Nevin Manimala

Toxins (Basel). 2022 Jul 12;14(7):476. doi: 10.3390/toxins14070476.

ABSTRACT

This study investigated the occurrence of 5 unregulated mycotoxins in a total of 250 traditional dry-cured meat products sampled in 2020 and 2021 in five Croatian regions (eastern, northern, central, western, and southern). Aflatoxin B₁ (AFB₁), ochratoxin A (OTA), sterigmatocystin (STC), citrinin (CIT), and cyclopiazonic acid (CPA) concentrations were related to the geographical region of the product’s origin and to local weather. The results revealed the contamination of 27% of samples, namely, STC in 4% of samples in concentrations of up to 3.93 µg/kg, OTA in 10% of samples in concentrations of up to 4.81 µg/kg, and CPA in 13% of samples in concentrations of up to 335.5 µg/kg. No AFB₁ or CIT contamination was seen. Although no statistically significant differences in concentrations of individual mycotoxins across the production regions were found, differences in mycotoxin occurrence were revealed. The eastern and western regions, with moderate climate, delivered the largest number of contaminated samples, while the southern region, often compared with subtropics, delivered the smallest, so that the determined mycotoxins were probably mainly produced by the Penicillium rather than the Aspergillus species. Due to the interaction of various factors that may affect mycotoxin biosynthesis during production, the detected concentrations cannot be related solely to the weather.

PMID:35878214 | DOI:10.3390/toxins14070476

Toxins (Basel). 2022 Jun 29;14(7):441. doi: 10.3390/toxins14070441.

ABSTRACT

This study assessed the effects of botulinum toxin type A (BoNT-A) in mandibular range of motion and muscle tenderness to palpation in persistent myofascial pain (MFP) patients (ReBEC RBR-2d4vvv). Eighty consecutive female subjects with persistent MFP, were randomly divided into four groups (n = 20): three BoNT-A groups with different doses and a saline solution group (placebo control group). Treatments were injected bilaterally in the masseter and anterior temporalis muscle in a single session. Clinical measurements of mandibular movements included: pain-free opening, maximum unassisted and assisted opening, and right and left lateral excursions. Palpation tests were performed bilaterally in the masseter and temporalis muscle. Follow-up occurred 28 and 180 days after treatment. For the statistical analysis the Mann-Whitney U-test with Bonferroni correction was used for groups comparisons. Regardless of dose, all parameters of mandibular range of motion significantly improved after 180 days in all BoNT-A groups, compared with the control group. Palpation pain over the masseter and temporalis muscles were significantly reduced in all BoNT-A groups regardless of dose, compared with the control group, after 28 and 180 days of treatment. Independent of doses, BoNT-A improved mandibular range of motion and muscle tenderness to palpation in persistent MFP patients.

PMID:35878179 | DOI:10.3390/toxins14070441

Toxins (Basel). 2022 Jun 24;14(7):430. doi: 10.3390/toxins14070430.

ABSTRACT

Due to the climatic change, an increase in aflatoxin B1 (AFB1) maize contamination has been reported in Europe. As an alternative to mineral binders, natural phytogenic compounds are increasingly used to counteract the negative effects of AFB1 in farm animals. In cows, even low dietary AFB1 concentrations may result in the milk excretion of the genotoxic carcinogen metabolite aflatoxin M1 (AFM1). In this study, we tested the ability of dietary turmeric powder (TP), an extract from Curcuma longa (CL) rich in curcumin and curcuminoids, in reducing AFM1 mammary excretion in Holstein-Friesian cows. Both active principles are reported to inhibit AFM1 hepatic synthesis and interact with drug transporters involved in AFB1 absorption and excretion. A crossover design was applied to two groups of cows (n = 4 each) with a 4-day washout. Animals received a diet contaminated with low AFB1 levels (5 ± 1 µg/kg) for 10 days ± TP supplementation (20 g/head/day). TP treatment had no impact on milk yield, milk composition or somatic cell count. Despite a tendency toward a lower average AFM1 milk content in the last four days of the treatment (below EU limits), no statistically significant differences with the AFB1 group occurred. Since the bioavailability of TP active principles may be a major issue, further investigations with different CL preparations are warranted.

PMID:35878168 | DOI:10.3390/toxins14070430

Trop Med Infect Dis. 2022 Jul 7;7(7):126. doi: 10.3390/tropicalmed7070126.

ABSTRACT

Fascioliasis is a parasitic infection that affects both livestock and humans. Understanding the distribution of Fasciola spp. can help the development of preventive measures to control fascioliasis. This systematic review and meta-analysis aimed to estimate the status of fascioliasis among livestock and humans in Pakistan between 2000 and 2020. Based on the selection criteria, 25 articles were selected from Google Scholar, PubMed, and Scopus. This review included 76,099 animals, including 13,738 that were positive for fascioliasis. The overall prevalence was 18.1%; it was 0.3% in humans and 20.1% in livestock. Among animal hosts, the prevalence was highest in sheep (53.5%), followed by the goats (44.9%), cows (21.3%), buffaloes (16.8%), cattle (12.7%), and humans (0.3%). Sindh had the highest prevalence at 42.7%, followed by Baluchistan (25.2%), Punjab (17.7%), Khyber Pakhtunkhwa (10.7%), and Islamabad capital territory (1.5%). In the Punjab province, sheep had the highest prevalence (65.7%); in Khyber Pakhtunkhwa, it was buffalo (15.9%); and in Baluchistan, it was cows (28.5%). The prevalence of Fasciola spp. was higher in Sindh and Baluchistan than in the other provinces. The presented results are essential for developing preventive approaches for the management of human health and minimizing economic loss in the livestock industry in Pakistan. Preventive-curative treatments two times a year followed by a prophylactic treatment at the end of the dry season are crucial throughout the areas of Pakistan that serve as hotspots for infection by Fasciola sp. For humans, regular, prioritized surveys must be performed for high-risk populations so that the real situation can be assessed and addressed in a timely manner.

PMID:35878138 | DOI:10.3390/tropicalmed7070126

Trop Med Infect Dis. 2022 Jul 1;7(7):124. doi: 10.3390/tropicalmed7070124.

ABSTRACT

BACKGROUND: Zoonotic diseases account for more than 70% of emerging infectious diseases (EIDs). Due to their increasing incidence and impact on global health and the economy, the emergence of zoonoses is a major public health challenge. Here, we use a biogeographic approach to predict future hotspots and determine the factors influencing disease emergence. We have focused on the following three viral disease groups of concern: Filoviridae, Coronaviridae, and Henipaviruses.

METHODS: We modelled presence-absence data in spatially explicit binomial and zero-inflation binomial logistic regressions with and without autoregression. Presence data were extracted from published studies for the three EID groups. Various environmental and demographical rasters were used to explain the distribution of the EIDs. True Skill Statistic and deviance parameters were used to compare the accuracy of the different models.

RESULTS: For each group of viruses, we were able to identify and map areas at high risk of disease emergence based on the spatial distribution of the disease reservoirs and hosts of the three viral groups. Common influencing factors of disease emergence were climatic covariates (minimum temperature and rainfall) and human-induced land modifications.

CONCLUSIONS: Using topographical, climatic, and previous disease outbreak reports, we can identify and predict future high-risk areas for disease emergence and their specific underlying human and environmental drivers. We suggest that such a predictive approach to EIDs should be carefully considered in the development of active surveillance systems for pathogen emergence and epidemics at local and global scales.

PMID:35878136 | DOI:10.3390/tropicalmed7070124

Trop Med Infect Dis. 2022 Jun 23;7(7):115. doi: 10.3390/tropicalmed7070115.

ABSTRACT

(1) Background: Olfactory and gustatory dysfunctions (OGDs) was found in COVID-19 patients. Only a few studies looked into the prevalence of OGDs in the United Arab Emirates (UAE). The purpose of this study was to estimate the prevalence OGDs among multiethnic COVID-19 patients in the UAE, and its association to patients’ characteristics and disease outcomes; (2) Methods: There were 1785 COVID-19 patients included in our cohort; (3) Results: Males made up most of the study participants (86.3%). A total of 11.7% of the participants reported OGDs. Female gender and ethnicity had significantly higher symptom prevalence (p < 0.001). COVID-19 severity had a strong inverse association with OGDs (p = 0.007). Other illness outcomes, such as ICU admission, pneumonia development, and mortality, showed no correlation. Males, Asians, and patients with comorbidities all had statistically significantly lower prevalence odds. On the other hand, Emirati, Arab, and Iranian patients had a higher prevalence. COVID-19 patients with OGDs had a considerably shorter time until viral clearance than those without the symptom; (4) Conclusions: in nonsevere COVID-19, olfactory/gustatory dysfunction is common. As a result, it could be applied as a predictive sign for early disease diagnosis and prognosis.

PMID:35878127 | DOI:10.3390/tropicalmed7070115

Trop Med Infect Dis. 2022 Jun 21;7(7):113. doi: 10.3390/tropicalmed7070113.

ABSTRACT

The elimination of lymphatic filariasis (LF) is achieved through repeated mass drug administration (MDA) of anti-filarial medications, which interrupts transmission and prevents new infections. Accurate transmission assessments are critical to deciding when to stop MDA. Current methods for evaluating transmission may be insufficiently sensitive, resulting in post-MDA resurgence. We, therefore, evaluated potential diagnostic testing scenarios for post-MDA surveillance. Data were used from two surveys (a household cluster and a cohort) conducted in an area of Mandalay Region, Myanmar, with ongoing transmission following several rounds of MDA. First, age- and sex-adjusted seroprevalence were estimated for the area using the household survey. Next, three Bayesian networks were built from the combined datasets to compare antigens by immunochromatic testing (ICT) and/or Og4C3 enzyme-linked immunosorbent assay (ELISA) and antibody (Ab) detection methods (Wb123 or Bm14 Ab ELISA). The networks were checked for validity and then used to compare diagnostic testing scenarios. The adjusted prevalence from the household survey for antigen, Wb123 Ab and Bm14 Ab were 4.4% (95% CI 2.6-7.3%), 8.7% (5.96-12.5%) and 20.8% (16.0-26.6%), respectively. For the three networks, the True Skill Statistic and Area Under the Receiver Operating Characteristic Curve for antigen, Wb123 and Bm14 Ab were 0.79, 0.68 and 0.55; and 0.97, 0.92 and 0.80, respectively. In the Bayesian network analysis, a positive case was defined as testing positive to one or more infection markers. A missed result was therefore the probability of a positive case having a negative test result to an alternate marker. The probability of a positive case prior to any testing scenario was 17.4%, 16.8% and 26.6% for antigen, Wb123 Ab and Bm14 Ab, respectively. In the antigen-only testing scenario, the probability of a missed positive LF result was 5.2% for Wb123 and 15.6% for Bm14 Ab. The combination of antigen plus Bm14 Ab testing reduced the probability of missing a positive LF case as measured by Wb123 Ab to 0.88%. The combination of antigen plus Wb123 Ab was less successful and yielded an 11.5% probability of a missed positive result by Bm14 Ab testing. Across scenarios, there was a greater discordance between Bm14 and both antigen and Wb123 Ab in the 1-10 age group compared to older ages. These findings suggest that the addition of Bm14 Ab improves the sensitivity of LF testing for current or past infection. The combination of antigen plus Bm14 Ab should therefore be considered for inclusion in post-MDA surveillance to improve the sensitivity of transmission surveys and prevent the premature cessation of MDA.

PMID:35878125 | DOI:10.3390/tropicalmed7070113

Sports (Basel). 2022 Jul 6;10(7):108. doi: 10.3390/sports10070108.

ABSTRACT

The aim of this cross-sectional study was to determine the differences in the morphological characteristics and situational precision among younger and older groups of handball players. The sample of participants consisted of 30 handball players, members of the younger category of the Al-Ahli handball club (Bahrein), divided into two groups: older (U16, n = 18) and younger (U15, n = 12). To evaluate their morphological characteristics, eight variables were measured, while two standardized tests were used to evaluate their situational precision. The results indicate that a statistically significant difference between the groups was noticeable for nine variables in total, seven in morphology (body height, p = 0.010; body mass index, p = 0.049; arm length, p = 0.009; upper arm length, p = 0.016; lower arm length, p = 0.040; the planimetric parameter of the hand, p = 0.005; hand length p = 0.004) and two in situational precision (the standing shot, p = 0.003; the jump shot, p = 0.17), and that the achieved difference ranges from a medium to a large effect. For only one variable (body mass, p = 0.734), significant difference was not determined between the groups. It was also determined (by Cohen’s criterion) that handball players with higher longitudinal dimensionality achieve better results for specific precision. Therefore, when selecting young handball players, the aforementioned dimensions should be taken into consideration as predictors of success.

PMID:35878119 | DOI:10.3390/sports10070108

Sports (Basel). 2022 Jun 29;10(7):103. doi: 10.3390/sports10070103.

ABSTRACT

Organizational initiatives and researchers have argued for the importance of the natural outdoor environment (NOE) for promoting wellbeing. The main aim of this meta-analysis was to synthesize the existing literature to examine the effects of physical activity (PA) in the NOE on wellbeing in adults. The secondary aim was to explore whether wellbeing reported by adults differs as a function of PA context. Electronic databases (PubMed, ProQuest Nursing and Allied Health, PsycINFO, SPORTDiscus and Embase) were searched for English peer-reviewed articles published before January 2019. Inclusion criteria were: (1) healthy adults; (2) PA in the NOE; (3) the measurement of wellbeing; and (4) randomized control trials, quasi-experimental designs, matched group designs. To address the secondary aim, PA in the NOE was compared with that performed indoors. Risk of bias was assessed through the Effective Public Health Practice Project (EHPP) Quality Assessment Tool for Quantitative Studies. Primary studies meeting inclusion criteria for the main (n_studies = 19) and secondary (n_studies = 5) aims were analyzed and interpreted. The overall effect size for the main analysis was moderate (d = 0.49, p < 0.001; 95% CI = 0.33, 0.66), with the magnitude of effect varying depending on wellbeing dimension. Wellbeing was greater in PA in the NOE subgroup (d = 0.53) when compared with the indoor subgroup (d = 0.28), albeit not statistically significant (p = 0.15). Although physical activity in the NOE was associated with higher wellbeing, there is limited evidence to support that it confers superior benefits to that engaged indoors. Researchers are encouraged to include study designs that measure markers of wellbeing at multiple time points, greater consideration to diverse wellbeing dimensions and justify decisions linked to PA and NOE types.

PMID:35878114 | DOI:10.3390/sports10070103

Sports (Basel). 2022 Jun 28;10(7):101. doi: 10.3390/sports10070101.

ABSTRACT

(1) The purpose of this study was to examine the prevalence of the relative age effect (RAE) in the best young (10 to 15 years old) track and field athletes. (2) Hypothesis: The prevalence of the RAE in the best young track and field athletes of both genders will be evident in all age groups from 10 to 15 years old, which may be associated with the significant relationship between biological maturity, chronological age, and the development of physical qualities. (3) Materials and methods: In total, 1778 athletes volunteered for this study. The sample was based on the results of the best young athletes who participated in the final tournaments of the national competition “Shipovka Yunykh”(“Running spikes for young athletes”), which have been held since 1981. The sample group consisted of male and female athletes classified into specific age groups: 10 to 11 years old (n = 579), 12 to 13 years old (n = 600), and 14 to 15 years old (n = 599). Analysis was performed using Jamovi 1.8.1. The Chi-square test was used to compare the RAE between different groups. (4) Results: A wide distribution of the RAE was revealed both in the general sample and in boys and girls. The percentage of “early-born” athletes was 37.6% while only 12.3% were “late-born” athletes. The difference in the severity of the RAE may reflect the small sample of athletes from the fourth quartile, which was significantly less than the sample of boys from the fourth quartile (p = 0.04, OR 1.68, 95% CI 1.02-2.78). The RAE was also evident in all age groups of boys and girls, without any statistically significant differences in the severity (p > 0.05, Chi = 2.135, V = 0.02). In the 14- to 15-year-old male athletes group, the number of early-born compared to late-born athletes peaked. The RAE was most common amongst the most successful track and field athletes. Among the competition medalists during the analyzed time period, more than 50% of athletes were born in the first quarter and no athletes were born in the fourth quarter.

PMID:35878112 | DOI:10.3390/sports10070101