Nevin Manimala

Front Oncol. 2022 Mar 3;12:813138. doi: 10.3389/fonc.2022.813138. eCollection 2022.

ABSTRACT

PURPOSE: This study aims to evaluate the value of 3.0T MRI Intravoxel Incoherent motion diffusion-weighted magnetic resonance imaging (IVIM-DWI) combined with texture analysis (TA) for evaluating extramural vascular invasion (EMVI) of rectal adenocarcinoma.

METHODS: Ninety-six patients with pathologically confirmed rectal adenocarcinoma after surgical resections were collected. Patients were divided into the EMVI positive group (n=39) and the EMVI negative group (n=57). We measured the IVIM-DWI parameters and TA parameters of rectal adenocarcinoma. We compare the differences of the above parameters between the two groups and establish a prediction model through multivariate logistic regression analysis. the ROC curve was performed for parameters with each individual and in combination.

RESULTS: ADC, D, D* value between the two groups were statistically significant (P= 0.015,0.031,0). Six groups of texture parameters were statistically significant between the two groups (P=0.007,0.037,0.011,0.005,0.007,0.002). Logistic regression prediction model shows that GLCM entropy_ALL DIRECTION_offset7_SD and D* are important independent predictors, and the AUC of the regression prediction model was 0.821, the sensitivity was 92.98%, the specificity was 61.54%, and the Yoden index was 0.5452. The AUC was significantly higher than that of other single parameters.

CONCLUSION: 3.0T MRI IVIM-DWI parameters combined with texture analysis can provide valuable information for EMVI evaluation of rectal adenocarcinoma before the operation.

PMID:35311135 | PMC:PMC8927647 | DOI:10.3389/fonc.2022.813138

Front Oncol. 2022 Mar 3;12:784839. doi: 10.3389/fonc.2022.784839. eCollection 2022.

ABSTRACT

PURPOSE: This study aimed to assess the diagnostic performance and the added value to radiologists of different levels of a computer-aided diagnosis (CAD) system for the detection of pathological complete response (pCR) after neoadjuvant chemotherapy (NAC) in patients with breast cancer. Besides, to investigate whether tumor molecular typing is associated with the efficiency of diagnosis of the CAD systems.

METHODS: 470 patients were identified with breast cancers who underwent NAC and post MR imaging between January 2016 and March 2019. The diagnostic performance of radiologists of different levels and the CAD system were compared. The added value of the CAD system was assessed and subgroup analyses were performed according to the tumor molecular typing.

RESULTS: Among 470 patients, 123 (26%) underwent pCR. The CAD system showed a comparable specificity as the senior radiologist (83.29% vs. 84.15%, p=0.488) and comparable area under the curve (AUC) (0.839 vs. 0.835, p =0.452). The performance of all radiologists significantly improved when aided by the CAD system (P<0.05), And there were no statistical differences in terms of sensitivity, specificity and accuracy between the two groups with CAD assistance(p>0.05).The AUC values for identifying pCR in TN patients were significant (0.883, 95%CI: 0.801-0.964, p < 0.001).

CONCLUSION: The CAD system assessed in this study improves the performance of all radiologists, regardless of experience. The molecular typing of breast cancer is potential influencer of CAD diagnostic performance.

PMID:35311124 | PMC:PMC8928462 | DOI:10.3389/fonc.2022.784839

Front Oncol. 2022 Mar 3;12:860532. doi: 10.3389/fonc.2022.860532. eCollection 2022.

ABSTRACT

Since the 20th century, cancer has been a growing threat to human health. Cancer is a malignant tumor with high clinical morbidity and mortality, and there is a high risk of recurrence after surgery. At the same time, the diagnosis of whether the cancer is in situ recurrence is crucial for further treatment of cancer patients. According to statistics, about 90% of cancer-related deaths are due to metastasis of primary tumor cells. Therefore, the study of the location of cancer recurrence and its influencing factors is of great significance for the clinical diagnosis and treatment of cancer. In this paper, we propose an assisted diagnosis model for cancer patients based on federated learning. In terms of data, the influencing factors of cancer recurrence and the special needs of data samples required by federated learning were comprehensively considered. Six first-level impact indicators were determined, and the historical case data of cancer patients were further collected. Based on the federated learning framework combined with convolutional neural network, various physical examination indicators of patients were taken as input. The recurrence time and recurrence location of patients were used as output to construct an auxiliary diagnostic model, and linear regression, support vector regression, Bayesling regression, gradient ascending tree and multilayer perceptrons neural network algorithm were used as comparison algorithms. CNN’s federated prediction model based on improved under the condition of the joint modeling and simulation on the five types of cancer data accuracy reached more than 90%, the accuracy is better than single modeling machine learning tree model and linear model and neural network, the results show that auxiliary diagnosis model based on the study of cancer patients in assisted the doctor in the diagnosis of patients, As well as effectively provide nutritional programs for patients and have application value in prolonging the life of patients, it has certain guiding significance in the field of medical cancer rehabilitation.

PMID:35311106 | PMC:PMC8928102 | DOI:10.3389/fonc.2022.860532

Front Oncol. 2022 Mar 4;12:834992. doi: 10.3389/fonc.2022.834992. eCollection 2022.

ABSTRACT

Majority of patients with resected early- and intermediate-stage liver cancer will experience postoperative recurrence. This study aimed to investigate the application of ctDNA sequencing in the postoperative period of hepatocellular carcinoma. A total of 96 patients with liver cancer were enrolled in this study. Postoperative peripheral blood samples were collected from all patients after surgery and analyzed using hybridization capture-based next-generation sequencing. Identification of at least one somatic mutation in the peripheral blood was defined as ctDNA+. Five genetic features in tumor tissues were associated with disease-free survival (DFS) using Lasso-Cox model. The area under the receiver operating characteristic curve was 0.813 and 0.882 in training and validation cohorts, respectively. The recurrence rate in ctDNA+ and ctDNA- groups was 60.9% and 27.8%, respectively. Multivariate Cox regression analysis showed that the postoperative ctDNA was an independent prognostic predictor of DFS (HR [hazard ratio]: 6.074, 95% Cl [confidence interval]: 2.648-13.929, P<0.001) and overall survival (OS) (HR: 4.829, 95% CI: 1.508-15.466, P=0.008). Combined ctDNA with AFP improved prediction performance. The median DFS was 2.0, and 8.0 months in ctDNA+/AFP-H and ctDNA+/AFP-L groups, respectively; while ctDNA-/AFP-H and ctDNA-/AFP-L groups had not reached the median time statistically (Log-rank test, P < 0.0001). Furthermore, ctDNA- patients had better prognosis than ctDNA+ patients irrespective of tumor stage. Postoperative ctDNA sequencing has great prognostic value in patients with liver cancer. Patients with positive ctDNA should receive more intensive disease monitoring and more aggressive treatment strategies to improve the survival time.

PMID:35311090 | PMC:PMC8931326 | DOI:10.3389/fonc.2022.834992

Front Pediatr. 2022 Mar 4;10:778378. doi: 10.3389/fped.2022.778378. eCollection 2022.

ABSTRACT

OBJECTIVES: The heterogeneity of sepsis makes it difficult to predict outcomes using existing severity of illness tools. The vasoactive-inotrope score (VIS) is a quantitative measure of the amount of vasoactive support required by patients. We sought to determine if a higher aggregate VIS over the first 96 h of vasoactive medication initiation is associated with increased resource utilization and worsened clinical outcomes in pediatric patients with severe sepsis.

DESIGN: Retrospective cohort study.

SETTING: Single-center at Children’s Wisconsin in Milwaukee, WI.

PATIENTS: One hundred ninety-nine pediatric patients, age less than 18 years old, diagnosed with severe sepsis, receiving vasoactive medications between January 2017 and July 2019.

INTERVENTIONS: Retrospective data obtained from the electronic medical record, calculating VIS at 2 h intervals from 0-12 h and at 4 h intervals from 12-96 h from Time 0.

MEASUREMENTS: Aggregate VIS derived from the hourly VIS area under the curve (AUC) calculation based on the trapezoidal rule. Data were analyzed using Pearson’s correlations, Mann-Whitney test, Wilcoxon signed rank test, and classification, and regression tree (CART) analyses.

MAIN RESULTS: Higher aggregate VIS is associated with longer hospital LOS (p < 0.0001), PICU LOS (p < 0.0001), MV days (p = 0.018), increased in-hospital mortality (p < 0.0001), in-hospital cardiac arrest (p = 0.006), need for ECMO (p < 0.0001), and need for CRRT (p < 0.0001). CART analyses found that aggregate VIS >20 is an independent predictor for in-hospital mortality (p < 0.0001) and aggregate VIS >16 for ECMO use (p < 0.0001).

CONCLUSIONS: There is a statistically significant association between aggregate VIS and many clinical outcomes, allowing clinicians to utilize aggregate VIS as a physiologic indicator to more accurately predict disease severity/trajectory in pediatric sepsis.

PMID:35311061 | PMC:PMC8931266 | DOI:10.3389/fped.2022.778378

Front Pediatr. 2022 Mar 4;10:834825. doi: 10.3389/fped.2022.834825. eCollection 2022.

ABSTRACT

Despite the importance of effective communication skills in pediatrics, clinical placements may inadequately prepare undergraduate students to communicate with children. The integration of non-clinical interactions with healthy children within a pediatric curriculum has the potential to enhance learning. We designed and implemented a novel course involving experiential learning, including video-recorded consultations with simulated parents (SPs), team-based scenarios with a pediatric mannequin, interactions with healthy children through a pre-school visit and medical student led health workshops for primary school children. Medical students at the RCSI University of Medicine and Health Sciences took part in the course. We used a mixed methods approach to assess the impact of the course. We investigated medical students’ perspectives through a pre- and post-intervention questionnaire and post-intervention focus group discussions (FGDs). We assessed participating children’s health literacy at the start of the course. 144/279 (51.6%) of the fourth year medical student cohort on their pediatric rotation, consented to participate in the study. All 144 (100%) of consenting students completed the pre-intervention questionnaire. 59/144 (40.1%) of consenting students completed the post-intervention questionnaire. Results showed a statistically significant improvement in ratings (p < 0.05) for items related to managing a confrontational situation involving family members, completing a psychosocial assessment with an adolescent and effectiveness using evidence-based medicine (EBM) when motivating patients. There was a statistically significant decrease in how students rated their comfort at using EBM when motivating patients. Four themes relating to how students experienced the intervention were identified from eight FGDs (n = 35 students): Shaping Student Learning; Supporting Student Learning; Developing New Skills and Feeling More Prepared. 39/49 (79.6%) children completed a health literacy assessment. All questions had a high percentage of positive responses. Question 7, understanding your doctor, had the highest proportion of negative responses (27%). Ours is one of the first studies to design an educational intervention to enhance pediatrics teaching by combining interactions with healthy children outside of a clinical setting with more traditional simulation-based approaches. We conclude that this type of intervention supports students’ learning of pediatric communication skills and enhances students’ perceived preparation for clinical placement.

PMID:35311060 | PMC:PMC8931532 | DOI:10.3389/fped.2022.834825

Front Pediatr. 2022 Mar 2;10:813528. doi: 10.3389/fped.2022.813528. eCollection 2022.

ABSTRACT

BACKGROUND: Extracorporeal membrane oxygenation (ECMO) is increasingly being used to support patients after the repair of congenital heart disease.

OBJECTIVE: We report our experience with patients with a single functional ventricle who were supported by ECMO after the Norwood procedure, reviewing the outcomes and identifying risk factors for mortality in these patients.

METHODS: In this single-center retrospective cohort study, we enrolled 33 patients with hypoplastic left heart syndrome (HLHS) who received ECMO support after the Norwood procedure between January 2015 and December 2019. The independent variables evaluated in this study were demographic, anatomical, and those directly related to ECMO support (ECMO indication, local of initiation, time under support, and urinary output while on ECMO). The dependent variable was survival. A p < 0.05 was considered statistically significant.

RESULTS: The ECMO support was applied in 33 patients in a group of 120 patients submitted to Norwood procedure (28%). Aortic atresia was present in 72.7% of patients and mitral atresia in 51.5%. For 15% of patients, ECMO was initiated in the operating room; for all other patients, ECMO was initiated in the intensive care unit. The indications for ECMO in the cardiac intensive care unit were cardiac arrest in 22 (79%) of patients, low cardiac output state in 10 (18%), and arrhythmia in 1 patient (3%). The median time under support was 5 (2-25) days. The median follow-up time was 59 (4-150) days. Global survival to Norwood procedure was 90.9% during the 30-day follow-up, being 33.3% for those submitted to ECMO. Longer ECMO support (p = 0.004) was associated with a higher risk of death in the group submitted to ECMO.

CONCLUSIONS: The mortality of patients with HLHS who received ECMO support after stage 1 palliation was high. Patients with low urine output were related to worse survival rates, and longer periods under ECMO support (more than 9 days of ECMO) were associated with 100% mortality. Earlier ECMO initiation before multiorgan damage may improve results.

PMID:35311057 | PMC:PMC8926323 | DOI:10.3389/fped.2022.813528

Front Pediatr. 2022 Mar 3;10:795019. doi: 10.3389/fped.2022.795019. eCollection 2022.

ABSTRACT

BACKGROUND: The efficacy and safety of impregnated central venous catheters (CVCs) in pediatrics remain controversial. The purpose of this study was to evaluate the efficacy of impregnations for the prevention of catheter-related bloodstream infection (CRBSI).

METHODS: We searched the following five electronic databases: Medline, PubMed, Cochrane, Embase, and the Web of Science for randomized controlled trials (RCTs) up to March 2021. Pooled risk ratios (RRs) with 95% confidence intervals (CIs) were calculated using a fixed-effects model. Assessment of publication biases was evaluated by Egger’s test. Heterogeneity between studies was assessed based on the chi-square test and I ² statistics, and sensitivity analysis and subgroup analysis were also performed.

RESULTS: A total of six RCTs with 3,091 patients were included. Impregnated CVCs provided significant benefits in reducing the risk of CRBSI (RR = 0.41, 95% CI: 0.26-0.66) in pediatric patients, especially in the pediatric group. No publication bias was observed in the Egger test for the risk of CRBSI. Drug type is a source of heterogeneity.

CONCLUSION: Antimicrobial-impregnated CVCs are beneficial to prevent CVC-related complications in pediatrics.

PMID:35311042 | PMC:PMC8927082 | DOI:10.3389/fped.2022.795019

Int J Endocrinol. 2022 Mar 10;2022:7847011. doi: 10.1155/2022/7847011. eCollection 2022.

ABSTRACT

Chronic skin wound caused by diabetic disease is very common worldwide. Moreover, there is a shortage of effective curing technology in clinic. In this work, we developed a novel technology using thermosensitive hydrogel on wound top combined with insulin injection. The efficiency and mechanism of this technology were investigated in a diabetic mouse model. Dorsal-paired 8-10 mm diameter wounds were created in 12 mice. The wound healing rate was determined over a 28-day interval in healthy control (Control), control with diabetes (DControl), poloxamer treatment (Pox), and poloxamer plus insulin injection (Poxin) mice. Histological specimens were observed in all samples. Real-time quantitative polymerase chain reaction (qRT-PCR) was performed to measure the relative expression of α-smooth muscle actin (α-SMA) and transforming growth factor beta 1 (TGF-β1) in wound tissues at 7, 14, and 28 days. Compared with DControl animals, those treated with Poxin showed accelerated wound closure and healing rate (p < 0.05); expression of both α-SMA and TGF-β1 was significantly higher than that of the DControl and Pox animals during the first 7 days postoperation, but a significant decrease at day 14. Therefore, we concluded that hydrogel combined with insulin accelerated wound healing. Controlling the glucose level via insulin injection is more beneficial than hydrogel alone for healing chronic wounds, potentially through the increase of α-SMA and TGF-β1 expression in early phase.

PMID:35311032 | PMC:PMC8930262 | DOI:10.1155/2022/7847011

Int J Endocrinol. 2022 Mar 10;2022:3830380. doi: 10.1155/2022/3830380. eCollection 2022.

ABSTRACT

INTRODUCTION: The interactions of central obesity and body composition with thyroid hormones and the hypothalamus-pituitary-adrenal (HPA) axis are unclear; both central obesity and body composition have an impact on energy homeostasis. Our study aimed to investigate the association between body composition and pituitary hormones, including the HPA axis and pituitary-thyroid axis, in a Chinese population of euthyroid overweight and obese individuals.

METHODS: This was a cross-sectional study. Overweight and obese patients who regularly visited the multidisciplinary team (MDT) for obesity at Peking University First Hospital were enrolled in the study. Thyroid function, morning serum ACTH and cortisol levels, thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TgAb), body composition, and metabolic indicators, including liver function and the lipid profile, were measured at the first visit. Statistical analysis was performed using SPSS version 21.0 (IBM, USA).

RESULTS: In total, 441 patients with overweight or obesity were enrolled (male/female, 123/318). Patients were assigned to four groups according to the thyroid-stimulating hormone (TSH) level stratified by quartiles, and increased body mass index (BMI) was revealed in the highest TSH quartile group (p=0.002). Hip circumference (HC) of patients in the highest TSH quartile group was significantly increased (p=0.021). Morning ACTH levels and fasting insulin levels were significantly elevated in patients in the highest TSH quartile group (p=0.027 for fasting insulin, p < 0.001 for ACTH). In the female subgroup, patients in the highest TSH quartile group showed increases in BMI (p=0.010), waist circumference (WC) (p=0.007), muscle mass of the lower extremities (p=0.020), fasting C-peptide (p=0.031), and ACTH (p=0.002). In the male subgroup, patients in the highest TSH quartile group exhibited higher BMI (p=0.017), HC (p=0.036), and ACTH (p=0.003). Among patients in the highest ACTH quartile group, there was an elevated proportion of males (p=0.003), and FT3 (p=0.005), fasting insulin (p=0.037), and cortisol (p < 0.001) levels were increased. Weight (p < 0.001), BMI (p < 0.001), WC (p < 0.001), HC (p < 0.001), muscle mass of the upper extremities (p=0.003), muscle mass of the lower extremities (p=0.005), and total muscle mass (p=0.003) were elevated in patients in the highest ACTH quartile group. HC was found to be an independent factor after adjustment for other confounders and was positively associated with the TSH level (p=0.004 for the regression model, B = 0.152, p=0.004).

CONCLUSIONS: BMI is positively correlated with TSH and ACTH levels in both male and female obese individuals. The ACTH level was positively associated with male sex and increased BMI and muscle mass. Hip circumference was an independent factor that was positively related to TSH levels.

PMID:35311031 | PMC:PMC8930244 | DOI:10.1155/2022/3830380