Nevin Manimala

J Neurosurg. 2021 Feb 26:1-10. doi: 10.3171/2020.9.JNS201980. Online ahead of print.

ABSTRACT

OBJECTIVE: Primary central nervous system lymphoma (PCNSL) is a rare CNS tumor with a poor prognosis. It is usually diagnosed by needle biopsy and treated mainly with high-dose chemotherapy. Resection is currently not considered a standard treatment option. A possible prolonged survival after resection of PCNSL lesions in selected patients has been suggested, but selection criteria for surgery, especially for solitary lesions, have never been established.

METHODS: The authors retrospectively searched their patient database for records of adult patients (≥ 18 years) who were diagnosed and treated for a solitary PCNSL between 2005 and 2019. Patients were divided into groups according to whether they underwent resection or needle biopsy. Statistical analyses were performed in an attempt to identify variables affecting outcome and possible survival advantage and to characterize subgroups of patients who would benefit from resection of their tumor compared with undergoing biopsy only.

RESULTS: A total of 113 patients with a solitary lesion of PCNSL were identified; 36 patients underwent resection, and 77 had a diagnostic stereotactic biopsy only. The statically significant preoperative risk factors included age ≥ 70 years (adjusted HR 9.61, 95% CI 2.42-38.11; p = 0.001), deep-seated lesions (adjusted HR 3.33, 95% CI 1.13-9.84; p = 0.030), and occipital location (adjusted HR 4.26, 95% CI 1.08-16.78; p = 0.039). Having a postoperative Karnofsky Performance Scale (KPS) score < 80 (adjusted HR 3.21, 95% CI 1.05-9.77; p = 0.040) and surgical site infection (adjusted HR 4.27, 95% CI 1.18-15.47; p = 0.027) were significant postoperative risk factors after the adjustment and selection by means of other possible risk factors. In a subgroup analysis, patients younger than 70 years who underwent resection had a nonsignificant trend toward longer survival than those who underwent needle biopsy (median survival 35.0 months vs 15.2 months, p = 0.149). However, patients with a superficial tumor who underwent resection had significantly longer survival times than those who underwent needle biopsy (median survival 34.3 months vs 8.9 months, p = 0.014). Patients younger than 70 years who had a superficial tumor and underwent resection had significantly prolonged survival, with a median survival of 35.0 months compared with 8.9 months in patients from the same group who underwent needle biopsy (p = 0.007).

CONCLUSIONS: Specific subgroups of patients with a solitary PCNSL lesion might gain a survival benefit from resection compared with undergoing only a diagnostic biopsy.

PMID:33636699 | DOI:10.3171/2020.9.JNS201980

J Mech Behav Biomed Mater. 2021 Feb 13;117:104377. doi: 10.1016/j.jmbbm.2021.104377. Online ahead of print.

ABSTRACT

Osteogenesis imperfecta (OI), a brittle bone disease, is known to result in severe bone fragility. However, its ultrastructural origins are still poorly understood. In this study, we hypothesized that deficient intrafibrillar mineralization is a key contributor to the OI induced bone brittleness. To test this hypothesis, we explored the mechanical and ultrastructural changes in OI bone using the osteogenesis imperfecta murine (oim) model. Synchrotron X-ray scattering experiments indicated that oim bone had much less intrafibrillar mineralization than wild type bone, thus verifying that the loss of mineral crystals indeed primarily occurred in the intrafibrillar space of oim bone. It was also found that the mineral crystals were organized from preferentially in longitudinal axis in wild type bone to more randomly in oim bone. Moreover, it revealed that the deformation of mineral crystals was more coordinated with collagen fibrils in wild type than in oim bone, suggesting that the load transfer deteriorated between the two phases in oim bone. The micropillar test revealed that the compression work to fracture of oim bone (8.2 ± 0.9 MJ/m³) was significantly smaller (p < 0.05) than that of wild type bone (13.9 ± 2.7 MJ/m³), while the bone strength was not statistically different (p > 0.05) between the two genotype groups. In contrast, the uniaxial tensile test showed that the ultimate strength of wild type bone (50 ± 4.5 MPa) was significantly greater (p < 0.05) than that of oim bone (38 ± 5.3 MPa). Furthermore, the nanoscratch test showed that the toughness of oim bone was much less than that of wild type bone (6.6 ± 2.2 GJ/m³ vs. 12.6 ± 1.4 GJ/m³). Finally, in silico simulations using a finite element model of sub-lamellar bone confirmed the links between the reduced intrafibrillar mineralization and the observed changes in the mechanical behavior of OI bone. Taken together, these results provide important mechanistic insights into the underlying cause of poor mechanical quality of OI bone, thus pave the way toward future treatments of this brittle bone disease.

PMID:33636677 | DOI:10.1016/j.jmbbm.2021.104377

Water Res. 2021 Feb 15;194:116934. doi: 10.1016/j.watres.2021.116934. Online ahead of print.

ABSTRACT

Efficient management of sewer blockages requires increased preventive maintenance planning. Conventional approaches to the management of blockages in sewer pipe networks constitute largely unplanned maintenance stemming from a lack of adequate information and diagnosis of blockage causative mechanisms. This study mainly investigated a spatial statistical approach to determine the influence of explanatory factors on increased blockage propensity in sewers based on spatial heterogeneity. The approach consisted of the network K-function analysis, which provided an understanding of the significance of the spatial variation of blockages. A geographically-weighted Poisson regression then showed the degree of influence that explanatory factors had on increased blockage propensity in differentiated segments of the sewer pipe network. Lastly, blockage recurrence predictions were carried out with Random Forest ensembles. This approach was applied to three municipalities. Explanatory factors such as material type, number of service connections, self-cleaning velocity, sagging pipes, root intrusion risk, closed-circuit television inspection grade and distance to restaurants showed significant spatial heterogeneity and varying impacts on blockage propensity. The Random Forest ensemble predicted blockage recurrence with 60-80% accuracy for data from two municipalities and below 50% for the last. This approach provides knowledge that supports proactive maintenance planning in the management of blockages in sewer pipe networks.

PMID:33636665 | DOI:10.1016/j.watres.2021.116934

Nurse Educ Today. 2021 Feb 10;99:104811. doi: 10.1016/j.nedt.2021.104811. Online ahead of print.

ABSTRACT

BACKGROUND: Practice standards in nursing provide minimum expectations to enable the provision of high quality and safe care. There are currently no practice standards for post-registration graduate emergency nursing programs in Australia, leading to variation in graduate attributes and clinical expectations on completion of their program.

OBJECTIVES: The aim of this study was to establish consensus-based practice standards for graduate emergency nursing programs in Australia.

DESIGN: Delphi approach.

PARTICIPANTS: Australian nurses who identified as an emergency nurse and currently worked, or previously worked, in an emergency care environment.

METHODS: A modified two-round Delphi method was used. The survey was divided into four sections of data collection, including demographics, graduate emergency nursing course entry requirements, graduate expectations, and clinical care capabilities. Data were analysed using descriptive statistics including calculation of content validity index (CVI).

RESULTS: There were 204 respondents in Round One and 153 respondents in Round Two. Respondents agreed that nurses wanting to undertake graduate studies in emergency nursing require prior experience in the emergency care environment and should be working a minimum of 0.5 full time equivalent (FTE) whilst completing their studies. Thirty-nine statements presented under graduate attributes achieved a CVI of >0.8. All 70 clinical care capability statements presented in Round Two achieved a CVI of >0.8.

CONCLUSION: This study generated the evidence to establish minimum practice standards for Australian graduate emergency nursing programs. The standards centre around three key areas: graduate entry requirements, graduate attributes and clinical care capabilities. The standards provide a clear guide for employers, educators and clinicians, and inform capabilities for early career emergency nurses.

PMID:33636653 | DOI:10.1016/j.nedt.2021.104811

Nurse Educ Today. 2021 Feb 20;99:104818. doi: 10.1016/j.nedt.2021.104818. Online ahead of print.

ABSTRACT

BACKGROUND: With the constant urgent need to meet the demands of the future workforce, nursing education institutes are under increasing pressure to graduate more quality students. One way to achieve higher numbers of graduates would be to identify factors that predict nursing students’ academic success. No reports of such predictors were found for students in accelerated programs for non-nursing Bachelor’s degree graduates.

OBJECTIVES: This study was designed to examine the relationships between demographic characteristics, course grades in basic science and medical-surgical courses, and the final scores achieved by students in the Israel mandatory RN certification test.

DESIGN, SETTINGS, AND PARTICIPANTS: 164 students, enrolled in four courses of a second career in nursing program (accelerated program) in an academic nursing school in central Israel, participated in this retrospective study.

METHODS: Socio-demographic data and the final grades for basic science courses (chemistry and biochemistry, microbiology, anatomy and physiology, pathology, and pharmacology), the major Medical-Surgical course, and the RN certification test, were collected from the institutional database and analyzed.

RESULTS: Positive correlations were found between the mandatory RN certification test score, and the final grades of all the studies except the pathology course and Medical-Surgical course. The final RN certification test score was predicted by the final grades, with anatomy and physiology (p < 0.001), and pharmacology (p < 0.003), explaining 40% of the variance of the dependent variable. There were no statistical effects of demographic variables.

CONCLUSIONS: Early evaluation and prediction of academic success for nursing students in the second career program may guide effective interventions designed to promote students’ skills and improve their ability to complete the mandatory RN certification test successfully.

PMID:33636651 | DOI:10.1016/j.nedt.2021.104818

J Pharm Biomed Anal. 2021 Feb 15;197:113969. doi: 10.1016/j.jpba.2021.113969. Online ahead of print.

ABSTRACT

Previous studies have reported that nucleic acid methylation is a critical element in cardiovascular disease, and most studies mainly focused on sequencing and biochemical research. Here we developed an Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) method for the quantification analysis of the dissociative epigenetic modified nucleosides (5mdC, 5mrC, m⁶A) in Myocardial Infarction (MI) SD rats from different periods (1 week, 4 weeks, 8 weeks) after the surgery. The samples for analysis were obtained from heart tissue and blood of the rats. All the quantification results are compared with the sham-operated group. Total RNA and DNA were isolated by enzymatic hydrolytic methods before the UPLC-MS/MS analysis. The statistical analysis demonstrates the dynamic changes of modified nucleosides in MI rats, and it showed good specificity, accuracy, stability and less samples were needed in the method. In this paper, we discovered that the concentration of 5mdC, 5mrC, m⁶A from heart tissue significantly increased at 8 weeks after the surgery. Furthermore, UPLC-MS/MS helps us observe the similar change of the concentration of those 3 methylated biomarkers in peripheral blood after 8 weeks. The result shows that the dynamic process of those 3 methylated biomarkers in peripheral blood is related to the content of methylated biomarkers from the heart tissue. Based on the scientific evidence available, we proved that the methylation of genetic materials in peripheral blood is similar to myocardial infarction tissue. The relation between them indicates that peripheral blood could be a promising alternative to the heart tissue which monitor the level of methylation and MI diagnosis-aided.

PMID:33636646 | DOI:10.1016/j.jpba.2021.113969

J Environ Manage. 2021 Feb 23;286:112162. doi: 10.1016/j.jenvman.2021.112162. Online ahead of print.

ABSTRACT

The enhanced assessment of groundwater contamination vulnerability is necessary for the management and conservation of groundwater resources because groundwater contamination has been much increased continuously in the world by anthropogenic origin. The purpose of this study is to determine the best model among three ANFIS-MOA models (the adaptive neuro-fuzzy inference system (ANFIS) combined with metaheuristic optimization algorithms (MOAs) such as genetic algorithm (GA), differential evolution algorithm (DE) and particle swarm optimization (PSO)) in assessing groundwater contamination vulnerability at a nitrate contaminated area. The Miryang City of South Korea was selected as the study area because the nitrate contamination was widespread in the city with two functions of urban and rural activities. Eight parameters (depth to water, net recharge, topographic slope, aquifer type, impact to vadose zone, hydraulic conductivity and landuse) were classified into the numerical ratings on basis of modified DRASTIC method (MDM) for the input variables of ANFIS-MOA models. The Original ANFIS, and 3 combined models of ANFIS-PSO, ANFIS-DE and, ANFIS-GA used 95 adjusted vulnerability indices (AVI) as the target data of training (70% data) and testing (30% data) processing. The performance of 4 models was evaluated by mean absolute errors (MAE), root mean square errors (RMSE), correlation coefficients (R), ROC/AUC curves and predicted AVI (PAVI) maps. The statistical results, spatial vulnerability maps and correlation coefficients between PAVIs and nitrate concentrations revealed that the order of model excellence was ANFIS-PSO, ANFIS-DE, ANFIS-GA, and Original ANFIS, and that ANFIS-PSO showed the highest performance in training and testing processing. The performance rates of ANFIS-MOA models were also compared with 10 recent popular worldwide models using the correlation coefficients between PVI and nitrate concentrations, and they were superior to other recent popular models. ANFIS-MOA models were also useful for resolving the subjectivity of physical and hydrogeological parameters in original DRASTIC method (ODM) and MDM. It is expected that ANFIS-PSO models will produce the excellent results in assessing groundwater contamination vulnerability and that they can greatly contribute to the groundwater security in other areas of the world as well as Miryang City of South Korea.

PMID:33636625 | DOI:10.1016/j.jenvman.2021.112162

Eur J Obstet Gynecol Reprod Biol. 2021 Jan 29;259:95-99. doi: 10.1016/j.ejogrb.2021.01.054. Online ahead of print.

ABSTRACT

OBJECTIVE: During the 2020 COVID-19 pandemic there was a decrease in emergency room arrivals. There is limited evidence about the effect of this change in behavior on women’s health. We aimed to evaluate the impact of the COVID-19 pandemic on the diagnosis, treatment and complications of women presenting with a tubal Ectopic Pregnancy (EP).

STUDY DESIGN: This is a single centre retrospective cohort study. We compared the clinical presentation, treatment modalities and complications of all women presenting in our institution with a tubal EP during the COVID-19 pandemic between 15 March and 15 June 2020, with women who were treated in our institution with the same diagnosis in the corresponding period for the years 2018-2019.

RESULTS: The study group included 19 cases of EP (N = 19) that were treated between the 15 March 2020 and 15 June 2020. The control group included 30 cases of EP (N = 30) that were admitted to in the corresponding period during 2018 and 2019. Maternal age, parity, gravity and mode of conception (natural vs. assisted) were similar between the two groups. There was no difference in the mean gestational age (GA) according to the last menstrual period. In the study group more women presented with sonographic evaluation of high fluid volume in the abdomen than in the control group (53 % vs 17 %, P value 0.01). This finding is correlated with a more advanced disease status. In the study group there was a highly statistically significant 3-fold increase in rupture among cases (P < 0.005) and a 4-fold larger volume of blood in the entrance to the abdomen (P < 0.002). We found that there were no cases of ruptured EP in the group of women who were pregnant after assisted reproduction.

CONCLUSION: We found a higher rate of ruptured ectopic pregnancies in our institution during the COVID-19 pandemic. Health care providers should be alerted to this collateral damage in the non-infected population during the COVID-19 pandemic.

PMID:33636621 | DOI:10.1016/j.ejogrb.2021.01.054

Clin Neurophysiol. 2021 Feb 11;132(4):938-945. doi: 10.1016/j.clinph.2020.12.029. Online ahead of print.

ABSTRACT

OBJECTIVE: This investigation used magnetoencephalography (MEG) to identify the neurophysiological mechanisms contributing to the altered cognition seen in adults with cerebral palsy (CP).

METHODS: Adults with CP (GMFCS levels I-IV) and demographically-matched controls completed a Sternberg-type working memory task during MEG. Secondarily, they completed the National Institutes of Health (NIH) cognitive toolbox. Beamforming was used to image the significant MEG oscillatory responses and the resulting images were examined using statistical parametric mapping to identify cortical activity that differed between groups.

RESULTS: Both groups had a left-lateralized decrease in alpha-beta (11-16 Hz) power across the occipital, temporal, and prefrontal cortices during encoding, as well as an increase in alpha (9-13 Hz) power across the occipital cortices during maintenance. The strength of alpha-beta oscillations in the prefrontal cortices were weaker in those with CP during encoding. Weaker alpha-beta oscillation within the prefrontal cortex was associated with poorer performance on the NIH toolbox and a higher GMFCS level.

CONCLUSIONS: Alpha-beta aberrations may impact the basic encoding of information in adults with CP, which impacts their overall cognition. Altered alpha-beta oscillation might be connected with gross motor function.

SIGNIFICANCE: This experimental work highlights the aberrant alpha-beta during encoding as possible neurophysiological mechanism of the cognitive deficiencies.

PMID:33636609 | DOI:10.1016/j.clinph.2020.12.029

Econ Hum Biol. 2021 Feb 12;41:100988. doi: 10.1016/j.ehb.2021.100988. Online ahead of print.

ABSTRACT

In the U.S. in early 2020, heterogenous and incomplete county-scale data on COVID-19 hindered effective interventions in the pandemic. While numbers of deaths can be used to estimate actual number of infections after a time lag, counties with low death counts early on have considerable uncertainty about true numbers of cases in the future. Here we show that supplementing county-scale mortality statistics with socioeconomic data helps estimate true numbers of COVID-19 infections in low-data counties, and hence provide an early warning of future concern. We fit a LASSO negative binomial regression to select a parsimonious set of five predictive variables from thirty-one county-level covariates. Of these, population density, public transportation use, voting patterns and % African-American population are most predictive of higher COVID-19 death rates. To test the model, we show that counties identified as under-estimating COVID-19 on an early date (April 17) have relatively higher deaths later (July 1) in the pandemic.

PMID:33636583 | DOI:10.1016/j.ehb.2021.100988