Nevin Manimala

Zhonghua Shao Shang Za Zhi. 2021 Nov 23;37:1-9. doi: 10.3760/cma.j.cn501120-20200914-00409. Online ahead of print.

ABSTRACT

Objective: To investigate the effect of N-trimethyl chitosan-recombinant tissue factor pathway inhibitor (rTFPI) complex on avulsion flap with roll compaction in rat. Methods: The experimental methods were adopted. The N-trimethyl chitosan-rTFPI complex solution was prepared by ion cross-linking method. The morphological distribution of the complex was observed by scanning electron microscope, and its diameter was measured. The encapsulation rate and drug loading rate of rTFPI in the complex was determined and calculated by enzyme-linked immunosorbent assay (ELISA) method (n=3). The concentration of rTFPI in the solution at 0, 10, 30, 45, 60, 90, 120, 240 minutes of storage was measured by ELISA method to observe the release of rTFPI, and its half-life was calculated (n=3). Twenty-four 6-week-old male Sprague-Dawley rats were divided into phosphate buffered saline (PBS) group, N-trimethyl chitosan alone group, rTFPI alone group, and N-trimethyl chitosan-rTFPI group according to the random number table, with 6 rats in each group. The avulsion flaps with roll compaction were prepared on the backs of rats with pedicles located on the line of the bilateral iliac spine and lifted from the surface of the muscle membrane. One injection of corresponding reagents was injected once in situ sutures were made and on post operation day (POD) 1, 2, and 3. General changes of the flap were observed on POD 1, 3, and 7 before the injection in the same day. On POD 7, the survival area of the flap was measured and the survival rate of the flap was calculated; the flaps were divided into pedicle, proximal, middle, and distal segments, and the blood perfusion in the proximal, middle, and distal segment tissue of the flap was detected by the laser speckle blood flow imager; tissue samples in the middle of the flap were cut and stained with hematoxylin and eosin to observe the changes in tissue structure and the infiltration of inflammatory cells, and the numbers of embolized blood vessels and new blood vessels per 100 times visual field were counted. Data were statistically analyzed with one-way analysis of variance and least significant difference test. Results: The N-trimethyl chitosan-rTFPI complex had an irregular spherical structure with a diameter of 150-200 nm. The encapsulation rate and drug loading rate of rTFPI in the complex were (88.7±2.1)% and (2.83±0.09)%, respectively. The concentration of rTFPI in the solution of the N-trimethyl chitosan-rTFPI complex gradually increased with prolonged storage time, and the release was basically stable at 90 min, with half-life of (651±36) min. On POD 1, the distal parts of flaps of rats in N-trimethyl chitosan alone group darkened significantly. On POD 3, scabs and necrosis were relatively mild on the flaps of rats in rTFPI alone group and N-trimethyl chitosan-rTFPI group as compared with those of the other two groups. On POD 7, the necrosis boundaries of the flaps of rats in each group were clear. On POD 7, the flap survival rates of rats in rTFPI alone group and N-trimethyl chitosan-rTFPI group were (63±7)% and (73±5)%, respectively, which were significantly higher than (41±3)% in PBS group and (52±7)% in N-trimethyl chitosan alone group. Moreover, the flap survival rate of rats in N-trimethyl chitosan-rTFPI group was significantly higher than that in rTFPI alone group (P<0.05). On POD 7, the flaps of rats in each group had blood perfusion; the blood perfusion values in the proximal segment tissue of the rat flaps in N-trimethyl chitosan alone group and the blood perfusion values in the proximal, middle, and distal segment tissue of the rat flaps in rTFPI alone group and N-trimethyl chitosan-rTFPI group were significantly higher than those in PBS group (P<0.05 or P<0.01); the blood perfusion values in the distal segment tissue of the rat flaps in rTFPI alone group and the blood perfusion values in the middle and distal segment tissue of the rat flaps in N-trimethyl chitosan-rTFPI group were significantly higher than those in N-trimethyl chitosan alone group (P<0.05 or P<0.01); the blood perfusion value in the middle segment tissue of the rat flaps in N-trimethyl chitosan-rTFPI group was significantly higher than that in rTFPI alone group (P<0.01). On POD 7, inflammatory cells infiltrated more and cell edema was obvious in the middle segment tissue of the rat flaps in PBS group and N-trimethyl chitosan alone group. Compared with those of the previous two groups, the inflammation degree in the middle segment tissue of the rat flaps in rTFPI alone group and N-trimethyl chitosan-rTFPI group was significantly milder, the number of embolized blood vessels was significantly decreased (P<0.05 or P<0.01), and the number of new blood vessels was significantly increased (P<0.05 or P<0.01). Compared with that of rTFPI alone group, the number of new blood vessels in the middle segment tissue of the rat flaps in N-trimethyl chitosan-rTFPI group increased significantly (P<0.05). Conclusions: Loading rTFPI with N-trimethyl chitosan can achieve the effect of sustained release of rTFPI. Compared with rTFPI alone, the N-trimethyl chitosan-rTFPI complex can further improve the blood perfusion of the avulsion flaps with roll compaction in rat and improve the survival rate of the flap.

PMID:34839594 | DOI:10.3760/cma.j.cn501120-20200914-00409

Orthod Craniofac Res. 2021 Nov 28. doi: 10.1111/ocr.12551. Online ahead of print.

ABSTRACT

OBJECTIVES: To identify the 10 most numerically prolific authors publishing in the field of orthodontics for each year over the last decade (2011-2020), describe the characteristics of these outputs and identify trends in the types of study being published.

MATERIALS AND METHODS: A Scopus literature search was conducted to identify the 10 most numerically prolific publishing authors in orthodontics for each year during this decade. Number and characteristics of all publications for each author were analysed with descriptive and inferential statistics.

RESULTS: Forty-nine different individual authors were identified who were collectively prolific for between 1-8 years within the assessment decade. These authors published a total of 2025 papers, with a median annual output of 18 papers per year; however, half of these authors published between 15-24 papers per year (range 5-200). Amongst authors, 2 or more collaborated on only 7% of the identified papers. The median number of authors per paper was 5 (range 1-27) with significant variation according to study design (P<0.001). The majority of authors originated from Brazil (19.3%), Italy (14.1%) and India (12.7%). Most papers described non-prospective clinical studies (38.1%), case reports or case series (11.1%) and narrative reviews (10.8%). Finally, prolific authors had a smaller annual output when publishing in orthodontic journals (P<0.001) and when publishing experimental primary research (P=0.04).

CONCLUSION: A cohort of prolific authors in orthodontics between 2011-2020 was identified. Extreme variation was found in annual output between these authors but case reports, non-prospective clinical studies and narrative reviews predominated in their outputs.

PMID:34839575 | DOI:10.1111/ocr.12551

J Card Surg. 2021 Nov 28. doi: 10.1111/jocs.16163. Online ahead of print.

ABSTRACT

OBJECTIVE: To evaluate the effect of inhaled nitric oxide (iNO) combined with high-frequency oscillatory ventilation (HFOV) in the treatment of infants with acute hypoxemic respiratory failure (AHRF) and pulmonary hypertension (PH) after congenital heart surgery.

METHODS: A retrospective study was conducted on 63 infants with AHRF and PH after congenital heart surgery in our cardiac intensive care unit (CICU) from January 2020 to March 2021. A total of 24 infants in the A group were treated with HFOV combined with iNO, and 39 infants in the B group were treated with HFOV. Relevant clinical data were collected.

RESULTS: Comparing the two groups, the improvement of the oxygenation index, PaO₂ and PaO₂ /FiO₂ was more obvious for patients in the A group than for those in the B group after intervention (p < .05). Reexamination on the third day after the initiation of HFOV treatment indicated that the systolic pulmonary artery pressure in the A group was significantly lower than that in the B group (p < .05). In addition, the duration of mechanical ventilation and the length of CICU stay in the A group were shorter than those in the B group (p < .05). However, complications between the two groups were not statistically significant. No important adverse effects arose.

CONCLUSIONS: For infants with AHRF and PH after congenital heart surgery, iNO combined with HFOV is superior to HFOV alone to improve oxygenation, decrease pulmonary pressure, and shorten the duration of mechanical ventilation and the length of CICU stay, with no adverse effects.

PMID:34839572 | DOI:10.1111/jocs.16163

Exp Dermatol. 2021 Nov 27. doi: 10.1111/exd.14507. Online ahead of print.

ABSTRACT

This prospective, 4-week, placebo-controlled, cross-over study aimed to investigate the efficacy of 1% topical κ-opioid agonist, asimadoline, in a model of canine atopic dermatitis (AD). Fourteen beagles were challenged with house dust mites every 3-4 days for a total of 9 challenges. Severity of dermatitis was assessed and pruritus was monitored using GoPro HERO cameras. Pruritus scoring was evaluated at 10 time periods; baseline, 4 hours post allergen challenge and the last day of the study on Day 28. Scoring was done blindly by personnel using BORIS software. A global subjective score was also given using a visual analogue scale (VAS). A 4-week washout period occurred and dogs were crossed-over, the study was repeated and the results were analyzed using combined data. Gel was applied once daily on inguinal area (0.6 ml/dog). ANOVA showed significant effect of time (p<0.0001) and group (p=0.0001) on dermatitis scores. Overall, no statistically significant effect on pruritus was found due to a crossing of scores on day 17. Overtime the placebo scores increased while the active ingredient showed decrease after first 3 weeks. It is concluded that this approach is promising in dogs with AD and longer studies with more frequent application may be beneficial.

PMID:34839557 | DOI:10.1111/exd.14507

Allergy. 2021 Nov 28. doi: 10.1111/all.15192. Online ahead of print.

ABSTRACT

BACKGROUND: Asthma is a complex, multifactorial disease often linked with sensitization to house-dust mites (HDM). There is a subset of patients that does not respond to available treatments, who present a higher number of exacerbations and a worse life quality. To understand the mechanisms of poor asthma control and disease severity, we aim to elucidate the metabolic and immunologic routes underlying this specific phenotype and the associated clinical features.

METHODS: Eighty-seven patients with a clinical history of asthma were recruited and stratified in 4 groups according to their response to treatment: corticosteroid-controlled (ICS), immunotherapy-controlled (IT), biologicals-controlled (BIO) or uncontrolled (UC). Serum samples were analysed by metabolomics and proteomics; and classifiers were built using machine learning algorithms.

RESULTS: Metabolomic analysis showed that ICS and UC groups cluster separately from one another and display the highest number of significantly different metabolites among all comparisons. Metabolite identification and pathway enrichment analysis highlighted increased levels of lysophospholipids related to inflammatory pathways in the UC patients. Likewise, 8 proteins were either upregulated (CCL13, ARG1, IL15 and TNFRSF12A) or downregulated (sCD4, CCL19 and IFNγ) in UC patients compared to ICS suggesting a significant T cell activation in these patients. Finally, the machine learning model built including metabolomic and clinical data was able to classify the patients with an 87.5% accuracy.

CONCLUSIONS: UC patients display a unique fingerprint characterized by inflammatory-related metabolites and proteins, suggesting a pro-inflammatory environment. Moreover, the integration of clinical and biological data led to a deeper understanding of the mechanisms underlying UC phenotype.

PMID:34839541 | DOI:10.1111/all.15192

Perspect Psychiatr Care. 2021 Nov 28. doi: 10.1111/ppc.12971. Online ahead of print.

ABSTRACT

PURPOSE: This study was conducted to determine the relationship between health anxiety (HA) and healthy lifestyle behaviors (HLBs), and the factors that affect them.

DESIGN AND METHODS: This descriptive, cross-sectional, and relational study was conducted with 1007 voluntary participants between June 1 and September 30, 2020.

FINDINGS: The participants’ mean age was 33.46 ± 15.42 years. A positive-significant relationship was found between the participants’ total HA and HLBs scores (r = 0.06, p = 0.03). The simple linear regression analysis performed by the researchers indicated that the explanatory power of HA as a determinant of HLBs was statistically significant (R² = 0.05).

PRACTICAL IMPLICATIONS: This study determined that health anxiety increased the tendency towards HLBs.

PMID:34839532 | DOI:10.1111/ppc.12971

Risk Anal. 2021 Nov 27. doi: 10.1111/risa.13862. Online ahead of print.

ABSTRACT

Affect can influence judgments of event riskiness and use of risk-related information. Two studies (Ns: 85 and 100) examined the insensitivity-to-probability effect-where people discount probability information when scenarios are affect-rich-applying it to evidence-informed risk communication. We additionally investigated whether this effect is moderated by format, based on predictions from the evaluability and pattern-recognition literatures, suggesting that graphical formats may attenuate insensitivity to probability. Participants completed a prior beliefs questionnaire (Study 1), and risk perception booklet (both studies) that presented identical statistical information about the relative risks associated with two scenarios-one with an affect-rich outcome, the other an affect-poorer outcome. In Study 1, this was presented graphically. In Study 2, information was presented in one of three formats: written, tabular, or graphical. Participants provided their perceptions of the risk for each scenario at a range of risk-levels. The affect-rich scenario was perceived as higher in risk, and, importantly, despite presenting identical relative risk information in both scenarios, was associated with a reduced sensitivity to probability information (both studies). These differences were predicted by participants’ prior beliefs concerning the scenario events (Study 1) and were larger for the single-item written format than graphical format (Study 2). The findings illustrate that insensitivity to probability information can occur in evidence-informed risk communications and highlight how communication format can moderate this effect. This interplay between affect and format therefore reflects an important consideration for information designers and researchers.

PMID:34839529 | DOI:10.1111/risa.13862

Alcohol Clin Exp Res. 2021 Nov 28. doi: 10.1111/acer.14704. Online ahead of print.

ABSTRACT

BACKGROUND: College students affiliated with fraternity and sorority, or “Greek” life represent a known high-risk group for alcohol consumption and related consequences, but little is known about demand for alcohol in this population. The current study examined behavioral economic demand for alcohol in a sample of Greek life-affiliated undergraduate students using the alcohol purchase task (APT) and a novel variation of the APT that included a fixed-price, nonalcoholic alternative (APT Choice).

METHODS: Participants (n = 229) completed the APT, APT Choice, Alcohol Use Disorders Identification Test (AUDIT), and Daily Drinking Questionnaire (DDQ). Group demand indices were calculated for the entire sample and then separately for participants who met or did not meet the legal drinking age (21+ or underage, respectively). Independent-sample t tests assessed whether there were any significant differences between the two age cohorts in the percent change in each behavioral economic index from the APT to APT Choice. Tests of correlation evaluated the construct validity of the demand indices from both hypothetical purchase tasks.

RESULTS: Descriptive statistics on alcohol use in this Greek-affiliated sample revealed “hazardous” drinking scores, with AUDIT-C scores exceeding the threshold of alcohol misuse. These measures were significantly correlated with demand indices from both APT conditions, and demand was inversely related to price; however, demand for alcohol was reduced when a nonalcoholic alternative was available. Both age cohorts reported a reduction in BP₁ (highest price of nonzero consumption) and an increase in α (rate of change in elasticity), but these changes were significantly greater among underage participants.

CONCLUSIONS: Although Greek life-affiliated students demonstrate high demand for alcohol, the concurrent availability of a nonalcoholic alternative reduces alcohol demand, particularly for underage students. These findings suggest that nonalcoholic options may enhance the effectiveness of increasing alcohol prices to reduce alcohol consumption among students at higher risk for alcohol use.

PMID:34839527 | DOI:10.1111/acer.14704

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2021 Dec 10;38(12):1245-1249. doi: 10.3760/cma.j.cn511374-20200913-00667.

ABSTRACT

OBJECTIVE: To explore the genetic basis for a Chinese pedigree affected with mental retardation.

METHODS: G-banded karyotyping analysis and single nucleotide polymorphism microarray (SNP array) were used to detect the genetic variants within the family, and the origin of the variants was analyzed using UPDtool Statistics software.

RESULTS: The patient, a 26-year-old female, was found to have a chromosomal karyotype of 46,XX,dup(4)(q28.2q31.3),and SNP array revealed a 25.71 Mb duplication at 4q28.2-q31.3. The duplication was inherited from her father, and her fetus was found to carry the same duplication.

CONCLUSION: The duplication of the patient probably underlay the mental retardation. The gender of the carrier and parental origin of the duplication might have led to the variation in their clinical phenotype.

PMID:34839517 | DOI:10.3760/cma.j.cn511374-20200913-00667

Hu Li Za Zhi. 2021 Dec;68(6):43-52. doi: 10.6224/JN.202112_68(6).07.

ABSTRACT

BACKGROUND: Night-shift work affects the physical and mental health of employees. Early identification of relevant high-risk groups may be referenced in health promotion, job analysis, and job design.

PURPOSE: To explore the differences in workplace fatigue, physiological index of metabolic syndrome, and risk of cardiovascular disease between long-term and non-long-term night-time working nursing staff.

METHODS: The health examination data of 91 long-term and 119 non-long-term night-shift nurses were collected. A sub-data analysis approach was used and t-test, chi-square, Pearson`s correlation, and hierarchical multiple regression were used to conduct statistical analysis.

RESULTS: A positive correlation between cardiovascular risk and workplace fatigue was found in the long-term night-shift working group. Moreover, a lower average duration of sleep was found in the non-long-term night-shift working group. Finally, the participants with workplace fatigue and metabolic syndrome were found to have higher levels of cardiovascular risk.

CONCLUSIONS / IMPLICATIONS FOR PRACTICE: Nursing staff who work night shifts for long-term and non-long-term periods should all receive regular health check-ups and practice healthy eating, exercise, and sleep habits. Employers should provide regular health checkups to their nursing staff and implement measures to identify health hazards in accordance with laws on labor standards, occupational safety, and health and labor health protection regulations, and then adopt appropriate plans and necessary safety and health measures to achieve a mutually beneficial result for both employers and employees in the healthcare industry.

PMID:34839490 | DOI:10.6224/JN.202112_68(6).07