Tag: nevin manimala

Microbiome. 2025 Dec 4. doi: 10.1186/s40168-025-02291-8. Online ahead of print.

ABSTRACT

BACKGROUND: Aflatoxin B₁ (AFB₁), a highly carcinogenic and hepatotoxic mycotoxin frequently contaminating animal feed, presents serious health risks to both humans and livestock. Although AFB₁‘s hepatotoxicity and other organ damage are extensively characterized, how this mycotoxin influences ruminal microbiota dynamics and functional activities in ruminants remains underexplored. Although some studies suggest that AFB₁ reduces nutrient digestibility and performance in ruminants, the underlying mechanisms are unclear. To aid in developing effective mitigation strategies for aflatoxicosis in ruminants, this study randomly divided Saanen goats into three groups. The CON group received the standard ration without additives, whereas LD and HD groups were provided identical basal diets fortified with 50 or 500 μg/kg AFB₁. Throughout the study, alterations in ruminal fermentation parameters, microbiome, and metabolome profiles were analyzed.

RESULTS: With increasing AFB₁ levels, ruminal pH, the concentration of total volatile fatty acids (VFA), acetate, and propionate decreased quadratically, while butyrate decreased linearly. Metagenomic profiling indicated suppressed populations of Pelagibacter and Flavobacterium following AFB₁ exposure, contrasting with promoted growth of Cryptobacteroides. Additionally, seven carbohydrate-active enzymes (CAZymes), specifically GT92, GT20, CE7, GT32, GT35, GT57, and GT50, were found to be more prevalent in the rumen of the CON group. Statistically higher viral loads characterized the HD group when benchmarked against CON group. Metabolomics analysis identified 1197 differential metabolites among the CON, LD, and HD groups, including cytochalasin Ppho and chrysophanol, both known for their teratogenic properties and their ability to induce cell death.

CONCLUSIONS: This study indicates that dietary AFB₁ exposure can alter the ruminal microbial and metabolomic profiles, induce prophage activation, and impact carbohydrate degradation and microbial protein turnover. These alterations may contribute to reductions in ruminal pH and volatile fatty acid concentrations, thereby impairing feed digestibility and animal performance. The findings provide valuable insights into AFB₁‘s effects on rumen health, and further investigations of these metabolic pathways may help develop precision interventions to mitigate AFB₁-induced rumen dysfunction and productivity losses. Video Abstract.

PMID:41345737 | DOI:10.1186/s40168-025-02291-8

BMC Psychol. 2025 Dec 5. doi: 10.1186/s40359-025-03767-w. Online ahead of print.

ABSTRACT

BACKGROUND: Healthcare workers are often exposed to traumatic events and as a result, they may experience post-traumatic stress disorder (PTSD) and other mental illnesses. The prevalence of these issues is high among healthcare professionals. Positive transformation (i.e., PTG) can emerge after struggles related to a stressful life event.

METHODS: In this cross-sectional study utilizing structural equation modeling (SEM), a sample of Iranian healthcare workers (nurses and pre-hospital emergency personnel from Ardabil) completed a battery of self-report questionnaires. These measures included a demographic information form, the Life Events Checklist (LEC-5), the Core Beliefs Inventory (CBI), the PTSD Checklist for DSM-5 (PCL-5), the Posttraumatic Growth Inventory (PTGI), scales assessing intrusive and deliberate rumination, and a social support questionnaire.

RESULTS: In examining the direct coefficient paths, all paths were statistically significant with the exception of the path of core belief disruption to deliberate rumination, social support to intrusive rumination, and PTSD to PTG. Similarly, in the examination of the coefficient of indirect paths, all paths were not statistically significant except for the paths of intrusive rumination to PTG through deliberate rumination and social support to PTG through deliberate rumination.

CONCLUSION: The study’s findings suggest that disruptions in core beliefs can result in both post-traumatic growth (PTG) and post-traumatic stress disorder (PTSD) and that social support plays a crucial role in promoting PTG. Clinical interventions that address disruptions in core beliefs and promote social support, such as mindfulness practices and purposeful thinking, may be effective in promoting mental health and well-being among healthcare professionals. Based on the results of the study, clinical interventions that address disruptions in core beliefs and promote social support may be effective in promoting mental health and well-being among healthcare professionals who have experienced traumatic events. As such, these findings can inform the development of evidence-based clinical interventions that can improve the mental health outcomes of healthcare professionals and, ultimately, enhance patient care.

PMID:41345734 | DOI:10.1186/s40359-025-03767-w

Eur J Med Res. 2025 Dec 4. doi: 10.1186/s40001-025-03593-1. Online ahead of print.

ABSTRACT

BACKGROUND: Diabetic ketoacidosis emergencies are serious acute complications of diabetes mellitus, and their health and economic impacts have been increasing among adult diabetic patients. Despite the growing burden of emergencies from diabetic ketoacidosis among adults with diabetes, its outcomes and factors of treatment have not been well studied in Ethiopia.

OBJECTIVE: To assess the prevalence of poor treatment outcomes of diabetic ketoacidosis and associated factors among adult diabetic patients admitted to the Debre Markos comprehensive specialized hospital, northwest Ethiopia.

METHODS: A hospital-based retrospective cohort study was conducted at Debre Markos comprehensive specialized hospital from April 8, 2025 to April 28, 2025. A total of 360 patients with diabetic DKA who were admitted from August 28, 2019 to September 30, 2024 participated in the study. A simple random sampling technique was used to select study participants. Data were extracted using a pre-tested data collection tool adapted from different literatures. The data were entered into epi-data version 4.6.0 and exported to SPSS version 26 for analysis. Both bivariable and multivariable binary logistic regression analysis were done. Statistical significance was declared at a p-value < 0.05 with an odds ratio of 95% confidence interval.

RESULTS: The prevalence of poor treatment outcomes was 15.5% (95%, CI: 11.5%, 19.3%). Factors, such as severe DKA (AOR: 1.482, 95% CI: 1.324, 4.872), the presence of comorbidities (AOR: 1.752, 95% CI: 1.215, 3.865), and underlying infections (95% CI: 1.362, 4.125), discontinuation of drugs (AOR: 2.115, 95% CI: 1.245, 3.865), treatment complications (AOR: 1.356, 95% CI: 1.253, 4.125), ketone levels > 3 (AOR: 1.213, 95% CI: 1.052, 2.876), and hospital stays of less than 5 days (AOR: 1.29, 95% CI: 1.022, 3.254) were also significant factors associated with poor outcomes in patients with DKA.

CONCLUSIONS: This study found that a high number of patients with DKA in the Debre Markos comprehensive specialized hospital experienced poor treatment outcomes. Significant factors included severe DKA, comorbidities, infections, drug discontinuation, treatment complications, high ketone levels and short hospital stays. To improve treatment outcomes, early identification and proactive management of high-risk DKA patients, particularly those who present with severe illness, comorbidity conditions, or infections, shall be prioritized.

PMID:41345728 | DOI:10.1186/s40001-025-03593-1

BMC Nutr. 2025 Oct 24;11(1):197. doi: 10.1186/s40795-025-01177-0.

NO ABSTRACT

PMID:41345726 | DOI:10.1186/s40795-025-01177-0

BMC Glob Public Health. 2025 Dec 5;3(1):111. doi: 10.1186/s44263-025-00229-8.

NO ABSTRACT

PMID:41345722 | DOI:10.1186/s44263-025-00229-8

Arthritis Res Ther. 2025 Dec 4. doi: 10.1186/s13075-025-03697-3. Online ahead of print.

ABSTRACT

OBJECTIVE: To evaluate maternal-fetal outcomes and therapeutic efficacy in Takayasu arteritis (TA)-complicated pregnancies through integrated retrospective analysis and meta-analytic synthesis.

METHODS: A dual-design study was conducted: (1) retrospective analysis of 20 pregnancies (17 patients) at West China Second Hospital (2012-2024), stratifying TA phases (acute/prolonged/stable); (2) systematic review with random-effects meta-analysis of 16 studies (568 pregnancies globally). Clinical data encompassed maternal-fetal profiles, TA-specific variables, laboratory metrics (hematologic/coagulation parameters), and therapies (glucocorticoids /immunosuppressants /antihypertensives). Outcomes were compared against normative standards using t-tests, Wilcoxon, chi-square, and meta-regression.

RESULTS: Among 20 pregnancies (median maternal age 28.5 years), 50% had at least one obstetric complication, with arterial stenosis (80%) and hypertension (40%) predominant. Meta-analysis revealed 42.6% adverse outcomes: gestational hypertension (26.1%), fetal growth restriction (17.7%), and preterm delivery (13.6%). Hematological analysis (n = 20) showed elevated WBC, PCT, TT, fibrinogen, urinary protein, and ALT (all P < 0.05), alongside reduced PT, albumin, and bilirubin (P < 0.05). Regarding the analysis results of inflammatory indicators, CRP (prepartum) (95%CI = 0.969-1.034, OR = 1.001), CRP (postpartum) (95%CI = 0.920-1.217, OR = 1.058), and ESR (95%CI = 0.952-1.101, OR = 1.024) showed no statistically significant association with pregnancy outcomes. Neither pre-pregnancy nor gestational glucocorticoids (prednisone vs methylprednisolone) or immunosuppressants significantly reduced complications (all RR 95% CI crossed 1; P > 0.05). Antihypertensive therapy showed no correlation with preeclampsia (P > 0.05).

CONCLUSION: TA significantly elevates maternal-fetal risks, driving hypertension, growth restriction, and preterm birth via vasculopathic-inflammatory pathways. Postpartum hypercoagulability (↑fibrinogen, ↓prothrombin time) necessitates multidisciplinary coagulation monitoring and mandatory thromboprophylaxis.

PMID:41345710 | DOI:10.1186/s13075-025-03697-3

BMC Psychol. 2025 Dec 4. doi: 10.1186/s40359-025-03780-z. Online ahead of print.

ABSTRACT

BACKGROUND: Serious complications affecting burn patients include reduced body image satisfaction, which can negatively influence their mental health. At the same time, resilience is a key factor in promoting psychological well-being. Therefore, the present study aimed to examine the relationship between body image satisfaction and resilience among burn patients in 2022-2023.

METHODS: In this descriptive cross-sectional study, 106 burn patients from Hamadan were enrolled using stratified quota sampling. The study instruments included a demographic information questionnaire, the Connor-Davidson Resilience Scale, and the Souto and Garcia Body Image Satisfaction Questionnaire. Data were analyzed using STATA version 14, employing descriptive statistics and Spearman’s correlation coefficient.

RESULTS: The results showed that the mean ± standard deviation for body image satisfaction was 59.83 ± 16.98, and for resilience, 56.26 ± 17.61. Furthermore, analysis indicated a strong, positive, and statistically significant correlation between body image satisfaction and resilience among these patients (r = 0.614, P < 0.001).

CONCLUSION: This study found that burn patients reported moderate levels of body image satisfaction and resilience. A significant positive correlation was observed between these two variables, suggesting that patients with higher body image satisfaction tend to exhibit greater resilience in coping with the physical and psychological challenges of burn injuries. These findings highlight the importance of integrating psychological care targeting body image into rehabilitation programs, as addressing body image concerns may help enhance resilience and improve overall well-being among burn survivors.

PMID:41345708 | DOI:10.1186/s40359-025-03780-z

BMC Sports Sci Med Rehabil. 2025 Dec 5. doi: 10.1186/s13102-025-01409-7. Online ahead of print.

ABSTRACT

OBJECTIVE: This study aimed to comparatively evaluate the scientific approach, accuracy, and practical applicability of different versions of ChatGPT in generating training programs.

METHOD: Adopting a mixed-methods design, the study employed seven distinct instruction sets, each developed with input from seven experts possessing a minimum of 10 years of professional experience (Certified Strength and Conditioning Specialists (CSCS), academicians holding PhDs in Sports Sciences with research focus on exercise physiology and training methodology) in their respective fields. Using these instruction sets, three versions of ChatGPT (ChatGPT-3.5, ChatGPT-4o, and ChatGPT-4.1) were tasked with generating 12-week resistance training programs for a hypothetical, healthy young adult male with a moderate training background. Using a rubric scoring scale, the generated programs were systematically evaluated and scored separately based on the following criteria: compliance with the initial program request, inclusion of literature references, adherence to exercise variety and progressive loading principles, individualization of progression and justification, program modifications, inclusion of warm-up and cool-down components, injury risk considerations, presence of incorrect recommendations and practical applicability, and accessibility.To determine whether differences in mean scores were statistically significant, the Friedman non-parametric test was applied. When significant differences were identified, pairwise comparisons were conducted using the Wilcoxon signed-rank test to determine which groups accounted for these differences. In addition, qualitative data analysis was performed to explore expert evaluations in depth, employing both content analysis and descriptive analysis techniques.

RESULTS: Statistically significant differences were identified among the ChatGPT versions examined in this study: between ChatGPT-4o and ChatGPT-3.5 (p = .018), ChatGPT-4.1 and ChatGPT-3.5 (p = .018), and ChatGPT-4.1 and ChatGPT-4o (p = .018). Expert content analysis further indicated that ChatGPT-4.1 produced responses that were more detailed, internally consistent, and better supported by scientific literature compared to the other versions.

CONCLUSION: Although the ChatGPT versions examined in this study exhibited certain limitations, they demonstrated the potential to deliver structured exercise programs aligned with established training principles and relevant scientific literature. Nonetheless, to ensure that AI-assisted training plans provide safe, evidence-based, and individualized content, the involvement of qualified human expertise remains essential.

TRIAL REGISTRATION: No official trial registration number was assigned.

PMID:41345701 | DOI:10.1186/s13102-025-01409-7

Trials. 2025 Dec 4;26(1):561. doi: 10.1186/s13063-025-09358-9.

ABSTRACT

BACKGROUND: The two-period randomised crossover design can be advantageous over the parallel-group randomised controlled design with two study arms, yielding greater statistical power and requiring smaller sample sizes. However, a general assumption of the crossover design is that study participants return to their stable baseline state after the experimental treatment has been withdrawn, either immediately or following a wash-out period.

MAIN BODY: In this article, we describe an alternative paradigm for the crossover design, which assumes that participants do not return to their baseline after the experimental treatment has discontinued-in other words, a paradigm under which a persistent carry-over effect is anticipated and even desired after intervention cessation. Such a paradigm is suitable, for example, when investigating behaviour change interventions that aim to establish long-lasting health behaviours through, for example, patient education or counselling. We present sample size calculations and statistical simulations to illustrate that under this alternative paradigm, the randomised crossover design can still maintain greater power than the parallel-group randomised controlled design. Statistical simulations show that, under realistic assumptions of partial or full carry-over, the crossover design can maintain equal or greater power than the parallel-group design, particularly when between-subject heterogeneity is non-negligible.

CONCLUSION: Trialists may consider this approach when the nature or intention of the experimental treatment is contrary to the assumption of return to baseline.

PMID:41345689 | DOI:10.1186/s13063-025-09358-9

Int J Health Geogr. 2025 Dec 4;24(1):39. doi: 10.1186/s12942-025-00431-9.

ABSTRACT

BACKGROUND: Equitable access to general practitioner services remains a persistent challenge for health systems and is critical for reducing health inequalities, particularly between urban and rural regions. Understanding the spatial and temporal dynamics of primary care provision is vital for informed healthcare planning and policy.

METHODS: Spatial and temporal disparities in the supply of general practitioners across Lower Saxony, Germany, were assessed over a 21-year period (2000-2021). Data from the Association of Statutory Health Insurance Physicians and municipal population statistics were used to develop the General Practitioner Regional Index – a composite measure incorporating both the availability and accessibility of general practitioners. Non-parametric statistical tests were applied to identify significant trends at the municipal level.

RESULTS: Pronounced geographic inequities in general practitioner supply were identified. More than 40% of the population resides in areas with declining supply, while only 5% experience improvements. Urban centres and their peripheries consistently exhibited higher and mostly stable levels of general practitioner supply. In contrast, rural small towns and villages demonstrated both lower baseline accessibility and more frequent negative trends. The primary driver of supply losses in affected areas was physician retirement, while relocation played a secondary role and typically occurred within similar regional types, thereby limiting redistribution effects.

CONCLUSIONS: The results underscore the persistence of urban-rural disparities in general practitioner availability and highlight physician retirements as the principal factor behind declining supply, with limited offsetting effects from physician migration. The findings indicate a need for spatially sensitive, succession-focused workforce strategies and innovative primary care models to mitigate rural undersupply and promote health equity.

TRIAL REGISTRATION: Not applicable. This study does not report the results of a health care intervention on human participants.

PMID:41345667 | DOI:10.1186/s12942-025-00431-9