Nevin Manimala

Am J Trop Med Hyg. 2021 Oct 4:tpmd210584. doi: 10.4269/ajtmh.21-0584. Online ahead of print.

ABSTRACT

Dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) are arboviruses that can affect maternal and fetal outcome if acquired during pregnancy. This study was done to estimate the positivity of DENV, CHIKV, and ZIKV in febrile pregnant women attending a tertiary care hospital in north India. Symptomatic pregnant women were tested for these viruses by IgM ELISA and/or by Trioplex real-time polymerase chain reaction. Their symptoms and laboratory parameters were recorded and were followed up till delivery to know their immediate delivery outcome. Of 104 women tested, 50 (48.1%) were positive for viral markers. Of these, evidence of infection by DENV, CHIKV, and both was found in 34 (32.7%), 10 (9.6%), and 6 (5.8%), respectively. ZIKV was not detected in any woman. Maximum DENV positivity occurred in the third trimester of pregnancy and in women residing in urban than rural areas. Chills and rigors, arthralgia, retro-orbital pain, anemia, and vaginal bleeding were more commonly associated with DENV positivity. Backache, arthralgia, jaundice, and vaginal bleeding were more common in CHIKV positives but the difference between positives and nonpositives regarding these symptoms was not statistically significant. Dengue infections were associated with more frequent hospitalizations (OR = 8.38, 95% confidence intervals [CI] = 3.29-21.30) and mortality (OR = 19.0, 95% CI = 1.01-357.10). Hence, to conclude, in India wherever possible, all symptomatic pregnant women should be screened for DENV, CHIKV, and ZIKV as part of sentinel surveillance for ZIKV.

PMID:34607306 | DOI:10.4269/ajtmh.21-0584

Am J Trop Med Hyg. 2021 Oct 4:tpmd210785. doi: 10.4269/ajtmh.21-0785. Online ahead of print.

ABSTRACT

Occult Hepatitis B Infection (OBI) is a critical risk factor for triggering post-transfusion hepatitis (PTH), cirrhosis, hepatocellular carcinoma, and hepatitis B virus (HBV) reactivation, which β-thalassemia major (BTM) patients are at risk of it due to multiple blood transfusions. This study was aimed at determining the prevalence of OBI among BTM patients from Khuzestan Province, Iran. In this cross-sectional study, 90 thalassemia patients, who have received blood 36 to 552 times, participated referred to the Shafa hospital of Ahvaz city from January 2018 to April 2019. ELISA for determining serological markers (HBsAg, anti-HBc, anti-HBs, and anti-HCV) and real-time PCR for detecting HBV-DNA were performed; Nested PCR was conducted for DNA sequencing and determining the genotype of OBI case. Phylogenetic and statistical analyses were done by R package. Of 90 subjects enrolled in this study; 95.5% (86/90) were HBsAg negative, and the frequency of OBI among them was 1.16% (1/86). The anti-HBs, anti-HBc, and anti-HCV were detected in 80.00%, 7.78%, and 12.2% of patients, respectively. HBV-DNA was assessed at four HBsAg-positive subjects as well, and all of them were negative. The phylogenetic analysis showed that the detected HBV DNA in the OBI case belongs to the genotype D. This research, for the first time, demonstrated that OBI is present among β-thalassemia patients in Iran. Also, further studies are necessary to determine the actual prevalence of OBI among BTM patients in Iran to decisions concerning OBI screening, especially in transfusion centers.

PMID:34607305 | DOI:10.4269/ajtmh.21-0785

Nurse Educ Today. 2021 Sep 29;107:105160. doi: 10.1016/j.nedt.2021.105160. Online ahead of print.

ABSTRACT

BACKGROUND: Students use social media for sharing information and connecting with their friends, also for peer support, peer learning and student engagement. Research indicates that approximately twice the number of students were using social media for educational purposes compared to academic staff and almost all students discuss academic issues on social media. However, little is known about how diverse cohorts of student nurses use social media for specific purposes at different stages of their learning.

OBJECTIVES: Identify how student nurses in each country of study use social media for learning. Identify how each generation of student nurses use social media for learning. Identify how student nurses use social media as their education progresses.

DESIGN: A cross-sectional survey.

SETTINGS: The study was undertaken across three countries Jamaica, Trinidad and Tobago and the UK.

PARTICIPANTS: Student nurses from each of the countries that consented to participate met the inclusion criteria.

METHODS: 1050 student nurses across the three countries self-completed the cross-sectional survey between March and September 2019. Data was analysed using descriptive and inferential statistics.

RESULTS: WhatsApp® was the most used platform for learning amongst participants. Watching videos and downloading articles represented two-thirds of social media usage for learning. Smart phones were the most used device to access social media. Kruskal-Wallis tests were significant (≤0.001) for checking social media and messaging in lecture, use of social media for studies and classroom activities by country, generation (except classroom activities) and year of education. Use of social media for classroom activities had no significance by generation.

CONCLUSION: Country, generation and year of education are factors that influence the use of social media in student nurses’ learning. These should be considered by Universities in curriculum development and in teaching and learning delivery. From a pragmatic approach, social media is available and used by a majority of student nurses and can be widely assimilated into the nursing curriculum.

PMID:34607295 | DOI:10.1016/j.nedt.2021.105160

J Chromatogr A. 2021 Sep 22;1657:462570. doi: 10.1016/j.chroma.2021.462570. Online ahead of print.

ABSTRACT

The purpose of this paper is to propose a simple method for daily inspections of a gas chromatography-mass spectrometry (GC-MS) system with an instrumental detection limit (IDL) as an indicator. A definition of DLs by ISO is 3.3σ where σ denotes the standard deviation (SD) of blank measurements. Estimation of σ is carried out according to the function of mutual information (FUMI) theory and actually with commercial software (TOCO19). An IDL which is a combination of a signal area, width and noise level is concluded to be a good indicator for daily inspections compared with each of its constituents. Methyl stearate is used as a standard material for the daily inspection of a programmed-temperature GC-MS system. A short chromatogram of 1800 data points (1.5 min) containing a target signal and background noise is fit for the IDL prediction by TOCO19. The relative SDs (RSDs) based on the theoretically estimated σ are shown to coincide with statistical results from repeated measurements within 95% confidence intervals. Column temperature is observed to affect IDLs through background fluctuation and then temperature-IDL relationship is examined in a range from 170 to 270 °C. Actual daily inspections over a month are demonstrated.

PMID:34607291 | DOI:10.1016/j.chroma.2021.462570

Int J Med Inform. 2021 Sep 21;156:104588. doi: 10.1016/j.ijmedinf.2021.104588. Online ahead of print.

ABSTRACT

BACKGROUND: Electronic health record (EHR) data is commonly used for secondary purposes such as research and clinical decision support. However, reuse of EHR data presents several challenges including but not limited to identifying all diagnoses associated with a patient’s clinical encounter. The purpose of this study was to assess the feasibility of developing a schema to identify and subclassify all structured diagnosis codes for a patient encounter.

METHODS: To develop a subclassification schema we used EHR data from an interhospital transport data repository that contained complete hospital encounter level data. Eight discrete data sources containing structured diagnosis codes were identified. Diagnosis codes were normalized using the Unified Medical Language System and additional EHR data were combined with standardized terminologies to create and validate the subcategories. We then employed random forest to assess the usefulness of the new subcategorized diagnoses to predict post-interhospital transfer mortality by building 2 models, one using standard diagnosis codes, and one using the new subcategorized diagnosis codes.

RESULTS: Six subcategories of diagnoses were identified and validated. The subcategories included: primary or admitting diagnoses (10%), past medical, surgical or social history (9%), problem list (20%), comorbidity (24%), discharge diagnoses (6%), and unmapped diagnoses (31%). The subcategorized model outperformed the standard model, achieving a training AUROC of 0.97 versus 0.95 and testing model AUROC of 0.81 versus 0.46.

DISCUSSION: Our work demonstrates that merging structured diagnosis codes with additional EHR data and secondary data sources provides additional information to understand the role of diagnosis throughout a clinical encounter and improves predictive model performance. Further work is necessary to assess if subcategorizing produces benefits in interpreting the results of prognostic models and/or operationalizing the results in clinical decision support applications.

PMID:34607290 | DOI:10.1016/j.ijmedinf.2021.104588

Eur J Radiol. 2021 Sep 28;144:109978. doi: 10.1016/j.ejrad.2021.109978. Online ahead of print.

ABSTRACT

PURPOSE: The main goal of this systematic review was to assess the technical and clinical success, adverse events (AEs), surgery, and overall mortality proportion after percutaneous catheter drainage (PCD) of two pancreatic lesions.

METHODS: An extant search in online databases including Scopus, PubMed (Medline), Embase (Elsevier), Web of Science, Cochrane library, and Google Scholar, was conducted to recognize all studies that used PCD intervention in the management of pancreatic necrosis (PN) and pancreatic pseudocysts (PP). Random effects meta-analysis was performed, and Cochrane’s Q test and I²statistic were utilized to determine heterogeneity. In addition, meta-regression was used to explore the influence of categorical variables on heterogeneity.

RESULTS: Thirty-two studies (1398 patients) including PN in 26 (1256 cases, 89.8%) studies and PP in 6 (142 cases, 10.2%) studies were identified. Technical success proportion was 100% (95% confidence interval [CI] 100%-100%, I²: 0.0%), clinical success 63% (95% CI 55%-71%, I²: 92.9%), AEs 26% (95% CI 21%-31%, I²: 78%), surgery after PCD intervention 33% (95% CI 25%-40%, I²: 92.4%), and overall mortality was 13% (95% CI 9%-17%, I²: 82.8%). The most common ADs after PCD intervention were development of fistula (106, 42.6%), hemorrhage (44, 17.7%), sepsis (40, 16.1%).

CONCLUSION: A significant clinical success proportion with low AEs, surgery, and overall mortality proportion after PCD intervention was found, although the results should be interpreted with caution due to the high heterogeneity.

PMID:34607289 | DOI:10.1016/j.ejrad.2021.109978

ESMO Open. 2021 Oct 1;6(5):100279. doi: 10.1016/j.esmoop.2021.100279. Online ahead of print.

ABSTRACT

BACKGROUND: KRAS is mutated in ∼30% of non-small-cell lung cancer (NSCLC) but it has also been identified as one of the mechanisms underlying resistance to tyrosine kinase inhibitors (TKIs) in EGFR-positive NSCLC patients. Novel KRAS inhibitors targeting KRAS p.G12C mutation have been developed recently with promising results. The proportion of EGFR-positive NSCLC tumours harbouring the KRAS p.G12C mutation upon disease progression is completely unexplored.

MATERIALS AND METHODS: Plasma samples from 512 EGFR-positive advanced NSCLC patients progressing on a first first-line treatment with a TKI were collected. The presence of KRAS p.G12C mutation was assessed by digital PCR.

RESULTS: Overall, KRAS p.G12C mutation was detected in 1.17% of the samples (n = 6). In two of these cases, we could confirm that the KRAS p.G12C mutation was not present in the pre-treatment plasma samples, supporting its role as an acquired resistance mutation. According to our data, KRAS^G12C patients showed similar clinicopathological characteristics to those of the rest of the study cohort and no statistically significant associations between any clinical features and the presence of the mutation were found. However, two out of six KRAS^G12C tumours harboured less common EGFR driver mutations (p.G719X/p.L861Q). All KRAS^G12C patients tested negative for the presence of p.T790M resistance mutation.

CONCLUSIONS: The KRAS p.G12C mutation is detected in 1% of EGFR-positive NSCLC patients who progress on a first line with a TKI. All KRAS^G12C patients were negative for the presence of the p.T790M mutation and they did not show any distinctive clinical feature.

PMID:34607284 | DOI:10.1016/j.esmoop.2021.100279

J Biomech. 2021 Sep 27;129:110769. doi: 10.1016/j.jbiomech.2021.110769. Online ahead of print.

ABSTRACT

Although studies showed that several internal factors affect task-specific stability, the sex-specific effects of fatigue on whole-limb stability during a semi-cycle repetitive pointing task remain unidentified. Synergy and Motor Equivalence concepts in the UCM framework have been developed to explain task-specific stability. The motor equivalence model quantifies the amount of deviation in the space of elemental variables that occurs in two directions; one that preserves the performance variable (good variance), and the other that affects it (bad variance). Synergy index (the difference between good and bad variance divided by the total variance > 0) represent stability in performing a task. Healthy adults (n = 26, 13F; age: 35.3 ± 10.6 yrs.) performed an RPT by moving their dominant arm between a proximal target and a distal target in a standing position until near fatigue (Borg CR10 rating 8/10). Tridimensional kinematics of trunk, upper arm, forearm, and hand segments were captured by high-resolution cameras every minute, and joint angles were extracted according to the ZX’Y″ Euler sequence. Results showed the synergy > 0 for both women and men, reflecting synergies stabilizing the endpoint coordinate in both Non-Fatigue and Fatigue conditions. Statistics (ANOVA) showed a significant Condition * Sex effect (p = 0.01), with higher good (by 0.19 ± 0.1 rad) and bad variances (by 0.15 ± 0.09 rad) in women compared to men after fatigue. Higher good and bad variability, with no change in women’s performance could represent a less stable strategy, leading to the development of risk factors for neck-shoulder disorders.

PMID:34607280 | DOI:10.1016/j.jbiomech.2021.110769

Am J Otolaryngol. 2021 Sep 17;43(1):103214. doi: 10.1016/j.amjoto.2021.103214. Online ahead of print.

ABSTRACT

PURPOSE: Research indicates that most providers give opiates after endoscopic sinonasal surgery. The effectiveness of non-opiate medications after sinonasal surgery is poorly understood and most studies do not assess medication failure. This study compares oral opiate, oral opiate and topical steroid, and oral non-opiate pain control. Patient call-backs are used as a proxy for pain medication failure.

MATERIALS AND METHODS: This study compares three medication regiments after sinonasal surgery for 180 adults with chronic rhinosinusitis. Patients were instructed to take acetaminophen for mild pain. For moderate/severe pain, patients used: 1) oxycodone-acetaminophen, 2) oxycodone-acetaminophen + budesonide nasal rinses, or 3) meloxicam + acetaminophen. Patients were instructed to call clinic if pain was not controlled. Descriptive statistics compared cohorts. Chi-square tests compared call-backs between cohorts. Logistic regression adjusted for baseline differences in covariates, comorbidities, and operative sites.

RESULTS: Cohorts had similar age, sex distribution, disease features, and extent of surgery. The meloxicam cohort had less subjects with pain disorders. The oxycodone cohort had less subjects with diabetes, septoplasty, and turbinate reduction. After adjusting for baseline differences and using oxycodone as the reference group (n = 50), the odds of calling clinic for poorly controlled pain was 0.18 (95% Confidence Interval (CI): 0.05-0.6) in the meloxicam cohort (n = 45) and 0.19 (95% CI:0.07-0.5) in the oxycodone + budesonide rinses cohort (n = 85).

CONCLUSION: In this study, both meloxicam and oxycodone + budesonide rinses were more effective at controlling pain after sinonasal surgery than oxycodone alone.

PMID:34607277 | DOI:10.1016/j.amjoto.2021.103214

Psychoneuroendocrinology. 2021 Aug 14;133:105391. doi: 10.1016/j.psyneuen.2021.105391. Online ahead of print.

ABSTRACT

Salivary cortisol has been the central marker in psychoneuroendocrinological stress research for three decades. Given the technological possibilities to assess data in ecologically valid circumstances, many studies have implemented longitudinal assessments of salivary cortisol in study participants’ everyday life. Such studies bear the potential to understand real-life associations of cortisol with psychological traits, states, and health variables. Furthermore, changes in the neuroendocrine regulation and in cortisol reactivity can be used to evaluate the effects of behavioral interventions in real-life circumstances. While standardized paradigms have been developed to measure cortisol in laboratory settings, there is high heterogeneity in the assessment, statistical processing, and interpretation of everyday life cortisol measures. This methodological tutorial aims at summarizing important knowledge which had been accumulated during the past two decades and which could be used to set up an ambulatory assessment study focusing on salivary cortisol in everyday life. Practical advice for possible strategies at all stages of the research process is outlined in detail. Additionally, an example on how to statistically process cortisol data in a multilevel framework (including syntax) is provided. In these analyses, we investigate within- and between-person research questions regarding the association between stress and cortisol in daily life. Thus, the present work (a) can be used as tutorial for setting up everyday life studies focusing on the assessment of salivary cortisol, and (b) can be useful to avoid inconsistencies in study planning, data assessment and data processing in future studies.

PMID:34607270 | DOI:10.1016/j.psyneuen.2021.105391