Tag: nevin manimala

Int J Psychiatry Clin Pract. 2025 Mar 16:1-9. doi: 10.1080/13651501.2025.2476509. Online ahead of print.

ABSTRACT

OBJECTIVE: Cognitive Reactivity (CR) is the (re-)activation of negative cognitions by dysphoric mood. We examined whether CR predicts depressive episodes across 2 and 9 years, beyond subclinical depressive symptoms, neuroticism, and previous depressive episodes.

METHODS: Participants (N = 1,734) from the Netherlands Study of Depression and Anxiety (NESDA) were never-depressed or remitted-depressed for ≥1 month prior to baseline. We examined 2-year and 9-year predictions using Cox’s survival analysis and logistic regression, respectively. Two-year coefficient-based weight-points were calculated and evaluated using ROC analysis.

RESULTS: CR was a statistically-significant predictor of two-year depressive episodes, with an odds ratio of 1.04, 95% CI (1.02-1.06), and over nine years, with an adjusted hazard ratio of 1.01, 95% CI (1.01-1.02). The influence of CR and subclinical depressive symptoms decreased as the number of episodes increased, especially in ≥ 3 past episodes. Calculated weight-points correctly predicted 33.5% of participants who developed 2-year depression, compared to a 17.8% base rate (sensitivity = .81, specificity = .66).

CONCLUSIONS: CR is a moderately strong predictor of depressive episodes across 2 and 9 years. In participants with ≥ 3 prior episodes, depression history is such a strong predictor that a ceiling effect occurs, removing any added value of other predictors.

PMID:40089852 | DOI:10.1080/13651501.2025.2476509

Med Sci Monit. 2025 Mar 16;31:e946830. doi: 10.12659/MSM.946830.

ABSTRACT

BACKGROUND Pelvic floor dysfunction (PFD) significantly reduces quality of life. During horseback riding, the movement of the horse’s back affects the tension of the pelvic floor muscles. This study aimed to compare reported symptoms of pelvic floor dysfunction in women with past, current, and no history of horse show jumping. MATERIAL AND METHODS We examined 160 women (age 23.69±3.96 years). The cohort group consisted of 74 women training currently and training in the past, and a control group of 86 women not practicing horseback riding. The study used the original questionnaire and the Australian Pelvic Floor Questionnaire. RESULTS The analysis of the questionnaire results in groups (currently training, past training, control group) concerned bladder function (0.93±0.85 vs 0.88±0.66 vs 0.88±0.67, respectively; p=0.901), bowel function (1.22±0.96 vs 1.38±0.93 vs 1.37±0.77, respectively; p=0.266), pelvic organ prolapse (0.02±0.13 vs 0.00±0.00 vs 0.04±0.19, respectively; p=0.626), sexual activity (1.68±1.69 vs 1.46±1.23 vs 1.80±1.40, respectively; p=0.515), and total score for sexually active women (3.84±2.68 vs 3.82±1.86 vs 4.1±2.09, respectively; p=0.834). There were no statistically significant differences between the study groups in terms of individual PFD functions or overall PFD scores. CONCLUSIONS Pelvic floor dysfunction does not differ between show jumping riders and non-riders, so further research is advisable in this field, considering different equestrian sports and the use of clinical assessment of PFM function. Undoubtedly, the present study contributes to filling the knowledge gap and is important to the development of urogynaecology physiotherapy.

PMID:40089837 | DOI:10.12659/MSM.946830

Cryo Letters. 2025 Mar-Apr;46(2):108-115.

ABSTRACT

BACKGROUND: The impacts of suboptimal shipping conditions during transport on cell viability, recovery, and function of cryopreserved samples, have not been well studied.

OBJECTIVE: The impacts of suboptimal shipping on viability and recovery after the freezing and thawing were investigated using nine cancer cell lines, with particular reference to the approximate level of exposure temperature and exposure time at which adverse effects occur, and whether there are differences in sensitivity between cell types.

MATERIALS AND METHODS: The adverse effects of any set of suboptimal shipping conditions (-80 degree C for 7 d, -65 degree C or -50 degree C for 1, 3, and 7 d) on nine cancer cell lines (CHO-K1, COS-1, HeLa, HepG2, HL-60, Jurkat, MCF7, MDCK, 293T) were compared with data obtained during storage in liquid nitrogen.

RESULTS: No statistically significant decrease in viability was observed in seven of the nine cell lines after freezing and thawing. On the other hand, a statistically significant decrease in the cell recovery was observed after 2 d post freezing and thawing in the nine cell lines, except CHO-K1 at higher exposure temperatures and longer exposure times. Visualization of the adverse effects on the cell lines using a heat map showed that the impacts tended to be more pronounced under the condition of exposure at -50 degree C for three or more days.

CONCLUSION: These results will contribute to the development of standardized protocols and best practices for the optimal shipping of frozen animal cells. https://doi.org/10.54680/fr25210110212.

PMID:40089830

Cryo Letters. 2024 Nov-Dec;45(6):355-360.

ABSTRACT

BACKGROUND: Cryopreservation of spermatozoa is a biotechnology used for fertilization purposes and preservation of genetic material in various domestic species.

OBJECTIVE: To determine the efficacy of two different commercial semen diluents in the cryopreservation of epididymal semen of domestic cats.

MATERIALS AND METHODS: Twenty male cats aged between 1- 3 years and weighing 2.5- 4.5 kg were used in the study. The testicular tissues removed from the cats were immediately brought to the laboratory in physiological saline and the epididymal parts were trimmed in commercial semen extenders (INRA 96, Group I; OPTIXCELL, Group II). Diluted semen samples were cooled to 4°C and filled into 0.25 mL straws. Semen samples were frozen in a programmable semen freezing device and then placed in a liquid nitrogen container at -196 C. Semen samples were thawed at 38 degree C for 25 s. Thawed semen samples were evaluated in terms of motility and kinematic parameters using CASA.

RESULTS: No statistical difference was found between the groups in terms of total motility, progressive motility, and velocity parameters at 4 degree C. The rate of spermatozoa at slow speeds was found to be lower in group II. In addition, after freezing and thawing process, no statistical difference was observed between the groups in terms of motility, kinematics, and velocity parameters.

CONCLUSION: Both commercial semen extenders can be used for cryopreservation of cat epididymal semen. https://doi.org/10.54680/fr24610110712.

PMID:40089821

Cryo Letters. 2025 Jan-Feb;46(1):22-30.

ABSTRACT

BACKGROUND: Single sperm cryopreservation (SSC) is a method that preserves the limited number of spermatozoa in testicular sperm. But testicular spermatozoa are characterized with low movement, which is not ideal for sperm selection before SSC.

OBJECTIVE: This study was designed to investigate in vitro incubation (IVC) as a sperm selection technique before SSC on biological factors of testicular spermatozoa.

MATERIALS AND METHODS: Testicular tissue was obtained from 15 azoospermia men. One part of the testicular samples was used as a Control group, which was assessed fresh. One portion was cryopreserved by a vitrification (Vit) method and the two other portions were in vitro cultured for 24 h, with (IVC-Vit) or without (IVC) vitrification. Sperm motility, viability, morphology, DNA fragmentation and mitochondrial membrane potential were evaluated.

RESULTS: Sperm motility and viability were better maintained in the IVC-Vit group compared to the Vit group (P=0.04 and P= 0.003, respectively). Sperm morphology, the fresh, Vit, IVC, and IVC-Vit groups all showed similar results (P > 0.05). Mitochondrial activity was significantly lower in the Vit group compared to the Control fresh group (P = 0.0001). The IVC group had a significantly higher DFI as compared to the Control (P < 0.0001). Compared to the IVC group, the IVC-Vit sperm had a significant increase in DFI (P= 0.0009). There was a statistically significant difference between post warm DFI of the Vit group and IVC-Vit group (P < 0.0001).

CONCLUSION: IVC as a sperm selection method increased motility and viability of testicular spermatozoa before single sperm vitrification. As DNA fragmentation increased by this technique, this method is not ideal for selecting viable sperm. https://doi.org/10.54680/fr25110110512.

PMID:40089813

Cryo Letters. 2025 Jan-Feb;46(1):14-21.

ABSTRACT

BACKGROUND: Resveratrol (Res) (3,5,4′-trihydroxystilbene) is a natural polyphenol that exhibits important biological activities.

OBJECTIVE: To assess the effects of resveratrol (Res) on freeze-thawed survival of semen from Honamli and Hair Bucks.

MATERIALS AND METHODS: Six bucks, aged 2-3 years (three from each breed), were included in the study. Semen was collected from each breed and mixed separately after removing seminal plasma. The mixed semen was diluted with different Res concentrations (0 uM as control, 25 µM, 50 uM, 100 µM, 500 uM, and 1 mM) in Tris diluent and subjected to cryopreservation in liquid nitrogen vapor and frozen. After thawing, the samples were evaluated for motility and some spermatologic quality parameters by flow cytometry.

RESULTS: Data were analyzed separately for Honamli and Hair breeds. The results showed that the Res 1 mM group had the lowest motility in all assessments (P < 0.05). However, no significant differences were observed between the other Res and control groups (P > 0.05). In terms of apoptosis, Hair bucks exhibited a statistically significant difference in late apoptotic parameters, with the control showing the highest values (P. < 0.05). The Res 25 uM group (similar to the control group) showed lower mitochondrial oxidative stress than the Res 1 mM group (P. < 0.05).

CONCLUSION: Res at a dose of 1 mM did not protect most sperm functional and biochemical parameters except for apoptosis and performed worse than the control group. When all parameters are evaluated collectively, concentrations lower than 1 mM should be used for freezing Honamli and Hair Buck semen with resveratrol. https://doi.org/10.54680/fr25110110312.

PMID:40089812

Lipids Health Dis. 2025 Mar 15;24(1):94. doi: 10.1186/s12944-025-02518-5.

ABSTRACT

BACKGROUND: The relationship between the triglyceride glucose (TyG) values and the development of diabetes in non-alcoholic fatty liver disease (NAFLD) patients is not yet well researched. This study aims to examine how the baseline TyG levels correlate with the incidence of new-onset diabetes in this specific cohort.

METHODS: This cohort included 2,506 normoglycemic Japanese adults with NAFLD who underwent routine health check-ups at Murakami Memorial Hospital between 2004 and 2015. Several statistical approaches, including restricted cubic splines and two-piecewise linear regression, were utilized to assess the relation between the TyG levels and diabetes risk.

RESULTS: Among the 2,506 participants (mean age: 44.78 ± 8.32 years; 81.09% male), 203 individuals (8.10%) developed diabetes over the course of the 11-year follow-up period. A U-shaped relationship was observed between the levels of TyG and the onset of diabetes, with an inflection point identified at a TyG value of 7.82 (95% CI: 7.72-8.00). Below this threshold, each one-unit elevation in TyG values reduced the probability of diabetes by 93% (HR = 0.07, 95% CI: 0.01-0.32, P = 0.001). Conversely, above this threshold, each one-unit elevation increased the probability of diabetes by 70% (HR = 1.70, 95% CI: 1.19-2.44, P = 0.004).

CONCLUSIONS: The findings validate a U-shaped association between TyG levels and new-onset diabetes in adults with NAFLD. Both low and high TyG levels increase diabetes probability in such a group.

PMID:40089802 | DOI:10.1186/s12944-025-02518-5

Nutr J. 2025 Mar 15;24(1):43. doi: 10.1186/s12937-025-01099-4.

ABSTRACT

BACKGROUND: Diet is a modifiable risk factor for non-communicable diseases which are the major causes of death worldwide. French dietary guidelines, updated in 2017, provide recommendations for a healthier diet. We aimed to study the association between adherence to these dietary guidelines and mortality in the E3N (Etude Epidémiologique auprès de femmes de l’Education Nationale) French cohort. A secondary objective was to investigate the role of dietary exposure to chemical contaminants in this association.

METHODS: We studied 72 585 women of the E3N prospective cohort, which completed a food frequency questionnaire in 1993. We estimated adherence to French dietary guidelines using the simplified “Programme National Nutrition Santé-guidelines score 2” (sPNNS-GS2, range -20.4 to 12.6). We estimated the association between sPNNS-GS2 and all-cause or cause-specific mortality using Cox proportional hazard models. Causes of death were coded and validated by the French Epidemiology Center on Medical Causes of Death (Inserm-CépiDc).

RESULTS: During follow-up (1993-2014), we identified 6 441 deaths. The mean sPNNS-GS2 was 3.8 (SD 3.0). In the fully adjusted model, we found a non-linear association between sPNNS-GS2 and all-cause, all-cancer, breast cancer and lung cancer mortality (p-values for the overall association < 0.001), with a diminution of the risk as sPNNS-GS2 increases up to its median or 65^th percentile (depending on the outcome), and then a plateau (for all-cause and breast cancer mortality) or an inversion of the trend (for all-cancer and lung cancer mortality). Furthermore, we identified a linear inverse association with cardiovascular diseases mortality (HR_oneSTD [95%CI]: 0.86 [0.76; 0.97]), and no association with colorectal cancer mortality. We observed similar results when additionally adjusting on dietary exposure to chemical contaminants.

CONCLUSIONS: This study conducted in a large prospective cohort following more than 70 000 women for over 20 years suggested that higher adherence to French dietary guidelines was associated with a reduced risk of mortality from all-cause, cardiovascular diseases, all-cancer, breast cancer, and lung cancer, except for high values of adherence for lung cancer mortality. These results contribute to informing on the importance of following the French nutritional recommendations.

PMID:40089799 | DOI:10.1186/s12937-025-01099-4

BMC Oral Health. 2025 Mar 15;25(1):390. doi: 10.1186/s12903-025-05745-5.

ABSTRACT

BACKGROUND: Effective and durable bonding of immediate dentin sealing (IDS) is a crucial factor in the success of adhesive-bonded restorations. This study aimed to investigate the influence of chlorhexidine (CHX) and benzalkonium chloride (BAC) pretreatments on the immediate and aged bond strengths of a universal adhesive used in IDS across two etching modes.

METHODS: Flat dentin surfaces were obtained from 120 molars and divided into six groups based on pretreatments (no treatment, CHX, and BAC) in two etching modes (etch-and-rinse (E&R) and self-etch (SE)) using All Bond Universal, in conjunction with a hydrophobic resin layer. Following the provisional stage and resin cementation, immediate and aged shear bond strengths (SBS) were measured after 24 h and 1 year of storage, respectively. The data (in MPa) were analyzed using ANOVA and Tukey tests (p < 0.05).

RESULTS: CHX pretreatment did not significantly impact bond strengths, while BAC pretreatment led to a significantly lower immediate SBS in the SE mode (9.91 ± 1.93 MPa) compared to the control group (16.07 ± 3.45 MPa, p < 0.001). In the E&R mode, no statistically significant differences were found among the three pretreatment groups at either storage time (p > 0.05). After one year, SBS values in the SE mode decreased for all groups, with the CHX group showing the lowest value (9.98 ± 3.91 MPa), though these reductions were not statistically significant (p > 0.05). In contrast, SBS values in the E&R mode remained stable or slightly increased, with the BAC group reaching 17.12 ± 4.05 MPa after one year. Aged SBS values in the E&R mode were significantly higher than in the SE mode for the CHX (16.53 ± 2.28 MPa vs. 9.98 ± 3.91 MPa, p < 0.001) and BAC groups (17.12 ± 4.05 MPa vs. 10.55 ± 3.03 MPa, p = 0.001).

CONCLUSION: CHX and BAC pretreatments did not offer additional advantages in terms of long-term bonding performance in IDS with the universal adhesive utilized in this study. When using antibacterial agents, All Bond Universal adhesive in E&R mode could provide better long-term bonding performance than that of its SE mode.

PMID:40089794 | DOI:10.1186/s12903-025-05745-5

Breast Cancer Res. 2025 Mar 15;27(1):39. doi: 10.1186/s13058-025-01989-9.

ABSTRACT

BACKGROUND: Currently, the primary methods for detecting HER2 expression levels are immunohistochemistry (IHC) and in situ hybridization (ISH), with the traditional standard being a HER2-positive score of 3 + accompanied by ERBB2 gene amplification detected through ISH. However, a new entity has recently emerged: HER2-low, defined as HER2 IHC 1 + or 2 + with negative ISH. HER2-low breast cancer, representing 45-60% of all HER2-negative tumors, has distinct biological characteristics and uncertain responses to conventional HER2-targeted therapies. Recent studies suggest varied clinical outcomes, highlighting the need for further investigation into the impact of HER2-low status on treatment efficacy and prognosis.

OBJECTIVE: This meta-analysis evaluates the difference in complete pathological response (pCR), disease-free survival (DFS), and overall survival (OS) between HER2-low and HER2-zero phenotypes.

METHODS: We systematically searched the main databases PubMed, Scopus, and Web of Science for articles evaluating women in neoadjuvant therapy expressing HER2-low and HER2-zero. We computed odds ratios (ORs) or hazard ratios (HRs) using DerSimonian and Laird random-effect models for all endpoints, with 95% confidence intervals (CIs). We assessed the heterogeneity using I² statistics. R, version 4.2.3, was used for statistical analyses.

RESULTS: 38 studies totaling 70,104 patients were included. The HER2-low group accounted for 61.3% of patients while HR + status represented 52.4% in the whole research. In 67,839 women, the pCR was analyzed, which in the overall cohort analysis favored the HER2-zero group (OR 0.84; 95% CI 0.78-0.90; p = 0.000005; I² = 15%). Subgroup analyses for triple-negative breast cancer (TNBC) and HR + patients also favored HER2-zero expression, with an OR of 0.91 (95% CI 0.83-1.0; p < 0.041; I² = 12%) and 0.75 (95% CI 0.70-0.81; p < 0.000001; I² = 0%), respectively. In the multivariate analysis across all patients, both DFS and OS outcomes were significantly favorable for the HER2-low expression group, with HR 0.8317 (95% CI 0.7036-0.9832; p = 0.031) for DFS and HR 0.806 (95% CI 0.663-0.979; p = 0.03) for OS.

CONCLUSION: Based on our findings, HER2-zero status is associated with a significantly higher pathological complete response (pCR) rate compared to HER2-low in early-stage breast cancer, and other survival outcomes. These results suggest that HER2-zero should be considered a prognostic factor in early-stage breast cancer and taken into account in neoadjuvant treatment planning and future clinical research.

PMID:40089780 | DOI:10.1186/s13058-025-01989-9