signals. FTIR revealed distinct chemical fingerprints for plant taxa and parts, consistent with previously established variation in lipids, proteins, carbohydrates, and chemical defenses. RF yielded high classification accuracy for plant parts (96.7%) and moderate accuracy for taxa (85.5%), confirming the method’s reliability. FTIR overcomes limitations of traditional genetic analyses by identifying plant parts with varying nutritional quality within species. FTIR provided insights into biochemical properties of plant items but could not distinguish chemically similar items. Future research should expand spectral reference libraries combining FTIR with quantification of phytochemicals and DNA metabarcoding.

PMID:42108314 | DOI:10.1007/s10886-026-01716-4

Int J Comput Assist Radiol Surg. 2026 May 11. doi: 10.1007/s11548-026-03645-9. Online ahead of print.

ABSTRACT

PURPOSE: Myocardium segmentation in echocardiography videos is a challenging task due to low contrast, noise, and anatomical variability. Traditional deep learning models either process frames independently, ignoring temporal information, or rely on memory-based feature propagation, which accumulates error over time.

METHODS: We propose PointSeg, a transformer-based segmentation framework that integrates point tracking as a temporal cue to ensure stable and consistent segmentation of myocardium across frames. Our method leverages a point-tracking module trained on a synthetic echocardiography dataset to track key anatomical landmarks across video sequences. These tracked trajectories provide an explicit motion-aware signal that guides segmentation, reducing drift and eliminating the need for memory-based feature accumulation. Additionally, we incorporate a temporal smoothing loss to further enhance temporal consistency across frames.

RESULTS: We evaluate our approach on both public and private echocardiography datasets. Experimental results demonstrate that PointSeg has statistically similar accuracy in terms of Dice to state-of-the-art segmentation models in high-quality echo data, while it achieves better segmentation accuracy in lower-quality echo with improved temporal stability. Furthermore, PointSeg has the key advantage of pixel-level myocardium motion information as opposed to other segmentation methods. Such information is essential in the computation of other downstream tasks such as myocardial strain measurement and regional wall motion abnormality detection.

CONCLUSION: PointSeg demonstrates that point tracking can serve as an effective temporal cue for consistent video segmentation, offering a reliable and generalizable approach for myocardium segmentation in echocardiography videos. The code is available at https://github.com/DeepRCL/PointSeg .

PMID:42108311 | DOI:10.1007/s11548-026-03645-9

Nat Med. 2026 May 10. doi: 10.1038/s41591-026-04408-4. Online ahead of print.

ABSTRACT

MicroRNA-132 (miR-132) is a central regulator of adverse cardiac remodeling. Here we evaluated CDR132L, a synthetic antisense oligonucleotide miR-132 inhibitor, in a multinational, randomized, double-blind, placebo-controlled phase 2 trial (HF-REVERT) in patients with recent myocardial infarction (MI) and left ventricular (LV) systolic dysfunction. Within 3-14 days after MI, 294 patients were randomized to receive CDR132L 5 mg kg^-1, CDR132L 10 mg kg^-1 or placebo as three intravenous doses at 4-week intervals plus guideline-directed therapy. In total, 280 patients (245 men and 35 women) who received at least one dose of the study drug were included in the modified intention-to-treat population. CDR132L was well tolerated, with no hepatic, renal, hematologic or cardiac toxicity signals. The primary endpoint-the percentage change in LV end-systolic volume index at 6 months-improved in all groups but did not differ significantly between the CDR132L groups (5 mg kg^-1 and 10 mg kg^-1) and the placebo group. Secondary endpoints, including LV ejection fraction, global longitudinal strain and N-terminal pro B-type natriuretic peptide, were also not significantly different between the CDR132L and placebo groups. Prespecified exploratory analyses suggested potential benefits of CDR132L treatment in patients with advanced adverse remodeling at baseline, supporting further evaluation of CDR132L, including in chronic heart failure conditions. ClinicalTrials.gov: NCT05350969 .

PMID:42108271 | DOI:10.1038/s41591-026-04408-4

Sci Rep. 2026 May 10. doi: 10.1038/s41598-026-51415-z. Online ahead of print.

ABSTRACT

Cholera is highly climate sensitive, however previous attempts to model its future under climate change have been limited to statistical analyses. Mechanistic models are an essential addition because they permit a deeper understanding of the complex feedback loops involved in infectious disease transmission, allowing for better modelling of potential scenarios such as interventions or changes in pathogen dynamics. We compare four mathematical models with differing assumptions of climate sensitivity and fit them to a cholera dataset from Kolkata, India using MCMC. We then use bias-corrected climate projections of temperature and rainfall from 10 independent global climate models to produce climate-based cholera projections for the period 2080-2099. Using both temperature and rainfall as inputs, the best performing model recreates seasonal patterns highly effectively. Future projections suggest an average increase in cholera cases ranging from 81% – 150% due to climate change by 2080-2099 with earlier peaks in the infection cycle likely due to heightened transmission rates earlier in the year. Sensitivity analysis reveals that uncertainties in parameters related to the contact rate and water dynamics have the greatest impact on model projections, suggesting that these factors are critical for refining future predictions. While our mechanistic model demonstrates the potential to project cholera dynamics under future climate scenarios, projections remain sensitive to key knowledge gaps including epidemiological parameters and effects of temperature on bacterial growth. Addressing these limitations through improved environmental observations and more detailed process representation will be essential for refining future climate-cholera projections and informing long-term control strategies.

PMID:42108242 | DOI:10.1038/s41598-026-51415-z