Nevin Manimala

Cell Mol Biol (Noisy-le-grand). 2023 May 31;69(5):75-79. doi: 10.14715/cmb/2023.69.5.13.

ABSTRACT

Diabetes is caused by peripheral insulin resistance and lack of insulin secretion due to the apoptosis of pancreatic beta cells. Tumor necrosis factor alpha (TNF-α), a pro-inflammatory cytokine secreted from the tissue on the insulin signaling pathway, can play a role in causing fat resistance to insulin in type 2 diabetes patients. Adiponectin is a specific protein of adipose tissue. It belongs to the collectin family, which is present in human plasma at a high level and can protect against vascular lesions. Considering the importance of epigenetic changes in the development of multifactorial diseases, this study was conducted to investigate the methylation of TNF-α gene promoter in patients with type diabetes with cardiovascular disease and compare it with diabetic people without cardiovascular disease. Also, the serum concentration of adiponectin was investigated in diabetic patients with and without cardiovascular disease. For this purpose, 95 patients with type 2 diabetes referred to Isfahan Endocrine and Metabolism Research Center were divided into two groups: cardiovascular disease and without cardiovascular disease, based on the angiography results. Serum adiponectin level was measured by the RIA method. In addition to adiponectin, indicators such as FBS, cholesterol, triglycerides, and HDL were also measured in these patients. Then, the promoter region of the TNF-α gene was investigated by bisulfite treatment method, nested PCR, and finally, sequence determination. The results showed that the serum level of adiponectin was higher in diabetic patients without cardiovascular disease than in diabetic patients with cardiovascular disease, but this difference was not statistically significant. Also, no change was observed between men and women in TNF-α gene promoter methylation in diabetic and non-diabetic groups. In general, the decrease in adiponectin concentration in diabetic people can be a factor in causing macroangiopathy, so it can be predicted that the production of recombinant adiponectin can be helpful in the treatment and protection of cardiovascular disease in these patients. Also, it seems that the epigenetic changes of cytokines that play a role in causing insulin resistance in type 2 diabetic patients are not noticeable in the peripheral blood sample. In this regard, other tissues should probably be investigated.

PMID:37571897 | DOI:10.14715/cmb/2023.69.5.13

Allergy. 2023 Aug 12. doi: 10.1111/all.15837. Online ahead of print.

ABSTRACT

BACKGROUND: Viruses may drive immune mechanisms responsible for chronic rhinosinusitis with nasal polyposis (CRSwNP), but little is known about the underlying molecular mechanisms.

OBJECTIVES: To identify epigenetic and transcriptional responses to a common upper respiratory pathogen, rhinovirus (RV), that are specific to patients with CRSwNP using a primary sinonasal epithelial cell culture model.

METHODS: Airway epithelial cells were collected at surgery from patients with CRSwNP (cases) and from controls without sinus disease, cultured, and then exposed to RV or vehicle for 48 h. Differential gene expression and DNA methylation (DNAm) between cases and controls in response to RV were determined using linear mixed models. Weighted gene co-expression analysis (WGCNA) was used to identify (a) co-regulated gene expression and DNAm signatures, and (b) genes, pathways, and regulatory mechanisms specific to CRSwNP.

RESULTS: We identified 5585 differential transcriptional and 261 DNAm responses (FDR <0.10) to RV between CRSwNP cases and controls. These differential responses formed three co-expression/co-methylation modules that were related to CRSwNP and three that were related to RV (Bonferroni corrected p < .01). Most (95%) of the differentially methylated CpGs (DMCs) were in modules related to CRSwNP, whereas the differentially expressed genes (DEGs) were more equally distributed between the CRSwNP- and RV-related modules. Genes in the CRSwNP-related modules were enriched in known CRS and/or viral response immune pathways.

CONCLUSION: RV activates specific epigenetic programs and correlated transcriptional networks in the sinonasal epithelium of individuals with CRSwNP. These novel observations suggest epigenetic signatures specific to patients with CRSwNP modulate response to viral pathogens at the mucosal environmental interface. Determining how viral response pathways are involved in epithelial inflammation in CRSwNP could lead to therapeutic targets for this burdensome airway disorder.

PMID:37571876 | DOI:10.1111/all.15837

J Hum Lact. 2023 Aug 11:8903344231190625. doi: 10.1177/08903344231190625. Online ahead of print.

ABSTRACT

BACKGROUND: Breastfeeding confers significant maternal and infant benefits; however, breastfeeding rates remain suboptimal in the United States. A parent’s decision to breastfeed is influenced by non-modifiable and modifiable factors, including breastfeeding knowledge and self-efficacy. There is a positive correlation between high maternal self-efficacy and breastfeeding duration. Parents increasingly rely on technology for health information.

RESEARCH AIM: To determine if a smartphone application affected maternal self-efficacy and breastfeeding exclusivity rates.

METHOD: This study was a randomized, controlled pilot study examining the effect of an educational program, included in a smartphone application, on breastfeeding self-efficacy (assessed in postpartum Week 1 and Weeks 4-6) and breastfeeding rates (assessed in postpartum Weeks 4-6). Forty participants were recruited using block randomization to intervention (17 of 20 completed the study) and usual care (19 of 20 completed the study) groups. To examine the pre-test/post-test difference in the Breastfeeding Self-Efficacy Scale – Short Form total scores, a change in score (post-intervention minus pre-intervention) was calculated for each parent.

RESULT: The intervention group (phone application and usual care) showed greater change in self-efficacy scores (M = 7.6, SD = 7.8) compared to the control group (usual care; M = 1.2, SD = 3.7, p = .001). The rate of exclusive breastfeeding was nearly twice as high in the intervention group as in the control group, but did not reach statistical significance (p = .093).

CONCLUSION: The investigators found enhanced breastfeeding self-efficacy and breastfeeding rates among postpartum women receiving a smartphone educational program in the first 6 weeks postpartum. Further studies on smartphone interventions will develop our understanding of this technology in improving breastfeeding rates.

PMID:37571838 | DOI:10.1177/08903344231190625

Ann Noninvasive Electrocardiol. 2023 Aug 11:e13082. doi: 10.1111/anec.13082. Online ahead of print.

ABSTRACT

BACKGROUND: To evaluate the diagnostic utility of basal and post-exercise QRS-T angle in patients with stable coronary artery disease (CAD).

METHODS: This cross-sectional and observational study analyzed 190 patients with stable angina. The QRS-T angle is measured on the 12-lead electrocardiograms at baseline and just after stopping the treadmill stress test in patients undergoing conventional coronary angiography (CAG). The pre- and post-exercise QRS-T angle and ΔQRS-T angle were analyzed.

RESULTS: Of the 190 patients, 66 (34.7%) were assigned to group 1 (patients with coronary lesion) and 124 (65.3%) to group 2 (patients without coronary lesion) after CAG. There was no statistically significant difference in QRS-T angle between groups at baseline (pre-exercise) (30.7 ± 17 vs. 27.8 ± 12.8, p = .233). The QRS-T angle value was significantly higher in group 1 than in group 2 (68.8 ± 40.3 vs. 22.7 ± 21.5, p = .01) after exercise (post-exercise). The ΔQRS-T angle was also significantly higher in group 1 than in group 2 (38.1 ± 37.6 vs. -5.1 ± 22.9, p = .01). Receiver operating characteristic curve revealed that the cut-off value of QRS-T angle (post-exercise) for the coronary obstruction was >51.5° with 81% of sensitivity and 66% of specificity (AUC: 0.832, p = .001, CI: 0.769-0.894). Duke treadmill score for coronary stenosis was >1.5 with 77% of sensitivity and 69% of specificity (AUC: 0.814, p = .001, CI: 0.749-0.878).

CONCLUSION: It could be proposed that post-exercise QRS-T angle and Δ QRS-T angle are significantly associated with coronary obstruction in patients with stable angina and appear to be more sensitive than the Duke treadmill score and traditional electrocardiographic parameters.

PMID:37571836 | DOI:10.1111/anec.13082

J Cutan Med Surg. 2023 Aug 11:12034754231191509. doi: 10.1177/12034754231191509. Online ahead of print.

ABSTRACT

BACKGROUND: The Psoriasis Longitudinal Assessment and Registry (PSOLAR) is a global, prospective, longitudinal, disease-based registry. It serves as a post-marketing safety commitment with a focus on patients with moderate to severe plaque psoriasis who are candidates for systemic therapy.

OBJECTIVES: To describe the baseline disease demographics and clinical characteristics of a Canadian subgroup of participants enrolled in PSOLAR.

METHODS: Baseline demographic/disease characteristics, medical histories, and previous psoriasis treatments for Canadian patients in PSOLAR were summarized using descriptive statistics.

RESULTS: There were 1896 patients analyzed in the Canadian subgroup at 37 clinical sites, accounting for 15.7% of the global PSOLAR population. Baseline disease and clinical characteristics were as expected for a moderate to severe psoriasis population and were generally similar to the global PSOLAR population. Two distinctions were noted in the Canadian subgroup versus those enrolled globally: a higher proportion of patients were overweight/obese (84.7% vs. 80.4%) and male (61.4% vs. 54.7%). In addition, the Canadian subgroup had numerically higher historical peak disease activity (PGA score 3.35 vs. 3.1) and longer disease duration (22.3 years vs. 17.5 years). Canadian PSOLAR patients reported a variety of comorbidities, including psoriatic arthritis (31.5%), hypertension (34.6%), hyperlipidemia (24.3%), mental illness (24.1%), and inflammatory bowel disease (1.6%).

CONCLUSION: The Canadian subgroup of PSOLAR patients was generally similar to those enrolled globally with respect to baseline disease demographics and clinical characteristics. Multiple comorbidities are noted in the Canadian subgroup, underscoring the need for a holistic approach to the treatment of psoriatic patients.

PMID:37571829 | DOI:10.1177/12034754231191509

Sensors (Basel). 2023 Aug 7;23(15):7010. doi: 10.3390/s23157010.

ABSTRACT

It is known that X-ray and gamma-ray pulsars can only be observed by spacecraft because signals from these pulsars are impossible to be detected on the Earth’s surface due to their strong absorption by the Earth’s atmosphere. The article is devoted to the theoretical aspects regarding the development of an autonomous radio navigation system for transport with a small receiving antenna, using radio signals from pulsars, similar to navigation systems for space navigation. Like GNSS systems (X-ray and radio), they use signals from four suitable pulsars to position the object. These radio pulsars (out of 50) are not uniformly distributed but are grouped in certain directions (at least 6 clusters can be determined). When using small antennas (with an area of up to tens of square meters) for pulsar navigation, the energy of the pulsar signals received within a few minutes is extremely insufficient to obtain the required level of SNR at the output of the receiver to form TOA estimation, ensuring positioning accuracy up to tens of kilometers. This is one of the scientific tasks that is solved in the paper by studying the relationship between the SNR of the receiver output, which depends on the size of the antenna, the type of signal processing, and the magnitude of the TOA accuracy estimate. The second scientific task that is solved in the paper is the adaptation of all the possible approaches and algorithms suggested in the statistical theory of radars in the suggested signal algorithm for antenna processing and to evaluate the parameters of the TOA and DS pulsar signals, in order to increase the SNR ratio at the receiver output, while preserving the dimensions of the antenna. In this paper, the functional structure of signal processing in a pulsar transport navigation system is proposed, and the choice of the observed second and millisecond pulsars for obtaining a more accurate TOA estimate is discussed. The proposed estimates of positioning accuracy (TOA only, no phase) in an autonomous pulsar vehicle navigation system would only be suitable for the navigation of large vehicles (sea, air, or land) that do not require accurate navigation at sea, air, or desert. Large-sized antennas with an area of tens of square meters to hundreds of square meters can be installed in such vehicles.

PMID:37571794 | DOI:10.3390/s23157010

Sensors (Basel). 2023 Aug 5;23(15):6968. doi: 10.3390/s23156968.

ABSTRACT

Under the influence of multiple types of noises, missing measurement, one-step measurement delay and packet loss, the robust Kalman estimation problem is studied for the multi-sensor descriptor system (MSDS) in this paper. Moreover, the established MSDS model describes uncertain-variance noises, multiplicative noises, time delay and packet loss phenomena. Different types of noises and packet loss make it more difficult to build the estimators of MSDS. Firstly, MSDS is transformed to the new system model by applying the singular value decomposition (SVD) method, augmented state and fictitious noise approach. Furthermore, the robust Kalman estimator is constructed for the newly deduced augmented system based on the min-max robust estimation principle and Kalman filter theory. In addition, the given estimator consists of four parts, which are the usual Kalman filter, predictor, smoother and white noise deconvolution estimator. Then, the robust fusion Kalman estimator is obtained for MSDS according to the relation of augmented state and the original system state. Simultaneously, the robustness is demonstrated for the actual Kalman estimator of MSDS by using the mathematical induction method and Lyapunov’s equation. Furthermore, the error variance of the obtained Kalman estimator is guaranteed to the upper bound for all admissible uncertain noise variance. Finally, the simulation example of a circuit system is examined to illustrate the performance and effectiveness of the robust estimators.

PMID:37571750 | DOI:10.3390/s23156968

Biomater Adv. 2023 Jul 28;153:213572. doi: 10.1016/j.bioadv.2023.213572. Online ahead of print.

ABSTRACT

Data-enabled approaches that complement experimental testing offer new capabilities to investigate the interplay between chemical, physical and mechanical attributes of alloys and elucidate their effect on biological behaviours. Reported here, instead of physical causation, statistical correlations were used to study the factors responsible for the adhesion, proliferation and maturation of pre-osteoblasts MC3T3-E1 cultured on Titanium alloys. Eight alloys with varying wt% of Niobium, Zirconium, Tin and Tantalum (Ti- (2-22 wt%)Nb- (5-20 wt%)Zr- (0-18 wt%)Sn- (0-14 wt%)Ta) were designed to achieve exemplars of allotropes (incl., metastable-β, β + α’, α″). Following confirmation of their compositions (ICP, EDX) and their crystal structure (XRD, SEM), their compressive bulk properties were measured and their surface features characterised (XPS, SFE). Because these alloys are intended for the manufacture of implantable orthopaedic devices, the correlation focuses on the effect of surface properties on cellular behaviour. Physico-chemical attributes were paired to biological performance, and these highlight the positive interdependencies between oxide composition and proliferation (esp. Ti⁴⁺), and maturation (esp. Zr⁴⁺). The correlation reveals the negative effect of oxide thickness, esp. TiO_x and TaO_x on osteoblastogenesis. This study also shows that the characterisation of the chemical state and elemental electronic structure of the alloys’ surface is more predictive than physical properties, namely SFE and roughness.

PMID:37566936 | DOI:10.1016/j.bioadv.2023.213572

Am J Speech Lang Pathol. 2023 Aug 11:1-17. doi: 10.1044/2023_AJSLP-22-00338. Online ahead of print.

ABSTRACT

PURPOSE: Exercise-based treatments may improve swallowing safety and efficiency; yet, it is not clearly understood which factors predict nonadherence to recommended treatment protocols. The aim of this study was to construct an algorithm for stratifying risk of nonadherence to a lingual strengthening dysphagia treatment program.

METHOD: Using recursive partitioning, we created a classification tree built from a pool of sociodemographic, clinical, and functional status indicators to identify risk groups for nonadherence to an intensive lingual strengthening treatment program. Nonadherence, or noncompletion, was defined as not completing two or more follow-up sessions or a final session within 84 days.

RESULTS: The study cohort consisted of 243 Veterans enrolled in the Intensive Dysphagia Treatment program across six sites from January 2012 to August 2019. The overall rate of nonadherence in this cohort was 38%. The classification tree demonstrated good discriminate validity (C-statistic = 0.74) and contained eight groups from five variables: primary diagnosis, marital status, Penetration-Aspiration Scale (PAS) severity score, race/ethnicity, and age. Nonadherence risk was categorized as high (range: 69%-77%), intermediate (27%-33%), and low risk (≤ 13%-22%).

CONCLUSIONS: This study identified distinct risk groups for nonadherence to lingual strengthening dysphagia rehabilitation. Additional research is necessary to understand how these factors may drive nonadherence. With external validation and refinement through prospective studies, a clinically relevant risk stratification tool can be developed to identify patients who may be at high risk for nonadherence and provide targeted patient support to mitigate risk and provide for unmet needs.

PMID:37566883 | DOI:10.1044/2023_AJSLP-22-00338

J Glaucoma. 2023 Aug 1. doi: 10.1097/IJG.0000000000002280. Online ahead of print.

ABSTRACT

PRECIS: Primary angle closure and primary angle-closure glaucoma may exhibit normal intraocular pressure. Twenty-four-hour intraocular pressure fluctuation is highest in primary angle-closure glaucoma. The degree of peripheral anterior synechiae was associated with 24-hour intraocular pressure pattern in primary angle-closure disease without laser iridotomy.

PURPOSE: To study 24-hour intraocular pressure (IOP) patterns in eyes with chronic primary angle-closure disease (PACD) and evaluate associations between peripheral anterior synechiae (PAS) and 24-hour IOP pattern.

PATIENTS AND METHODS: Prospective cohort study. Fifty-nine eyes of 35 Asian patients with chronic PACD underwent complete ocular examinations at Ramathibodi Hospital, Mahidol University. Twenty-four-hour IOP records were obtained using Goldmann applanation tonometry at 2-hour intervals. Peak, mean, and trough 24-hour IOP values and 24-hour IOP fluctuation (difference between peak and trough values) were compared among groups. None of the participants received any treatment before complete data collection.

RESULTS: Even univariable analysis demonstrated a significant difference in peak, mean, and trough IOP and 24-hour IOP fluctuation between the three groups, the magnitude of trough IOP was not higher than 21 mmHg in all groups. In multivariable analysis, PAC and PACG eyes showed significantly higher peak IOP (P=0.020 and P=0.006, respectively) and 24-hour IOP fluctuation (P=0.048 and P=0.001, respectively) compared with PACS eyes. In comparison between combined PACS and PAC eyes versus PACG eyes, PACG eyes revealed significantly higher 24-hour IOP fluctuation. The degree of PAS was associated with peak and mean IOP values and with 24-hour IOP fluctuation in PAC and PACG eyes.

CONCLUSIONS: Twenty-four-hour IOP fluctuation was highest in PACG eyes. Although PAC and PACG eyes showed higher peak IOP and 24-hour IOP fluctuation values, compared with PACS eyes, trough IOP in PAC and PACG eyes were mostly below 21 mmHg. In addition, the degree of PAS was associated with a 24-hour IOP pattern in either PAC or PACG eyes.

PMID:37566875 | DOI:10.1097/IJG.0000000000002280