Nevin Manimala

Psychol Trauma. 2021 Jun 28. doi: 10.1037/tra0001075. Online ahead of print.

ABSTRACT

Objective: Adverse childhood experiences are linked with poorer physical, social, and psychological well-being, especially for individuals who live in poverty. As adverse childhood experiences accumulate, risk for poor outcomes increases. Therefore, it is imperative that preschools and elementary schools are equipped to prevent and intervene upon traumatic stress. Trauma Smart is an organizational change intervention designed to build trauma-informed knowledge, attitudes, skills, and resources within schools serving young children. Method: The current study evaluates the effectiveness of Trauma Smart staff training in 42 preschools and elementary schools with 2,418 staff using a 1-year, longitudinal, prepost design. Trauma Smart implementation occurred during scale-up, under real world conditions. Satisfaction, posttraining knowledge about trauma-informed approaches, and pre-to-posttraining changes in attitudes favorable to trauma-informed care were evaluated. Results: As hypothesized, staff were highly satisfied with the training (mean ratings indicate 92% satisfied), demonstrated knowledge of core concepts related to trauma-informed care (mean quiz scores were scored 90% correct), and developed more favorable attitudes toward trauma-informed care following training, with medium-large effect sizes. Conclusions: Trauma Smart staff training is feasible, acceptable, and has the potential to improve the knowledge and attitudes relevant to trauma-informed approaches within preschool and elementary school staff, including those who serve children who live in poverty. (PsycInfo Database Record (c) 2021 APA, all rights reserved).

PMID:34180686 | DOI:10.1037/tra0001075

Minerva Med. 2021 Jun 28. doi: 10.23736/S0026-4806.21.07516-9. Online ahead of print.

NO ABSTRACT

PMID:34180641 | DOI:10.23736/S0026-4806.21.07516-9

Microbiologyopen. 2021 Jun;10(3):e1199. doi: 10.1002/mbo3.1199.

ABSTRACT

Vitamin B₁₂ (VB₁₂ ) is an indispensable cofactor of metabolic enzymes and has been widely used in the food and pharmaceutical industries. In this study, the effects of medium composition on VB₁₂ production by Propionibacterium freudenreichii were evaluated and optimized based on statistical experiments. The results showed that glucose, yeast extract, KH₂ PO₄ , and glycine have significant effects on VB₁₂ production. The final titer of VB₁₂ reached 8.32 ± 0.02 mg/L, representing a 120% increase over the non-optimized culture medium. We employed a metabolomics approach to analyze the differences of metabolite concentrations in P. freudenreichii cells cultivated in the original medium and optimized fermentation medium. Using multivariate data analysis, we identified a range of correlated metabolites, illustrating how metabolomics can be used to explain VB₁₂ production changes by corresponding differences in the overall cellular metabolism. The concentrations of many metabolic intermediates of glycolysis, the Wood-Werkman cycle, the TCA cycle, and amino acid metabolism were increased, which contributed to the synthesis of propionic acid and VB₁₂ due to an improved supply of energy and precursors.

PMID:34180597 | DOI:10.1002/mbo3.1199

ANZ J Surg. 2021 Jun 28. doi: 10.1111/ans.17045. Online ahead of print.

ABSTRACT

BACKGROUND: In our study, since the operative histopathological distinction of new malignant pulmonary lesions as either a primary lung cancer or a pulmonary metastasis is difficult, we aimed to identify the clinical variables which might allow distinction between a new lung cancer and a pulmonary metastasis, and the appropriate surgical management.

METHODS: We divided 55 cases into two groups: patients with new lung cancer (NLC, n = 32) and patients with pulmonary metastases (PM, n = 23). Based on the primary organ, the previous malignancy was classified into four categories: head and neck, colorectal, genitourinary, and breast cancer. The parameters included in the study were age, sex, smoking history, a family history of cancer, disease-free interval, primary organ, treatments for previous malignancies, size, and SUV max of the lung lesion on 18F-fluorodeoxyglucose positron emission tomography scan and high-resolution computed tomography findings of the lung lesion.

RESULTS: A predisposition for larger lesions was found in the NLC group. In addition, in the NLC group, disease-free interval was noted to be longer, patients were significantly older and SUV-max values of solitary pulmonary lesions were significantly higher than in the PM group. Pulmonary lesions in patients with prior head and neck cancers were more likely to develop NLC. No significant difference in statistical analysis was observed between the groups in terms of sex, smoking, a family history of cancer, a history of adjuvant therapy, radiological pulmonary lesions signs, and localization.

CONCLUSION: PL monitoring on CT surveillance is essential, particularly in patients with previous head and neck cancers, who appear to have a higher risk for NLC. If pathological MLN accompanies PL in a patient with previous extrapulmonary malignancy, cervical mediastinoscopy may help acquire a possible PL diagnosis besides mediastinal staging. Intraoperative frozen section may have difficulty in distinguishing between PM and NLC when the lesion is of the same histological type as the previous malignancy. When precise distinction cannot be achieved by frozen section, we speculate that DFI, age, and radiological findings of the PL may help thoracic surgeons take initiative peroperatively while designating the subsequent surgical intervention. Lastly, pulmonary segmentectomy is also better be considered along with lobectomy in NLC cases.

PMID:34180584 | DOI:10.1111/ans.17045

Acta Ophthalmol. 2021 Jun 28. doi: 10.1111/aos.14927. Online ahead of print.

ABSTRACT

PURPOSE: To estimate prevalence and incidence of diabetic retinopathy (DR) in a UK region by severity between 2012 and 2016 and risk factors for progression to proliferative DR (PDR).

METHODS: Electronic medical records from people with diabetes (PWD) ≥18 years seen at the Gloucestershire Diabetic Eye Screening Programme (GDESP) and the hospital eye clinic were analysed (HEC). Prevalence and incidence of DR per 100 PWD (%) by calendar year, grade and diabetes type were estimated using log-linear regression. Progression to PDR and associated risk factors were estimated using parametric survival analyses.

RESULTS: Across the study period, 35 873 PWD had at least one DR assessment. They were aged 66 (56-75) years (median (interquartile range)), 57% male, 5 (1-10) years since diabetes diagnosis, 93% Type 2 diabetes. Prevalence of DR decreased from 38.9% (95% CI: 38.1%, 39.8%) in 2012 to 36.6% (95% CI: 35.9%, 37.3%) in 2016 (p < 0.001). Incidence of any DR decreased from 10.9% (95% CI: 10.4%, 11.5%) in 2013 to 8.5% (95% CI: 8.1%, 9.0%) in 2016 (p < 0.001). Prevalence of PDR decreased from 3.5% (95% CI: 3.3%, 3.8%) in 2012 to 3.1% (95% CI 2.9%, 3.3%) in 2016 (p = 0.008). Incidence of PDR did not change over time. HbA_1c and bilateral moderate-severe NPDR were statistically significant risk factors associated with progression to PDR.

CONCLUSIONS: Incidence and prevalence of DR decreased between 2012 and 2016 in this well-characterized population of the UK.

PMID:34180581 | DOI:10.1111/aos.14927

Asia Pac J Clin Oncol. 2021 Jun 28. doi: 10.1111/ajco.13621. Online ahead of print.

ABSTRACT

AIMS: The Cancer Health Literacy – 30 (CHLT-30) and Cancer Health Literacy – 6 (CHLT-6) are widely used instruments to measure cancer health literacy and to identify individuals with limited cancer health literacy, respectively. This study evaluated the psychometric performance of both instruments in Chinese cancer patient population.

METHODS: The back-translation method was used in cross-cultural adaptation. Chinese versions, labeled as CHLT-30-Chinese and CHLT-6-Chinese, were administered to a diverse cross-sectional sample of 602 cancer patients. Of these, 30 patients retook the instruments over a 2-week interval to estimate test-retest reliability. Classical and modern psychometric methods were used to estimate the item and test properties.

RESULTS: Model fit statistics confirmed the original measurement structures of CHLT-30-Chinese (one-factor model) and CHLT-6-Chinese (latent class model with limited and adequate cancer health literacy classes) providing construct validity evidence. The CHLT-30-Chinese showed high internal consistency (Cronbach’s α = 0.83; McDonald’s Ω = 0.93) and 2-week test-retest reliability (r = 0.78). The CHLT-6-Chinese separated limited and adequate cancer health literacy classes well (entropy = 0.77) with the estimated prevalence rates of 19% and 81%, respectively. The CHLT-6-Chinese was highly precise in assigning cancer patients into two categories: limited and adequate cancer health literacy classes with probability of 0.95 and 0.90, respectively. Invariant measurement properties of both instruments were supported between male and female patients as well as patients with and without high school diploma.

CONCLUSIONS: The findings support the psychometric properties of the CHLT-30-Chinese for measuring cancer health literacy as a continuous trait and CHLT-6-Chinese for identifying patients with limited cancer health literacy with high precision. The two tools are recommended for use in both daily practice and clinical trials.

PMID:34180576 | DOI:10.1111/ajco.13621

J Anim Breed Genet. 2021 Jun 28. doi: 10.1111/jbg.12637. Online ahead of print.

ABSTRACT

Genomic information allows for a more accurate calculation of relationships among animals than the pedigree information, leading to an increase in accuracy of breeding values. Here, we used pedigree-based and single-step genomic approaches to estimate variance components and breeding values for β-hydroxybutyrate milk content (BHB). Additionally, we performed a genome-wide association study (GWAS) to depict its genetic architecture. BHB concentrations within the first 90 days of lactation, estimated from milk medium infrared spectra, were available for 30,461 cows (70,984 records). Genotypes at 42,152 loci were available for 9,123 animals. Low heritabilities were found for BHB using pedigree-based (0.09 ± 0.01) and genomic (0.10 ± 0.01) approaches. Genetic correlation between BHB and milk traits ranged from -0.27 ± 0.06 (BHB and protein percentage) to 0.13 ± 0.07 (BHB and fat-to-protein ratio) using pedigree and from -0.26 ± 0.05 (BHB and protein percentage) to 0.13 ± 0.06 (BHB and fat-to-protein ratio) using genomics. Breeding values were validated for 344 genotyped cows using linear regression method. The genomic EBV (GEBV) had greater accuracy (0.51 vs. 0.45) and regression coefficient (0.98 vs. 0.95) compared to EBV. The correlation between two subsequent evaluations, without and with phenotypes for validation cows, was 0.85 for GEBV and 0.82 for EBV. Predictive ability (correlation between (G)EBV and adjusted phenotypes) was greater when genomic information was used (0.38) than in the pedigree-based approach (0.31). Validation statistics in the pairwise two-trait models (milk yield, fat and protein percentage, urea, fat/protein ratio, lactose and logarithmic transformation of somatic cells count) were very similar to the ones highlighted for the single-trait model. The GWAS allowed discovering four significant markers located on BTA20 (57.5-58.2 Mb), where the ANKH gene is mapped. This gene has been associated with lactose, alpha-lactalbumin and BHB. Results of this study confirmed the usefulness of genomic information to provide more accurate variance components and breeding values, and important insights about the genomic determination of BHB milk content.

PMID:34180560 | DOI:10.1111/jbg.12637

J Forensic Sci. 2021 Jun 28. doi: 10.1111/1556-4029.14761. Online ahead of print.

ABSTRACT

Due to their medicolegal repercussions, forensic anthropology conclusions must be reliable, consistent, and minimally compromised by bias. Yet, a synthetic analysis of the reliability and biasability of the discipline’s methods has not yet been conducted. To do so, this study utilized Dror’s (2016) hierarchy of expert performance (HEP), an eight-level model aimed at examining intra- and inter-expert reliability and biasability (the potential for cognitive bias) within the literature of forensic science disciplines. A systematic review of the forensic anthropology literature was conducted (1972-present), including papers published in the American Journal of Physical Anthropology, Forensic Anthropology, Forensic Science International, and the Journal of Forensic Sciences and Anthropology Section abstracts published in the Proceedings of the Annual Meeting of the AAFS which matched keywords such as “forensic anthropology,” “bias,” “reliability,” “cognition,” “cognitive,” or “error.” The resulting forensic anthropology HEP showcases areas that have ample research and areas where more research can be conducted. Specifically, statistically significant increases in reliability (p < 0.001) and biasability (p < 0.001) publications were found since 2009 (publication of the NAS report). Extensive research examined the reliability of forensic anthropological observations and conclusions (n = 744 publications). However, minimal research investigated the biasability of forensic anthropological observations and conclusions (n = 20 publications). Notably, while several studies demonstrated the biasing effect of extraneous information on anthropological morphological assessments, there was no research into these effects on anthropological metric assessments. The findings revealed by the forensic anthropology HEP can help to guide future research, ultimately informing the development and refinement of best-practice standards for the discipline.

PMID:34180547 | DOI:10.1111/1556-4029.14761

J Med Virol. 2021 Jun 28. doi: 10.1002/jmv.27161. Online ahead of print.

ABSTRACT

OBJECTIVE: The role of human papillomavirus (HPV) in development of oral lesions is controversial. There is no comprehensive study about HPV prevalence in Iran. This systematic review and meta-analysis was aimed to find HPV prevalence of oral lesions and normal oral mucosa in Iran.

METHODS: International (PubMed, Web of Science and Scopus) and national (Iranmedex, Irandoc and SID) database were searched systematically until October 2020. Studies which examined prevalence of HPV in oral lesions by polymerase chain reaction method were included. The heterogeneity of articles was assessed with the Cochran Test and I-Square statistics. The prevalence rate of HPV was calculated using random effect model.

RESULTS: Of 3729 initial searched articles, 29 articles were eligible to include. Overall prevalence of HPV in oral lesions was 21%. The prevalence was highest in Rasht (50%) city. lip lesions had the highest HPV prevalence (40%). According to classification of lesions, the highest prevalence was in precancerous lesions (29%) and the lowest in normal mucosa (8%). Well-differentiated tumor showed higher prevalence than poorly-differentiated ones. The highest prevalence of HPV was in hairy leukoplakia (70%) and the lowest was in pyogenic granuloma (6%). Also, the prevalence was 31% in oral squamous cell carcinoma.

CONCLUSION: There are differences between HPV prevalence according to geographical area, intraoral location, type of lesion and grading. Since HPV prevalence was approximately high, further attention to vaccination and treatment for HPV in Iranian, as a potential risk factor for oral precancerous and cancerous lesions is recommended. This article is protected by copyright. All rights reserved.

PMID:34180541 | DOI:10.1002/jmv.27161

Phys Ther. 2021 Jun 26:pzab161. doi: 10.1093/ptj/pzab161. Online ahead of print.

ABSTRACT

OBJECTIVE: The Consolidated Standards of Reporting Trials (CONSORT) recommends reporting adverse events (AEs) and dropouts (DOs) with their definitions. The purpose of this study was to identify how AEs and DOs were reported in randomized controlled trials (RCTs) of therapeutic exercise for knee osteoarthritis (OA).

METHODS: Data sources were the Cochrane Library, EMBASE, PUBMED, and CINAHL. Databases were searched to identify RCTs of therapeutic exercise for Knee OA published from January 1, 1980, through July 23, 2020. Researchers independently extracted participant and intervention characteristics and determined whether a clear statement of and reasons for AEs and DOs existed. The primary outcome was exercise-related harm. Physiotherapy Evidence Database (PEDro) scoring described study quality and risk of bias. Descriptive and inferential statistics characterized results. Meta-analysis was not performed due to data heterogeneity.

RESULTS: One hundred 13 studies (152 arms) from 25 countries were included with 5909 participants exercising. PEDro scores ranged from 4 to 9. Exercise intensity was not specified in 57.9% of exercise arms. Fifty studies (44.2%) included an AE statement and 24 (21.2%) reported AEs, yielding 297 patients. One hundred three studies (91.2%) had a DO statement. Sixteen studies (15.5%) provided reasons for DOs that could be classified as AEs among 39 patients, yielding a 13.1% increase in AEs. Thus, 336 patients (6.0%) experienced exercise-related harm among studies with a clear statement of AEs and DOs. A significant difference existed in misclassification of DOs pre- and post-CONSORT 2010 (12.2% vs 3.1%; X21 = 21.2).

CONCLUSIONS: In some studies, the reason for DOs could be considered AEs, leading to potential underreporting of harm. Improvements in reporting of harm were found pre-and post-CONSORT 2010. Greater clarity regarding AE and DO definitions and TherEx intensity are needed to determine safe dosing and mode of therapeutic exercise for knee OA.

IMPACT: More adherence to the CONSORT statement is needed regarding reporting of and defining AEs, DOs, and therapeutic exercise intensity; however, despite this, therapeutic exercise seems to be associated with minimal risk of harm.

PMID:34180534 | DOI:10.1093/ptj/pzab161