Tag: nevin manimala

Cancer Med. 2022 Feb 16. doi: 10.1002/cam4.4584. Online ahead of print.

ABSTRACT

BACKGROUND: Metabolic dysfunction and inflammation have been associated with endometrial cancer risk; however, their influence on endometrial cancer survival is less understood.

METHODS: A prospective cohort study of 540 endometrial cancer cases diagnosed between 2002 and 2006 in Alberta were followed for survival outcomes to 2019. Baseline blood samples collected either pre- or post-hysterectomy were analyzed for glucose, insulin, adiponectin, leptin, tumor necrosis factor-α, interleukin-6, and C-reactive protein. Covariates were obtained during in-person interviews and via medical chart abstraction. Cox proportional hazard regression models were used to estimate multivariable-adjusted hazard ratios (HR) and 95% confidence intervals (95% CI) for the association between each biomarker and disease-free and overall survival.

RESULTS: Blood samples were collected from 520 of the 540 participants (presurgical n = 235; postsurgical n = 285). During the median follow-up of 14.3 years (range 0.4-16.5 years), there were 125 recurrences, progressions, and/or deaths with 106 overall deaths. None of the biomarkers were associated with disease-free or overall survival in multivariable-adjusted analyses. In an exploratory stratified analysis, the highest level of presurgical adiponectin, compared to the lowest level, was associated with improved disease-free (HR = 0.42, 95% CI = 0.20-0.85) and overall (HR = 0.41, 95% CI = 0.18-0.92) survival, whereas no statistically significant associations were noted for postsurgical measures of adiponectin.

CONCLUSIONS: Overall, there was no evidence of an association between biomarkers of insulin resistance and inflammation with mortality outcomes in endometrial cancer survivors. Future cohort studies with serial blood samples are needed to understand the impact of changes in insulin resistance and inflammatory markers on endometrial cancer survival.

PMID:35174651 | DOI:10.1002/cam4.4584

Orthop Surg. 2022 Feb 17. doi: 10.1111/os.13230. Online ahead of print.

ABSTRACT

OBJECTIVE: To explore the feasibility and clinical efficacy of a modified medial collateral ligament indentation technique in total knee arthroplasty (TKA) with severe type II valgus deformity.

METHODS: Consecutive patients with Krackow type II valgus deformity >20° who underwent a primary unilateral TKA between May 2008 and June 2017 were studied retrospectively. A medial collateral ligament indentation technique was performed in 20 patients (MCLI group), and 23 patients received the routine lateral structures release technique (LSR group). Radiological parameters, such as the valgus angle (VA), and functional outcomes including the use of constraint implants, Knee Society Score (KSS), Knee Society Function score (KSF), and thickness of the polyethylene insert were compared between the two groups.

RESULTS: A total of 43 consecutive patients had a minimum 2-year follow-up. The preoperative VA was comparable between the MCLI (23.5° ± 5.8°) and LSR groups (21.3° ± 3.2°, P = 0.134), as was the postoperative VA (1.1° ± 2.1° and 2.5° ± 3.0°, respectively, P = 0.084). The mean KSS and KSF scores in the MCLI group were 30.2 ± 4.8 and 38.8 ± 4.8, respectively, before surgery, and they increased to 91.3 ± 2.6 and 86.5 ± 2.4 at the last follow-up. The scores in the LSR group were 31.5 ± 7.5 and 36.5 ± 7.8 before surgery and 92.4 ± 3.5 and 88.5 ± 3.6 at the last follow-up. While no statistically significant differences in pre- or postoperative functional scores were found between the two groups, the MCLI group had thinner polyethylene inserts (9.5 ± 1.1 mm vs 12.9 ± 1.5 mm) and less use of constrained condylar inserts (15% vs 69.6%). During follow-up, the MCLI group had fewer complications.

CONCLUSION: A modified MCLI technique can achieve good outcomes in TKA with type II valgus deformity of >20°. It can maintain a normal joint line level, reduce the use of constrained condylar knee prostheses, and is a reliable choice for severe genu valgum.

PMID:35174652 | DOI:10.1111/os.13230

Acta Ophthalmol. 2022 Feb 17. doi: 10.1111/aos.15115. Online ahead of print.

ABSTRACT

PURPOSE: The aim of our study was to compare metabolic (oxygen saturation; %) and anatomical (diameter; μm) retinal vessel parameters of patients with central serous chorioretinopathy (CSC) to those of controls.

METHODS: In this prospective cross-sectional cohort study, 72 eyes of patients with CSC were compared with 21 eyes of healthy controls. Of the 72 patients, 52 had chronic, nonactive CSC (subgroup nCSC) and 20 had active CSC (subgroup aCSC), according to activity on fluorescein angiography. Retinal vessel oximetry (RO) was performed using the Oxymap T1 oximeter. Oxygen saturation in all major peripapillary retinal arterioles (A-SO₂ ) and venules (V-SO₂ ) was measured, and their difference (A-V SO₂ ) was calculated. In addition, we evaluated the corresponding diameter in retinal arterioles (D-A) and venules (D-V). For statistical evaluation, ANOVA-based linear mixed-effects models were calculated (SPSS®; p < 0.05).

RESULTS: Central serous chorioretinopathy (CSC) patients had significantly higher A-SO₂ and V-SO₂ compared to that of controls (p = 0.031 and p = 0.018 respectively). Especially, the subgroup of aCSC patients showed significantly higher A-SO₂ and V-SO₂ values (p = 0.027 and p = 0.034, respectively). In addition, superotemporal and superonasal quadrant location showed significant interactions with A-SO₂ and V-SO₂ (p ≤ 0.03). Diameter in retinal arterioles (D-A), an venules (D-V) and A-V SO₂ findings showed no significant differences (p > 0.096).

CONCLUSION: These data indicate that patients with CSC have altered metabolic function. The presence of disease activity showed the greatest influence on RO measurement, both compared to controls and to those with inactive chronic CSC disease.

PMID:35174647 | DOI:10.1111/aos.15115

Can Rev Sociol. 2022 Feb 16. doi: 10.1111/cars.12375. Online ahead of print.

ABSTRACT

The rise of the Big Data paradigm has made it more feasible to track how personal networks evolve on social media, where auto-generated contact records and fine-grained temporal data sequences help capture how and when interpersonal ties and contacts change their roles. Using a sample of matched survey data and social media records, we investigated the mechanisms by which indirect contacts (“degree-2 alters”) transform into direct contacts (“degree-1 alters”) from a Facebook user’s (ego’s) point of view. To highlight the temporal sequences, we assigned different roles to the same alters depending on how each of them is connected with ego at different periods of time. Multilevel event history analyses pinpoint several online actions and network features of ego, degree-1 alters, and degree-2 alters, as the key factors that contribute to the transformation from indirect contacts into direct contacts.

PMID:35174632 | DOI:10.1111/cars.12375

J Clin Hypertens (Greenwich). 2022 Feb 17. doi: 10.1111/jch.14431. Online ahead of print.

ABSTRACT

Severe hypertension (HTN) that develops during hospitalization is more common than admission for HTN; however, it is poorly studied, and treatment guidelines are lacking. Our goal is to characterize hospitalized patients who develop severe HTN and assess blood pressure (BP) response to treatment. This is a multi-hospital retrospective cohort study of adults admitted for reasons other than HTN who developed severe HTN. The authors defined severe inpatient HTN as the first documented BP elevation (systolic BP > 180 or diastolic BP > 110) at least 1 hour after admission. Treatment was defined as receiving antihypertensives (intravenous [IV] or oral) within 6h of BP elevation. As a measure of possible overtreatment, the authors studied the association between treatment and time to mean arterial pressure (MAP) drop ≥ 30% using the Cox proportional hazards model. Among 224 265 hospitalized adults, 10% developed severe HTN of which 40% were treated. Compared to patients who did not develop severe HTN, those who did were older, more commonly women and black, and had more comorbidities. Incident MAP drop ≥ 30% among treated and untreated patients with severe HTN was 2.2 versus 5.7/1000 person-hours. After adjustment, treated versus. untreated patients had lower rates of MAP drop ≥ 30% (hazard rate [HR]: 0.9 [0.8, 0.99]). However, those receiving only IV treatment versus untreated had greater rates of MAP drop ≥ 30% (1.4 [1.2, 1.7]). Overall, the authors found that clinically significant MAP drop is observed among inpatients with severe HTN irrespective of treatment, with greater rates observed among patients treated only with IV antihypertensives. Further research is needed to phenotype inpatients with severe HTN.

PMID:35174627 | DOI:10.1111/jch.14431

Biol Rev Camb Philos Soc. 2022 Feb 16. doi: 10.1111/brv.12840. Online ahead of print.

ABSTRACT

Mercury contamination is a major threat to the global environment, and is still increasing in some regions despite international regulations. The methylated form of mercury is hazardous to biota, yet its sublethal effects are difficult to detect in wildlife. Body condition can vary in response to stressors, but previous studies have shown mixed effects of mercury on body condition in wildlife. Using birds as study organisms, we provide the first quantitative synthesis of the effect of mercury on body condition in animals. In addition, we explored the influence of intrinsic, extrinsic and methodological factors potentially explaining cross-study heterogeneity in results. We considered experimental and correlative studies carried out in adult birds and chicks, and mercury exposure inferred from blood and feathers. Most experimental investigations (90%) showed a significant relationship between mercury concentrations and body condition. Experimental exposure to mercury disrupted nutrient (fat) metabolism, metabolic rates, and food intake, resulting in either positive or negative associations with body condition. Correlative studies also showed either positive or negative associations, of which only 14% were statistically significant. Therefore, the overall effect of mercury concentrations on body condition was null in both experimental (estimate ± SE = 0.262 ± 0.309, 20 effect sizes, five species) and correlative studies (-0.011 ± 0.020, 315 effect sizes, 145 species). The single and interactive effects of age class and tissue type were accounted for in meta-analytic models of the correlative data set, since chicks and adults, as well as blood and feathers, are known to behave differently in terms of mercury accumulation and health effects. Of the 15 moderators tested, only wintering status explained cross-study heterogeneity in the correlative data set: free-ranging wintering birds were more likely to show a negative association between mercury and body condition. However, wintering effect sizes were limited to passerines, further studies should thus confirm this trend in other taxa. Collectively, our results suggest that (i) effects of mercury on body condition are weak and mostly detectable under controlled conditions, and (ii) body condition indices are unreliable indicators of mercury sublethal effects in the wild. Food availability, feeding rates and other sources of variation that are challenging to quantify likely confound the association between mercury and body condition in natura. Future studies could explore the metabolic effects of mercury further using designs that allow for the estimation and/or manipulation of food intake in both wild and captive birds, especially in under-represented life-history stages such as migration and overwintering.

PMID:35174617 | DOI:10.1111/brv.12840

J Cosmet Dermatol. 2022 Feb 16. doi: 10.1111/jocd.14854. Online ahead of print.

ABSTRACT

BACKGROUND: Pemphigus is a series of autoimmune skin disorders caused by IgG. Regulatory T cells (Tregs) are a subset of CD4+ T cells that mostly block pathogenic immune responses mediated by self-reactive cells, therefore a lack of Tregs or a malfunction in their activity could lead to a loss of tolerance and the development of autoimmunity. .

AIMS: to evaluate the expression of lesional and perilesional Treg markers (CD4+ CD25+ bright FOXP3+) in pemphigus patients.

PATIENTS AND METHODS: Twenty three pemphigus patients and 20 healthy controls were included in this study. The expression of CD4, CD25 and Foxp3 were evaluated by immunohistochemistry.

RESULTS: There was statistically significant increase in CD4+ T lymphocytes in lesional skin of pemphigus compared to perilesional skin and control group (P-value: 0.001). There was statistically significant decrease in CD25+ and Foxp3+ cells in lesional skin compared to perilesional and control group (P-value: <0.001, 0.025 respectively ).

CONCLUSION: The reduction of lesional skin Tregs may play an important role in the pemphigus pathogenesis.

PMID:35174611 | DOI:10.1111/jocd.14854

J Cosmet Dermatol. 2022 Feb 16. doi: 10.1111/jocd.14853. Online ahead of print.

ABSTRACT

This study tested whether subcutaneous fat loss is enhanced via application of a popular topical fat loss lotion. Twenty resistance-trained men (mean ±SD age of 26.3±6.3 years and weight of 86.8±11.1 kg) had a topical fat-loss lotion containing Coleus forskholii, Silybin, Eucommia ulmoides leaf, Paullinia cupana seed, caffeine and black pepper essential oil applied twice daily for 8 weeks to the front and lateral thigh of one of the participant’s leg, and a placebo control lotion was applied to the same sites on the other leg. After 8 weeks, there were no significant differences between the placebo and treatment legs for the change in subcutaneous fat thickness of the front thigh (P=0.73) or for leg fat percentage (P=0.52). However, there was a slight, yet significant difference in the change in subcutaneous fat thickness of the lateral thigh favouring the treatment leg (-0.42 vs +0.75 mm, P=0.029), but with this difference disappearing depending on the statistical tests being used. Only 2/19 participants perceived a difference in fat loss in response to each condition. Although the topical lotion tested here resulted in statistically significantly greater subcutaneous fat loss at the lateral but not front thigh, this effect was very small, contingent upon the statistical test being used, and unperceivable by the participants themselves.

PMID:35174607 | DOI:10.1111/jocd.14853

J Cosmet Dermatol. 2022 Feb 16. doi: 10.1111/jocd.14852. Online ahead of print.

ABSTRACT

BACKGROUND: Acne vulgaris is a multifactorial disease that mostly heals by scarring. Interleukin-1 beta (IL-1β) is a proinflammatory cytokine, suggested to play a key role in acne pathogenesis.

OBJECTIVE: To study the immunohistochemical (IHC) expression of IL1β in acne vulgaris and acne scars to evaluate its possible role in their pathogenesis and to study the relation between expression of IL1β and the clinicopathological parameters.

PATIENTS AND METHODS: This study was conducted on sixty subjects (twenty patients with acne vulgaris and twenty patients with acne scars), and twenty healthy volunteers as controls. Skin biopsies were taken from patients and controls for routine histopathological examination with Hematoxylin and Eosin (H&E) stain and IHC staining of IL-1β.

RESULTS: There was a statistically significant increase in expression of IL-1β in acne vulgaris compared to post-acne scars and controls, (p<0.001) for both. IL-1β expression was significantly positively correlated with both clinical severity of acne vulgaris (p=0.022) and severity of histopathological inflammation (p=0.011).

CONCLUSION: IL-1β expression was associated with acne vulgaris and post acne scars with significant positive correlation to clinical and histopathological severity of acne vulgaris. Thus IL-1β could be a key player cytokine in acne pathogenesis, its severity and development of post acne scars.

PMID:35174608 | DOI:10.1111/jocd.14852

J Appl Microbiol. 2022 Feb 17. doi: 10.1111/jam.15494. Online ahead of print.

ABSTRACT

AIMS: There are knowledge gaps regarding STEC and EPEC strains in livestock in Jiangsu, China. This study aimed to evaluate the potential public health significance of STEC and EPEC strains isolated from livestock by determining the serotypes, virulence profiles, and genetic relationship with international STEC strains.

METHODS AND RESULTS: A total of 68 STEC and 37 EPEC strains were obtained from 231 fecal sheep samples and 70 fecal cattle samples. By using whole-genome sequencing (WGS) analysis, all STEC belonged to 15 O:H serotypes and the most prevalent serotypes were O6:H10 (19.1%), O155:H21 (14.7%), and O21:H25 (10.3%). The main Shiga toxin gene subtypes detected were stx1c (41.2%), stx1a (26.5%), stx2b (14.7%) and stx2k (14.7%). Only the STEC from cattle carried eae gene. Other adherence-associated or toxin-related genes, including lpfA (70.6%), iha (48.5%), subA (54.4%), and ehxA (33.8%), were found in STEC. All EPEC strains were bfpA-negative, and the predominant eae variants were eae-β1 (62.2%), eae-ζ (21.6%), and eae-θ (8.1%). The core-genome multi-locus sequence typing (cgMLST) analysis revealed nine scattered clusters in STEC and one dominant cluster in EPEC. The strains with the same serotypes, including O22:H8 and O43:H2 in the two towns, possessed a closely genomic distance. The core genome single nucleotide polymorphism (cgSNP) showed that part of STEC strains in this study were clustered with isolates possessing the same serotypes from the Netherlands, Sweden, and Xinjiang of China. Five serotypes of STEC isolates were associated with the clinical STEC strains from databases.

CONCLUSION: This study provided the diverse serotypes and the virulence genes profiles in STEC and EPEC strains. Local strains possessed widely diverse and scattered clusters by cgMLST. Closely genomic correlation with clinical isolates displayed that part of the STEC strains may threaten to public health.

SIGNIFICANCE AND IMPACT OF THE STUDY: Non-O157 STEC strains act as important pathogens for human infections. This study supports the increased surveillance work of non-O157 STEC rather than just O157 STEC in this region.

PMID:35174586 | DOI:10.1111/jam.15494