Category: Statistics

Genes Genomics. 2022 Jul 8. doi: 10.1007/s13258-022-01277-2. Online ahead of print.

ABSTRACT

BACKGROUND: Metabolic syndrome is as a well-known risk factor for cardiovascular disease, which is associated with both genetic and environmental factors. Recently, the microbiome composition has been shown to affect the development of metabolic syndrome. Thus, it is expected that the complex interplay among host genetics, the microbiome, and environmental factors could affect metabolic syndrome.

OBJECTIVE: To evaluate the relative contributions of genetic, microbiome, and environmental factors to metabolic syndrome using statistical approaches.

METHODS: Data from the prospective Korean Association REsource project cohort (N = 8476) were used in this study, including single-nucleotide polymorphisms, phenotypes and lifestyle factors, and the urine-derived microbial composition. The effect of each data source on metabolic phenotypes was evaluated using a heritability estimation approach and a prediction model separately. We further experimented with various types of metagenomic relationship matrices to estimate the phenotypic variance explained by the microbiome.

RESULTS: With the heritability estimation, five of the 11 metabolic phenotypes were significantly associated with metagenome-wide similarity. We found significant heritability for fasting glucose (4.8%), high-density lipoprotein cholesterol (4.9%), waist-hip ratio (7.7%), and waist circumference (5.6%). Microbiome compositions provided more accurate estimations than genetic factors for the same sample size. In the prediction model, the contribution of each source to the prediction accuracy varied for each phenotype.

CONCLUSION: The effects of host genetics, the metagenome, and environmental factors on metabolic syndrome were minimal. Our statistical analysis suffers from a small sample size, and the measurement error is expected to be substantial. Further analysis is necessary to quantify the effects with better accuracy.

PMID:35802345 | DOI:10.1007/s13258-022-01277-2

Eat Weight Disord. 2022 Jul 8. doi: 10.1007/s40519-022-01436-0. Online ahead of print.

ABSTRACT

PURPOSE: In this study, it was aimed to evaluate the relationship of hedonic hunger with food addiction and obesity in university students.

METHODS: The research sample consists of 275 university students between the ages of 19-28 years. Body weight (kg), height (cm), waist and hip circumference (cm) were measured by applying a face-to-face questionnaire including descriptive characteristics of individuals, the Power of Food Scale (PFS), the Palatable Eating Motive Scale (PEMS) and the Modified Yale Food Addiction Scale (mYFAS) 2.0. Statistical analysis of the data was done with SPSS (Statistical Package for Social Sciences) 18.

RESULTS: In individuals, according to the mean PFS score the presence of food power-induced hedonic hunger was detected. While according to the mean PEMS score no motivational hedonic hunger was detected. According to the BMI classification the average scores of PFS (p = 0.002), PEMS (p = 0.009), and mYFAS 2.0 (p < 0.001) showed a gradual increase from underweight to obese. A weak positive correlation was found between BMI and PFS score (r = 0.238; p < 0.05) and PEMS score (r = 0.196; p < 0.05), respectively. The moderate positive correlation (r = 0.439 p < 0.001) was found between mYFAS 2.0 and the PEMS scores.

CONCLUSION: As a result, the presence of food-based hedonic hunger was determined in young individuals. A relationship was determined between food addiction and hedonic hunger caused by the motivation to consume delicious foods. Accordingly, hedonic hunger is related to food addiction and obesity in university students.

LEVEL OF EVIDENCE: V, cross-sectional descriptive study.

PMID:35802338 | DOI:10.1007/s40519-022-01436-0

Environ Sci Pollut Res Int. 2022 Jul 8. doi: 10.1007/s11356-022-21844-0. Online ahead of print.

ABSTRACT

In this study, luminescent bio-adsorbent nitrogen-doped carbon dots (N-CDs) was produced and applied for the removal and detection of Hg (II) from aqueous media. N-CDs were synthesized from oil palm empty fruit bunch carboxymethylcellulose (CMC) and urea. According to several analytical techniques used, the obtained N-CDs display graphitic core with an average size of 4.2 nm, are enriched with active sites, stable over a wide range of pH and have great resistance to photobleaching. The N-CDs have bright blue emission with an improved quantum yield (QY) of up to 35.5%. The effect of the variables including pH, adsorbent mass, initial concentration and incubation time on the removal of Hg (II) was investigated using central composite design. The statistical results confirmed that the adsorption process could reach equilibrium within 30 min. The reduced cubic model (R² = 0.9989) revealed a good correlation between the observed values and predicted data. The optimal variables were pH of 7, dose of 0.1 g, initial concentration of 100 mg/L and duration of 30 min. Under these conditions, adsorption efficiency of 84.6% was obtained. The adsorption kinetic data could be well expressed by pseudo-second-order kinetic and Langmuir isotherm models. The optimal adsorption capacity was 116.3 mg g^-1. Furthermore, the adsorbent has a good selectivity towards Hg (II) with a detection limit of 0.01 μM due to the special interaction between Hg (II) and carboxyl/amino groups on the edge of N-CDs. This work provided an alternative direction for constructing low-cost adsorbents with effective sorption and sensing of Hg (II).

PMID:35802332 | DOI:10.1007/s11356-022-21844-0

Environ Sci Pollut Res Int. 2022 Jul 8. doi: 10.1007/s11356-022-21817-3. Online ahead of print.

ABSTRACT

Bisphenols are endocrine disruptor chemicals that disrupt thyroid hormone homeostasis. However, evidence on the effects of bisphenol mixtures on thyroid hormones are insufficient. Therefore, the present study aimed to explore the effects of bisphenol substitutes and bisphenol mixtures on thyroid hormones during pregnancy. The study was conducted among 446 pregnant women in the Guangxi Zhuang Birth Cohort (GZBC), China. In multiple linear regressions, compared with the low-exposure group, bisphenol S (BPS) concentrations in the middle-exposure group led to a 10.90% (95% CI: – 18.16%, – 2.99%) decrease in triiodothyronine (T3) levels in the first trimester; tetrabromobisphenol A (TBBPA) levels in the middle-exposure group led to an 8.26% (95% CI: – 15.82%, – 0.01%) decrease in T3 levels in the first trimester; bisphenol B (BPB) levels in the middle-exposure group led to higher free thyroxine (FT4) levels (9.84%; 95% CI: 1.73%, 18.60%) in the second trimester; bisphenol F (BPF) in the middle-exposure group led to higher FT4 levels (8.59%, 95% CI: 0.53%, 17.31%) in the second trimester; and TBBPA levels in the high-exposure group led to a 9.39% (95% CI: 1.46%, 17.93%) increase in FT4 levels in the second trimester. The Bayesian kernel machine regression (BKMR) and restricted cubic spline (RCS) models showed a U-shaped dose-response relationship between bisphenol A (BPA) and free triiodothyronine (FT3) (p < 0.01) as well as BPS and FT4 (p < 0.05). Nonlinear relationships were also observed between the bisphenol mixture and FT3. Overall, maternal bisphenol exposure affected thyroid hormone levels during pregnancy. This study provides evidence that BPB, BPF, BPS, and TBBPA are unsafe substitutes for BPA, as well as the overall effect of bisphenols on adverse health in human beings.

PMID:35802331 | DOI:10.1007/s11356-022-21817-3

Health Care Manag Sci. 2022 Jul 8. doi: 10.1007/s10729-022-09599-z. Online ahead of print.

ABSTRACT

Clinical pathways are standardized processes that outline the steps required for managing a specific disease. However, patient pathways often deviate from clinical pathways. Measuring the concordance of patient pathways to clinical pathways is important for health system monitoring and informing quality improvement initiatives. In this paper, we develop an inverse optimization-based approach to measuring pathway concordance in breast cancer, a complex disease. We capture this complexity in a hierarchical network that models the patient’s journey through the health system. A novel inverse shortest path model is formulated and solved on this hierarchical network to estimate arc costs, which are used to form a concordance metric to measure the distance between patient pathways and shortest paths (i.e., clinical pathways). Using real breast cancer patient data from Ontario, Canada, we demonstrate that our concordance metric has a statistically significant association with survival for all breast cancer patient subgroups. We also use it to quantify the extent of patient pathway discordances across all subgroups, finding that patients undertaking additional clinical activities constitute the primary driver of discordance in the population.

PMID:35802305 | DOI:10.1007/s10729-022-09599-z

Environ Sci Pollut Res Int. 2022 Jul 8. doi: 10.1007/s11356-022-21903-6. Online ahead of print.

ABSTRACT

In this research, diatoms as the first step and fishes as the last step of the food chain were compared as toxic metal accumulation bioindicator in an A-class wetland in Turkey. Bioaccumulations of potentially toxic elements (PTEs) were determined in liver, gill and muscle tissues of two commercially consumed fish species Carassius gibelio and Cyprinus carpio and in frustules of epiphytic diatom communities living on submerged macrophytes. Samples were collected seasonally from the Gala Lake, which is among the best stopover habitats of birds migrating between Europe and Africa, considering the paddy harvest period that is a major stress factor for the ecosystem. Also, potential human health risks associated with the consumption of fishes and consumption – dermal contact of diatoms were evaluated both for summer – before paddy harvest (BPH) and autumn – after paddy harvest (APH) periods. As a result of this research, the investigated toxic metal concentrations were increased significantly in diatoms in the APH period, while less significant exchanges were recorded in fishes. The bioaccumulations of PTEs were ranked as follows: Zn > Mn > Se > Cu > B > Cr > Ni > As > Pb > Cd for C. gibelio; Zn > Mn > Se > Cu > B > Cr > As > Ni > Pb > Cd for C. carpio; and Mn > Zn > Se > Pb > B > Ni > Cr > Cu > As > Cd for diatom frustules. Although the HI values in diatoms detected in the APH period were statistically significantly higher (about 1000 times; p < 0.05) than detected in the BPH period, they were less than the limit of 1 in both seasons. However, the HI coefficients of fishes were quite higher than the limit (an average of 23.59 for C. gibelio and 19.18 for C. carpio), which means quite high probable non-carcinogenic health risks for humans. Furthermore, the CR coefficients of Cr, Ni and As in muscle tissues of fishes were considerably higher than the limit of 10^-4, which reflects a significant carcinogenic health risk for consumers. The data showed that although the fishes at the top of the food chain bioaccumulate the PTEs in their tissues much higher than the diatoms at the bottom of the food chain, the diatoms are much more sensitive to changes in the environmental conditions than the fishes and they are more effective biological tools as toxic metal accumulation bioindicators.

PMID:35802324 | DOI:10.1007/s11356-022-21903-6

Inflammopharmacology. 2022 Jul 8. doi: 10.1007/s10787-022-01019-6. Online ahead of print.

ABSTRACT

BACKGROUND: Tumor necrosis factor inhibitor (TNFi) is recently reported to treat noninfectious uveitis (NIU) effectively. However, as a new kind of TNFi, golimumab is just on the market in China for several years, and its administration for NIU treatment lacks sufficient evidence. Therefore, the current study aimed to investigate the efficacy and safety of golimumab in refractory NIU patients.

METHODS: Thirty NIU patients with 49 affected eyes refractory to conventional treatments (corticosteroids and immunosuppressive agents) were consecutively enrolled. They received treatment of TNFi (50 mg golimumab every 4 weeks) for at least 6 months. The anterior chamber cell grade, vitreous haziness grade, central macular thickness, and visual acuity were evaluated at baseline, month (M) 1, M3, and M6.

RESULTS: After treatment, the anterior chamber cell grade declined from baseline (0.6 ± 0.7) to M6 (0.3 ± 0.5) (P < 0.001); the vitreous haziness grade decreased from baseline (1.2 ± 1.2) to M6 (0.4 ± 0.5) (P < 0.001); meanwhile, the central macular thickness also reduced from baseline (351.4 ± 90.8 μm) to M6 (271.8 ± 54.4 μm) (P < 0.001). In terms of visual acuity (LogMAR), it showed a declined trend from baseline (0.5 ± 0.3) to M6 (0.4 ± 0.2), but without statistical significance (P = 0.096). Subgroup analyses revealed that TNFi history related to decreased golimumab efficacy. In addition, 13.3% of patients had adverse events, including elevated liver enzymes (6.7%), fatigue (3.3%), and rash (3.3%).

CONCLUSION: Golimumab is effective and safe for refractory NIU treatment, while a large-scale trial is still needed for verification.

PMID:35802282 | DOI:10.1007/s10787-022-01019-6

J Clin Immunol. 2022 Jul 8. doi: 10.1007/s10875-022-01322-5. Online ahead of print.

NO ABSTRACT

PMID:35802274 | DOI:10.1007/s10875-022-01322-5

Pediatr Nephrol. 2022 Jul 8. doi: 10.1007/s00467-022-05674-3. Online ahead of print.

ABSTRACT

BACKGROUND: For the purpose of a better understanding of enteric hyperoxaluria in Crohn’s disease (CD) in children and adolescents, we investigated the occurrence and risk factors for development of hyperoxaluria in those patients.

METHODS: Forty-five children with CD and another 45 controls were involved in this cross-sectional study. Urine samples were collected for measurement of spot urine calcium/creatinine (Ur Ca/Cr), oxalate/creatinine (Ur Ox/Cr), and citrate/creatinine (Ur Citr/Cr) ratios. Fecal samples were also collected to detect the oxalyl-CoA decarboxylase of Oxalobacter formigenes by PCR. Patients were classified into 2 groups: group A (with hyperoxaluria) and group B (with normal urine oxalate excretion). The disease extent was assessed, and the activity index was calculated.

RESULTS: According to the activity index, 30 patients (66.7%) had mild disease and 13 patients (28.9%) had moderate disease. There was no significant difference in Ur Ox/Cr ratio regarding the disease activity index. O. formigenes was not detected in 91% of patients in group A while it was detected in all patients in group B (p < 0.001). By using logistic regression analysis, the overall model was statistically significant when compared to the null model, (χ² (7) = 52.19, p < 0.001), steatorrhea (p = 0.004), frequent stools (p = 0.009), and O. formigenes (p < 0.001).

CONCLUSION: Lack of intestinal colonization with O. formigenes, steatorrhea, and frequent stools are the main risk factors for development of enteric hyperoxaluria in CD patients. Identifying risk factors facilitates proper disease management in future studies. A higher resolution version of the Graphical abstract is available as Supplementary information.

PMID:35802269 | DOI:10.1007/s00467-022-05674-3

Eur J Orthop Surg Traumatol. 2022 Jul 8. doi: 10.1007/s00590-022-03326-8. Online ahead of print.

ABSTRACT

BACKGROUND: Outcomes data of intramedullary nail fixation (IMN) constructs for complex Schatzker VI tibial plateau fractures are scant in the literature. This study compares the clinical and radiographic outcomes of IMN, dual plate, and single plate constructs for Schatzker IV tibial plateau fractures.

METHODS: Retrospective cohort study of sixty-two patients at a University-based Level 1 trauma center who underwent open reduction internal fixation for Schatzker VI tibial plateau fracture. Constructs evaluated were IMN (with or without raft screws), dual plating, and single plating. Demographic, clinical, and radiographic outcomes were recorded. All fractures were additionally classified based on the OTA classification for sub analyses. Mean follow-up was 13.2 (SD 13.3) months. Predictors of construct selection and outcomes were evaluated with bivariate logistic regression. Outcomes were compared between groups with independent samples t-tests and Chi Square tests.

RESULTS: No significant demographic differences were found between IMN, dual plate or single plate construct cohorts. There was a higher proportion of open fractures within the IMN construct group versus the dual plate cohort (21.1% vs 3.6%). No statistically significant differences in radiographic outcomes were observed between cohort groups except for small but statistically significant differences in condylar width (CW) ratio change and tibial slope; when fracture cohorts were sub analyzed by specific OTA classification, there were no significant differences in any radiographic outcomes. There was a significant difference between the ratio of OTA 41C1, C2 and C3 fractures regarding treatment allocation (p = 0.004), favoring dual plate fixation for OTA 41C3 fractures. There were no significant differences found between treatment cohorts in terms of all cause complications (p > 0.05). IMN and single plate constructs were utilized when posteromedial condyle fractures were nondisplaced or minimally displaced.

CONCLUSION: Intramedullary nail fixation with or without supplemental raft screws produced similar short-term clinical and radiographic results compared to dual and single plate constructs among patients with Schatzker VI fracture types, regardless of OTA classification. Level of Evidence Level III retrospective cohort.

PMID:35802263 | DOI:10.1007/s00590-022-03326-8