Nevin Manimala

Haemophilia. 2026 Mar 4. doi: 10.1111/hae.70248. Online ahead of print.

ABSTRACT

INTRODUCTION: Hereditary plasminogen (PLG) deficiency is a rare congenital fibrinolytic disorder and a rare disease. This study conducted gene sequencing and statistical analysis on patients with cerebral infarction (CI) and decreased PLG activity (PLG:A) to explore the relationship between PLG gene variants and hereditary thrombotic diseases.

AIM: To analyse PLG gene variants in 18 patients with hereditary PLG deficiency and to investigate the associated clinical manifestations.

METHODS: This Retrospective Study Included 18 Patients With CI and Decreased PLG:A Who Were Admitted to the First Affiliated Hospital of Wenzhou Medical University from January 2021 to May 2025. The patients Ranged in Age from 16 to 70 years. Peripheral blood Samples Were Collected Before Treatment to Determine Relevant Coagulation Indicators Such As PLG:A, PLG antigen (PLG:Ag), protein C activity. Polymerase chain reaction (PCR) followed by direct sequencing was performed to analyse whole genome sequence of the PLG gene. Suspected variants were confirmed by reverse sequencing.

RESULTS: The 18 patients’ cranial magnetic resonance (MRI) revealed recent cerebral infarct lesions in all cases. PLG:A levels ranged from 19% to 67%. All patients were diagnosed with dysplasminogenemia. Genetic analysis identified four types of PLG gene variants: c.1858G > A (p.Ala620Thr), c.398A > G(p.His133Arg), c.2108G > A(p.Gly703Asp), and c.1702G > A (p.Gly568Arg). The p.Ala620Thr mutation was the most frequent, while p.His133Arg and p.Gly568Arg were newly identified variants.

CONCLUSION: Patients with hereditary PLG deficiency resulting from PLG gene variants are at a significantly increased risk of CI, potentially attributable to diminished PLG catalytic activity and consequent impairment of fibrinolytic function.

PMID:41778291 | DOI:10.1111/hae.70248

Conserv Physiol. 2026 Mar 2;14(1):coag013. doi: 10.1093/conphys/coag013. eCollection 2026.

ABSTRACT

To limit the impact of invasive predator species on the ecosystem of Floreana Island, Galapagos, a large-scale rodent eradication program was implemented. Due to the significant risk of secondary exposure to rodenticide, a mitigation tactic was used to protect the only native raptor found on Floreana, the Galapagos short-eared owl (Asio flammeus galapagoensis). A large proportion of the owl population was brought into human care in July 2023 with the goal to release back to the wild following the completion of the eradication program and reduction of the risk of exposure to rodenticides. During this time under human care, health examinations including blood collection for assessment of haematology and biochemistry parameters were completed. Descriptions of leukocyte morphology and population-based reference intervals (RI) are reported here utilizing results from 62 clinically healthy adults. Sex was determined using polymerase chain reaction, allowing for comparisons between males (n = 29) and females (n = 33). Statistically significant differences were found for several parameters: packed cell volume, relative and absolute monocyte counts, total protein, calcium and phosphorus levels were higher in females, while uric acid and glucose levels were significantly higher in males. These baseline RI are an important tool for assessment of this unique population while under human care, and will allow for comparisons and continued health monitoring after release back to the island of Floreana.

PMID:41778266 | PMC:PMC12952920 | DOI:10.1093/conphys/coag013

Pulm Circ. 2026 Mar 2;16(1):e70256. doi: 10.1002/pul2.70256. eCollection 2026 Jan.

ABSTRACT

In this first global study of patient perspectives in Group 2 and 3 PH, we demonstrate that those with Group 3 PH report significantly greater adverse impacts on physical and mental wellbeing than those in Group 2 or Group 1, despite a lower treatment side-effect profile.

PMID:41778265 | PMC:PMC12951357 | DOI:10.1002/pul2.70256

Pulm Circ. 2026 Mar 2;16(1):e70269. doi: 10.1002/pul2.70269. eCollection 2026 Jan.

ABSTRACT

The Pulmonary Hypertension Global Patient Survey (PH GPS) provides the first international examination of experiences among children with pulmonary hypertension (PH) and their caregivers. Through an online survey distributed via PH associations across 32 countries, we collected responses from 136 caregivers about diagnostic journeys, treatment experiences, healthcare access and research participation. Qualitative analysis revealed challenges around the ‘invisible’ nature of PH, with caregivers describing how schools and communities sometimes doubted their child’s limitations in the absence of visible symptoms, adding to family burden. Quantitative findings demonstrated extensive caregiver burden with 40% providing constant care while facing significant employment consequences, including leaving work altogether, reduced hours, or career changes. Although 78.8% of children accessed specialist centres, significant barriers persisted: 41.2% encountered diagnostic delays exceeding 6 months, 34.8% travelled over 2 h for appointments and 27.7% received no healthcare cost reimbursement. Treatment experiences revealed 37.7% of children experienced side effects, yet only one-third discussed changes with their clinical teams, reflecting pragmatic decisions within limited therapeutic options. Research participation was low (19.2%), though families with trial experience showed greater willingness for future participation, compared to those without experience (70.8% vs. 49.5%). Barriers centred on awareness gaps (16.4%), safety concerns (14.5%), and age-related considerations (10.9%), distinct from adult PH populations where logistical challenges predominate. These findings demonstrate that paediatric care must extend beyond medical management to address family needs including social-work assistance for benefits navigation, flexible care delivery models incorporating telemedicine, pro-active communication about treatment tolerability and research protocols designed with families from inception.

PMID:41778264 | PMC:PMC12951355 | DOI:10.1002/pul2.70269

Pulm Circ. 2026 Mar 2;16(1):e70264. doi: 10.1002/pul2.70264. eCollection 2026 Jan.

ABSTRACT

The Pulmonary Hypertension (PH) Global Patient Survey provides the first truly international description of the symptomatic burden of PH and its treatment on patient’s health-related quality of life (HRQoL). From 3329 adult responses across 88 countries, the “invisible” nature of PH is an over-arching theme, with repercussions on socialisation, relationships and employment. Emotional burden is common with 35.6% feeling misunderstood, 33.4% angry or frustrated and 32.0% isolated. A reduced capacity for paid employment, in combination with healthcare/medication costs and difficulty in accessing social and financial support, compound societal exclusion and isolation. Physical limitations are almost universally reported (78.6%) with poor sleep quality (42.3%) the greatest factor in physical well-being. Treatment side-effects are common (46.7%), are greatest with parenteral therapies, but improve with age. Women of child-bearing age bear the brunt of PH physical and psychological burden, compounded by inconsistent messages from specialist services surrounding pregnancy and contraception. Patient-reported outcome measures (PROM) accurately reflect impacts from PH but are underutilised in clinical practice. Many respondents would like to be better able to discuss their emotional symptoms with their PH specialist (but often find a lack of empathy). Psychological support is a highly requested, but frequently unmet, need reinforcing the importance of holistic care models and multiagency working in managing patients with PH.

PMID:41778263 | PMC:PMC12951358 | DOI:10.1002/pul2.70264

MethodsX. 2026 Feb 20;16:103840. doi: 10.1016/j.mex.2026.103840. eCollection 2026 Jun.

ABSTRACT

Dermatophytosis is an emerging public health concern in developing countries, with rising infection rates and persistent disease burden. Reliable isolation of dermatophytes is essential for effective treatment, surveillance, and downstream analyses, yet it remains challenging in low-resource clinical settings. We evaluated three mycological sampling approaches, including one conventional and two modified techniques to improve the recovery of pure dermatophytes while reducing contamination. The modified approaches comprised a heat-assisted aseptic sampling method to limit airborne contamination and a paper-zip transport approach using heat and sterile filter paper sealed in zip-lock bags before inoculation into the culture medium. The outcomes of all sampling approaches were subsequently assessed using conventional culture techniques. A total of 198 clinical specimens from 66 individuals generated 276 culture outcomes. Statistical analysis showed significant differences among methods in recovery of pure isolates and contamination control, with the Paper-Zip method exhibiting the highest selectivity (p < 0.05). Sensitivity analysis using conventional culture techniques showed similar diagnostic performance across the approaches, reflecting improvements in dermatophytes recovery. Heat-assisted and Paper-Zip sampling improve recovery of pure dermatophytes while minimizing contamination. Diagnostic sensitivity is consistent across all sampling approaches. The study demonstrates practical, low-cost implementable options for routine diagnostics in resource-limited clinical settings.

PMID:41778257 | PMC:PMC12950414 | DOI:10.1016/j.mex.2026.103840

MethodsX. 2026 Feb 20;16:103838. doi: 10.1016/j.mex.2026.103838. eCollection 2026 Jun.

ABSTRACT

Plants are known to generate various types of electrical signals, which have been observed ever since Darwin’s times. We studied the electrical signals acquired in tomato plants infected with the fungal pathogen Oidium neolycopersici (On), the causative agent of powdery mildew, and applied statistical analyses to detect the differences in electrical responses between healthy and infected plants, as reported in [1].•The underlying mechanism in the generation and transmission of electrical signals is not fully understood, yet it’s generally accepted that they can be classified according to functional properties. Action potentials (APs) and slow wave potentials, in particular, are elicited by biotic and abiotic stimuli, thus are interesting as a hallmark of plant health status.•To analyse the application of these potentials in plant disease detection, voltages from electrodes inserted in plants were acquired periodically by a scanning multimeter and recorded under control of a dedicated custom Python program running on a Raspberry Pi board.•Here we describe the design of the experiment and analyse in some detail the solutions adopted for specific issues found in the measurements, such as electrode’s material and placement; immunity to electromagnetic noise; data logging over long periods of time with intermediate monitoring of results.

PMID:41778256 | PMC:PMC12950449 | DOI:10.1016/j.mex.2026.103838

Cancer Diagn Progn. 2026 Mar 1;6(2):291-302. doi: 10.21873/cdp.10528. eCollection 2026 Mar-Apr.

ABSTRACT

BACKGROUND/AIM: Intraductal papillary mucinous neoplasm (IPMN) of the pancreas is a precursor lesion with variable malignant potential. Due to its heterogeneity, optimal treatment strategies remain controversial, especially regarding surgical resection and surveillance indications. We reviewed our institutional outcomes to reassess the current postoperative strategy and refine management guidelines.

PATIENTS AND METHODS: This study retrospectively and consecutively analyzed the data of 49 IPMN patients who underwent pancreatectomy at an academic institution from 2015 to May 2025.

RESULTS: Diagnostic mismatch between preoperative and final pathological findings was observed in 39% of cases, with overdiagnosis (downgrade group) being more common than underdiagnosis. Overdiagnosed cases were significantly associated with main pancreatic duct dilation (>5 mm) (p=0.012) and elevated amylase levels (p=0.031), while the only upgraded case involved invasive carcinoma with mural nodule and Sonazoid enhancement. Histological grade strongly influenced prognosis: Patients with adenoma or carcinoma in situ showed favorable outcomes (5-year OS ≥89%), whereas those with invasive IPMN had markedly worse survival (5-year OS 36%; p<0.001). Elevated CA19-9 was a significant negative prognostic factor (p=0.031), while lymph node metastasis (p=0.035) and advanced tumor stage (p=0.0014) were also associated with poor outcomes. Tumors located in the pancreatic tail and those classified as mixed-type IPMN tended to have inferior survival, though without statistical significance. Cancer recurrence occurred in 18% of patients, primarily via peritoneal and hepatic routes.

CONCLUSION: Preoperative diagnostic inaccuracies remain common in IPMN, and invasive transformation, elevated CA19-9, lymph node metastasis, and tumor stage are key prognostic factors. A multimodal diagnostic approach is needed to improve risk stratification and guide appropriate surgical management.

PMID:41778248 | PMC:PMC12951388 | DOI:10.21873/cdp.10528

Cancer Diagn Progn. 2026 Mar 1;6(2):199-213. doi: 10.21873/cdp.10519. eCollection 2026 Mar-Apr.

ABSTRACT

BACKGROUND/AIM: Lung cancer is one of the leading causes of cancer deaths. While low-dose computed tomography (CT) screening improves survival, radiological detection is increasingly challenged by a shortage of radiologists. This study aimed to develop and evaluate a novel, precise, and computationally efficient AI-based algorithm for lung cancer diagnosis using chest CT scans.

PATIENTS AND METHODS: A total of 156 patient chest CT scans were utilized to form Databases I and II. We then conducted extensive feature extraction [statistics, histograms, Fast Fourier Transform (FFT), Discrete Cosine Transform (DCT), Walsh-Hadamard Transform (WHT)] and optimized classifiers [Multi Layer Perceptron (MLP), Generalized Feed Forward Neural Network (GFF-NN), Modular Neural Network (MNN), Support Vector Machine (SVM)] with genetic algorithms. Performance evaluation measures employed were classification accuracy, Mean Squared Error (MSE), Area under the ROC curve (AUC), and computational efficiency.

RESULTS: The MNN (Topology II) classifier employing FFT-based features with momentum learning achieved 100% classification accuracy during cross-validation for both Database I and Database II, consistently yielding perfect average classification accuracy across both datasets.

CONCLUSION: The genetically optimized MNN (Topology II) classifier shows remarkable performance in lung cancer diagnosis from CT scan images. Its ability to achieve perfect classification accuracy suggests strong potential for clinical application, offering both diagnostic precision, acting as a triage, and workload reduction in healthcare settings.

PMID:41778236 | PMC:PMC12951378 | DOI:10.21873/cdp.10519

Neurol Res Int. 2026 Mar 2;2026:8882884. doi: 10.1155/nri/8882884. eCollection 2026.

ABSTRACT

BACKGROUND: Normal pressure hydrocephalus (NPH) is a neurological disorder in older adults, characterized by gait disturbance, urinary incontinence, and cognitive impairment, along with ventriculomegaly and normal intracranial pressure. The management of NPH often involves ventriculoperitoneal shunting (VPS), which can be programmable (P-VPS) or nonprogrammable (NP-VPS). While P-VPS offers the advantage of adjustable pressure settings, its impact on clinical outcomes and complications remains debated, particularly in resource-limited settings like Jordan.

METHOD: A retrospective review was conducted of 38 adult patients diagnosed with idiopathic NPH who underwent VPS placement between 2018 and 2024. Patients were classified into two groups: P-VPS and NP-VPS. Clinical outcomes, including symptom improvement, complication rates, hospital stay duration, and shunt revisions, were analyzed. Statistical comparisons were made using SPSS, with p values < 0.05 considered significant.

RESULTS: The study found no significant differences between the two groups in symptom improvement. However, the NP-VPS group had a significantly shorter hospital stay (5.7 ± 3.2 days vs. 14.1 ± 11.9 days, p = 0.007). Complication rates, including infection and shunt revision, were higher in the P-VPS group (20.0% vs. 7.7% for infection; 32.0% vs. 15.4% for revision), though differences were not statistically significant.

CONCLUSION: Both P-VPS and NP-VPS resulted in similar symptom improvements, with NP-VPS showing a trend toward shorter hospital stays and comparable complication rates. Further multicenter studies with larger sample sizes are needed to validate these findings and refine management strategies for NPH.

PMID:41778232 | PMC:PMC12951206 | DOI:10.1155/nri/8882884