Category: Statistics

Invest Ophthalmol Vis Sci. 2022 May 2;63(5):37. doi: 10.1167/iovs.63.5.37.

ABSTRACT

PURPOSE: To evaluate the potential causal associations between type 2 diabetes and fasting glucose and HbA1c levels and the risk of primary open-angle glaucoma (POAG) in European and East Asian populations.

METHODS: We selected genetic variants (P < 5 × 10-8) for type 2 diabetes (898,130 Europeans; 433,540 East Asians), fasting glucose, and HbA1c (196,991 Europeans; 36,584 East Asians) from three meta-analyses of genome-wide association studies (GWAS). The GWAS for POAG provided summary statistics (192,702 Europeans; 46,523 East Asians). Mendelian randomization (MR) analysis was accomplished using the inverse variance-weighted method, weighted-median method, MR Egger method, and MR-Pleiotropy RESidual Sum and Outlier test.

RESULTS: Genetically predicted type 2 diabetes was potentially positively associated with POAG in the European ancestry (body mass index [BMI]-unadjusted: odds ratio [OR] = 1.07, 95% confidence interval [CI], 1.01-1.14, P = 0.028; BMI-adjusted: OR = 1.07, 95% CI, 1.01-1.15, P = 0.035), but not in the East Asian ancestry (BMI-unadjusted: OR = 1.01, 95% CI, 0.95-1.06, P = 0.866; BMI-adjusted: OR = 1.00, 95% CI, 0.94-1.05, P = 0.882). There was no evidence to support a causal association of fasting glucose (European: OR = 1.19, P = 0.157; East Asian: OR = 0.94, P = 0.715) and HbA1c (European: OR = 1.27, P = 0.178; East Asian: OR = 0.85, P = 0.508) levels with POAG.

CONCLUSIONS: The causal effect of type 2 diabetes on the risk of POAG is different in European and East Asian populations. The point estimates of fasting glucose and Hb1Ac with POAG are large but not statistically significant, which prompts the question of statistical power.

PMID:35622353 | DOI:10.1167/iovs.63.5.37

Trop Med Int Health. 2022 May 27. doi: 10.1111/tmi.13782. Online ahead of print.

ABSTRACT

OBJECTIVE: To describe the prevalence of HIV viral suppression and assess the factors associated with HIV viral suppression among persons receiving ART in Malawi in 2021.

METHODS: Implementation study using routinely collected patient-level HIV RNA-PCR test result data extracted from the national Laboratory Management Information System (LIMS) database managed by the Department of HIV/AIDS in 2021. We calculated frequencies, proportions and odds ratios (OR) of HIV viral suppression with their associated 95% confidence intervals (95%CI). We performed a random-effects logistic regression to determine the risk factors associated with HIV viral suppression amongst ART patients, controlling for the spatial autocorrelation between districts and adjusting for other variables.

RESULTS: We evaluated 515,797 adults and children receiving ART and having a viral load test in 2021. Of these, 92.8% had HIV viral suppression. ART patients living in urban areas had lower likelihood of HIV viral suppression than those living in rural areas (adjusted OR [aOR]=0.95, 95%CI: 0.92-0.99, P=0.01). There was an increasing trend in HIV viral suppression with increasing ART duration. Routine VL monitoring samples were 39% more likely to have suppressed VL values than confirmatory HIV VL monitoring samples (aOR=1.39; 95%CI:1.34-1.43, P<0.001).

CONCLUSION: This is the first national analysis of Malawi HIV VL data from LIMS. Our findings show the need to particularly consider the urban residents, those below 20 years, males, those on ART for less than a year as well as those on specific ARV regimens in order to persistently suppress HIV VL and consequently achieve the goal of achieving HIV VL suppression by 2030.

PMID:35622358 | DOI:10.1111/tmi.13782

Acad Psychiatry. 2022 May 27. doi: 10.1007/s40596-022-01652-x. Online ahead of print.

ABSTRACT

OBJECTIVE: Despite the ongoing growing interest and established benefits of mental health recovery and peer support services, little is known about the effects of mental health recovery and peer support services specifically in community education programs. Seeking to further expand this scope, this article details the development, implementation, and evaluation of a recovery and peer services community psychoeducation program created by an academic psychiatrist and delivered by trained mental health peers.

METHODS: A community psychoeducation intervention on recovery and peer services was provided in 2 different series: 5-session and 1-session series. Participants in the intervention were recruited from 2 local nonprofit recovery-oriented organizations and a public mental health service agency. Surveys were administered before and after each presentation session and analyzed both quantitatively and qualitatively.

RESULTS: Quantitative survey items covering knowledge, awareness, interest, and likelihood of involvement in peer services for recovery all showed statistically significant gains from before to after the sessions. The qualitative data provided information about the participants’ interests in the program in terms of knowledge of recovery, self-improvement, advocacy, resources, support, and nonspecific positive comments.

CONCLUSIONS: An essential contribution of the program was to introduce participants to recovery and peer services. It also provided them with foundational knowledge that placed participants in a position to begin to practice the concepts of recovery. According to the participants’ comments in the surveys, the program imbued a sense of hope and motivation; support and fellowship; and, importantly, role modeling and wisdom from the presenters.

PMID:35622312 | DOI:10.1007/s40596-022-01652-x

Mol Biol Rep. 2022 May 27. doi: 10.1007/s11033-022-07583-y. Online ahead of print.

ABSTRACT

BACKGROUND: Cardiovascular diseases are the leading cause of death worldwide, with several conditions being affected by oxidative stress. Ferroptosis, recently identified programmed cell death mechanism, is relies on oxidative stress. This study aimed to determine the expressions of the genes involved in the molecular pathways of oxidative stress and ferroptosis and the association of these genes with CAD risk factors in CAD and non-CAD individuals.

METHODS AND RESULTS: The blood samples of individuals who underwent coronary angiography were collected and divided according to CAD status. Total RNA isolation was performed using the PAXgene RNA isolation kit from the whole blood samples. The mRNA expression levels of RTN3, GPX4, CAT, HMOX1, ELOVL5, SLC25A1, SLC7A11, and ACSL4 genes were determined using Real-Time PCR. Biochemical analyses were done before coronary angiography, and the results were evaluated statistically. The expression levels of the CAT gene are significantly lower in the CAD group when compared to non-CAD. HMOX1 expression levels are positively correlated with stenosis percentage, Gensini, and SYNTAX scores in the CAD group. RTN3, SLC25A1, and GPX4 mRNA expressions are correlated with HDL-C levels. Moreover, HbA1c levels and BMI, correlate negatively with ACSL4 expression in non-CAD controls. Also, ELOVL5 expression is negatively correlated with total bilirubin and direct bilirubin levels in the CAD group.

CONCLUSIONS: In this study, the genes related to oxidative stress and ferroptosis were found associated with biochemical parameters associated with CAD risk. These preliminary results may provide a new perspective to further studies investigating the reasons behind the identified associations.

PMID:35622307 | DOI:10.1007/s11033-022-07583-y

J Ultrasound. 2022 May 27. doi: 10.1007/s40477-022-00682-3. Online ahead of print.

ABSTRACT

INTRODUCTION: There is evolving literature on the superiority of transperineal ultrasound (TPU) in predicting the mode of delivery either before induction or during the course of labour. An ultrasound score (USG score) similar to a Bishop score or an ultrasound scoring method based on independent predictors before the onset of labour at term gestation would serve as a baseline objective record over which further progress of labour could be evaluated.

OBJECTIVE: 1. To sonographically examine the clinical parameters evaluated during a digital examination. 2. To derive an USG score using TPU parameters, similar to a Bishop score, and determine its performance in predicting pregnancy outcomes. 3. To identify which are the independent predictors of caesarean section among all the studied ultrasound (USG) parameters.

MATERIALS AND METHODS: In this prospective study, all the eligible women who came for routine antenatal care at term gestation and who were due for pelvic assessment were recruited. They underwent transabdominal ultrasound (TAS) for evaluating head position and head circumference, and TPU for evaluating head station with and without applying fundal pressure, various cervical parameters, sub pubic angle. Equivalent five USG parameters were scored along similar lines as the Bishop score. These women underwent vaginal examination for Bishop score and pelvic adequacy by an obstetrician who was blinded about USG parameters. While spontaneous onset of labour (SOL) was awaited in some of these women, some were induced as and when indicated. After delivery, the Bishop scores, pregnancy outcome in terms of SOL or induction of labour (IOL), mode of delivery and duration of labour were noted retrospectively from case records. Independent predictors of caesarean were obtained by univariate and multivariate analysis.

RESULTS: All the parameters were assessed for predictive ability of caesarean section by univariate analysis. The cervical length, angle of progression (AOP) at rest, head perineal distance (HPD) at rest and with pressure, Bishop score and USG score were found significant. On multivariate analysis, cervix length and USG score were found to be statistically significant. At a USG score of ≤ 5, the sensitivity and specificity to predict caesarean section were 84% and 68%, respectively. The relative risk (RR) was 4.9 (95% CI 2.3-12). The USG score could predict SOL. The p value was 0.001. The area under the curve (AUC) for the ultrasound score was 0.74.

CONCLUSIONS: USG examination by USG score at term gestation before the onset of labour can predict mode of delivery by caesarean section. It can predict spontaneous onset of labour. However, among all the individual parameters, cervix length has best predictive ability for caesarean section.

PMID:35622298 | DOI:10.1007/s40477-022-00682-3

Environ Sci Pollut Res Int. 2022 May 27. doi: 10.1007/s11356-022-20025-3. Online ahead of print.

ABSTRACT

Evidence on the effects of exposure to ambient gaseous pollutants on children’s vision was consistently scarce. We aimed to explore the effect of ambient gaseous pollutant exposure on the incidence of visual impairment (VI) in children. From 2005 to 2018, a total of 340,313 children without VI participated in a longitudinal and two-center dynamic cohort. The logMAR acuity was used to assess visual function. The space-time extremely randomized trees model was used to estimate SO₂ and CO exposures levels. The association between SO₂ and CO and VI risks among children was assessed using a proportional hazards model with a restricted cubic spline. Subgroup analyses stratified by gender and grades were used to investigate the differences in an association of SO₂ and CO exposures with childhood VI. A total of 158381 (46.54%) children experienced an new incident VI. A ten-unit (10 μg/m³) increase in SO₂ exposure concentrations was significantly associated with a 1.70 times higher risk of childhood VI. In addition, a 0.1-unit (0.1 mg/m³) increase in CO exposure was significantly associated with a 1.22 times higher risk of childhood VI. The positive association between ambient gaseous pollutants (including SO₂ and CO exposures) and childhood VI risks remained even after adjusting for other environmental variables. An increase in the incidence of VI in children was positively linked to SO₂ and CO exposure. Such evidence might aid governments in developing strategies to interfere with children’s eyesight by decreasing air pollution and changing school curricula.

PMID:35622291 | DOI:10.1007/s11356-022-20025-3

Environ Sci Pollut Res Int. 2022 May 27. doi: 10.1007/s11356-022-21042-y. Online ahead of print.

ABSTRACT

Understanding whether and how urban innovation offers a sound solution to the dilemma of urban green development is a crucial response to mitigate the detrimental effect on natural resources and environment for transitioning to sustainable urban development. To address the critical issue, we propose urban green development evaluation index system, and then examine how the urban innovation affects urban green development from the perspectives of government-scale, enterprise-scale, and spatial correlation network, all of which are originally applied in the 108 cities of Yangtze River Economic Belt of China (YREB) during period 2006-2018. The evaluation results show that urban innovation promotes urban green development, and both government-scale and enterprise-scale contribute to the effects. The constructed spatial correlation network of urban innovation illustrates the network structural form and reveals the network property, and further results tell that increasing network density and centrality would promote green development obviously. More specifically, the network density of urban innovation has been tied to the enhancement of urban green development, which is more significant in middle reaches than in lower and upper reaches of YREB. Similarly, optimizing the network’s degree centrality and closeness centrality can help facilitate urban green development in whole YREB. Thus, the research findings would provide new insights into the essence and driving forces from various scale and hidden network when exploring and seeking urban green development path.

PMID:35622287 | DOI:10.1007/s11356-022-21042-y

Brain Imaging Behav. 2022 May 27. doi: 10.1007/s11682-022-00674-1. Online ahead of print.

ABSTRACT

This study aimed to evaluate the differences of brain connectivity between healthy subjects (HS) and patients with extracranial internal carotid artery (eICA) stenosis before and after carotid endarterectomy (CEA). An exploratory prospective study was designed. The study population consisted of a patient group (PG) of 20 patients with eICA stenosis eligible for CEA, and a control group (CG) of 20 HS, matched for age and sex. The subjects of the PG group underwent Magnetic Resonance Imaging (MRI) for resting-state functional connectivity MRI (rs-fc MRI) analysis within one week from the CEA (pre-CEA) and 12 months following CEA (post-CEA). The CG underwent a single MRI with the same protocol utilized for the PG. Three region-of-interest to region-of-interest (ROI-to-ROI) rs-fc MRI analyses were conducted: analysis 1 to compare pre-CEA PG and CG; analysis 2 to compare pre-CEA PG and post-CEA PG; analysis 3 to compare post-CEA PG and CG. The Functional Network Connectivity multivariate parametric technique was used for statistical analysis, adopting a p-uncorrected (p-unc) < 0.05 as connection threshold, and a cluster level False Discovery Rate corrected p (p-FDR) < 0.05 as cluster threshold. The clusters were defined by using a data-driven hierarchical clustering procedure. Analysis 1 revealed two clusters of reduced interhemispheric connectivity of pre-CEA PG when compared to CG. Analysis 2 and 3 showed no statistically significant differences. Our exploratory analysis suggests that patients with eICA stenosis have reduced interhemispheric connectivity when compared to a matched control group, and this difference was not evident anymore following endarterectomy.

PMID:35622267 | DOI:10.1007/s11682-022-00674-1

Breast Cancer Res Treat. 2022 May 27. doi: 10.1007/s10549-022-06628-x. Online ahead of print.

ABSTRACT

INTRODUCTION: There is a paucity of literature comparing the postoperative outcomes of males and females with breast cancer who undergo mastectomy. The aim of this study is to evaluate the comorbidities and 30-day post-mastectomy complication rates among males and females.

METHODS: We performed a retrospective analysis of breast cancer patients who underwent mastectomy from 2014 to 2016 using the American College of Surgeon’s National Surgical Quality Improvement Project database. Data including patient demographics, comorbidities, and 30-day surgical and medical complications were collected. Statistical analysis included Chi-square and Fisher’s exact tests for categorical variables and Student T-tests for continuous variables. Statistical significance was defined as p < 0.05.

RESULTS: A total of 15,167 patients were identified. There were 497 males (3.3%) and 14,670 females (96.7%). Age was significantly higher in females compared to males (63.5 vs. 57.6 years, p < 0.001). Body mass index (BMI) at time of surgery was also higher in males (30.0 vs. 29.3 kg/m², p = 0.011). There was a higher prevalence of diabetes in males (20.1 vs. 16.5%, p = 0.032). Operative duration was significantly longer in females (114.9 vs. 95.0 min, p < 0.001). Median postoperative length of stay was also longer in females (1.2 vs. 0.8 days, p < 0.001). There were no significant differences in 30-day medical or surgical complication rates between the two sexes.

CONCLUSION: Our findings suggest that differences in age, BMI, and comorbidities between males and females do not significantly impact 30-day medical or surgical complications following total mastectomy for breast cancer. Further research is warranted to identify perioperative risk factors that influence post-mastectomy complication rates.

LEVEL OF EVIDENCE: 3 (Retrospective cohort study).

PMID:35622242 | DOI:10.1007/s10549-022-06628-x

Biometrics. 2022 May 27. doi: 10.1111/biom.13701. Online ahead of print.

ABSTRACT

With recent advances in technologies to profile multi-omics data at the single-cell level, integrative multi-omics data analysis has been increasingly popular. It is increasingly common that information such as methylation changes, chromatin accessibility, and gene expression are jointly collected in a single-cell experiment. In biomedical studies, it is often of interest to study the associations between various data types and to examine how these associations might change according to other factors such as cell types and gene regulatory components. However, since each data type usually has a distinct marginal distribution, joint analysis of these changes of associations using multi-omics data is statistically challenging. In this paper, we propose a flexible copula-based framework to model covariate-dependent correlation structures independent of their marginals. In addition, the proposed approach could jointly combine a wide variety of univariate marginal distributions, either discrete or continuous, including the class of zero-inflated distributions. The performance of the proposed framework is demonstrated through a series of simulation studies. Finally, it is applied to a set of experimental data to investigate the dynamic relationship between single-cell RNA-sequencing, chromatin accessibility, and DNA methylation at different germ layers during mouse gastrulation. This article is protected by copyright. All rights reserved.

PMID:35622236 | DOI:10.1111/biom.13701