Category: Statistics

Sci Rep. 2023 Feb 23;13(1):3177. doi: 10.1038/s41598-023-27603-6.

ABSTRACT

It is difficult to directly obtain pathological diagnosis of perihilar cholangiocarcinoma (pCCA). Analysis of bile in the pCCA microenvironment, based on metabolomics and statistical methods, can help in clinical diagnosis. Clinical information, bile samples, blood liver function, blood CA199, CEA, and other indicators were collected from 33 patients with pCCA and 16 patients with gallstones. Bile samples were analyzed using untargeted metabolomics methods. A combination of multivariate and univariate analyses were used to screen for potential differential metabolites Through Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and differential metabolite remodeling, we explored changes in the pCCA pathway and potential therapeutic targets. There were significant differences in patient blood TBIL, ALT, AST, TBA, CA19-9, and CEA indices (p < 0.05, |log2(fc)| ≥ 1) between two groups. A significant correlation was found between these different indicators by Spearman’s analysis. The clinical parameters were correlated with mass-to-charge ratios of 305 (Positive Ion Mode, POS) and 246 (Negative Ion Mode, NEG) in the metabolic group (|r| ≥ 0.7, P ≤ 10^-7). The result of this study indicated that bile untargeted metabolomics combined with statistical analysis techniques may be used for diagnose and treatment of pCCA.

PMID:36823159 | DOI:10.1038/s41598-023-27603-6

Nihon Hoshasen Gijutsu Gakkai Zasshi. 2023 Feb 21. doi: 10.6009/jjrt.2023-1331. Online ahead of print.

ABSTRACT

PURPOSE: Dual-energy computed tomography (DECT) system can generate virtual non-contrast (VNC) images. Although several reconstruction algorithms are defined, there are not many researches using deep learning image reconstruction (DLIR) algorithm. In this study, we evaluated the accuracy of the VNC image reconstruction under various conditions using DLIR algorithm.

METHODS: At first, each iodine insert with variable concentrations (2.0, 5.0, 10.0, 15.0 mg/ml) or diameters (2.0, 5.0, 10.0, 28.5 mm), or mixed insert including blood-mimicking material with iodine (iodine concentrations: 2.0, 4.0 mg/ml) was put in the center of the multi-energy CT phantom (Gammex, USA). This phantom was placed in the isocenter of DECT, and it scanned and reconstructed the VNC images. In addition, the VNC images were reconstructed with various display field of view (DFOV) sizes (240, 350 mm) or reconstruction algorithms (filtered back projection, advanced statistical iterative reconstruction, deep learning image reconstruction) for each iodine diameter. Attenuation values of these images (CT_VNC) were measured and assessed by placing a circular region of interest (ROI) on each insert.

RESULTS: CT_VNC form iodine inserts increased with iodine concentration became lower, whereas CT_VNC form blood plus iodine inserts were stable regardless of low iodine concentration. As iodine diameter became smaller, CT_VNC increased remarkably. CT_VNC remained steady even though reconstruction parameters were varied.

CONCLUSION: In our study, the VNC image reconstruction using DLIR algorithm was affected by various conditions such as iodine concentration and size. In particular, its accuracy was reduced by the size of target.

PMID:36823148 | DOI:10.6009/jjrt.2023-1331

Transl Psychiatry. 2023 Feb 23;13(1):68. doi: 10.1038/s41398-023-02367-9.

ABSTRACT

Depression is one of the leading causes of disability worldwide and is a major contributor to the global burden of disease among older adults. The study aimed to investigate the interplay between socio-economic markers (education and financial resources) and polygenic predisposition influencing individual differences in depressive symptoms and their change over time in older adults, which is of central relevance for preventative strategies. The sample encompassing n = 6202 adults aged ≥50 years old with a follow-up period of 14 years was utilised from the English Longitudinal Study of Ageing. Polygenic scores for depressive symptoms were calculated using summary statistics for (1) single-trait depressive symptoms (PGS-DS_single), and (2) multi-trait including depressive symptoms, subjective well-being, neuroticism, loneliness, and self-rated health (PGS-DS_multi-trait). The depressive symptoms over the past week were measured using the eight-item Centre for Epidemiologic Studies Depression Scale. One standard deviation increase in each PGS was associated with a higher baseline score in depressive symptoms. Each additional year of completed schooling was associated with lower baseline depression symptoms (β = -0.06, 95%CI = -0.07 to -0.05, p < 0.001); intermediate and lower wealth were associated with a higher baseline score in depressive symptoms. Although there was a weak interaction effect between PGS-DSs and socio-economic status in association with the baseline depressive symptoms, there were no significant relationships of PGS-DSs, socio-economic factors, and rate of change in the depressive symptoms during the 14-year follow-up period. Common genetic variants for depressive symptoms are associated with a greater number of depressive symptoms onset but not with their rate of change in the following 14 years. Lower socio-economic status is an important factor influencing individual levels of depressive symptoms, independently from polygenic predisposition to depressive symptoms.

PMID:36823133 | DOI:10.1038/s41398-023-02367-9

J Nippon Med Sch. 2023 Feb 21. doi: 10.1272/jnms.JNMS.2023_90-213. Online ahead of print.

ABSTRACT

BackgroundAlthough coronary artery disease (CAD) is characterized by epicardial atherosclerosis and microvascular disease, the importance of evaluating microvascular dysfunction has not been sufficiently recognized in clinical practice. We estimated microvascular disease severity by assessing the hyperemic microvascular resistance (MVR) via absolute quantification of myocardial blood flow (MBF) with ¹³N-ammonia positron emission tomography-myocardial perfusion imaging (PET-MPI).MethodsWe retrospectively collected the data for 23 CAD patients who underwent both stress/rest PET-MPI and invasive coronary angiography (CAG) with fractional flow reserve (FFR) measurement. Among 30 vessels for which FFR measurement was performed, 13 showed significantly low FFR (FFR ≤ 0.75). In each patient, myocardial segments of a standard 17-segment model were assigned to the stenotic myocardial area perfused by the FFR-measured vessel and a reference normal-perfusion area based on PET-MPI and the coronary distribution on CAG. Hyperemic MVR was calculated using the formula “hyperemic MVR = hyperemic mean blood pressure × FFR/hyperemic MBF of the stenotic vessel.”ResultsA strong statistically significant negative correlation was observed between hyperemic MVR and hyperemic MBF in the reference normal-perfusion area (R = -0.758, P<0.001).ConclusionMicrovascular disease severity in chronic CAD may be estimated by hyperemic MBF of the normal-perfusion area with ¹³N-ammonia PET-MPI.

PMID:36823123 | DOI:10.1272/jnms.JNMS.2023_90-213

Occup Environ Med. 2023 Feb 23:oemed-2022-108609. doi: 10.1136/oemed-2022-108609. Online ahead of print.

ABSTRACT

OBJECTIVES: This study aimed to identify whether differences exist in postpartum depression (PPD) in US and Korean nurses and its related factors. Identifying occupational and personal factors that underlie potential differences will be helpful for women’s occupational health.

METHODS: Baseline and postpartum survey data from employed nurses in the Korea Nurses’ Health Study and Nurses’ Health Study 3 (1244 Korean; 2742 US nurses) were analysed. Postpartum data collection was done via online survey. PPD was analysed based on cultural validation from prior studies using the Edinburgh Postnatal Depression Scale (cut-off of 10 for Korea and 13 for USA); depressive symptoms prior to pregnancy and childbirth, general characteristics and sleep satisfaction were also measured. Descriptive statistics, χ² tests and t-tests and multivariate ordinal logistic regression analysis were performed.

RESULTS: 45.9% of Korean participants had clinical symptoms of PPD (≥10), whereas US participants presented with 3.4% (≥13). Prior depressive symptoms were also higher in Korean participants (22.5%) compared with their US counterparts (4.5%). Prior depressive symptoms and poor sleep satisfaction were significant risk factors of PPD in both cohort groups, and vaginal birth was an additional influencing factor in Korean participants.

CONCLUSIONS: Differences in PPD rates and related factors suggest the role of stress, cultural variation and differing work systems. Nurses and other women shift-workers noted to have depressive symptoms before and during pregnancy and exhibit PPD symptoms should especially be followed closely and offered supportive mental health services that include greater flexibility in returning to work.

PMID:36823104 | DOI:10.1136/oemed-2022-108609

Intern Med. 2023 Feb 22. doi: 10.2169/internalmedicine.0912-22. Online ahead of print.

ABSTRACT

Objective In recent decades, living conditions have changed drastically. However, there are few data regarding the interaction between living conditions and the risk of ischemic stroke (IS) in young adults. The present study explored the association between living conditions or marital status and the risk factors, etiology, and outcome of IS in young adults. Methods We prospectively enrolled patients with incident IS who were 20-49 years old from 37 clinical stroke centers. We collected the demographic data, living conditions, marital status, vascular risk factors, disease etiology, treatment, and outcomes at discharge. A comparison group was established using the official statistics of Japan. We categorized patients into the two groups based on living conditions: solitary group and cohabiting group. Clinical characteristics were then compared between living conditions. Results In total, 303 patients were enrolled (224 men; median age at the onset: 44 years old). Significant factors associated with the incidence of IS were as follows: solitary status, body mass index >30 kg/m², current smoking, heavy alcohol consumption, hypertension, diabetes mellitus, and dyslipidemia. Furthermore, in the solitary group, the proportions of men, unmarried individuals, and current smokers were significantly higher than in the cohabiting group. In addition, poor outcomes (modified Ranking Scale ≥ 4) of IS were more common in the solitary group than in the cohabiting group. Conclusion Our study showed that not only conventional vascular risk factors but also living conditions, especially living alone while unmarried, were independent risk factors for IS in young adults.

PMID:36823091 | DOI:10.2169/internalmedicine.0912-22

Circ J. 2023 Feb 21. doi: 10.1253/circj.CJ-22-0682. Online ahead of print.

ABSTRACT

BACKGROUND: Fluoroquinolone use can be associated with an increased risk of aortic aneurysm (AA) or aortic dissection (AD). The US Food and Drug Administration recently warned against fluoroquinolone use for high-risk patients, such as those with Marfan syndrome. However, the association between fluoroquinolone use and AA/AD risk was unknown in these high-risk patients and therefore it was studied in this work.Methods and Results: Data were collected from a national database between 2000 and 2017 for 550 patients with AA/AD and any congenital aortic disease (mean age 41.5 years; 415 with Marfan syndrome). A case cross-over study was conducted to compare the risk of aortic events (AA/AD) associated with fluoroquinolone and amoxicillin use between the hazard period (from -60 days to -1 day) and a randomly selected reference period (-180 to -121 days; -240 to -181 days; and -300 to -241 days). Compared to the reference period without fluoroquinolone use, fluoroquinolone use during the hazard period was not associated with a greater risk of AA/AD (1.09% vs. 1.09%; odds ratio [OR] 1.000; 95% confidence interval [CI] 0.32-3.10), AA (OR 0.67; 95% CI 0.11-3.99), or AD (OR 1.33; 95% CI 0.30-5.96) in patients with congenital aortic disease or Marfan syndrome. This lack of association was maintained in subgroup analysis, including Marfan syndrome or not, age (≤50 vs. >50 years) and sex.

CONCLUSIONS: Fluoroquinolone use was not associated with an increased risk of AA/AD in patients with congenital aortic disease, including Marfan syndrome. More evidence is required for a fluoroquinolone pharmacovigilance plan in these patients.

PMID:36823078 | DOI:10.1253/circj.CJ-22-0682

Psychol Rep. 2023 Feb 23:332941231159611. doi: 10.1177/00332941231159611. Online ahead of print.

ABSTRACT

The COVID-19 pandemic and its link to the emergence of everyday sadism is a matter of public concern worldwide. However, previous studies are nearly silent regarding the causal relationship between the two variables. We address this gap by theorizing that exposure to information about coronavirus can increase sadistic behavior by inducing state boredom. We conducted three complementary controlled experiments, which comprised multiple participants populations (N = 784, student and community samples) and measurement techniques of sadism, to test our theoretical perspective. Based on self-report measures, Study 1 found that Chinese university students who were exposed to a reminder of COVID-19 exhibited a higher level of everyday sadism than participants in the control condition. Study 2 replicated this finding in a more generalized population. Additionally, results revealed that state boredom mediated this effect. Moving beyond subjective self-report data in Studies 1 and 2, Study 3 assessed a different behavioral operationalization of sadistic tendencies, namely, shredding worms. As expected, priming COVID-19 salience has an immediate, statistically significant influence on sadistic behavior in impactful real-world contexts. Overall, these findings suggest that the COVID-19 pandemic not only has grave effects on economy and society, but has implications for the malevolent side of human nature.

PMID:36823028 | DOI:10.1177/00332941231159611

Clin Genitourin Cancer. 2023 Jan 20:S1558-7673(23)00025-3. doi: 10.1016/j.clgc.2023.01.005. Online ahead of print.

ABSTRACT

Pembrolizumab, a PD-1 ICI is approved for the adjuvant treatment of postnephrectomy patients with clear cell RCC in some countries worldwide. However, recent negative data from randomized clinical trials (RCT) with another ICIs makes the benefit of this treatment uncertain. A systematic review and study-level meta-analysis was performed to evaluate the benefit of disease-free survival (DFS) with adjuvant ICI treatment for patients with localized and/or metastatic resected RCC. Using the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) statement, a systematic search was performed in PUBMED/MEDLINE, Scopus and EMBASE up to September 15, 2022. The statistical analysis was performed by ProMeta 3 software in intention-to-treat (ITT) population and in predetermined subgroups. Four RCT totalizing 3407 patients were included in this analysis. Systemic immunotherapy was pembrolizumab, atezolizumab, nivolumab, and ipilimumab plus nivolumab in 496, 390, 404, and 405 patients, respectively. In the ITT population there was a nonstatistically significant DFS benefit with adjuvant ICI (HR: 0.85, 95% CI: 0.69-1.04). Regarding the subgroups, there was a DFS benefit for PD-L1 positive (HR: 0.72; 95% CI: 0.55-0.94), intermediate-high risk patients (HR: 0.77; 95% CI: 0.63-0.94), and patients with sarcomatoid component (HR: 0.66; 95% CI: 0.43-0.99). This meta-analysis did not demonstrate a statistically significant DFS benefit in overall population, however considering the heterogeneity between the RCTs the use of adjuvant ICI should be individualized.

PMID:36823017 | DOI:10.1016/j.clgc.2023.01.005

J Dairy Sci. 2023 Feb 21:S0022-0302(23)00070-X. doi: 10.3168/jds.2022-22567. Online ahead of print.

ABSTRACT

Four categories of transfer of passive immunity (TPI) were recently proposed in response to the widespread high preweaning morbidity and mortality risks in calves with adequate TPI when a dichotomous classification was used. Hitherto, however, the risks of preweaning morbidity and mortality and future performance among these TPI categories have not been compared. Thus, the objective of this retrospective cohort study was to compare dairy calf morbidity, mortality, growth until weaning, and reproductive efficiency until first calving among the categories of poor (<5.1 g/dL total protein), fair (5.1-5.7 g/dL), good (5.8-6.1 g/dL), and excellent (>6.2 g/dL) TPI. For this, the records from 4,336 dairy calves (2,272 female, 2,064 male) born January 2014 to April 2017 on a commercial dairy farm in Michigan were analyzed. These calves had been randomly selected for weekly serum total protein determination on calves 2 to 7 d old. Data from both sexes were used to evaluate preweaning health and mortality, whereas only the female’s data were used to investigate average daily gain (ADG), reproductive performance, and first-lactation milk yield. For each calf, data regarding disease status, growth, and reproductive parameters were obtained from the farm’s software database. Associations of TPI categories with disease events (diarrhea or pneumonia), reproduction indices (age at first insemination, successful insemination, and calving, and number of inseminations), first-lactation milk yield and ADG at weaning were evaluated by survival analysis and mixed models. Compared with calves with excellent TPI, calves in the inferior TPI categories showed increased risk of diarrhea: poor [hazard ratio (HR) = 1.49; 95% CI: 1.22-1.82], fair (HR = 1.32; 95% CI: 1.16-1.51), good (HR = 1.14; 95% CI: 1.02-1.29). However, the risk of pneumonia differed only between the calves in the poor and excellent TPI groups (HR = 1.39; 95% CI: 1.05-1.84). The preweaning mortality risk was also higher in calves with poor TPI (HR = 4.29; 95% CI: 1.98-9.27) compared with excellent TPI. However, mortality risks were not statistically different between calves with fair or good TPI and those with excellent TPI. Similarly, calves with poor TPI had a 64, 55, and 24% lower risk of reaching first insemination, successful insemination, or first calving, respectively. However, there were no differences in ADG, number of inseminations, or first-lactation 305-d mature equivalent milk production across TPI groups. Our results confirm the positive effects of optimal TPI in calf preweaning health and postweaning reproductive efficiency. The 4 proposed categories of TPI can assist in decreasing the incidence of diseases that occur in the first weeks of life (i.e., diarrhea), but their effect on other diseases or future performance might be more limited. Although conducted in one herd, this study can be used to illustrate the effect of TPI on future calf performance.

PMID:36823003 | DOI:10.3168/jds.2022-22567